Xinyu Lin

Publications (2)4.27 Total impact

• Article: Comprehensive transcription analysis of human pathogenic fungus Penicillium marneffei in mycelial and yeast cells.

  Xinyu Lin, Yuping Ran, Lantu Gou, Fei He, Ruifeng Zhang, Peng Wang, Yaling Dai
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  ABSTRACT: Penicillium marneffei is a dimorphic fungus that responds to changes in temperature. We performed a comparative analysis of gene expression in mycelial- and yeast-phase P. marneffei cells using high-throughput DNA microarrays. A total of 1,884 differentially expressed genes with annotations in the gene ontology (GO) database were identified between P. marneffei mycelial and yeast cells. These differentially expressed genes mainly belong to 18 categories in the organism's ontology, including reproduction, immunity, metabolism, signaling, etc. Bioinformatics suggests that these differentially expressed genes may help explain the resistance to adverse environments and the virulence of P. marneffei. Nine genes from the results of the DNA microarray experiment were selected for further analysis with real-time quantitative PCR to validate the differential expression. Our data provide a global description of the transcriptional response accompanying adaptation to high temperature in yeast.
  Medical mycology: official publication of the International Society for Human and Animal Mycology 05/2012; 50(8):835-42. · 2.13 Impact Factor
• Article: Arthroderma vanbreuseghemii infection in three family members with kerion and tinea corporis.

  Hao Zhang, Yuping Ran, Yongfang Liu, Ruifeng Zhang, Xinyu Lin, Wei Yan, Yaling Dai
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  ABSTRACT: We present a familial infection caused by Arthroderma vanbreuseghemii. The proband is a 4-year-old boy, who had played with rabbits at his rabbit-farm neighbor. He complained of pruritus and pain in his scalp, which displayed redness, alopecia and painful cysts and eventually discharged pus and scabbed. Several erythema on his face and abdomen were also presented. He was diagnosed as having impetigo but antibacterial agents were not effective and his clinical condition did not improve. Several days later, his parents also developed facial erythema and scaling. The development of a kerion in the boy and tinea corporis in his parents were diagnosed based on the positive KOH examination. Morphologic and biochemical characteristics confirmed that their infections were caused by the zoophilic Trichophyton mentagrophytes, while sequencing of the internal transcribed spacer (ITS) 1/4 polymerase chain reaction products, amplified from primary culture isolates, established its Arthroderma vanbreuseghemii lineage. Random amplified polymorphic DNA (RAPD) analysis indicated these isolates might be the same strain and that infection cruciata occurred in this family. Semi-quantitative analysis of these strains indicated multiple and main enzymatic activities of alkaline phosphatase, beta-glucosaccharase. The boy was cured through treatment with itraconazole 100 mg/day orally in combination with topical washes with 2% ketoconazole shampoo, and his parents were successfully treated by topical application of terbinafine cream.
  Medical mycology: official publication of the International Society for Human and Animal Mycology 01/2009; 47(5):539-44. · 2.13 Impact Factor