Gun-Hee Kim

Publications (2)9.87 Total impact

• Article: Luteolin inhibits adipogenic differentiation by regulating PPARgamma activation.

  Hee-Sook Park, Soon-Hee Kim, Young Sup Kim, Shi Yong Ryu, Jin-Taek Hwang, Hye Jeong Yang, Gun-Hee Kim, Dae Young Kwon, Myung-Sunny Kim
  [show abstract] [hide abstract]
  ABSTRACT: Luteolin (3',4',5,7-tetrahydroxyflavone), a flavonoid, has been known to possess antimutagenic, antitumorigenic, antioxidant, and anti-inflammatory properties. In this study, we investigated the role of luteolin in the regulation of adipogenic differentiation in 3T3-L1 preadipocytes. Luteolin inhibited intracellular triglyceride accumulation in a dose-dependent manner without cytotoxicity. Western blot and reverse transcription-polymerase chain reaction analyses showed that this inhibition was accompanied by attenuated expression of the adipogenic transcription factors: peroxisome proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein alpha. Luteolin inhibited the PPARgamma transactivation stimulated by rosiglitazone, a synthetic agonist, in COS-7 cells and inhibited rosiglitazone-induced adipogenic differentiation in 3T3-L1 cells. These data suggest that luteolin exerts antiadipogenic effects by suppressing adipogenic transcription factors and by inhibiting the transactivation of PPARgamma.
  BioFactors 05/2009; 35(4):373-9. · 4.93 Impact Factor
• Article: Luteolin inhibits adipogenic differentiation by regulating PPARγ activation

  Hee-Sook Park, Soon-Hee Kim, Young Sup Kim, Shi Yong Ryu, Jin-Taek Hwang, Hye Jeong Yang, Gun-Hee Kim, Dae Young Kwon, Myung-Sunny Kim
  [show abstract] [hide abstract]
  ABSTRACT: Luteolin (3′,4′,5,7-tetrahydroxyflavone), a flavonoid, has been known to possess antimutagenic, antitumorigenic, antioxidant, and anti-inflammatory properties. In this study, we investigated the role of luteolin in the regulation of adipogenic differentiation in 3T3-L1 preadipocytes. Luteolin inhibited intracellular triglyceride accumulation in a dose-dependent manner without cytotoxicity. Western blot and reverse transcription-polymerase chain reaction analyses showed that this inhibition was accompanied by attenuated expression of the adipogenic transcription factors: peroxisome proliferator-activated receptor (PPAR) and CCAAT/enhancer-binding protein . Luteolin inhibited the PPAR transactivation stimulated by rosiglitazone, a synthetic agonist, in COS-7 cells and inhibited rosiglitazone-induced adipogenic differentiation in 3T3-L1 cells. These data suggest that luteolin exerts antiadipogenic effects by suppressing adipogenic transcription factors and by inhibiting the transactivation of PPAR. © 2009 International Union of Biochemistry and Molecular Biology, Inc.
  BioFactors 04/2009; 35(4):373 - 379. · 4.93 Impact Factor