Philip A Davies

South Australian Research and Development Institute, Tarndarnya, South Australia, Australia

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Publications (2)4.23 Total impact

  • Sue Broughton, Parminder K Sidhu, Philip A Davies
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    ABSTRACT: Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.
    Methods in molecular biology (Clifton, N.J.) 01/2014; 1145:167-89. · 1.29 Impact Factor
  • Parminder K Sidhu, Philip A Davies
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    ABSTRACT: Regeneration of fertile green plants from isolated oat microspores is reported for the first time. Factors critical for microspore growth and regeneration include cold pre-treatment, pH of culture medium and the use of conditioned culture medium. It was found that cold pre-treatment at 4 degrees C in the dark for a minimum of 6 weeks was necessary to consistently achieve microspore growth into multicellular structures (MCS). Longer pre-treatments of up to 9 weeks were tested and found to be positively correlated with the number of MCS produced. Microspore culture medium with pH 8.0 produced significantly more MCS larger than eight cells in size than media with pH 5.8. The use of medium conditioned by actively growing barley microspores significantly increased the numbers of MCS larger than eight cells in size compared to non-conditioned media. Plants were regenerated only from cultures using conditioned medium. A total of 2 green plants and 15 albinos were regenerated. Of the green plants, one had the haploid chromosome complement (n = 3x = 21) and the other had the parental hexaploid chromosome complement (2n = 6x = 42) which may be due to spontaneous chromosome doubling. The hexaploid plant set seed naturally and the haploid plant set seed after its chromosome complement was doubled with colchicine.
    Plant Cell Reports 03/2009; 28(4):571-7. · 2.94 Impact Factor

Publication Stats

7 Citations
4.23 Total Impact Points

Institutions

  • 2009
    • South Australian Research and Development Institute
      Tarndarnya, South Australia, Australia