Hailin Zhang

Yunnan Center for Disease Control and Prevention, Yün-nan, Yunnan, China

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Publications (9)31.59 Total impact

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    ABSTRACT: To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in Dehong prefecture, Yunnan province, China.
    05/2014; 35(5):528-32.
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    ABSTRACT: Although many severe acute respiratory syndrome-like coronaviruses (SARS-like CoVs) have been identified in bats in China, Europe and Africa, most have a genetic organization significantly distinct from human/civet SARS CoVs in the receptor-binding domain (RBD), which mediates receptor binding and determines the host spectrum, resulting in their failure to cause human infections and making them unlikely progenitors of human/civet SARS CoV. Here, a viral metagenomic analysis of 268 bat rectal swabs collected from four counties in Yunnan province has identified hundreds of sequences relating to alpha- and betacoronaviruses. Phylogenetic analysis based on a conserved region of the RNA-dependent RNA polymerase gene revealed that alphacoronaviruses had diversities with some obvious differences from those reported previously. Full genomic analysis of a new SARS-like CoV from Baoshan (LYRa11) showed that it was 29,805 nt in length with 13 open reading frames (ORFs), sharing 91% nt identity with human/civet SARS CoVs and the most recently reported SARS-like CoV Rs3367, while 89% with other bat SARS-like CoVs. Notably it showed highest sequence identity with the S gene of SARS CoVs and Rs3367, especially in the RBD region. Antigenic analysis showed that the S1 domain of LYRa11 could be efficiently recognized by SARS-convalescent human serum indicating that LYRa11 is a novel virus antigenically close to SARS CoV. Recombination analyses indicate that LYRa11 is likely a recombinant descended from parental lineages that had evolved into a number of bat SARS-like CoVs. Although many SARS-like coronaviruses (CoV) have been discovered in bats worldwide, there are significant different genic structure, particularly in S1 domain, which is responsible for host tropism determination, between bat SARS-like CoVs and human SARS CoVs, indicating that most reported bat SARS-like CoVs are not the progenitors of human SARS CoV. We have identified diverse alphacoronaviruses and a close relative (LYRa11) to SARS CoV in bats collected in Yunnan, China. Further analysis showed that alpha- and betacoronaviruses have different circulation and transmission dynamics in bat populations. Notably full genomic sequence and antigenic study demonstrated that LYRa11 is phylogenetically and antigenically closely related to SARS CoV. Recombination analyses indicate that LYRa11 is a recombinant from certain bat SARS-like CoVs circulating in Yunnan province.
    Journal of Virology 04/2014; · 5.08 Impact Factor
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    ABSTRACT: Bats are considered important animal reservoirs for many viruses pathogenic to humans. A viral metagenomic based approach was used to study gut specimens of 78 insectivorous bats in Yunnan province, China. Seventy-four reads were found to be related to group A rotavirus (RVA). Further RT-PCR screening and viral isolation on cell cultures confirmed the presence of a novel RVA strain, named RVA/Bat-tc/MSLH14/2012/G3P[3], in one of 16 lesser horseshoe bats (6%). Full genomic sequencing analyses showed that MSLH14 possessed the following genotype constellation: G3-P[3]-I8-R3-C3-M3-A9-N3-T3-E3-H6, which is akin to human and animal rotaviruses believed to be of feline/canine origin. Phylogenetic analysis indicated that VP7 was most closely related to bovine RVA strains from India, whereas VP4 was most closely related to an unusual human RVA strain CMH222 with animal characteristics isolated in Thailand. The remaining gene segments were only distantly related to a range of animal RVA strains, most of which are believed to be related to feline/canine RVAs. Experimental infection showed that bat RVA strain MSLH14 was highly pathogenic to suckling mice, causing 100% mortality when inoculated orally with a titer as low as 5×10(2) TCID50 virus. As this virus is not closely related to any known RVA strain it is tempting to speculate that this is a true bat RVA strain rather than an interspecies transmitted virus. However, further screening of bat populations, preferentially juvenile animals, will be crucial to determine whether or not this virus is widely distributed into the bat population.
    Journal of Virology 09/2013; · 5.08 Impact Factor
  • Emerging Infectious Diseases 04/2013; 19(4):672-3. · 6.79 Impact Factor
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    ABSTRACT: A total of 54,673 mosquitoes were collected at 11 sites located near the China-Myanmar border in the western part of Yunnan Province during July and August 2007. There were 29 species in 4 genera identified from the collections, including 12 species of Culex, 12 species of Anopheles, 3 species of Aedes, and 2 species of Armigeres. Culex tritaeniorhynchus Giles (67.9%, 37,119/54,673) and Anopheles sinensis Wiedemann (25.9%, 14,170/54,673) were the most abundant species in this investigation. Virus was isolated using BHK-21 and C6/36 cells from 22 of 510 mosquito pools. Isolates included Japanese encephalitis virus (JEV) and Getah virus (GETV), which were identified by serological and molecular methods. Twenty JEV strains were isolated from Cx. tritaeniorhynchus (15 isolates), An. sinensis (3 isolates), and Armigeres subalbatus Coquillett (2 isolates), and 2 GETV strains were isolated from Culex pseudovishnui Colless and Cx. tritaeniorhynchus. This study suggests that Ar. subalbatus is a potentially important local vector because of the high JEV infection ratio found in this species. Enzootic JEV transmission persists in this area and therefore, surveillance for human disease caused by JEV and GETV should be conducted in the region.
    Japanese journal of infectious diseases. 05/2012; 65(3):215-21.
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    ABSTRACT: Economic development and increased tourism in the southern region of Yunnan Province in China, adjacent to several countries in Southeast Asia, has increased the likelihood of import and export of vectors and vector-borne diseases. We report the results of surveillance of mosquitoes and mosquito-borne arboviruses along the border of China-Myanmar-Laos in 2005 and 2006, and information associating several arboviruses with infections and possibly disease in local human populations. Seventeen mosquito species representing four genera were obtained, and 14 strains of mosquito-borne viruses representing six viruses in five genera were isolated from Culex tritaeniorhynchus. In addition, IgM against Japanese encephalitis virus, Sindbis virus, Yunnan orbivirus and novel Banna virus was detected in acute-phase serum samples obtained from hospitalized patients with fever and encephalitis near the areas where the viruses were isolated. This investigation suggests that Japanese encephalitis virus, Sindbis virus, and lesser-known arboviruses circulate and may be infecting humans in the China-Myanmar-Laos border region.
    The American journal of tropical medicine and hygiene 05/2011; 84(5):738-46. · 2.53 Impact Factor
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    ABSTRACT: An outbreak of hemorrhagic fever with renal syndrome occurred among students in a college (College A) in Kunming, Yunnan province, China in 2003. Subsequent investigations revealed the presence of hantavirus antibodies and antigens in laboratory rats at College A and two other institutions. Hantavirus antibodies were detected in 15 additional individuals other than the index case in these three locations. Epidemiologic data indicated that the human infections were a result of zoonotic transmission of the virus from laboratory rats. A virus was isolated from rats in College A and the full-length genome sequence revealed that this was a new Hantaan virus isolate, designated strain KY. Sequence analysis of the three genome segments indicated that this new isolate is a reassortant derived from human and rat Hantaan viruses. Further sequence analysis of the medium (M) genome segment revealed that it originated from a recombination event between two rat Hantaan virus lineages.
    Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases 04/2010; 10(5):638-44. · 3.22 Impact Factor
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    ABSTRACT: Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines.
    Virology Journal 01/2010; 7:345. · 2.09 Impact Factor
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    ABSTRACT: We recently determined that Nanjianyin virus, isolated from serum of a patient in Yunnan Province, China, in 1989, is a type of Kyasanur Forest disease virus. Results of a 1987-1990 seroepidemiologic investigation in Yunnan Province had shown that residents of the Hengduan Mountain region had been infected with Nanjianyin virus.
    Emerging Infectious Diseases 03/2009; 15(2):326-8. · 6.79 Impact Factor

Publication Stats

45 Citations
31.59 Total Impact Points


  • 2013
    • Yunnan Center for Disease Control and Prevention
      Yün-nan, Yunnan, China
  • 2012
    • Chinese Center For Disease Control And Prevention
      • Institute for Viral Disease Control and Prevention
      Beijing, Beijing Shi, China
  • 2009
    • Beijing Centers for Disease Control and Prevention
      Peping, Beijing, China