María Inés Roldán

Universitat de Girona, Girona, Catalonia, Spain

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Publications (27)56.54 Total impact

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    ABSTRACT: Le calmar Illex argentinus (Omastrephidae) est une espèce économiquement importante, qui donne lieu à une pêche commerciale intense et de longue durée dans le Sud-Ouest de l’océan Atlantique. Dans ce travail, nous avons étudié la variabilité génétique au travers de l’allozyme (40 loci) et de deux régions mitochondriales (COI (556 bp) et 16S rDNA (439 bp)), dans les deux plus importants stocks reproducteurs, le Summer Spawning Stock (SSS) et le South Patagonic Stock (SPS). Des 20 loci allozymique polymorphiques 5 étaient au 95 % et le taux d’hétérozygotie observé était bas. L’analyse concaténée des deux marqueurs moléculaires mitochondriaux a révélé une diversité haplotypique élevée et une diversité nucléotidique basse. Les données nucléaires et mitochondriales ne révèlent pas de différences génétiques entre les deux stocks reproducteurs, peut-être en raison de la dynamique particulaire de chacun, dont les caractéristiques écologiques et reproductives. Les SSS et SPS stocks reproducteurs de calmar I. argentinus ne peuvent pas être élevés au niveau des stocks génétiques.
    Comptes Rendus Biologies 09/2014; · 1.68 Impact Factor
  • Sandra Heras, María Inés Roldán, Mariano González Castro
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    ABSTRACT: Systematics derived from morphological characters often does not correspond with the evolutionary processes underlying the divergence within a group of organisms. In the family Mugilidae (Teleostei) morphological similarities have resulted in inconsistencies between taxonomy and phylogeny among its species, and particularly for the genera Mugil, Liza and Chelon where both intrageneric and intergeneric phylogenetic clarifications are needed. To address these issues, the direct sequencing of the mitochondrial region that encodes Phenylalanine (69bp), 12S rRNA (842bp), cytochrome c oxidase subunit I (651bp) and cytochrome b (702bp) was carried out. The data reveal that Mugil platanus and Mugil liza represent a continuum of a single species, closely related to but distinct from Mugil cephalus which itself appears to comprise a grouping of multiple and closely related species. This species complex was genetically distinct from Mugil curema, which, based on three clearly diverged species identified in this study along the Atlantic coast of the Americas, requires extensive taxonomic revision throughout its world-wide distribution. Unlike the monophyly supported within Mugil, relationships within Liza are paraphyletic, and a taxonomic revision of the genera Liza, Chelon and Oedalechilus is needed.
    Reviews in Fish Biology and Fisheries 08/2014; 19(2):217-231. · 2.56 Impact Factor
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    ABSTRACT: The small-spotted catshark Scyliorhinus canicula is a small demersal chondrychtian distributed on continental shelf and uppermost slope waters of the Mediterranean Sea and north eastern Atlantic Ocean. It has commercial value in some European regions, whereas in other it is considered a bycatch species. Species’ genetic structure was analysed by means of 578bp mitochondrial COI sequences. A total of 192 individuals (122 obtained in the present work and 70 retrieved from GenBank) from 11 Mediterranean and 1 Atlantic locations were considered. Overall, we detected 25 COI haplotypes, seven of which were newly found. Moreover, a high number of haplotypes were location- and/or region-private. Low values of nucleotidic diversity (total  = 0.0024) and moderate to high haplotypic diversity (h = 0.500 to 0.907, total h = 0.810) were found. Significant genetic structuring in the study area was highlighted by AMOVA, -statistics and Bayesian assignment analyses. The Atlantic sample was genetically divergent from Western Mediterranean counterparts and the Adriatic samples diverged from Eastern Mediterranean ones. Instead, Western and Eastern Mediterranean were not significantly divergent, suggesting that the Strait of Sicily is not effective in restricting past or current gene flow. No pattern of isolation by distance was detected. From a fisheries perspective, our results represent the first evidence of genetic structuring in S. canicula and are consistent with the presence of multiple genetic stocks in the study area. Further genetic analyses coupled with a fine grained sampling design are needed to precisely identify the borders of genetic stocks. These data provide a significant contribution for the planning of a long-term effective management policy, which could ensure sustainability of resource exploitation and stock viability.
    Fisheries Research 03/2014; 154:11-16. · 1.84 Impact Factor
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    ABSTRACT: Phylogeographical studies can reveal hidden patterns in the evolutionary history of species. Comparative analyses of closely related species can further help disentangle the relative contributions of processes responsible for such patterns. In this work, the phylogeography of two aristeid species, Aristeus antennatus and Aristaeomorpha foliacea, was compared through multiple genetic markers. These marine shrimp species are of high commercial importance, and are exploited in the Mediterranean Sea (MED) and in Mozambique Channel (MOZ) where they occur in partial sympatry. Aristeus antennatus (N = 50) from Western and Eastern Mediterranean (WM and EM, respectively), Atlantic Ocean (AO) and MOZ, and Aristaeomorpha foliacea (N = 40) from WM, EM, MOZ North-Western Australia (AUS) were analyzed with two nuclear genes (PEPCK and NaK) and one mitochondrial (COI) gene. Within the study area differences were found between the two species in their phylogeographical patterns, suggesting distinct responses to environmental changes. Monophyly of Aristeus antennatus was found across its distributional range. This pattern contrasted by a deep evolutionary split within Aristaeomorpha foliacea where genetic diversity followed geography distinguishing MED-MOZ and AUS. We propose that the AUS lineage of A. foliacea warrants consideration as a distinct species, with consequent implications in systematics and resource management.
    PLoS ONE 03/2013; 8(3):e59033. · 3.53 Impact Factor
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    ABSTRACT: The giant red shrimp, Aristaeomorpha foliacea, is a commercially important species in the Mediterranean Sea (MED), Mozambique Channel (MOZ), and north western Australia (AUS). 685 bp of the mitochondrial COI gene was sequenced in 317 individuals from six Mediterranean and two Indian Ocean localities. Genetic diversity estimates of Indian Ocean samples were higher than those of MED counterparts. AMOVA, phylogenetic tree, haplotype network and Bayesian assignment analyses detected three haplogroups, corresponding to MED, MOZ and AUS, separated by three and 38 mutational steps, respectively. Within MED shallow genetic divergence between populations was dependent on local oceanographical characteristics. Mismatch distribution analysis and neutrality tests provided a consistent indication of past population expansion in each region considered. Our results provide the first evidence of genetic structure in A. foliacea and suggest a scenario of allopatric speciation within the Indian Ocean that, however needs deeper examination.
    Journal of Sea Research 02/2013; 76:146-153. · 1.86 Impact Factor
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    ICES Journal of Marine Science 01/2013; · 2.53 Impact Factor
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    PLoS ONE 01/2013; · 3.53 Impact Factor
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    ABSTRACT: Meristic and different morphometric approaches were employed to assess the discrimination of 7 species of Mugilidae fishes (Mugil cephalus, M. liza, M. curema, M. hospes, Liza aurata, L. ramada, and Chelon labrosus), but also to contribute to a better understanding of body-shape differences among this valuable species group. Three types of variables and their corresponding morphometric approaches were employed: 1) linear morphometrics measurements (LMMs); 2) interlandmark distances (IlDs); and 3) coordinate data (landmarks). Before the analyses, data exhibiting allometric growth were normalized. Data analysis included a one-way ANOVA (meristic data), a principal component analysis (PCA), and a cross-validated discriminant analysis (DA). The ANOVA showed significant differences in both lateral and transverse series scales. The PCA based on LMMs allowed the characterization of 6 groups, although some overlap between them was detected. The DA correctly classified 68.4% of the fishes according to their LMMs. The centroids of the 8 groups were separated for both the 1st and 2nd discriminant functions. The morphometric analysis based on IlDs yielded the best discrimination rates of the 3 approaches employed (96% for the DA). In the geometric morphometric analysis, the DA correctly classified 83.8% of the fishes according to their body shape. Although 8 groups were defined, some overlap among samples was detected. Mugil hospes was the best defined and most isolated species as observed in both the PCA and DA. Interestingly, the 3 morphometrics approaches employed separated M. curema specimens in 2 groups (Argentinean and Mexican samples). Moreover, European and Mexican samples of M. cephalus plotted separately in the PCA of the LMM- and IlD-based approaches. These shape differences among M. curema of Argentina/Mexico and M. cephalus of Europe/Mexico reinforce the current hypothesis of a species complex, or even undescribed species as previously suggested by the authors.
    Zoological studies 12/2012; 51(8):1515. · 1.01 Impact Factor
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    ABSTRACT: This work reports the first genetic data of Aristaeomorpha foliacea, a marine decapod of high commercial value, from six Mediterranean localities and one new fishing ground in the Mozambique Channel. The use of five Inter Simple Sequence Repeat (ISSR) primers provided 150 polymorphic loci. Average estimates of genetic diversity did not significantly differ among sampled localities, with a mean value of heterozygosity H=0.105±0.015. Analysis of molecular variance (AMOVA) allocated>98% of genetic variability to the within-sample component, displaying values higher than those previously reported in ISSR studies on marine invertebrates. Cluster analyses did not detect geographically or genetically distinct groups. The observed lack of large-scale genetic differentiation is discussed in relation to the high potential for larval dispersal of the species and to features of the marker employed.
    Comptes rendus biologies 10/2011; 334(10):705-12. · 1.71 Impact Factor
  • Sandra Heras, María Inés Roldán
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    ABSTRACT: This contribution provides an insight into Atheriniformes systematics based on four mitochondrial regions: 12S rRNA, cytb, COI and control region (2794bp in total). In the Atherinopsoidei (New World silversides), comparisons among five species of Odontesthes, O. argentinensis, O. bonariensis, O. smitti, O. hatcheri and O. incisa revealed a putative marine-freshwater pairing pattern of Odontesthes species, possibly driven by sea level fluctuations of South American waters. This study represents the first data on molecular phylogeny of Odontesthes species that can be of usefulness to biodiversity conservation policies. In the Atherinoidei (Old World silversides), Atherina boyeri was corroborated as a species complex constituted by a marine form, a marine with dark spots form and a brackish form. Concretely, Odontesthes and Atherina may represent geographically replicated models to study genetic adaptation and speciation of marine species to brackish and freshwater habitats. In addition, phylogenetic analyses supported Odontesthes and Atherina as monophyletic taxa and their separation into two differentiated suborders Atherinopsoidei and Atherinoidei, respectively.
    Comptes rendus biologies 04/2011; 334(4):273-81. · 1.71 Impact Factor
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    Marine Ecology Progress Series 01/2011; 421:163-171. · 2.64 Impact Factor
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    ABSTRACT: The taxonomic status of hake (Merluccius spp.) in the northeastern Pacific is unclear. Hakes in this region are Merluccius productus, M. angustimanus, M. hernandezi, and a morphotype known as dwarf hake. Of these, only the first two species are currently valid. Descriptions in previous studies have been limited by overlapping morphological characteristic, lack of biological material, and limited numbers of sampling localities. To clarify their taxonomy, 461 hake were obtained from eight localities along the North American coast for morphological and mitochondrial DNA sequence analyses (cytochrome b, cytochrome c oxidase subunit I, and 16S ribosomal rDNA). Morphological and molecular analyses suggest that hake in this region represent a continuum of a single species with some levels of morphological and genetic intra-specific variation. In light of these results, we propose that M. productus is the only species of hake present along the North American and northern Central American coast. Keywords Merluccius –Northeastern Pacific–Morphology–Cytochrome b –Cytochrome c oxidase subunit I–16S rDNA
    Reviews in Fish Biology and Fisheries 01/2011; 21(2):259-282. · 2.56 Impact Factor
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    Scientia Marina 08/2010; 74:569-575. · 1.25 Impact Factor
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    ABSTRACT: In this paper we addressed the phylogeographical genetic structure of the economically important red shrimp, Aristeus antennatus (Crustacea, Aristeidae) in the Western Mediterranean. Partial mitochondrial regions of the cytochrome c oxidase subunit I (514 bp) and ribosomal 16S subunit (547 bp) were sequenced in 137 individuals collected at three localities: Catalan Sea, Ligurian Sea and the southern Tyrrhenian Sea. Values of haplotypic diversity were h = 0.552-0.724, whereas those for nucleotide diversity were pi = 0.0012-0.0026. Among-sample genetic diversity was not significant and no geographical patterns in the distribution of haplotypes were apparent. Results of the present study are consistent with a past population expansion that occurred <2,000 years ago. Despite the current fishing pressure, genetic variability appears to be sufficiently high to keep A. antennatus populations stable over time. Dispersal-related life history traits may account for the shallow genetic structure. Our results are not in contrast with the hypothesis of sustainability of Western Mediterranean red shrimp fisheries predicted on the basis of previously obtained biological results.
    Genetica 11/2008; 136(1):1-4. · 1.75 Impact Factor
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    ABSTRACT: Conservative morphological characters make identification of mullet species difficult. As a consequence, cosmopolitan distribution of Mugil cephalus is currently under discussion. In order to clarify the controversy regarding the taxonomic status of the southern Atlantic American mullet M. platanus, in relation to Mugil cephalus, a comprehensive analysis is presented using sequences of the mitochondrial gene cytochrome b, landmark‐based morphometry and meristic data. The interlandmark distances showed differentiation between individuals of M. platanus and M. cephalus analyzed. Variables, representing the height at different levels of the longitudinal axis of the body, exposed that M. platanus has more robust middle and caudal segments of the body, in a lateral view, with respect to M. cephalus. Transversal series scales have not been useful for the identification of species. Lateral series scales seem to be useful to differentiate species, but taking into account that range showed an overlapped gradual variation. Genetic distance obtained between species shows a typical intrageneric level comparison. Two clear phylogroups have been detected indicating a high degree of genetic isolation between both species. Recognition of M. platanus as valid allopatric species is suggested.
    Italian Journal of Zoology 09/2008; 75(3):319-325. · 0.87 Impact Factor
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    ABSTRACT: Abstract –  Conservation of genetic diversity within species requires effective management techniques. To study the spatial structure of the killifish Aphanius iberus and to improve ongoing conservation practices, we used genetic approaches to assess the genetic structure of this endemic Iberian species. Twenty locations were analysed by allozyme electrophoresis and allelic variation was detected at 11 of 17 loci examined. Results of our study enabled us to redefine its conservation policy based on Operational Conservation Units (OCUs), which were proposed in 1996. The new OCUs recognise the genetic isolation of some marshland populations (FST = 0.247) and incorporate reintroductions that occurred over the last 10 years. We also propose that the status of A. iberus in the International Union for Conservation of Nature and Natural Resources (IUCN) Red List of Threatened Species be changed from data deficient to endangered. Our work exemplifies how temporal genetic monitoring of populations can be a powerful tool to improve conservation programmes.
    Ecology of Freshwater Fish 05/2007; 16(2):257 - 266. · 1.94 Impact Factor
  • Sandra Heras, Mariano González Castro, María Inés Roldán
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    ABSTRACT: A previously unidentified mullet species (Mugil sp.) from Mar Chiquita lagoon, Argentina, was compared with two candidate species Mugil curema and M. cephalus using 12 truss landmarks and three mitochondrial DNA genes (12S rRNA, cytochrome b and COI). Both procedures confirmed that the Argentinean fish were white mullet M. curema. Morphological comparisons clearly segregated M. curema from M. cephalus samples in PCA plot. Similarly, Argentinean haplotypes at each gene overlapped with M. curema reference samples while M. cephalus haplotypes remained distinct. These combined data provide a valuable baseline for further investigations on the geographic distribution of this commercially important species group.
    Aquaculture 11/2006; · 1.83 Impact Factor
  • J. M. Pujolar, M. I. Roldán, C. Pla
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    ABSTRACT: Bonito Sarda sarda samples collected in three areas from the Mediterranean Sea exhibited considerable genetic heterogeneity over all locations. This provided preliminary evidence for two different groups of bonito, one for the Ligurian and the Ionian Seas and the other for the Aegean Sea.
    Journal of Fish Biology 04/2005; 59(1):169 - 174. · 1.73 Impact Factor
  • J. M. Pujolar, M. I. Roldán, C. Pla
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    ABSTRACT: The genetic population structures of Atlantic northern bluefin tuna ( Thunnus thynnus thynnus) and albacore ( T. alalunga) were examined using allozyme analysis. A total of 822 Atlantic northern bluefin tuna from 18 different samples (16 Mediterranean, 1 East Atlantic, 1 West Atlantic) and 188 albacore from 5 samples (4 Mediterranean, 1 East Atlantic) were surveyed for genetic variation in 37 loci. Polymorphism and heterozygosity reveal a moderate level of genetic variability, with only two highly polymorphic loci in both Atlantic northern bluefin tuna ( FH* and SOD- 1*) and albacore ( GPI- 3* and XDH*). The level of population differentiation found for Atlantic northern bluefin tuna and albacore fits the pattern that has generally been observed in tunas, with genetic differences on a broad rather than a more local scale. For Atlantic northern bluefin tuna, no spatial or temporal genetic heterogeneity was observed within the Mediterranean Sea or between the East Atlantic and Mediterranean, indicating the existence of a single genetic grouping on the eastern side of the Atlantic Ocean. Very limited genetic differentiation was found between West Atlantic and East Atlantic/Mediterranean northern bluefin tuna, mainly due to an inversion of SOD- 1* allele frequencies. Regarding albacore, no genetic heterogeneity was observed within the Mediterranean Sea or between Mediterranean and Azores samples, suggesting the existence of a single gene pool in this area.
    Marine Biology 01/2003; 143(3):613-621. · 2.39 Impact Factor
  • J.M Pujolar, M.I Roldán, C Pla
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    ABSTRACT: Nine samples of swordfish from different Mediterranean areas plus a sample obtained in Atlantic waters adjacent to the Strait of Gibraltar were examined for temporal and spatial heterogeneity using allozyme analysis. Only two out of 38 loci (IDHP-2* and LDH-3*) were polymorphic based on a frequency of less than 0.95 in one or more samples. Both polymorphism and heterozygosity revealed a low genetic variability for swordfish in comparison with other pelagic species. No heterogeneity due to differences between age groups was observed for those samples including juvenile and adult specimens. The lack of temporal or spatial heterogeneity found in this study is consistent with a single population of swordfish in the Mediterranean Sea and adjacent waters of the Atlantic Ocean.
    Journal of Experimental Marine Biology and Ecology 09/2002; · 2.48 Impact Factor

Publication Stats

212 Citations
56.54 Total Impact Points

Institutions

  • 1998–2014
    • Universitat de Girona
      • Department of Biology
      Girona, Catalonia, Spain
  • 2006
    • Universidad Nacional de Mar del Plata
      • Departamento de Ciencias Marinas
      Mar del Plata, Provincia de Buenos Aires, Argentina