Qing-Yi Xie

Hainan University, Haikou, Yunnan, China

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Publications (15)35.26 Total impact

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    ABSTRACT: A Gram-negative, strain M1T, was isolated from sediment of Maar lake in Zhaijian, Guangdong province, China. The diagnostic cell wall diamino acid is meso-diaminopimelic acid, and mycolic acids were not detected. The polar lipid profile of strain M1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. The predominant quinone was MK-7, with MK-6 as minor components. The major fatty acids were anteiso-C15: 0 and anteiso-C17: 0, with iso-C18: 0 as minor components. The DNA G+C content of the genomic DNA is 70.0 mol %. The 16S rRNA gene sequence analysis showed that strain M1T belongs to belongs to the family Dermabacteraceae, and sharing highest sequence similarity with Brachybacterium nesterenkovii JCM 11648T (98.1%). Furthermore, a combination of DNA-DNA relatedness and physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain M1T represents a novel species of the genus Brachybacterium, for which the name Brachybacterium huguangmaarense sp. nov. is proposed. The type strain is M1T (=CCTCC AB 2012866T= DSMZ 26370T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A bacterial strain, designated M26(T), was isolated from a fish gastrointestinal tract, collected from Zhanjiang Port, South China. 16S rRNA gene sequence analysis indicated that strain M26(T) belongs to the subclass α-Proteobacteria, being related to the genus Paracoccus, and sharing highest sequence similarity with Paracoccus alcaliphilus JCM 7364(T) (98.1 %), Paracoccus huijuniae FLN-7(T) (97.3 %), Paracoccus stylophorae KTW-16(T) (97.1 %) and Paracoccus seriniphilus DSM 14827(T) (96.9 %). The major quinone was determined to be ubiquinone Q-10, with Q-9 and Q-8 as minor components. The major fatty acid was identified as C18:1ω7c, with smaller amounts of C18:0 and C16:0. The G+C content of the genomic DNA was determined to be 64.3 mol%. The DNA hybridization value between strain M26(T) and the most closely related type strain, P. alcaliphilus, was 29.0 ± 1.0 %. The results of physiological and biochemical tests and low DNA-DNA relatedness showed that the strain could be readily distinguished from closely related species. On the basis of these phenotypic and genotypic data, strain M26(T) is concluded to represent a novel species of the genus Paracoccus, for which the name Paracoccus siganidrum sp. nov. is proposed. The type strain is M26(T) (=CCTCC AB 2012865(T) = DSM 26381(T)).
    Antonie van Leeuwenhoek 04/2013; · 2.07 Impact Factor
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    ABSTRACT: Strain 268506(T) was isolated from a root of Avicennia marina collected at mangrove forest in Wengchang, Hainan province, China. The 16S rRNA gene sequence of strain 268506(T) showed the highest similarity with Micromonospora equina Y22(T) (98.8 %) and Micromonospora olivasterospora DSM 43868(T) (98.7 %). In addition, gyrB gene phylogeny clearly showed strain 268506(T) should be assigned to the genus Micromonospora but different from any established Micromonospora species. The predominant menaquinones are MK-9(H(8)) and MK-9(H(6)). The major fatty acids are iso-C(16:0), iso-C(15:0) and anteiso-C(17:0). The characteristic whole-cell sugars are xylose, mannose and arabinose. The cell wall contains meso-DAP and glycine. Phosphatidylinositol, diphosphatidylglycerol and phosphatidylethanolamine are the characteristic polar lipids. The DNA G+C content is 70.3 mol%. Some physiological and biochemical properties combined with low DNA-DNA relatedness indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 268506(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora avicenniae sp. nov. is proposed. The type strain is 268506(T) ( = CCTCC AA 2012010(T) = DSM 45758(T)).
    Antonie van Leeuwenhoek 02/2013; · 2.07 Impact Factor
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    ABSTRACT: A novel endophytic actinomycete strain 274745T was isolated from a root of Sonneratia apetala collected at a mangrove forest in Sanya, Hainan province, China. The 16S rRNA gene sequence of strain 274745T showed the highest similarity to Micromonospora pattaloongensis TJ2-2T (98.3%). The phylogenetic analysis based on the gyrB gene also supported the close relationship of these two strains. The predominant menaquinone was MK-10(H8) and the major fatty acids are iso-C15:0, C17:0 and anteiso-C15:0. The characteristic whole-cell sugars were xylose and mannose. The cell wall contained meso-DAP and glycine. The polar lipid profile mainly comprised phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The DNA G+C content is 71.6 mol%. Furthermore, a combination of DNA-DNA relatedness and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 274745T represents a novel species of the genus Micromonospora, for which the name Micromonospora sonneratiae sp. nov. is proposed. The type strain is 274745T (=CCTCC AA2012003T=DSM 45704T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2012; · 2.11 Impact Factor
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    ABSTRACT: A novel actinobacterium strain, 39T was isolated from soil collected from the Barrientos Island, Antarctic. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence revealed that strain 39T represented a novel lineage within the actinobacteria. Strain 39T formed a distinct clade in the family Dermacoccaceae and was most closely related to the members of the genera Demetria (16S rRNA gene sequence similarity, 96.9 %), Branchiibius (95.7 %), Dermacoccus (94.4-95.3 %), Calidifontibacter (94.6 %), Luteipulveratus (94.3%), Yimella (94.2 %) and Kytococcus (93.1 %). The cells of this strain are irregular coccoid to short rod shaped. The peptidoglycan type was A4α with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars contained galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipid and one unknown phospholipid. The acyl type of the cell-wall polysaccharides was N-acetyl. The major cellular fatty acids were anteiso-C17:0 (41.97 %), anteiso-C17:1 w9c (32.16 %) and iso-C16:0 (7.68 %). The DNA G+C content was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov. are proposed. The type strain of Barrientosiimonas humi is 39T (= CGMCC 4.6864T = DSM 24617T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2012; · 2.11 Impact Factor
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    ABSTRACT: An actinomycete strain 232617(T) was isolated from a composite mangrove sediment sample collected in Haikou, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 232617(T) indicated the highest similarity with Micromonospora siamensis TT2-4(T) (99.05%), Micromonospora krabiensis A-2(T) (98.99%) and Micromonospora carbonacea DSM 43815(T) (98.91%). The gyrB gene sequence analysis also indicated that 232617(T) should be assigned to the genus Micromonospora. The cell wall contains meso-DAP and glycine. The major menaquinones were MK-10(H(4)) and MK-10(H(6)), with MK-9(H(4)) as minor components. The characteristic whole-cell sugars are xylose, arabinose and glucose. The phospholipid profile comprises phosphatidylethanolamine, diphosphatidlglycerol and phosphatidylinositol mannoside. The DNA G+C content is 71.5 mol%. Furthermore, a combination of DNA-DNA relatedness and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest related species. On the basis of these phenotypic and genotypic data, strain 232617(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora haikouensis sp. nov. is proposed. The type strain is 232617(T) (= CCTCC AA 201112 (T) = DSM 45626 (T)).
    Antonie van Leeuwenhoek 03/2012; 101(3):649-55. · 2.07 Impact Factor
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    ABSTRACT: An actinomycete strain 234402(T) was isolated from a mangrove soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 234402(T) indicated that the highest similarity was to Verrucosispora sediminis MS426(T) (99.25%). The cell wall contained meso-diaminopimelic acid. The major menaquinones were MK-9(H(4)) and MK-9(H(6)), with MK-9(H(8)) as minor components. The characteristic whole-cell sugars were xylose, mannose and glucose. The phospholipid profile was found to comprise phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unknown phospholipid. The DNA G+C content was 69.2 mol%. The results of physiological and biochemical tests and low DNA-DNA relatedness demonstrated strain 234402(T) could be readily distinguished from the closely related Verrucosispora species. On the basis of these phenotypic and genotypic data, strain 234402(T) represents a novel species of the genus Verrucosispora, for which the name Verrucosispora wenchangensis sp. nov. is proposed. The type strain is 234402(T) (=CCTCC AA 2011018(T)=DSM 45674(T)).
    Antonie van Leeuwenhoek 02/2012; 102(1):1-7. · 2.07 Impact Factor
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    ABSTRACT: Strain 211020(T) was isolated from rhizosphere soil of Excoecaria agallocha in a mangrove in Hainan, China. The strain produced longitudinal pair spores branching from aerial hyphae. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Microbispora, exhibiting the highest 16S rRNA gene sequence similarity (98.75 %) to Microbispora corallina JCM 10267(T) with a low DNA-DNA relatedness value (13±0.6 %). The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid but madurose was not detected. The predominant menaquinones were MK-9(H(4)), MK-9(H(2)) and MK-9(H(0)), and the major fatty acids were iso-C(16 : 0), iso-C(15 : 0) and C(17 : 0). The phospholipid profile of strain 211020(T) comprised phosphatidylinositol mannoside, phosphatidylethanolamine, diphosphatidylglycerol and phospholipids of unknown structure containing glucosamine. The DNA G+C content was 70.8 mol%. On the basis of phenotypic and genotypic data, strain 211020(T) can be distinguished as a novel species of the genus Microbispora, for which the name Microbispora hainanensis sp. nov., is proposed. The type strain is 211020(T) ( = CGMCC 4.5595(T) = DSM 45428(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2011; 62(Pt 10):2430-4. · 2.11 Impact Factor
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    ABSTRACT: A novel strain, 219820(T), whose metabolites were found to be active against tumour cells, was isolated and characterized. The isolate belonged to the genus Streptomyces and had white to grey aerial mycelium and long chains of smooth spores in the aerial mycelium. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 219820(T) had highest similarity to members of the genus Streptomyces and was most closely, albeit loosely, associated with Streptomyces crystallinus NBRC 15401(T) (98.624 % similarity), Streptomyces melanogenes NBRC 12890(T) (98.565 %) and Streptomyces noboritoensis NBRC 13065(T) (98.564 %). However, DNA-DNA relatedness and phenotypic data readily distinguished strain 219820(T) from these phylogenetically related type strains. It is evident from the combination of genotypic and phenotypic data that strain 219820(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces sanyensis sp. nov. is proposed; the type strain is 219820(T) ( = CGMCC 4.5626(T)  = DSM 42014(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 61(Pt 7):1632-7. · 2.11 Impact Factor
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    ABSTRACT: Strain 210417(T), which forms highly branched substrate and aerial mycelia, is a Gram-positive, aerobic and non-motile actinomycete isolated from mangrove rhizosphere soil. 16S rRNA gene sequence analysis showed that the strain should be classified in the genus Nonomuraea, being most closely related to the type strains of Nonomuraea coxensis (99.6 %) and Nonomuraea bangladeshensis (99.3 %). Chemotaxonomic properties [madurose as the major sugar in the cell wall; meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan; MK-9(H(4)) as the major menaquinone; iso-C(16 : 0) (24.1 %) as major fatty acid; and phospholipid pattern type IV] are consistent with the assignment of strain 210417(T) to the genus Nonomuraea. Strain 210417(T) could be differentiated from the closely related species N. coxensis and N. bangladeshensis by morphological, physiological, biochemical and chemotaxonomic properties, 16S rRNA gene sequence analysis and DNA-DNA hybridization results. It is therefore proposed that strain 210417(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea wenchangensis sp. nov. is given; the type strain is 210417(T) ( = CGMCC 4.5598(T)  = DSM 45477(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 61(Pt 6):1304-8. · 2.11 Impact Factor
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    ABSTRACT: A novel endophytic actinomycete, designated strain 202201(T), was isolated from an Acanthus illicifolius root collected from the mangrove reserve zone in Hainan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 202201(T) fell within the family Micromonosporaceae. The strain formed an extensively branched substrate mycelium, which carried uneven warty-surfaced spores. Cell walls of strain 202201(T) contained meso-diaminopimelic acid and xylose, mannose, arabinose, ribose and glucose were detected as whole-cell sugars. The acyl type of the cell-wall polysaccharides was glycolyl. The major menaquinones were MK-9(H(4)), MK-9(H(6)), MK-9(H(8)) and MK-10(H(4)). The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylserine. The major cellular fatty acids were 10-methyl-C(17 : 0), iso-C(15 : 0), iso-C(16 : 0) and C(17 : 1)ω8c. The DNA G+C content was 72.3 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain 202201(T) ( = CGMCC 4.5597(T ) = DSM 45430(T)) represents a novel species of a new genus within the family Micromonosporaceae, for which the name Jishengella endophytica gen. nov., sp. nov. is proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2011; 61(Pt 5):1153-9. · 2.11 Impact Factor
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    ABSTRACT: A Gram-reaction-positive, non-motile actinobacterium, designated strain 210121(T), was isolated from rhizosphere soil of the mangrove fern Acrostichum speciosum. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Asanoa. DNA-DNA relatedness values between strain 210121(T) and the type strains of the three recognized species of the genus Asanoa were below the 70 % threshold recommended for distinguishing bacterial genomic species. The novel isolate contained glutamic acid, glycine, alanine and meso-A(2)pm as cell-wall amino acids, indicating peptidoglycan type A1γ. The characteristic whole-cell sugars were xylose, ribose, glucose and mannose. The predominant menaquinones were MK-9(H(4)), MK-9(H(6)) and MK-9(H(8)). The major fatty acids were iso-C(16 : 0) (30.9 %), C(17 : 0) (23.0 %), anteiso-C(15 : 0) (14.9 %) and iso-C(15 : 0) (12.3 %). The phospholipid profile comprised phosphatidylethanolamine, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine. The G+C content of the DNA was 70.3 mol%. On the basis of the phenotypic and genotypic data, strain 210121(T) ( = CGMCC 4.5593(T)  = DSM 45427(T)) represents a novel species of the genus Asanoa, for which the name Asanoa hainanensis sp. nov., is proposed. An emended description of the genus Asanoa is also proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2010; 61(Pt 10):2384-8. · 2.11 Impact Factor
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    ABSTRACT: Strain 211018(T) was isolated from mangrove Excocaria agallocha rhizosphere soil. 16S rRNA gene sequence analysis showed the highest similarity to the type strains of Micromonospora olivasterospora DSM 43868(T) (98.6 %) and Micromonospora pattaloongensis TJ2-2(T) (98.4 %). gyrB gene sequence analysis also indicated that strain 211018(T) should be assigned to the genus Micromonospora. The characteristic whole-cell sugars are xylose, mannose and arabinose. The predominant menaquinone is MK-9(H(4)) and the major fatty acids are iso-C(15 : 0) (27.5 %), 10-methyl C(17 : 0) (14.2 %), C(17 : 1)ω8c (12.8 %), iso-C(16 : 0) (12.6 %), anteiso-C(15 : 0) (6.1 %), iso-C(17 : 0) (4.1 %) and anteiso-C(17 : 0) (4.0 %). The phospholipid profile comprises phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The DNA G+C content is 70.8 mol%. The chemotaxonomic data of the strain coincided with those of the genus Micromonospora. Furthermore, a combination of DNA-DNA hybridization results and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 211018(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora rhizosphaerae sp. nov. is proposed. The type strain is 211018(T) (=CGMCC 4.5599(T) =DSM 45431(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2010; 61(Pt 2):320-4. · 2.11 Impact Factor
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    ABSTRACT: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.
    Marine Drugs 02/2009; 7(1):24-44. · 3.98 Impact Factor
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    ABSTRACT: We found an error in our paper published in Marine Drugs [1]. [...]
    Marine Drugs 01/2009; 7(4):495-6. · 3.98 Impact Factor

Publication Stats

111 Citations
35.26 Total Impact Points

Institutions

  • 2012–2013
    • Hainan University
      Haikou, Yunnan, China
    • Putra University, Malaysia
      • Department of Biomedical Sciences
      Putrajaya, Putrajaya, Malaysia
    • Wuhan University
      Wu-han-shih, Hubei, China
  • 2010–2013
    • Chinese Academy of Tropical Agricultural Sciences
      Hoihau, Hainan, China