Hideya Yoshida

Okayama University, Okayama, Okayama, Japan

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Publications (17)19.3 Total impact

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    ABSTRACT: To examine the effects of pesticides on insect biodiversity, a population survey was conducted in conventionally managed, low-input, and organic peach orchards. Pitfall traps were used to sample a total of 6489 insects representing 151 species at seven study sites. Results of a population survey suggest that pesticide application adversely affected insect biodiversity in peach orchards and that the magnitude of the adverse effect might be greater for herbicide application than for insecticide application. The usefulness of carabids and ants as biological indicators of the effects of pesticides was also evaluated. Results suggest that the ant species Tetramorium tsushimae is a good indicator in peach orchards. In contrast, carabids are not suitable indicators. KeywordsAnt–Biodiversity–Biological indicator–Carabid–Population survey
    Applied Entomology and Zoology 08/2011; 46(3):335-342. DOI:10.1007/s13355-011-0041-2 · 0.88 Impact Factor
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    ABSTRACT: In this study, we attempted a population survey of spiders in peach orchards with and without pesticide application; however, we had difficulty discriminating them. To identify spiders, we determined the nucleotide sequences of a ca. 640-bp region in the mitochondrial cytochrome oxidase subunit I (COI) gene from 17 species belonging to eight families. To identify five wolf spiders (Araneae: Lycosidae), which are the most abundant spiders in peach orchards in Okayama Prefecture, PCR and restriction fragment length polymorphism were developed on the basis of the COI gene sequences. Population survey results suggest that pesticide applications adversely affect spider populations in peach orchards.
    Applied Entomology and Zoology 01/2011; 46(1):81-86. DOI:10.1007/s13355-010-0011-0 · 0.88 Impact Factor
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    ABSTRACT: Abstract A proportion of Helicoverpa armigera collected from fields in Okayama Prefecture (Western Japan; 34.6°N, 134.1°E) does not enter diapause when reared under a short days at 20 °C during the larval stages. However, diapause in such photo-insensitive individuals can be induced when they are reared at moderately low temperatures, such as 15 °C, regardless of photoperiod. To determine whether such photo-insensitive individuals can survive overwintering in fields, the present study compares the cold hardiness and sugar content between nondiapausing and diapausing pupae of photo-insensitive individuals selected over several generations at 20 °C under a short day photoperiod (LD 10 : 14 h). Diapausing and nondiapausing pupae are obtained under the short days by rearing at 15 and 20 °C, respectively, during larval and pupal stages. These pupae are stepwise acclimated at a reduction of 5 °C every 5 days to 0 °C. Maximum survival periods of nondiapausing and diapausing pupae at 0 °C are approximately 30 and 90 days, respectively. Trehalose content in diapausing pupae increases, reaches a maximum level (1.95 mg 100 mg−1 in males and 2.1 mg 100 mg−1 in females) 28 days after exposure to 0 °C and then decreases. On the other hand, glucose content in diapausing pupae increases (maximum level: 0.32 mg 100 mg−1 in males and 0.21 mg 100 mg−1 in females) with decreasing trehalose content 42 days after exposure to 0°C. The decrease in trehalose content and the increase in glucose content may be linked to termination of diapause in H. armigera. These results suggest that, in Japan, the photo-insensitive individuals can only survive in the mild winters of southern regions, and not in the severe winters of northern regions.
    Physiological Entomology 08/2008; 33(3):251 - 256. DOI:10.1111/j.1365-3032.2008.00630.x · 1.43 Impact Factor
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    ABSTRACT: The olfactory responses of adult males and females of the fruit-piercing moth, Oraesia excavata, to lactones as specific components among ripe peach fruit odors were recorded by electroantennogram (EAG) techniques and trap captures in the field. Six lactones (gamma-hexalactone, gamma-octalactone, delta-octalactone, gamma-decalactone, delta-decalactone and gamma-dodecalactone) and a green leaf volatile compound (cis-3-hexen-1-ol) as the reference compound for normalization were used to measure EAG responses. The EAG response to gamma-hexalactone, shown to be the highest among the six lactones tested, did not reach as high as that to a mixture of five lactones when 10% concentrations (v/v) of the lactones were used. There was no significant difference between males and females in EAG responses to those compounds. In the field experiment, the number of moths captured by traps baited with a mixture of the five lactones (gamma-hexalactone, gamma-octalactone, gamma-decalactone, delta-decalactone and gamma-dodecalactone = 142: 7: 145 : 70: 28, v/v) was about half that captured with ripe peach fruit; however, the moths were not captured by traps with individual lactones. These results show that O. excavata is attracted by a mixture of lactones, but not by individual lactones, although individual lactones are recognized by antennal receptors.
    Applied Entomology and Zoology 05/2008; 43(2):265-269. DOI:10.1303/aez.2008.265 · 0.88 Impact Factor
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    ABSTRACT: Fruit-piercing moths such as Oraesia excavata (Butler), O. emarginata (Fabricius) and Adris tyrannus amurensis (Staudinger) are attracted to the volatiles emitted from various fruits and cause damage to these fruits. Especially, the volatiles emitted from ripe peach fruits are known to be attractive to the moths. In the present experiment, the repellency of a volatile compound, sec-butyl beta-styryl ketone (4-methyl-l-phenylhex-l-en-3-one), was examined in or around peach orchards in order to prevent damage by fruit-piercing moths. In orchard experiments the moths were attracted and captured by ripe peach fruit traps. The number of captured moths in the traps was reduced by sec-butyl beta-styryl ketone treatment, and hardly any moths were captured at a release rate of 1.0 mg/day. A significant reduction in ripe peach fruit damage was recorded with the use of sec-butyl beta-styryl ketone (10 tubes of 1.0 mg/day release rate/tree). These results suggest that sec-butyl beta-styryl ketone is a good repellent against fruit-piercing moths in the peach orchards.
    Applied Entomology and Zoology 08/2007; 42(3):433-437. DOI:10.1303/aez.2007.433 · 0.88 Impact Factor
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    ABSTRACT: The cold hardiness in adult males and females of Frankliniella occidentalis (Pergande) reared at different conditions were investigated. Supercooling points (SCPs) were about -26 degrees C in both males and females. The SCPs were not affected by the rearing conditions of photoperiod and temperature. However, the survival periods of males and females at low temperatures from -15 degrees C to 5 degrees C were predominantly affected by rearing temperatures. Males and females reared at 15 degrees C survived longer at the low temperatures than those reared at 20 degrees C. Females survived for much longer than males at the low temperatures except -15 degrees C. Furthermore, the survival periods of males and females at the low temperatures were affected by water and food applications. However, the effects were smaller in both males and females at 0 degrees C from -5 degrees C for 12 h to + 5 degrees C for 12 h than 5 degrees C, especially less water effect was observed in males at 0 degrees C. Males and females given food maximally survived for about 30d and 40d at 0 degrees C, and about 40 d and 60 d at 5 degrees C, respectively. These results show that F occidentalis can overwinter only in relative mild winter or artificial conditions.
    Applied Entomology and Zoology 05/2007; 42(2):223-229. DOI:10.1303/aez.2007.223 · 0.88 Impact Factor
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    ABSTRACT: A storage protein gene, CsSP2, was cloned and sequenced from the rice stem borer, Chilo suppressalis. Analysis of the cDNA sequence revealed an open reading frame of 2,118 bp in length which encodes an arylphorin-like protein with a calculated molecular weight of 83.7 kDa. The expression level of CsSP2 was higher in non-diapausing larvae than in diapausing larvae at 20 degrees C. When diapausing larvae were acclimatized in a stepwise fashion from 20 degrees C to 5 degrees C, CsSP2 expression was up-regulated five days after acclimation at 15 degrees C. The level was maintained for 10 days after acclimation at 5 degrees C and down-regulated thereafter. Some up-regulation in CsSP2 expression was detected in the post-diapause state. In non-diapausing larvae, CsSP2 expression was down-regulated in the course of cold acclimation. Protein products of CsSP2 might have an important role as an amino acid reservoir or a cellular defense mechanism during diapause.
    Applied Entomology and Zoology 02/2007; 42(1):35-40. DOI:10.1303/aez.2007.35 · 0.88 Impact Factor
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    ABSTRACT: The complete cDNA sequences of heat shock protein 90 (hsp90) and of heat shock cognate protein 70 (hsc70) were cloned by reverse transcription polymerase chain reaction from the rice stem borer, Chilo suppressalis Walker. They potentially encode a 717-amino-acids (hsp90) and a 652-amino-acids (hsc70) protein, with calculated molecular weight of 82.5 and 71.3 kDa, respectively. The deduced amino acid sequence of hsp90 showed the highest homology of 97.2% to Spodoptera frugiperda hsp90. The closest match of C. suppressalis hsc70 was with Manduca sexta hsc70 at 98.0% identity. Expression of hsp90 in diapausing larvae was higher than that in non-diapausing larvae. No such up-regulation in diapausing larvae was observed for hsc70. In non-diapausing larvae, but not in diapausing ones, hsp90 expression was up-regulated by cold acclimation. Hsc70 expression slightly decreased during cold acclimation irrespective of the state of diapause. Involvement of hsp90 and hsc70 in larval diapause and cold tolerance acquisition in C. suppressalis is discussed.
    Archives of Insect Biochemistry and Physiology 09/2006; 63(1):36-47. DOI:10.1002/arch.20138 · 1.16 Impact Factor
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    ABSTRACT: Overwintering larvae of the rice stem borer, Chilo suppressalis accumulate glycerol and are freezing tolerant to about -25 degrees C. However, non-diapausing larvae cannot accumulate glycerol and are killed by freezing. We compared the extent of tissue damage, the effects of glycerol concentration, and the transport of glycerol and water in fat body tissues from these larvae at selected freezing temperatures. Tissues from overwintering larvae, but not non-diapausing larvae, survive when frozen at -20 degrees C with 0.25 M glycerol, but the protection afforded by glycerol is offset by the water-channel inhibitor mercuric chloride. Glycerol in higher concentration (0.75 M) affords some protection even to the fat body of non-diapausing larvae. Radiotracer assays of overwintering larvae show that water leaves the tissues during freezing while glycerol enters, and that mercuric chloride disrupts this process. Transport is also disrupted after lethal freezing at -35 degrees C. Therefore, membrane transport of water and glycerol is involved in the avoidance of freezing injury to fat body cells of the rice stem borer, apparently by mediating the replacement of water with glycerol in freezing-tolerant tissues.
    Journal of Insect Physiology 03/2006; 52(2):215-20. DOI:10.1016/j.jinsphys.2005.11.001 · 2.50 Impact Factor
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    ABSTRACT: Helicoverpa armigera exhibits a facultative pupal diapause, which depends on temperature and photoperiod. Pupal diapause is induced at 20 degrees C by short photoperiods and inhibited by long photoperiods during the larval stage. Sensitive stages for photoperiodic induction of pupal diapause were determined by transferring larvae of various instars between long and short photoperiods. When larvae reared at 20 degrees C under a short photoperiod during egg to fourth instar were transferred to a long photoperiod at the early fifth instar, diapause was not induced. However, when larvae were transferred to a long photoperiod in the middle of fifth instar, diapause was induced. On the other hand, when larvae reared under a long photoperiod during egg to fourth instar were transferred to a short photoperiod at the early fifth instar, about 80 to 100% pupae entered diapause. These results show that the early fifth larval instar is the main sensitive stage for pupal diapause determination even though photoperiod has some effect in the third and fourth instars.
    Applied Entomology and Zoology 08/2005; 40(3):457-460. DOI:10.1303/aez.2005.457 · 0.88 Impact Factor
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    ABSTRACT:   Even though overwintering larvae of the rice stem borer, Chilo suppressalis, are freeze-tolerant, they cannot survive below −30 °C. Furthermore, nondiapausing larvae cannot survive freezing. However, the cause of death due to freezing is unclear. To identify the cause of death by freezing in larvae, those tissues most injured by low temperatures are identified using the vital stain trypan blue. In overwintering larvae, the midgut of dead larvae stains blue, and remarkable colour density differences between dead and surviving larvae are observed in the midgut. In nondiapausing larvae incubated at −10 °C for several hours, the fat body of dead larvae is strongly stained. Furthermore, increases in mortality with treatment time correspond with increases in the area of the fat body stained. Sterile nondiapausing larvae with lower supercooling points, below −20 °C, do not freeze at −10 °C and survive the treatment. However, all the larvae die when subjected to inoculative freezing at −10 °C, and the fat body stains blue. These results suggest that the midgut in overwintering larvae and the fat body in nondiapausing larvae have the lowest tolerance to freezing.
    Physiological Entomology 06/2005; 30(4):324 - 331. DOI:10.1111/j.1365-3032.2005.00466.x · 1.43 Impact Factor
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    ABSTRACT: To understand overwintering of the cotton boll worm Helicoverpa armigera, cold hardiness and sugar content are compared between diapausing and nondiapausing pupae. Diapausing and nondiapausing pupae reared at 20 °C under short and long photoperiods are acclimatized with a reduction of 5 °C per 5 days to 0 °C. When the acclimation temperature reaches 0 °C, the survival of diapausing pupae is assessed. The survival gradually decreases as the period of treatment progresses and approximately half survive for 112 days. However, nondiapausing pupae survive only 14 days after exposure to 0 °C. The surpercooling points of nondiapausing, diapausing and acclimatized pupae are approximately −17 °C. The major sugars contained in pupae are trehalose and glucose. Even though trehalose contents in diapausing pupae (initial level: 0.6 mg 100 mg−1 fresh weight) increase significantly during cold acclimation and continue increasing until 58 days after exposure to 0 °C (maximum level: 1.8 mg 100 mg−1), glucose is maintained at low levels (0.02 mg 100 mg−1) for 56 days at 0 °C. However, glucose contents increase (maximum level: 0.8 mg 100 mg−1) with decreasing contents of trehalose 84 days after exposure to 0 °C. Glycogen content gradually decreases during cold acclimation. When nondiapausing pupae are acclimatized with a reduction of 5 °C per 5 days to 5 °C from the beginning of pupation until the eyespots move, trehalose content increases (maximum level: 1.0 mg 100 mg−1). Glucose contents in nondiapausing pupae increase before eclosion (0.09 mg 100 mg−1). From these results, diapausing pupae of H. armigera can overwinter in regions where average winter temperatures are higher than 0 °C, but nondiapausing pupae cannot.
    Physiological Entomology 02/2005; 30(1):36 - 41. DOI:10.1111/j.0307-6962.2005.00422.x · 1.43 Impact Factor
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    ABSTRACT: Chilo suppressalis transferrin cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). The clone (2,248 bp) encodes a protein of 644 amino acids with a calculated molecular weight of 71,805 Da. The deduced amino acid sequence showed significant homology with known insect transferrins from Bombyx mori (82%), Manduca sexta (77%), Blaberus discoidalis (43%), Riptortus clavatus (42%), Aedes aegypti (36%), Sarcophaga peregrina (32%), Drosophila melanogaster (33%) and D. silvestris (32%). Transferrin expression was examined by RNA gel blot analysis using diapausing and non-diapausing larvae of C. suppressalis. As a result, higher levels of expression were observed in diapausing larvae.
    Applied Entomology and Zoology 08/2004; 39(3):463-468. DOI:10.1303/aez.2004.463 · 0.88 Impact Factor
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    Applied Entomology and Zoology 01/2003; 38(1):65-68. DOI:10.1303/aez.2003.65 · 0.88 Impact Factor
  • Applied Entomology and Zoology 01/2002; 37(3):341-346. DOI:10.1303/aez.2002.341 · 0.88 Impact Factor
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    ABSTRACT: Diapause induction and termination were compared among three different geographic populations of Helicoverpa armigera from Ishigaki (24.3 degrees N; 124.2 degrees E), Okayama (Ushimado, 34.6 degrees N; 134.1 degrees E) and Kanazawa (36.6 degrees N; 136.7 degrees E) in Japan. The critical daylength for pupal diapause induction among the three populations was between 12L:12D and 14L:10D in the larval stage. No clinal latitudinal variation in the critical daylength was found among the three populations. Most of the diapausing pupae emerged when they were continuously maintained at 20 degrees C under 12L:12D, suggesting that cold exposure (chilling) is not necessary to break the pupal diapause. Furthermore, the time required for diapause termination of the Okayama population was the longest (214 days), followed by the Ishigaki (149 days) and Kanazawa (73 days) populations at 20 degrees C (without chilling) under the 12L:12D photoperiod. These results showed no clinal latitudinal variation in diapause termination.
    Applied Entomology and Zoology 08/2000; 35(3):357-360. DOI:10.1303/aez.2000.357 · 0.88 Impact Factor
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    ABSTRACT: The inheritance of gramine indole alkaloid was studied in F1 and F2 generations derived from crosses between wild ( Hordeum The inheritance of gramine indole alkaloid was studied in F1 and F2 generations derived from crosses between wild ( Hordeum vulgare subsp. spontaneum) and cultivated ( H. vulgare subsp. vulgare) barley at adult stage in the field. The means of gramine vulgare subsp. spontaneum) and cultivated ( H. vulgare subsp. vulgare) barley at adult stage in the field. The means of gramine content were very low in F1s and F2s in April and May. The ratio of progeny containing gramine to those without gramine (near content were very low in F1s and F2s in April and May. The ratio of progeny containing gramine to those without gramine (near zero or undetectable) fit a mono-genic or di-genic model in F2 generation. However, the content of gramine might be controlled zero or undetectable) fit a mono-genic or di-genic model in F2 generation. However, the content of gramine might be controlled by minor genes in the gramine-containing plants. The direction of dominance was toward lower gramine content. The averages by minor genes in the gramine-containing plants. The direction of dominance was toward lower gramine content. The averages of dominance ( h/d) in F1 and F2 ranged from partial to complete. Broad-sense heritabilities were high (0.70 to 0.77). The of dominance ( h/d) in F1 and F2 ranged from partial to complete. Broad-sense heritabilities were high (0.70 to 0.77). The usefulness of hybrids for breeding aphid resistance and possibility increasing palatability of grazing pasture for sheep and usefulness of hybrids for breeding aphid resistance and possibility increasing palatability of grazing pasture for sheep and cattle is discussed. cattle is discussed.
    Euphytica 01/1999; 106(2):181-185. DOI:10.1023/A:1003535823329 · 1.69 Impact Factor