[Show abstract][Hide abstract] ABSTRACT: Since 1986, the reference of birth weight for gestational age has not been updated. The aim of this study was to set up Chinese neonatal network to investigate the current situation of birth weight in China, especially preterm birth weight, to develop the new reference for birth weight for gestational age and birth weight curve.
A nationwide neonatology network was established in China. This survey was carried out in 63 hospitals of 23 provinces, municipalities and autonomous regions. We continuously collected the information of live births in participating hospitals during the study period of 2011-2014. Data describing birth weight and gestational age were collected prospectively. Newborn's birth weight was measured by electronic scale within 2 hours after birth when baby was undressed. The evaluation of gestational age was based on the combination of mother's last menstrual period, ultrasound in first trimester and gestational age estimation by gestational age scoring system.
the growth curve was drawn by using LMSP method, which was conducted in GAMLSS 1.9-4 software package in R software 2.11.1.
A total of 159 334 newborn infants were enrolled in this study. There were 84 447 male and 74 907 female. The mean birth weight was (3 232 ± 555) g, the mean birth weight of male newborn was (3 271 ± 576) g, the mean weight of female newborn was (3 188 ± 528) g. The test of the variables' distribution suggested that the distribution of gestational age and birth weight did not fit the normal distribution, the optimal distribution for them was BCT distribution. The Q-Q plot test and worm plot test suggested that this curve fitted the distribution optimally. The male and female neonatal birth weight curve was developed using the same method.
Using GAMLSS method to establish nationwide neonatal birth weight curve, and the first time to update the birth weight reference in recent 28 years.
Zhonghua er ke za zhi. Chinese journal of pediatrics 02/2015; 53(2):97-103.
[Show abstract][Hide abstract] ABSTRACT: TLR insufficiency increases newborn's susceptibility to infectious disease.
The peripheral blood of four premature births has been collected weekly from the 28(th) gestational week (GW) until maturity at 36(th) GW. Microarray assays were used to derive dynamic follow-up data of TLR1-10 and other TLR signaling pathway associated factor changes.
The follow-up results showed that the transcription level of TLR1 increased at the 36(th), TLR 3 decreased at the 33(rd) and TLR7 increased at the 34(th) GW significantly, whereas NFkB and its activator TBK1 were highest transcribed in the 28(th) and 32(nd) GW. Low TLR4 transcription in addition to late MD-2 maturation (33(rd) GW) indicated a lack of defense mechanisms against bacterial infections in preterm births particular in the first weeks after birth. Late transcriptional enhancements of TLR1 and MYD88 (35(th) week) as well as β 2 microglobulin (35(th) GW) also indicated a weak immune system in the early maturation stages.
The transcription levels of TLR1, 3, 7 and the signaling pathway associated cofactors were different transcribed during the 28(th) and 36(th) GWs of the premature newborns. In the early stage after preterm birth, beside peak transcriptions of NFkB and TBK1, the immune system is not fully developed and maturation takes place mainly between the 33th and 35(th) GW.
International Journal of Clinical and Experimental Medicine 01/2015; 8(3):4108-14. · 1.42 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Low birth weight is associated with an increased risk of adverse outcomes in many diseases in adult life. We investigated the expression of IGF-II and the status of differentially methylated regions (DMR) in small for gestational age (SGA) infants after birth.
Plasma IGF-II, IGF-II receptor (IGF2R), IGF-I, and IGF-binding protein 3 (IGFBP3) levels were measured after birth in 150 newborn infants. These included 30 term appropriate for gestational age (AGA), 30 term SGA, 30 term large for gestational age (LGA), 30 preterm AGA, and 30 preterm SGA infants.
Plasma IGF-II levels after birth were lower in both term SGA (435.1±33.82 vs. 620.4±44.79, p=0.002) and LGA infants (483.7±33.8 vs. 620.42±44.79, p=0.018) than in term AGA infants. The expression of
IGF-II was associated with birth weight and expressed at high levels, which suggests that IGF-II may continue to play an important role after birth.
[Show abstract][Hide abstract] ABSTRACT: Objectives: To investigate plasma ghrelin and obestatin levels, and ghrelin/obestatin prepropeptide gene polymorphisms, in sequentially enrolled small for gestational age (SGA) infants. Methods: Neonates were sequentially enrolled into this study and were then subdivided into different groups, according to different study aims and availability of study materials. Consequently, plasma ghrelin and obestatin levels were measured in term SGA, term appropriate for gestational age (AGA), term large for gestational age (LGA), preterm SGA and preterm AGA neonates. Levels of both peptides were also measured in AGA infants of different gestational ages, and in term AGA neonates at different days following birth. Three ghrelin/obestatin prepropeptide gene single nucleotide polymorphisms (SNPs), Arg51Gln, Leu72Met, and Gln90Leu, were measured in neonates. Results: The study involved a total cohort of 581neonates. Out of 150 neonates (30 term AGA, 30 term SGA, 30 term LGA, 30 preterm AGA, and 30 preterm SGA), plasma obestatin levels were significantly higher in term SGA versus term LGA neonates (0.21 +/- 0.02 ng/ml versus 0.17 +/- 0.01 ng/ml, respectively). Out of a wider cohort, there were no significant differences in genotypes and allele frequencies of Arg51Gln, Leu72Met, and Gln90Leu SNPs between term SGA and AGA neonates, or between preterm SGA and AGA neonates. Conclusions: Ghrelin/obestatin prepropeptide polymorphisms were not found to be associated with SGA status in neonates; however, ghrelin and obestatin levels may be involved in growth and development. Further studies are required to understand the relationship between ghrelin, obestatin and prenatal development.
The Journal of international medical research 09/2014; 42(6). DOI:10.1177/0300060514533525 · 1.10 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Background Human toll-like receptor 4 (TLR4) is an important receptor in innate immunity, particularly against gram-negative bacterial infection (GNBI). In our study, we evaluated associations of TLR4 single nucleotide polymorphisms (SNPs) with GNBI in Han Chinese neonates. Patients and Methods Polymorphisms in TLR4 were genotyped in 201 neonates with GNBI and 279 gestational age and birth weight-matched controls without GNBI. Polymorphism analyses were applied to allele frequencies of the detected TLR4 SNPs and their associations with various clinical entities, including premature birth and GNBI were assessed. Results A total of six SNPs with more than 5% frequency were found in several promoter sequences, including rs10759931, rs2737190, rs10116253, rs10983755, rs1927914, and rs10759932. Mutation allele frequencies ranged from 23 to 41%. There were no SNPs with a frequency greater than 5% in exon analyses. Allele G rs2737190 mutations and GGCGGC haplotypes were more frequent among preterm GNBI neonates (odds ratio [OR], 1.32; 95% confidence interval [CI], 1.02-1.71 and OR, 1.89; 95% CI, 1.19-3.00, respectively). No specific alleles or haplotypes were associated with GNBI status among term neonates. Conclusion In this study population of Han Chinese, there was a significant association between an ethnical unique SNP in the TLR4 promoter region and preterm neonatal GNBIs.
American Journal of Perinatology 09/2014; 32(04). DOI:10.1055/s-0034-1387929 · 1.60 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Objectives: Tryptophan not only is an amino acid essential to protein synthesis but also serves as a precursor in 2 important metabolic pathways: the serotonin and the kynurenine pathways. Tryptophan is related to sleeping patterns. The objective of the present study was to determine the tryptophan requirement of term infants using the indicator amino acid oxidation (IAAO) method with L-[1-C-13] phenylalanine as the indicator. Methods: Enterally fed infants were randomly assigned to tryptophan intakes ranging from 0.5 to 73 mg . kg(-1) . day(-1) as part of an elemental diet. After 1-day adaptation to the test diet, [C-13]bicarbonate and L-[1-C-13]phenylalanine tracers were given enterally. Breath samples were collected at baseline and during isotopic plateaus. The mean tryptophan requirement was determined by using the biphasic linear regression crossover analysis on the fraction of 13 CO2 recovery from L-[1-C-13]phenylalanine oxidation ((FCO2)-C-13). Data are presented as mean +/- standard deviation. Results: A total of 30 term neonates (gestational age 39 +/- 1 weeks) were studied at 9 +/- 4 days. (FCO2)-C-13 decreased until a tryptophan intake of 15 mg . kg(-1) . day(-1); additional increases in tryptophan intake did not affect F-13 CO2. Mean requirement was determined to be 15 mg . kg(-1) . day(-1). Conclusions: The mean tryptophan requirement for elemental formula-fed term infants is 15 mg .kg(-1) . day(-1). This requirement is lower than the present recommended intake of 29 mg . kg(-1) . day(-1), which is based on the average intake of a breastfed infant.
Journal of Pediatric Gastroenterology and Nutrition 09/2014; 59(3):374-379. DOI:10.1097/MPG.0000000000000434 · 2.87 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Sparse representation classification method has been increasingly used in the fields of computer vision and pattern analysis, due to its high recognition rate, little dependence on the features, robustness to corruption and occlusion, and etc. However, most of these existing methods aim to find the sparsest representations of the test sample y in an overcomplete dictionary, which do not particularly consider the relevant structure between the atoms in the dictionary. Moreover, sufficient training samples are always required by the sparse representation method for effective recognition. In this paper we formulate the classification as a group-structured sparse representation problem using a sparsity-inducing norm minimization optimization and propose a novel sparse representation-based automatic target recognition (ATR) framework for the practical applications in which the training samples are drawn from the simulation models of real targets. The experimental results show that the proposed approach improves the recognition rate of standard sparse models, and our system can effectively and efficiently recognize targets under real environments, especially, where the good characteristics of the sparse representation based classification method are kept.
[Show abstract][Hide abstract] ABSTRACT: This paper is aiming at applying sparse representation based classification (SRC) on face recognition with disguise or illumination variation. Having analyzed the characteristics of general object recognition and the principle of the classifier of SRC method, authors focus on evaluating blocks of a probe sample and propose an optimized SRC method based on position-preserving weighted block and maximum likelihood model. Principle and implementation of the proposed method have been introduced in the article, and experiments on Yale and AR face database have been given too. From experimental results, it can be seen that the proposed optimized SRC method works well than existing methods.
[Show abstract][Hide abstract] ABSTRACT: Objetivo
validar de forma prospectiva um nomograma de bilirrubina transcutânea (BTc) para identificar hiperbilirrubinemia grave em neonatos a termo e pré‐termo tardios saudáveis na China.
foi realizado um estudo multicêntrico que incluiu 9174 neonatos a termo e pré‐termo tardios saudáveis em oito unidades da China. Foram realizadas dosagens de BTc utilizando um bilirrubinômetro. Os valores de BTc foram traçados em um nomograma de BTc para identificar a capacidade de predição de hiperbilirrubinemia significativa.
972 recém‐nascidos (10,6%) desenvolveram hiperbilirrubinemia significativa. O percentil 40 de nosso nomograma pode identificar todos os recém‐nascidos com risco de hiperbilirrubinemia significativa, porém com baixo valor preditivo positivo (VPP) (18,9%). De 453 recém‐nascidos acima do percentil 95, 275 recém‐nascidos desenvolveram posteriormente hiperbilirrubinemia significativa, com VPP elevado (60,7%), porém com baixa sensibilidade (28,3%). O percentil de 75 foi altamente específico (81,9%) e moderadamente sensível (79,8%). A área sob a curva (ASC) de nosso nomograma de BTc foi de 0,875.
este estudo validou o nomograma de BTc, que pode ser utilizado para prever hiperbilirrubinemia significativa em neonatos a termo e pré‐termo tardios saudáveis na China. Contudo, combinar o nomograma de BTc e fatores de risco clínicos pode melhorar a precisão de predição da hiperbilirrubinemia grave, o que não foi avaliado neste estudo. São necessários estudos adicionais para confirmar essa combinação.
Jornal de pediatria 05/2014; 90(3). DOI:10.1016/j.jped.2013.08.013 · 0.94 Impact Factor