[Show abstract][Hide abstract] ABSTRACT: Objective: It was aimed to determine the expression profiles of miRNAs in patients with diffuse and anaplastic brain tumors. Methods: miRNA expression profiles of 50 cases (19 Female, 31 Male) diagnosed with brain tumor [32 Glioblastoma (GBM), 10 diffuse astrocytoma (DA), 8 anaplastic oligodendroglioma (AO)] in Ege University, Department of Neurosurgery and of brain tumor cell lines (U-87 MG, U-118 MG and LN18) studied by the microarray method. Results: In all cases miR-21 expression level was high and no miR-124 expression was detected. In GBM, miR-495 and miR-432 expression showed significant 2-fold increase, and miR-708-3p, mir-339-5p and miR-4286 expressions 4-fold, mir-331-3p, miR-625-3p, and miR-20a-3p showed 5-fold reduction compared to cell lines. miRNA expressions compared GBM and AO, it was detected that miR-34c-3p, miR-132-5p, mir-605, miR-3130-3p, miR-3127, mir-517a-pre, mir-548b-3p, miR-921, miR-769-5p were significantly increased. mir-204 expression was significantly decreased in AO compared to cell lines. In DA, miRNA expression was significantly increased in 18 and decreased in 7. There was no significant miRNA expression changes in AO compared with DA. miRNA expressions of DA, AO and GBM cases were compared and significant alterations were found in 29 miRNA expressions. Conclusion: miRNA expression profiles of GBM were significantly different from AO. It was emphasized that, new genes could be effective in tumor development supposed to be regulated by miRNAs. New specific gene targets regulated by miRNA expression could be predicted good clinical outcome in brain tumors.
Journal of Neurological Sciences 01/2015; 32(43):64-88. · 0.11 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Collision tumors in the paranasal region are extremely rare with limited literature data. To the best of our knowledge, this is the first report of associations of squamous cell carcinoma-esthesioneuroblastoma and lymphoma-hemangiopericytoma in the paranasal region. Preoperatively, radiological and clinical findings should be evaluated carefully for the diagnosis and two or more biopsy specimens should be taken from different morphological parts of the lesions. Adjuvant therapy should be planned according to two different histologies and special importance should be given to the tumor which indicates the prognosis of the patient. A multidisciplinary approach is required for the management of synchronous malignancies.
Kulak burun bogaz ihtisas dergisi: KBB = Journal of ear, nose, and throat 03/2014; 24(2):105-9.
[Show abstract][Hide abstract] ABSTRACT: Both leukocytoclastic vasculitis and metastatic pulmonary calcification are conditions that rarely occur during the course of multiple myeloma. We present a multiple myeloma patient that had severe dyspnea due to metastatic pulmonary calcinosis, and ulceronecrotic skin lesions caused by leukocytoclastic vasculitis. After 3 courses of standarddose chemotherapy all skin and pulmonary lesions disappeared. Autologous peripheral stem cell transplantation was performed and during 1 year of follow-up the patient was in complete remission; after 1 year, laboratory test results indicated disease relapse. Although the patient was treated with bortezomib and dexamethasone, the disease progressed. Non-myeloablative allogeneic stem cell transplantation was performed, but despite of all treatment the patient died due to disease progression.
Conflict of interest:None declared.
[Show abstract][Hide abstract] ABSTRACT: The authors report 2 children with generalized cutaneous sclerosis exposed to pesticides containing malathion and diniconazole. Treatment with immunosuppressives resulted in partial improvement in the cutaneous signs, particularly over the face, trunk, and proximal limbs. The considerable exposure to chemicals related with the initiation of symptoms and absence of organ involvement suggested a diagnosis of chemically induced scleroderma-like disorder. Although autoantibodies were negative, previously reported relevant associations of anti-kinetochore and anti-topoisomerase function of active ingredients-diniconazole and phosphorodithioate-and solvents of these pesticides are also discussed. Careful follow-up for systemic involvement is warranted, since these agents may have triggered systemic scleroderma in these patients. Elimination of chemical exposure of children is stressed.
Archives of Environmental and Occupational Health 01/2012; 67(1):43-7. DOI:10.1080/19338244.2011.564231 · 0.93 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of the study is to the determine the profiles of tumor suppressor genes and oncogenes which cause brain tumor, establishing the association between the prognosis of cancer and the quantitation of genetic and epigenetic changes, and bringing a molecular approach to definite diagnosis. For this purpose, explant cell cultures are performed from the anaplastic brain tumor tissues of the cases. The expression analysis of the tumor suppressor genes (p53, RB1, PTEN, MGMT, RUNX3, DMBT1, PIKE) and oncogenes (EGFR, PIK3CA, MDM2, Olig2, GSTT1, COX-2 and hTERT) were determined by comparing the expression of GAPDH housekeeping gene using real-time online RT-PCR. The promoter regions of all the tumor suppressor genes' hypermethylation and also methylated and unmethylated copy numbers were determined with Q-PCR by using methylation specific primer and probes and the quantitation was carried out by comparing with each other. A significant difference was determined among the oncogenes; EGFR and hTERT gene expressions in patient tumor group. hTERT gene expression showed a significant difference with tumor grades. DMBT1 gene expression showed a significant difference with tumor grades. A prominent decrease was found in the aberration of tumor suppressor gene copy number in the glioma group. Gene copy number and gene expression of GSTT1 gene showed a significant correlation. RB1 and MGMT promoter methylation showed a significant difference in tumor patient group. Over expression of PIK3CA, EGFR and COX-2 among oncogenes and loss of copy number of PTEN, RB1 and RUNX3 among tumor suppressor genes found associated with short survival.
Journal of Neurological Sciences 12/2011; · 0.11 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Extraskeletal Ewing's sarcoma is a mesenchymal malign soft tissue tumor which generally affects the lower extremities and paravertebral region and is very rarely seen in the head and neck area. Early diagnosis and chemotherapy and radiotherapy program combines with surgical excision would be the appropriate treatment modality. In this article we present a case of extraskeletal Ewing's sarcoma which arise from the buccal area as an unusual localization together with information from the literature. In the physical examination of a 23-year old male patient who admitted to our clinic with a complaint of painless mass on his cheek which had been increasingly growing for approximately three months, a painless semi-mobile mass of approximately 5 x 3 cm in size with regular borders and elastic consistency was found within buccal soft tissue in the left maxillary area. The mass was excised under general anesthesia. No local recurrences or findings of distant metastases were observed during the 11-month postoperative follow-up.
Kulak burun bogaz ihtisas dergisi: KBB = Journal of ear, nose, and throat 01/2011; 21(4):225-8.