J P N Rai

G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarkāshi, Uttarakhand, India

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Publications (14)15.3 Total impact

  • Rama Pal, J.P.N. Rai, S.K. Dubey
    4th National Seminar on "Innovative Saline Agriculture in Changing Environment", Rajmata Vijayaraje Scindia Krishi Viswhwavidayalaya, Gwalior, M.P., India; 12/2014
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    ABSTRACT: Two indigenous bacterial strains, Bacillus megaterium ETLB-1 (accession no. KC767548) and Pseudomonas plecoglossicida ETLB-3 (accession no. KC767547), isolated from soil contaminated with paper mill effluent, were co-immobilized on corncob cubes to investigate their biodegradation potential against black liquor (BL). Results exhibit conspicuous reduction in color and lignin of BL upto 913.46 Co-Pt and 531.45 mg l(-1), respectively. Reduction in chlorophenols up to 12 mg l(-1) was recorded with highest release of chloride ions, i.e., 1290 mg l(-1). Maximum enzyme activity for lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (LAC) was recorded as 5.06, 8.13, and 8.23 U ml(-1), respectively, during the treatment. Scanning electron microscopy (SEM) revealed successful immobilization of bacterial strains in porous structures of biomaterial. Gas chromatography/mass spectroscopy (GC/MS) showed formation of certain low molecular weight metabolites such as 4-hydroxy-benzoic acid, 3-hydroxy-4-methoxybenzaldehyde, ferulic acid, and t-cinnamic acid and removal of majority of the compounds (such as teratogenic phthalate derivatives) during the period of treatment. Results demonstrated that the indigenous bacterial consortium possesses excellent decolorization and lignin degradation capability which enables its commercial utilization in effluents treatment system.
    Environmental science and pollution research international. 11/2014;
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    Rama Pal, J P N Rai, S K Dubey
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    ABSTRACT: An experiment was conducted to evaluate the phytoremediation potential of Eichornia crassipes exposed to different dilutions (i.e., 25%, 50% and 75%) of brass and electroplating industry effluent. A supporting experiment was also conducted in parallel with synthetic binary and single metal solutions of Cd and Cr to assess their impact on growth of plants. The maximum removal of heavy metals was observed under 50% dilution effluent (i.e., 89% for Cd and 87% for Cr) followed by 75% (i.e. 81% for Cd and 77% for Cr) and 25% (i.e. 51% for Cd and 50% for Cr) dilutions. Richard's model fitted to shoot growth data showed highest growth and rate in 50% diluted effluent (k=0.372; Kc=0.49 for Cd and 0.45 for Cr). The study suggested that E. crassipes can be a good plant species for remediation of water bodies moderately contaminated with brass and electroplating industry effluent.
    Journal of Soil Salinity and Water Quality. 01/2014; 6((1),):16-20,.
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    ABSTRACT: Biodegradation of α, β, γ and δ hexachlorocyclohexane (HCH) isomers was studied in broth medium and soil microcosm by Bacillus circulans and Bacillus brevis isolated from contaminated soil. Degradation of α and γ isomers by both the bacterial isolates was higher than thermodynamically stable β and δ isomers. However, B. circulans was found more effective than B. brevis for β and δ isomers. Maximum rate of degradation was recorded at 150 mg/L followed by 100 and 50 mg/L. Soil microcosm study revealed maximum degradation of HCH isomers in the treatment containing natural soil, pesticide and bacterial inocula than the treatment having sterilised soil, pesticide and bacterial isolates. Chloride release was positively co-related with HCH degradation in broth medium as well as in soil microcosm, suggesting that B. circulans and B. brevis hold promising potential by having efficient enzyme(s) required for dechlorination of HCH from contaminated sites.
    Chemistry and Ecology 01/2014; 30(2). · 1.07 Impact Factor
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    ABSTRACT: Biosorption of Cu(2+), Zn(2+), and Cr(6+) from aqueous solutions by leaf biomass of Jatropha curcas was investigated as a function of biomass concentration, initial metal ion concentration, contact time, and pH of the solution systematically. The aim of this study was to optimize biosorption process and find out a suitable kinetic model for the metal removal in single and multi-metal system. The experimental data were analyzed using two sorption kinetic models, viz., pseudo-first- and pseudo-second-order equations, to determine the best fit equation for the biosorption of metal ions Cu(2+), Zn(2+), and Cr(6+) onto the leaf biomass of J. curcas in different metal systems. The experimental data fitted well the pseudo-second-order equation and provided the best correlation for the biosorption process. The findings of the present investigation revealed that J. curcas leaf biomass was an eco-friendly and cost-effective biosorbent for the removal of heavy metal ions from wastewater.
    Environmental Monitoring and Assessment 10/2013; · 1.68 Impact Factor
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    ABSTRACT: The equilibrium sorption of Cr(VI) and Cu(II) from aqueous solution using Jatropha curcas deoiled cake, has been studied with respect to adsorbent dosage, contact time, pH, and initial metal concentration in batch mode experiments. Removal of Cu(II) by deoiled cake was greater than that of Cr(VI). The adsorbent chemical characteristics, studied by Fourier transform-infrared analysis, suggested that the presence of Cr(VI) and Cu(II) in the biomass influenced the bands corresponding to hydroxyl and carboxyl groups. Desorption studies revealed that maximum metals recovery was achieved by HNO3 followed by CH3COOH and HCl. The Freundlich isotherm model showed good fit to the equilibrium adsorption data. The adsorption kinetics followed the pseudo-second-order model, which provided the best correlation for the biosorption process, and suggested that J. curcas deoiled cake can be used as an efficient biosorbent over other commonly used sorbents for decontamination of Cr(VI)- and Cu(II)-containing wastewater.
    Water Environment Research 08/2013; 85(8):733-42. · 1.13 Impact Factor
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    ABSTRACT: Chromate-resistant bacterial strain isolated from the soil of tannery was studied for Cr(VI) bioaccumulation in free and immobilised cells to evaluate its applicability in chromium removal from aqueous solution. Based on the comparative analysis of the 16S rRNA gene, and phenotypic and biochemical characterization, this strain was identified as Paenibacillus xylanilyticus MR12. Mechanism of Cr adsorption was also ascertained by chemical modifications of the bacterial biomass followed by Fourier transform infrared spectroscopy analysis of the cell wall constituents. The equilibrium biosorption analysed using isotherms (Langmuir, Freundlich and Dubinin-Redushkevich) and kinetics models (pseudo-first-order, second-order and Weber-Morris) revealed that the Langmuir model best correlated to experimental data, and Weber-Morris equation well described Cr(VI) biosorption kinetics. Polyvinyl alcohol alginate immobilised cells had the highest Cr(VI) removal efficiency than that of free cells and could also be reused four times for Cr(VI) removal. Complete reduction of chromate in simulated effluent containing Cu(2+), Mg(2+), Mn(2+) and Zn(2+) by immobilised cells, demonstrated potential applications of a novel immobilised bacterial strain MR12, as a vital bioresource in Cr(VI) bioremediation technology.
    Environmental Science and Pollution Research 01/2013; · 2.76 Impact Factor
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    ABSTRACT: Bioligninolysis involves living organisms and/or their products in degradation of lignin, which is highly resistant, plant-originated polymer having three-dimensional network of dimethoxylated (syringyl), monomethoxylated (guaiacyl), and non-methoxylated (p-hydroxyphenyl) phenylpropanoid and acetylated units. As a major repository of aromatic chemical structures on earth, lignin bears paramount significance for its removal owing to potential application of bioligninolytic systems in industrial production. Early reports illustrating the discovery and cloning of ligninolytic biocatalysts in fungi was truly a landmark in the field of enzymatic delignification. However, the enzymology for bacterial delignification is hitherto poorly understood. Moreover, the lignin-degrading bacterial genes are still unknown and need further exploration. This review deals with the current knowledge about ligninolytic enzyme families produced by fungi and bacteria, their mechanisms of action, and genetic regulation and reservations, which render them attractive candidates in biotechnological applications.
    Applied biochemistry and biotechnology 05/2012; 167(7):1865-89. · 1.94 Impact Factor
  • Monica Rawat, J. P. N. Rai
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    ABSTRACT: The present investigation was carried out to isolate bacterial strains from soil/mud samples of metal-polluted environment to check whether the natural adaptation of microbes has equipped them for bioremediation of toxic heavy metals. The primary and secondary screening resulted in 50 mesophilic autotrophic isolates of microbial consortium adapted for metal tolerance and bioadsorption potentiality. The multimetal tolerance in bacterial strain was developed by sequential transfer to higher concentrations of Cd, Cr, Cu, Pb, Ni, and Zn. The isolates were checked for their biosolubilization potential with copper-containing metal sulfide ores, viz. chalcopyrite exhibited 64% and covellite 54% solubilization in the presence of 10 M multiple heavy metals on the fifth day at 35°C and pH 6.0. Metal adsorption of highly potential isolate, i.e., Paenibacillus validus MP5, studied by inductively coupled plasma optical emission spectroscopy (ICP-OES), showed maximum adsorption of Zn 27%, followed by Ni and Cd 16%, Cr 15%, Co 9%, and Pb 7.5% in chalcopyrite, which suggested its possible role in decontamination of metal-polluted sites.
    Bioremediation Journal - BIOREMEDIAT J. 01/2012; 16(2):66-73.
  • Krishna Giri, J. P. N. Rai
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    ABSTRACT: Bacterial isolates from endosulfan-contaminated soil were grown in minimal medium and screened for endosulfan degradation. The isolate which used endosulfan and showed maximum growth was selected for detailed study. Maximum degradation in shake flask culture by Pseudomonas fluorescens was 92.80% of α and 79.35% of β endosulfan isomers in 15 days at 20 mg/L concentration, followed by 50 and 100 mg/L, while the corresponding values in static condition were 69.15 and 51.39%, respectively. Endosulfan concentration degradation declined significantly at 50 and 100 mg/L. Concomitant to degradation, release of chloride ion exhibited positive relation, while pH decreased from 7.0 to 4.53 in agitating and 7.0–5.18 in static condition. The soil microcosm study revealed maximum endosulfan degradation in sterilized soil amended with P. fluorescens. Endosulfan diol and endosulfan ether were among the products of endosulfan metabolism in broth culture, but only endosulfan ether was detected in the soil microcosm. Endosulfan sulphate, a persistent and toxic metabolite of endosulfan, was not detected in either case. The study showed that P. fluorescens could be used effectively for bioremediation of the pesticide contaminated sites.
    International Journal of Environmental Studies 01/2012;
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    Krishna Giri, J P N Rai
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    ABSTRACT: Bacterial isolates from endosulfan-contaminated soil were grown in minimal medium and screened for endosulfan degradation. The isolate which used endosulfan and showed maximum growth was selected for detailed study. Maximum degradation in shake flask culture by Pseudomonas fluores-cens was 92.80% of α and 79.35% of β endosulfan isomers in 15 days at 20 mg/L concentration, followed by 50 and 100 mg/L, while the corresponding values in static condition were 69.15 and 51.39%, respectively. Endosulfan concentration degradation declined significantly at 50 and 100 mg/L. Concomitant to degradation, release of chloride ion exhibited positive relation, while pH decreased from 7.0 to 4.53 in agitating and 7.0–5.18 in static condition. The soil microcosm study revealed maximum endosulfan degradation in sterilized soil amended with P. fluorescens. Endosul-fan diol and endosulfan ether were among the products of endosulfan metabolism in broth culture, but only endosulfan ether was detected in the soil microcosm. Endosulfan sulphate, a persistent and toxic metabolite of endosulfan, was not detected in either case. The study showed that P. fluo-rescens could be used effectively for bioremediation of the pesticide contaminated sites.
  • Rama Pal, J. P.N. Rai
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    ABSTRACT: The phytoextraction potential of water hyacinth (Eichchornia crassipes) was assessed for the removal of selenium and copper individually and from binary solutions. Plant growth, estimated on day 16 of metal treatment, decreased at all concentrations of selenium (2–12 ppm), whereas it increased at lower concentrations of copper (4–12 ppm) and decreased at higher exposure levels. Unlike copper, the rate constant for selenium uptake and its accumulation factors, calculated for both root and shoot, were lower in a binary solution than in the corresponding single-metal solution. Analysis of the elemental composition of the plant revealed that in single-metal copper treatment, the level of Mg decreased with a higher magnitude, followed by K and Ca. However, in the case of selenium, the Ca level increased, Mg remained unaffected and the K level decreased with increasing exposure. Our results revealed that a water hyacinth-based system could successfully remove selenium and copper from water/wastewater.
    Chemistry and Ecology 06/2010; 26(3):163-172. · 1.07 Impact Factor
  • Rama Pal, Saumyata Tewari, Jai P N Rai
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    ABSTRACT: The dead Kluyveromyces marxianus biomass, a fermentation industry waste, was used to explore its sorption potential for lead, mercury, arsenic, cobalt, and cadmium as a function of pH, biosorbent dosage, contact time, agitation speed, and initial metal concentration. The equilibrium data fitted the Langmuir model better for cobalt and cadmium, but Freundlich isotherm for all metals tested. At equilibrium, the maximum uptake capacity (Qmax) was highest for lead followed by mercury, arsenic, cobalt, and cadmium. The RL values ranged between 0-1, indicating favorable sorption of all test metals by the biosorbent. The maximum Kf value of Pb showed its efficient removal from the solution. However, multi-metal analysis depicted that sorption of all metals decreased except Pb. The potentiometric titration of biosorbent revealed the presence of functional groups viz. amines, carboxylic acids, phosphates, and sulfhydryl group involved in heavy metal sorption. The extent of contribution of functional groups and lipids to biosorption was in the order: carboxylic>lipids>amines>phosphates. Blocking of sulfhydryl group did not have any significant effect on metal sorption.
    Biotechnology Journal 06/2009; 4(10):1471-8. · 3.71 Impact Factor
  • Rama Pal, J P N Rai
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    ABSTRACT: Phytochelatins (PCs) are enzymatically synthesized peptides known to involve in heavy metal detoxification and accumulation, which have been measured in plants grown at high heavy metal concentrations, but few studies have examined the response of plants even at lower environmentally relevant metal concentrations. Recently, genes encoding the enzyme PC synthase have been identified in plants and other species enabling molecular biological studies to untangle the mechanisms underlying PC synthesis and its regulation. The present paper embodies review on recent advances in structure of PCs, their biosynthetic regulation, roles in heavy metal detoxification and/or accumulation, and PC synthase gene expression for better understanding of mechanism involved and to improve phytoremediation efficiency of plants for wider application.
    Applied biochemistry and biotechnology 03/2009; 160(3):945-63. · 1.94 Impact Factor