Bhaswati Ganguli

Publications (4)5.38 Total impact

• Source

  Available from: academicjournals.org

  Article: GST polymorphic status modifying the arsenic induced clinical manifestations in people of the southern part of West Bengal, India

  Sarmishtha Chanda, Bhaswati Ganguli, Uma B Dasgupta, Debendranath Guha Mazumder
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  ABSTRACT: Persons chronically exposed to environmental arsenic through their drinking water experience various arsenic induced clinical manifestations which includes keratosis and pigmentary changes in the skin. However the response varies widely among persons. To study whether Glutathione-S-Transferase (GST) gene polymorphism plays any role in this variation, a total of 78 study subjects were recruited from the villages of southern region of West Bengal, India. Concentration of arsenic in their urine and drinking water were determined by Atomic Absorption Spectrophotometry Hydride Generation (AAS) system. Extent of clinical manifestations in the form of pigmentation and keratosis were determined by evaluation of the severity and giving clinical symptom score according to their degree of severity. The individual's GST status was determined by multiplex PCR approach. The persons having skin manifestation given a score called clinical symptom score by which the degree of the clinical manifestation was determined. Results showed that genetic polymorphism of GSTM1 and T1 were significantly associated with urinary arsenic and clinical manifestation in higher exposure group. Persons having null genotype have significantly decreased urinary arsenic (p< 0.01) and increased clinical symptom (p<0.05) score relative to persons with GSTM1 or GST T1 non-null genotype of same arsenic exposure group. The study signifies that GST status determines the extent of biotransformation of arsenic in the body which further determines the degree of arsenic induced clinical manifestations in exposed persons.
  Journal of Toxicology and Environmental Health Sciences. 07/2011; 3:171-175.
• Article: Comparison of drinking water, raw rice and cooking of rice as arsenic exposure routes in three contrasting areas of West Bengal, India.

  Debapriya Mondal, Mayukh Banerjee, Manjari Kundu, Nilanjana Banerjee, Udayan Bhattacharya, Ashok K Giri, Bhaswati Ganguli, Sugata Sen Roy, David A Polya
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  ABSTRACT: Remediation aimed at reducing human exposure to groundwater arsenic in West Bengal, one of the regions most impacted by this environmental hazard, are currently largely focussed on reducing arsenic in drinking water. Rice and cooking of rice, however, have also been identified as important or potentially important exposure routes. Quantifying the relative importance of these exposure routes is critically required to inform the prioritisation and selection of remediation strategies. The aim of our study, therefore, was to determine the relative contributions of drinking water, rice and cooking of rice to human exposure in three contrasting areas of West Bengal with different overall levels of exposure to arsenic, viz. high (Bhawangola-I Block, Murshidibad District), moderate (Chakdha Block, Nadia District) and low (Khejuri-I Block, Midnapur District). Arsenic exposure from water was highly variable, median exposures being 0.02 μg/kg/d (Midnapur), 0.77 μg/kg/d (Nadia) and 2.03 μg/kg/d (Murshidabad). In contrast arsenic exposure from cooked rice was relatively uniform, with median exposures being 0.30 μg/kg/d (Midnapur), 0.50 μg/kg/d (Nadia) and 0.84 μg/kg/d (Murshidabad). Cooking rice typically resulted in arsenic exposures of lower magnitude, indeed in Midnapur, median exposure from cooking was slightly negative. Water was the dominant route of exposure in Murshidabad, both water and rice were major exposure routes in Nadia, whereas rice was the dominant exposure route in Midnapur. Notwithstanding the differences in balance of exposure routes, median excess lifetime cancer risk for all the blocks were found to exceed the USEPA regulatory threshold target cancer risk level of 10(-4)-10(-6). The difference in balance of exposure routes indicate a difference in balance of remediation approaches in the three districts.
  Environmental Geochemistry and Health 12/2010; 32(6):463-77. · 1.62 Impact Factor
• Article: Arsenic exposure induces genomic hypermethylation.

  Sunipa Majumdar, Sarmishtha Chanda, Bhaswati Ganguli, D N Guha Mazumder, Sarbari Lahiri, Uma B Dasgupta
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  ABSTRACT: Gene-specific hypermethylation has previously been detected in Arsenic exposed persons. To monitor the level of whole genome methylation in persons exposed to different levels of Arsenic via drinking water, DNA was extracted from peripheral blood mononuclear cells of 64 persons. Uptake of methyl group from (3)H labeled S-Adenosyl Methionine after incubation of DNA with SssI methylase was measured. Results showed statistically significant (P = 0.0004) decrease in uptake of (3)H methyl group in the persons exposed to 250-500 microg/L arsenic, indicating genomic hypermethylation.
  Environmental Toxicology 06/2009; 25(3):315-8. · 2.41 Impact Factor
• Article: Methylation status of promoter-associated CpG islands in primary acute myeloid leukemia.

  Bama Charan Mondal, Ashis Mukhopadhyay, Utpal Chaudhuri, Bhaswati Ganguli, Uma B Dasgupta
  Acta Haematologica 02/2009; 120(4):207-10. · 1.35 Impact Factor