Publications (2)2.27 Total impact
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ABSTRACT: Strain SLG5B-19(T), isolated from an oil-polluted saline soil in Gudao in the coastal Shengli Oilfield, eastern China, was Gram-negative with monoprosthecae or bipolar prosthecae and buds on the prosthecal tips. Growth occurred at NaCl concentrations between 0 and 7 % (w/v), at temperatures between 4 and 45 degrees C, and at pH 6.0-9.0. Strain SLG5B-19(T) had Q-9 as the major respiratory quinone and unsaturated C(18 : 1)omega7c as the predominant cellular fatty acid. The G+C content of the genomic DNA was 59.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SLG5B-19(T) belonged to a clade with the genera Filomicrobium and Hyphomicrobium in the class Alphaproteobacteria. However, 16S rRNA gene sequence similarities of strain SLG5B-19(T) to the phylogenetically most closely related strains, i.e. the type strains of Filomicrobium fusiforme and Hyphomicrobium zavarzinii, were 95.8 and 94.5 %, respectively. In addition, the 16S rRNA gene sequence of strain SLG5B-19(T) had 24 signature nucleotides that were identical to those of the type strain of F. fusiforme. Based on phylogenetic analysis of 16S rRNA gene sequences, strain SLG5B-19(T) could be allocated to the genus Filomicrobium. However, distinct phenotypic differences were observed between strain SLG5B-19(T) and the type strain of F. fusiforme. It is therefore proposed that strain SLG5B-19(T) represents a novel species in the genus Filomicrobium, Filomicrobium insigne sp. nov. The type strain is SLG5B-19(T) (=CGMCC 1.6497(T)=LMG 23927(T)).International journal of systematic and evolutionary microbiology 03/2009; 59(Pt 2):300-5. · 2.27 Impact Factor
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ABSTRACT: Fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes was applied for analyzing the structure of sulfate reducing prokaryotes (SRPs) community in injection water of Shengli Oil Field. Eight probes and their various combinations were used to detect SRPs in the water. Results showed SRPs detected in the water were diverse, which followed in 4 bacterial phyla and 1 archaeal phylum. Total amount of SRPs was 2.86 x 10(4) cells/mL, accounting for 20% of total cells of microorganisms in the water of the Oil Field. Desulfovibrio and Desulfotomaculum cells were about 8.71% (+/- 4.45%) and 12.15% (+/- 3.90%) of the total microbial cells respectively, being dominant in the water. The relative amounts of SRPs belonging to Desulfobacterales and Syntrophobacterales, Thermodesulfobacteriales, and Thermodesulfovibro to total microbial cells in the water were 7.59% (+/- 2.92%), 3.57% (+/- 1.39%) and 2.32% (+/- 0.80%) respectively. In addition, SRPs belonging to Archaeoglobus were also detected with the amount of 4.29% (+/- 1.75%) of total microbial cells, which tells that archeal SRPs are also very important sulfate reducing microorganisms in the injection water of oil field.Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.] 06/2006; 27(5):972-6.