Latifa En-Nanei

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Publications (2)2.38 Total impact

  • Source
    Article: Isolation, drug resistance and molecular characterization of Salmonella isolates in northern Morocco.
    Sanae Ammari, Amine Laglaoui, Latifa En-Nanei, Sophie Bertrand, Christa Wildemauwe, Said Barrijal, Mohammed Abid
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    ABSTRACT: The objective of this investigation was to assess the methods for the characterization of Salmonella isolates and to identify relationships of Salmonella isolates from human and food sources in northern Morocco. Several Salmonella serotypes were isolated from human and food samples and were characterized using conventional culture methods, biochemical, serological, antimicrobial testing, and phage typing. Molecular analyses such as enterobacterial repetitive intergenic consensus (ERIC)-PCR, macrorestriction profiling by pulsed-field gel electrophoresis (PFGE), and virulence gene analysis were also performed. Results: Sixteen Salmonella strains were isolated in our laboratory, serotyped and identified as S. Kottbus, S. Indiana, S. London, S. Typhi, S. Hadar, S. Corvallis, S. Mbandaka, S. Ouakam, S. Tm var. cop., S. Virchow, and S. Altona. The most common resistance profiles for the isolates was ATCFATSCGKSSS, belonging to phage type PT20, ATASCSS associated with strains DT104L/ad and ATATSS for isolates that were not typeable. The PFGE patterns were different for each Salmonella serotype. All strains were negative for the virulence gene spvR. To our knowledge, this is the first molecular characterization of Salmonella in food and humans from Morocco. Comparison of molecular techniques for differentiating between human and food isolates of Salmonella in north of Morocco shows that ERIC typing and PFGE were more discriminating than the other techniques used in this study.
    The Journal of Infection in Developing Countries 01/2009; 3(1):41-9. · 1.19 Impact Factor
  • Source
    Article: Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco.
    Sanae Ammari, Amin Laglaoui, Latifa En-nanei, Sophie Bertrand, Christa Wildemauwe, Said Barrijal, Mohammed Abid
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    ABSTRACT: This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods. Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimicrobial susceptibility testing, and PCR assay targeting the spvR and invA genes. Six fingerprinting profiles were obtained with the ERIC-PCR method, four with PFGE profiling, five with antimicrobial resistance, three with phage typing, and only one with plasmid profiling. spvR gene was detected in six strains, which did not harbour plasmids of 90 kb. The conclusions of this study are drawn from a limited number of isolates. It would be desirable to investigate a greater and more diverse population of Salmonella isolates. S. Enteritidis was genotyped and showed four different patterns by PFGE and six by ERIC-PCR. Accordingly, high genetic similarity and limited genetic diversity were found for these strains from north of Morocco.
    The Journal of Infection in Developing Countries 01/2009; 3(9):695-703. · 1.19 Impact Factor

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