Y.‐F. Lan

Shandong Agricultural University, T’ai-an-shih, Shandong Sheng, China

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Publications (3)2.76 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Cucumber mosaic virus (CMV) isolates are currently divided into two main groups, I and II according to their genomic sequences. The group I is further divided into two subgroups IA and IB. We performed a phylogenetic analysis of the genome regions containing 1a, 2a, 2b, coat protein (CP), and movement protein (MP) genes of 5 CMV isolates from China and other 28 CMV isolates available in the GenBank. The results indicated that CMV isolates could be genetically divided into three groups I, II, and III according to the genes encoding MP, CP, 1a, and 2a proteins and to the 2 groups according to the gene 2b. Group I could be further divided into two subgroups (IA and IB) according to the genes encoding CP, MP, 2a, and 2b proteins and to the three subgroups (IA, IB, and IC) according to the gene encoding 1a protein. Four of 5 examined Chinese CMV isolates belonged to the subgroup IB, while the remaining isolate was a natural inter-subgroup reassortant. We found that the 2b gene of CMV was under positive selection, while the other genes were under negative selection. No evidence of the selection associated with a host adaptation or geographic distribution was found.
    Acta virologica 02/2009; 53(2):89-97. · 0.76 Impact Factor
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    ABSTRACT: The complete genomic sequence of a Chinese Potato virus X isolate FX21 (PVX-FX21) was determined from three overlapping cDNA clones. The genome of PVX-FX21 is 6435 nucleotides in length excluding the poly(A) tail and contains five open reading frames (ORFs). Its entire genomic sequence shares 95.2–96.3% identities with Asian and European isolates, and 77.3–77.8% with American isolates. Phylogenetic analysis of the complete genomic sequence reveals two groups: the Eurasian group and the American group. PVX-FX21 belongs to the Eurasian group and forms a separate sub-branch with three Asian isolates. Similar analyses of the coat protein genes of 37 PVX isolates also reveal two major groups. All PVX isolates from Asia are clustered to group I, whereas isolates from Europe and America are clustered to both groups. Nucleotide sequence diversity analyses show that there is no geographical differentiation between PVX isolates and that constraint on the ORF encoding RNA-dependent RNA polymerase is much higher than those on the other four ORFs.
    Journal of Phytopathology 02/2008; 156(6):346 - 351. · 1.00 Impact Factor
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    ABSTRACT: In 2005, rose plants (Rosa rugosa cv. ‘Plena’) exhibiting typical phytoplasma disease symptoms of stunting, yellowing, witches’-broom and dieback were observed in Pingyin, Shandong Province, China. The disease, rose witches’-broom (RoWB), is progressively destructive and can be graft-transmitted. Polymerase chain reaction (PCR), sequencing of PCR products and electron microscopy were used to investigate the possible association of phytoplasma with RoWB. All results indicated that presence of phytoplasma in the symptomatic rose plants. Sequence alignment of 16S rRNA gene, tuf gene and rp gene confirmed that the phytoplasma associated with RoWB is the causal agent of Paulownia witches’-broom disease, which might be transmitted from the paulownia tree that is several meters away. To our knowledge, this is the first report of the molecular characterization of phytoplasma infecting rose in China.
    Journal of Phytopathology 11/2007; 156(2):93 - 98. · 1.00 Impact Factor

Publication Stats

8 Citations
2.76 Total Impact Points

Institutions

  • 2007–2008
    • Shandong Agricultural University
      • Department of Plant Pathology
      T’ai-an-shih, Shandong Sheng, China