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ABSTRACT: Limbal epithelial stem cells are the ultimate source of regeneration of the entire corneal epithelium under both normal and injured conditions. The corneal epithelium plays a crucial role in homeostasis and integrity of the eye. To maintain the integrity of the ocular surface, corneal epithelial cells must be balanced by stem cells, located at the limbus. The limbus is the crossing area between the cornea and sclera, 1 mm in width, and together with conjunctival epithelium plays an important role in regenerating the cornea after traumatic injuries. The aim of this study was to evaluate the culturing patterns of canine limbal stem cells and to optimize growing conditions of these cell cultures in order to develop a reliable biomedical model intended for studying the potentials of allografts/xenografts originated from canine tissues. Canine stem cell equivalents have potentials in reparative/regenerative veterinary medicine.
Acta Veterinaria. 01/2010;
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ABSTRACT: Magnetic resonance as a part of a broad approach to seizures in dog -two cases of hydrocephalus in dogs with cluster seizures. Vet. arhiv 77, 377-386, 2007. ABSTRACT This paper describes two cases of hydrocephalus in dog. Both dogs were presented at the Clinic for Internal Diseases, Faculty of Veterinary Medicine, Zagreb, after having experienced cluster seizures and status epilepticus, respectively. In each dog physical and neurological examination was performed, revealing no abnormalities. Complete laboratory workup and urinalysis were included for each dog and all results were within reference range. Both dogs underwent magnetic resonance (MR) imaging and through this procedure a diagnosis of hydrocephalus was established. Anticonvulsive therapy and therapy against increased intracranial pressure was introduced in both dogs. Treatment resulted in marked clinical improvement.
Veterinarski Arhiv 01/2007; 77:377-386.
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ABSTRACT: MRLJAK, V., R. BARIĆ RAFAJ, V. SUŠIĆ, V. MATIJATKO, N. KUČER, I. KIŠ: Antithrombin III in healthy dogs and in dogs suffering from babesiosis. Vet. arhiv 75, 477-486, 2005. ABSTRACT Antithrombin III (AT III) was measured in 15 dogs with Babesia canis infection and in 15 healthy dogs. Blood samples for control group and patients were taken before the therapy with imidocarb dipropionat and on the 1st and 5th days after the therapy. Measurements of AT III activity in canine plasma with thrombin dependent chromogenic substrate assay using automatic analyzer showed a good within-run precision. The results were expressed as proportions of the norm. Value for AT III before the therapy was lower than in the control group. Activity of AT III was higher on the post-treatment day 5 than before the therapy. A survey of individual data demonstrated that in 2 dogs the pre-treatment AT III values were 0.66 and 0.79, respectively, showing consumption inhibitors of coagulation. Introduction Canine babesiosis is a tick-borne disease of worldwide importance, ranging in severity from relatively mild to fatal (LOBETTI, 1998). The causative organism of canine babesiosis is the intraerythrocytic protozoan parasite, either Babesia canis or Babesia gibsoni. Three subtypes or strains of B. canis are recognized, namely B. canis canis, B. canis vogeli and B. canis rossi (LOBETTI, 1998; SHAW et al., 2001).
01/2005; 75:477-486.
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Nada Kučer,
Vesna Matijatko, Ivana Kiš,
Darko Grden,
Mirna Brkljačić,
Jadranka Foršek,
Zdravko Žvorc,
Renata Barić Rafaj,
N Kučer,
V Matijatko,
I Kiš,
D Grden,
M Brkljačić,
J Foršek,
Z Žvorc,
R Barić Rafaj
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ABSTRACT: White blood cell count and neutrophil to lymphocyte ratio in uncomplicated and complicated canine babesiosis caused by Babesia canis canis. Vet. arhiv 78, 321-330, 2008. aBstRact Canine babesiosis caused by B. canis is very common cause of morbidity and mortality of dogs in Croatia. Although white blood cell populations play a crucial role in the SIRS it has been poorly described in canine babesiosis. Therefore the aim of this study was to investigate serial changes in circulating total white blood cells and relationship between circulating neutrophil and lymphocyte counts in uncomplicated and complicated canine babesiosis caused by B. canis canis. The study was performed on two groups of animals naturally infected by B. Canis canis. Group 1 consisted of 30 dogs with uncomplicated babesiosis while group 2 consisted of 30 dogs with various complications. The blood samples for the analyses were collected at the day of the admission, and the second and seventh day after the administration of imidocarb dipropionat. White blood cell count (WBC) was determined using an automatic haematology analyzer (System 9120; Serono Baker Diagnostic, Pennsylvania, USA). The ratio of neutrophil to lymphocyte count was acquired from relative percentages of neutrophils and lymphocytes that were manually determined from Romanowsky stained blood smears. The percentage of parasitemia was determined by counting the number of parasitized erythrocytes on one thousand erythrocytes. The differential count was determined manually in Romanovsky-stained blood smears. The WBC counts, relative number of neutrophils and lymphocytes, neutrophil to lymphocyte ratio and levels of parasitemia were compared between the two groups using the Mann-Whitney U test. White blood cell count was significantly higher on the seventh day after the antibabesial treatment in the group of complicated babesiosis. The relative number of neutrophiles was significantly higher on the second and seventh days after the antibabesial treatment in complicated babesiosis and the relative number of lymphocytes was significantly lower in the group of complicated babesiosis on the second day after the antibabesial treatment. The neutrophil to lymphocyte ratio was significantly higher on the second day after the treatment in the complicated babesiosis.
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ABSTRACT: KIš, I., J. ForšeK, V. MatIJatKo, N. Kučer, K. šIMoNJI: Steroid-responsive Steroid-responsive meningitis-arteritis in a dog -a case report�� �et�� ar�iv ���� ���-����� ������ �� �et�� ar�iv ���� ���-����� ������ �et�� ar�iv ���� ���-����� ������ AbStrAct This paper describes in detail the case of steroid-responsive meningitis-arteritis (SRMA) in a dog. The dog was presented at the Clinic for Internal Diseases due to high fever with anorexia that lasted for five days, reluctance to move, hunched stance and stiffness that responded only moderately to antibiotics and a combination of steroidal and non-steroidal anti-inflammatory drugs administered by the referring veterinarian. The dog was fully clinically and neurologically examined and revealed fever and extreme neck pain. Complete laboratory workup and urinalysis were included. The dog underwent cerebrospinal fluid (CSF) sampling and analysis. The diagnosis of steroid-responsive meningitis-arteritis was established. Adequate therapy was instituted which resulted in clinical resolution of the disease.
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ABSTRACT: The records of all canine patients (86) that had been diagnosed with babesiosis and that were admitted to the Clinic for Internal Diseases, Faculty of Veterinary Medicine, Zagreb from January 2007 to December 2007 were reviewed retrospectively. All dogs that had been diagnosed with canine babesiosis and that had systemic inflammatory response syndrome (SIRS) followed by multiple organ dysfunction syndrome (MODS), and refractory hypotension, were included in this study. Of 86 patients diagnosed with canine babesiosis that were admitted during the study period, 10 had evidence of septic shock and were included in this study. Seven of the 10 dogs had a level of parasitaemia above 1%, with the highest level being 20.2%, seven of the 10 dogs were anaemic and three of the 10 dogs were leucopoenic. Thrombocytopenia was present in nine dogs. Hypoglycaemia was noted in two dogs, and bilirubinaemia in nine dogs.Four patients had involvement of two organs, five had involvement of three organs, and one had involvement of four organs. The organ that was most frequently involved was the kidney (nine cases). Central nervous system dysfunction was the rarest complication noted (one case). The mortality rate in non-septic shock canine babesiosis was 2.6%. All dogs that developed septic shock died between the first and the fourth day after admission. The 100% mortality rate that is reported here reflects the fact that in cases in which progression of the inflammatory response leads to the development of septic shock, an unfavourable outcome should be expected.
Veterinary Parasitology.
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ABSTRACT: This paper describes two cases of hydrocephalus in dog. Both dogs were presented at the Clinic for Internal Diseases, Faculty of Veterinary Medicine, Zagreb, after having experienced cluster seizures and status epilepticus, respectively. In each dog physical and neurological examination was performed, revealing no abnormalities. Complete laboratory workup and urinalysis were included for each dog and all results were within reference range. Both dogs underwent magnetic resonance (MR) imaging and through this procedure a diagnosis of hydrocephalus was established. Anticonvulsive therapy and therapy against increased intracranial pressure was introduced in both dogs. Treatment resulted in marked clinical improvement.
Veterinary Archives (veterinarski.arhiv@vef.hr); Vol.77 No.5.
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ABSTRACT: Canine babesiosis caused by B. canis is very common cause of morbidity and mortality of dogs in Croatia. Although white blood cell populations play a crucial role in the SIRS it has been poorly described in canine babesiosis. Therefore the aim of this study was to investigate serial changes in circulating total white blood cells and relationship between circulating neutrophil and lymphocyte counts in uncomplicated and complicated canine babesiosis caused by B. canis canis. The study was performed on two groups of animals naturally infected by B. Canis canis. Group 1 consisted of 30 dogs with uncomplicated babesiosis while group 2 consisted of 30 dogs with various complications. The blood samples for the analyses were collected at the day of the admission, and the second and seventh day after the administration of imidocarb dipropionat. White blood cell count (WBC) was determined using an automatic haematology analyzer (System 9120; Serono Baker Diagnostic, Pennsylvania, USA). The ratio of neutrophil to lymphocyte count was acquired from relative percentages of neutrophils and lymphocytes that were manually determined from Romanowsky stained blood smears. The percentage of parasitemia was determined by counting the number of parasitized erythrocytes on one thousand erythrocytes. The differential count was determined manually in Romanovsky-stained blood smears. The WBC counts, relative number of neutrophils and lymphocytes, neutrophil to lymphocyte ratio and levels of parasitemia were compared between the two groups using the Mann-Whitney U test. White blood cell count was significantly higher on the seventh day after the antibabesial treatment in the group of complicated babesiosis. The relative number of neutrophiles was significantly higher on the second and seventh days after the antibabesial treatment in complicated babesiosis and the relative number of lymphocytes was significantly lower in the group of complicated babesiosis on the second day after the antibabesial treatment. The neutrophil to lymphocyte ratio was significantly higher on the second day after the treatment in the complicated babesiosis. Parasitemia levels were significantly higher in complicated canine babesiosis. On the basis of these results it may be concluded that there is a significant correlation between the severity of clinical course and increase in neutrophils and decrease in lymphocyte percentage. The ratio of neutrophil and lymphocyte counts (in absolute or relative values) is an easily measurable parameter which may express the severity of the affliction and serve as a good prognostic marker.
Veterinary Archives (veterinarski.arhiv@vef.hr); Vol.78 No.4.
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ABSTRACT: The erythrocyte sedimentation rate (ESR), white blood cell count (WBC), haematocrit (HCT) and platelet number (PLT) were quantified and compared with the acute phase proteins (APPs) in dogs naturally infected with Babesia canis and healthy dogs. Both groups were treated with imidocarb dipropionate on the day of admission and both groups were monitored for all parameters on the admission day and on the first, second, third, fourth and seventh days in order to determine the presence of an acute phase reaction, to assess the diagnostic value of these markers in uncomplicated canine babesiosis and to evaluate the use of APPs in treatment monitoring.It was demonstrated that an acute phase response occurs in dogs naturally infected with Babesia canis, with significant increases in the concentration of major acute phase proteins. The serum concentration of C-reactive protein (CRP), serum amyloid A (SAA) and the erythrocyte sedimentation rate (ESR) decreased daily after treatment and approached reference range values by the eighth day. PLT and haematocrit (HCT) increased daily after treatment and approached reference range values by the fourth day. WBC and haptoglobin increased after treatment and then decreased from the third and fourth days, respectively, to the eighth day. The diagnostic sensitivity of CRP, SAA and PLT was significantly higher compared to haptoglobin, ESR, HCT and the WBC count. CRP and SAA were of clinical use in monitoring the response to antibabesial treatment.
Veterinary Parasitology.
