Publications (2)0 Total impact
Article: Measurement of free and bound alcohol metabolites and methanol in human biological samples--free and bound alcohol metabolites and methanol in acute alcohol administration.[show abstract] [hide abstract]
ABSTRACT: There are almost no studies on the in vivo distribution kinetics of free and bound ethanol, alcohol metabolites (acetaldehyde and acetate) or the related substance, methanol, during alcohol oxidation. Thus, an acute alcohol administration experiment (alcohol consumption experiment) was carried out using volunteers (five healthy adult males; 2 flushers, 3 non-flushers), and distribution kinetics were investigated in biological samples (blood and urine). The levels of alcohol metabolites and methanol were measured as free compounds in blood samples and bound and free compounds in urine samples. The results showed an increase over time of free alcohol metabolites in both the flusher and non-flusher groups, followed by a subsequent decrease. In addition, free methanol increased over time. Both bound alcohol metabolites and bound methanol were found to increase over time. Based on these findings, levels of free and bound alcohol metabolites and methanol in the biological samples were found to increase relative to levels before consumption in both the flusher and non-flusher groups. This is thought to be due to the binding of alcohol metabolites and methanol to biological components and increases during ethanol oxidation. It was concluded that this is the mechanism by which ethanol, alcohol metabolites and methanol accumulate in the body as a result of chronic alcohol consumption, suggesting that it may be possible to use these compounds as markers of consumption by measuring these compounds in biological samples taken from alcohol abusers or alcoholics.Nihon Arukōru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence 03/2009; 44(1):13-25.
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ABSTRACT: It is believed that ethanol metabolites (ethanol, acetaldehyde and acetate) are produced when alcohol is consumed in accumulation with the binding of biological components. Additionally, it has been reported that ethanol metabolites and methanol present in the blood and urine are bound to biological components in habitual alcohol drinkers, even when alcohol has not been consumed. Consequently, with the purpose of investigating the potential for effectively using ethanol metabolite and methanol as markers of alcohol abuse, acetaldehyde level was measured in blood hemoglobin samples, and ethanol, acetaldehyde and methanol levels were measured in urine samples in healthy adult males (volunteers: control group) and alcoholics that had not consumed alcohol. Simultaneously, investigations were carried out on the genetic analysis of ADH and ALDH enzymes that participate in ethanol metabolism. Acetaldehyde levels were found to be significantly higher in alcoholics than in the volunteers for the period of 2 to 3 months after admission. In urine samples, acetaldehyde level (bound) was significantly higher in the period of 2 to 3 months after admission, and methanol level (free and bound) was significantly higher within a period of 1 month after admission. A correlation between alcoholics and genotype was found with regard to the distribution of ADH2 and ALDH2 genotypes (Volunteers: ADH2 2-2 type, 81%; ALDH2 1-1 type, 61.9%; Alcoholics: ADH2 2-2 type, 39.6%; ALDH2 1-1 type, 84.9%). Based on these results, alcoholism or alcohol abuse might be predicted by concentration of acetaldehyde as well as methanol during abstinence. It also might be used as markers of alcohol abuse.Nihon Arukōru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence 03/2009; 44(1):26-37.