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ABSTRACT: Pachyman based nanoparticles loading salicylic acid as model drug (SA-PNPs) were prepared by an inverse microemulsion crosslinking
approach using epichlorohydrin (ECH) as crosslinker. The effects of crosslinking reaction time, initial volume ratio of oil
to aqueous phase and dosage of crosslinker on the particle size of SA-PNPs were optimized by orthogonal experimental design.
SA-PNPs prepared under the optimal conditions had the average size of 230 nm and high encapsulation efficiency of 90%. The
in vitro drug release was also investigated and the release data were analyzed using zero order, first order and Higuchi’s
kinetics model. According to the determined coefficients, release data fitted to Higuchi’s model, which suggested that the
release of SA from SA-PNPs in phosphate buffer (pH 7.4) was diffusion controlled release. The experimental results indicated
that pachyman possesses a promising potential to be applied as nanocarriers for controlled drug release.
Key wordsnanoparticles–pachyman–salicylic acid
Journal of Wuhan University of Technology-Mater Sci Ed 04/2012; 26(4):606-610. · 0.35 Impact Factor
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ABSTRACT: Cowpea mosaic virus (CPMV) was analyzed by CZE and the two separated subcomponents recorded at approximately 2.2 and approximately 2.7 min were respectively designated to be the slow electrophoretic form of CPMV (CPMV(s)) and the fast electrophoretic form of CPMV (CPMV(f)), the two electrophoretic forms of CPMV, by the following methods: first, CZE analysis of the biospecific complexes of CPMV and anti-CPMV polyclonal antibody proved the properties of CPMV; second, isolation and CZE analysis of CPMV(f) and CPMV(s). Because CPMV(s) was precipitated and CPMV(f) was left in the supernatant at pH 5.6, the only one peak at approximately 2.7 min from the supernatant was assigned for CPMV(f), while the peak at approximately 2.2 min dominating for the precipite was deemed to be CPMV(s). Third, viral characterization by directly analyzing the capsid proteins using MALDI-TOF-MS. Based on the theoretical molecular weights calculated from the sequence of the capsid proteins, the three peaks at m/z 21 516.75, 23 745.8 and 42 509.22 for natural CPMV were destined for the small (S) protein of CPMV(f), the S protein of CPMV(s) and the large (L) protein of both of them, respectively. As expected, the non-appearance of the peak at m/z 23 745.8 for the isolated CPMV(f) sample indicated the absence of CPMV(s), and the peak at m/z 23 745.8 was predominant in the spectrum for the precipitated CPMV(s) sample. After the confirmation of CPMV(s) and CPMV(f), the CZE separation of them was optimized. The developed analysis method has proven useful in investigating the stability of CPMV and the effect of 2,4-dinitrophenyl-decoration on the modification of the electrophoretical behavior of CPMV.
Electrophoresis 05/2009; 30(9):1572-8. · 3.30 Impact Factor
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ABSTRACT: Linear pachyman was isolated from the sclerotium of Poria cocos. Its hydroxypropyl and carboxymethyl derivatives, with considerable hydrophilicities, were synthesized. Their chemical structures, biocompatibilities, powder and tableting properties were determined. To exploit their novel applications in tablet excipients, ampicillin and probenecid dispersible tablets were compressed using the two derivatives as disintegrants, respectively. The detailed characterization of the tablets indicated the great potential of the two synthesized derivatives to be used as pharmaceutical excipients. Copyright © 2007 John Wiley & Sons, Ltd.
Polymers for Advanced Technologies 03/2007; 18(4):268 - 274. · 2.01 Impact Factor
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ABSTRACT: The microbial transformation of androst-4-ene-3,17-dione (I) by the fungus Beauveria bassiana CCTCC AF206001 has been investigated using pH 6.0 and 7.0 media. Two hydroxylated metabolites were obtained with the pH 6.0 medium. The major product was 11alpha-hydroxyandrost-4-ene-3,17-dione (II) whereas the minor product was 6beta,11alpha-dihydroxyandrost-4-ene-3,17-dione (III). On the other hand, four hydroxylated and/or reduced metabolites were obtained with the pH 7.0 medium. The major product was 11alpha,17beta-dihydroxyandrost-ene-3-one (V) and the minor products were 17beta-hydroxyandrost-ene-3-one (IV), 6beta,11alpha,17beta-trihydroxyandrost-ene-3-one (VI) and 3alpha,11alpha,17beta-trihydroxy-5alpha-androstane (VII). The products were purified by chromatographic methods, and were identified on the basis of spectroscopic methods. This fungus strain is clearly an efficient biocatalyst for 11alpha-hydroxylation and reduction of the 17-carbonyl group.
Steroids 12/2006; 71(11-12):979-83. · 2.83 Impact Factor
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Synthetic Communications 01/2006; 36(6):693-699. · 1.06 Impact Factor
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ABSTRACT: In this investigation, the fabrication, physico-chemical and biological characterization of a novel smart hydrogel had been evaluated for its potentials in effective controlling protein delivery. The hydrophilic pachyman-based hydrogel was generated facilely by crosslinking hydrosoluble carboxymethyl pachyman (CMP) with epichlorohydrin (ECH). The ECH concentration possessing maximum (99.7%) encapsulation efficiency and the most appropriate swelling characteristics was found to be 1.25% (w/v). The resultant hydrogel exhibited swelling ratios most favorable for drug release in simulated intestinal media. It could release two model protein drugs (bovine serum albumin and lysozyme) in the controlled manner and with full preservation of the protein stability and enzymatic activity. Importantly, the ECH-CMP hydrogel was confirmed to be biocompatible and biodegradable. From these findings, we were able to conclude that the synthesized pachyman-based hydrogel would be a promising delivery carrier candidate for site-specific delivery of protein drugs.
Carbohydrate Polymers.
