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ABSTRACT: An aldehyde oxidase gene, designated as aldehyde oxidase2 (AhAO2), was cloned from Arachis hypogaea L. The gene contained a 4,050-bp open reading frame that encoded a putative protein of 1,350 amino acids. The deduced amino
acid sequence showed high identity with other plant AOs. The organ-specific expression pattern of AhAO2 has been examined, which indicates its dominant expression in leaves of peanut. The AhAO2 transcript level in leaves was greatly increased under exogenous ABA treatment for 1h. Over-expression of AhAO2 in Arabidopsis led to an increase in ABA level and enhanced drought tolerance after drought treatment. These results indicated the possible
involvement of AhAO2 in ABA synthesis and drought tolerance.
KeywordsCloning–Aldehyde oxidase–Ectopic expression–Drought tolerance–Abscisic acid–
Arachis hypogaea L.
Plant Molecular Biology Reporter 04/2012; 29(3):544-553. · 2.45 Impact Factor
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ABSTRACT: Ubiquitin (Ub)-conjugating enzymes (UBCs) are key enzymes involved in ubiquitination. Although UBCs have been shown to play important roles in regulating various aspects of plant growth and development, the role of plant UBCs in abiotic stress response needs to be examined further. Here we report the characterization of a ubiquitin-conjugating enzyme gene AhUBC2 from dehydrated peanut plants. The expression of AhUBC2 gene in peanut plants is responsive to physiological water-stress induced by polyethylene glycol (PEG6000), high salinity, abscisic acid (ABA) or low temperature. The constitutive expression of AhUBC2 gene in wild-type Arabidopsis confers improved tolerance to water-stress induced by sorbitol or soil drought in 35S::AhUBC2 transgenic plants. Constitutive expression of AhUBC2 results in significantly increased expressions of three stress-responsive genes P5CS1, RD29A and KIN1 in 35S::AhUBC2 Arabidopsis grown under normal conditions, whereas the expressions of other four stress-responsive genes NCED3, ABF3, RD29B and RD22 are not affected. The proline level in 35S::AhUBC2 Arabidopsis is significantly higher than that in wild-type Arabidopsis under both soil-drought stressed and control conditions. In contrast, there is no significant difference in the levels of NCED3 transcript and endogenous ABA between wild-type and 35S::AhUBC2 Arabidopsis. These results suggest that constitutive expression of AhUBC2 in Arabidopsis confers improved water-stress tolerance likely through activating an ABA-independent signaling pathway, including regulating the expression of ABA-independent stress-responsive genes and promoting the synthesis of osmolyte proline to protect plants from water deficit.
Journal of Bioscience and Bioengineering 12/2010; 111(4):478-84. · 1.79 Impact Factor
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ABSTRACT: We cloned the promoter of the 9-cis-epoxycarotenoid dioxygenase gene from Arachis hypogaea L. beta-Glucuronidase (GUS) histochemical staining and GUS activity assay indicated that the activity of the promoter was exhibited predominantly in the leaves and enhanced by water and NaCl stresses, and by application of abscisic acid (ABA) and salicylic acid (SA) in transgenic Arabidopsis. Moreover, two novel ABRE-like (abscisic acid response element) elements were identified in the promoter region.
Bioscience Biotechnology and Biochemistry 10/2009; 73(9):2103-6. · 1.28 Impact Factor
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ABSTRACT: Aldehyde oxidase (AO) plays important role in plant hormone biosynthetic pathways, such as abscisic acid (ABA) and indole-3-acetic acid (IAA). The enzyme catalyzes the last step of the pathways. In this study a full-length cDNA encoding an aldyhyde oxidase was cloned and sequenced from leaves of peanut by RT-PCR, RACE-PCR and genomic DNA walking methods. The full-length cDNA, designated as Arachis hygogaea L. aldehyde oxidase 1 (AhAO1), consists of an open reading frame of 4131 bp, a 326 bp 5' untranslated region and a 128 bp 3' untranslated region including a poly (A) tail of 21 nucleotides. The gene encodes a polypeptide of 1377 amino acids with a calculated molecular weight of 150 kDa and an isoelectric point (pl) of 6.99. Analysis of amino acid sequence of AhAO1 shows that it had 61%, 59% and 55% identity with the AOs from tomato, Arabidopsis and maize, respectively The peanut AO polypeptide contains consensus sequences for iron-sulfur centers and a molybdenum cofactor (MoCo)-binding domain. Semi-quantitative RT-PCR analysis showed that AhAO1 expression was higher in leaves than in roots of peanut.
Journal of Environmental Biology 01/2009; 30(1):93-8. · 0.64 Impact Factor
