Roy Khalaf

Publications (2)3.51 Total impact

• Article: Natural Killer Cell Immunoglobulin-like Receptors (KIR) Genotypes in Follicular Lymphoma patients: Results of a pilot study.

  Roy Khalaf, Rouba Hoteit, Soha Yazbek, Nady El Hajj, Zaher Otrock, Sarah Khansa, Amira Sabbagh, Dina Shammaa, Rami Mahfouz
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  ABSTRACT: AIMS: The Natural Killer Cell Immunoglobulin-like Receptors (KIR) Genotypes profiling in Follicular Lymphoma has not been reported before in the literature. MATERIALS AND METHODS: DNA extracted from 20 Follicular Lymphoma patients and 62 healthy controls was analyzed for KIR genotyping using a polymerase chain reaction / sequence specific primers technique (PCR/SSP) for the presence of 16 KIR genes and pseudogenes loci. RESULTS: The AA, AB, and BB genotypes frequencies were, respectively, 20%, 60% and 20% with an A:B ratio of 1:1. KIR 2DL4, KIR 3DL2, KIR 3DL3, and KIR 3DP1*003 were presented in all individuals. No significant difference between patients and controls was detected. CONCLUSION: KIR genotyping profile does not seem to be associated with Follicular Lymphoma. The results presented in this pilot research represent the first international report about this important clinical entity.
  Gene 05/2013; · 2.34 Impact Factor
• Article: Killer cell immunoglobulin-like receptor (KIR) genotypes in patients with recurrent tonsillitis.

  Mohamad Bitar, Roy Khalaf, Wael Shamseddeen, Nady El Hajj, Georges Ibrahim, Layal Greige, Ghazi Zaatari, Nabil Fuleihan, Amira Sabbagh, Rami Mahfouz
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  ABSTRACT: This study represents the first report on the distribution of killer cell immunoglobulin-like receptor (KIR) genotype among recurrent tonsillitis patients. We recruited 34 Lebanese pediatric patients diagnosed with recurrent tonsillitis and had their DNA typed using sequence-specific primer technique for the presence of 16 KIR loci. We observed that 25 different KIR genotypes were present similar to the general control population with the same KIR gene content. There was no statistically significant difference in the distribution of the activating and inhibitory KIR genes between the two categories. Like in the general control population, we noted a predominance of the AB genotype; however, the KIR genotypic distribution among the tonsillitis patients was much more heterogeneous with even new genotypes not reported in the control group. Although the sample size is small, this first study observes an interesting heterogeneous KIR gene profile in recurrent tonsillitis that warrants larger and further research in the area for the true biological and clinical significance of this observation.
  Genetic Testing 01/2009; 12(4):517-21. · 1.17 Impact Factor