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M Shahid Mukhtar,
Anne-Ruxandra Carvunis,
Matija Dreze,
Petra Epple,
Jens Steinbrenner,
Jonathan Moore,
Murat Tasan, Mary Galli,
Tong Hao,
Marc T Nishimura, [......],
Huaming Chen,
Yijian He,
Jean Vandenhaute,
Frederick P Roth,
David E Hill,
Joseph R Ecker,
Marc Vidal,
Jim Beynon,
Pascal Braun,
Jeffery L Dangl
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ABSTRACT: Plants generate effective responses to infection by recognizing both conserved and variable pathogen-encoded molecules. Pathogens deploy virulence effector proteins into host cells, where they interact physically with host proteins to modulate defense. We generated an interaction network of plant-pathogen effectors from two pathogens spanning the eukaryote-eubacteria divergence, three classes of Arabidopsis immune system proteins, and ~8000 other Arabidopsis proteins. We noted convergence of effectors onto highly interconnected host proteins and indirect, rather than direct, connections between effectors and plant immune receptors. We demonstrated plant immune system functions for 15 of 17 tested host proteins that interact with effectors from both pathogens. Thus, pathogens from different kingdoms deploy independently evolved virulence proteins that interact with a limited set of highly connected cellular hubs to facilitate their diverse life-cycle strategies.
Science 07/2011; 333(6042):596-601. · 31.20 Impact Factor
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ABSTRACT: The YODA (YDA) mitogen-activated protein kinase pathway promotes elongation of the Arabidopsis zygote and development of its basal daughter cell into the extra-embryonic suspensor. Here, we show that the interleukin-1 receptor-associated kinase (IRAK)/Pelle-like kinase gene SHORT SUSPENSOR (SSP) regulates this pathway through a previously unknown parent-of-origin effect. SSP transcripts are produced in mature pollen but do not appear to be translated. Instead, they are delivered via the sperm cells to the zygote and the endosperm, where SSP protein transiently accumulates. Ectopic expression of SSP protein in the leaf epidermis is sufficient to activate YDA-dependent signaling. We propose that SSP protein produced from paternal transcripts upon fertilization triggers zygotic YDA activity, providing an essential temporal cue for the regulation of the asymmetric first division.
Science 04/2009; 323(5920):1485-8. · 31.20 Impact Factor
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Michael E Cusick,
Haiyuan Yu,
Alex Smolyar,
Kavitha Venkatesan,
Anne-Ruxandra Carvunis,
Nicolas Simonis,
Jean-François Rual,
Heather Borick,
Pascal Braun,
Matija Dreze,
Jean Vandenhaute, Mary Galli,
Junshi Yazaki,
David E Hill,
Joseph R Ecker,
Frederick P Roth,
Marc Vidal
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ABSTRACT: High-quality datasets are needed to understand how global and local properties of protein-protein interaction, or 'interactome', networks relate to biological mechanisms, and to guide research on individual proteins. In an evaluation of existing curation of protein interaction experiments reported in the literature, we found that curation can be error-prone and possibly of lower quality than commonly assumed.
Nature Methods 02/2009; 6(1):39-46. · 19.28 Impact Factor
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Michael E Cusick,
Haiyuan Yu,
Alex Smolyar,
Kavitha Venkatesan,
Anne-Ruxandra Carvunis,
Nicolas Simonis,
Jean-François Rual,
Heather Borick,
Pascal Braun,
Matija Dreze,
Jean Vandenhaute, Mary Galli,
Junshi Yazaki,
David E Hill,
Joseph R Ecker,
Frederick P Roth,
Marc Vidal
