[show abstract][hide abstract] ABSTRACT: Abstract Objective: The study aimed to investigate a possible relationship between second trimester aneuploidiy screening results and oxidative stress in foetal amnion and maternal serum. Methods: Concerning the outcome of the second-trimester screening test, 50 pregnant women of high risk were included in the experimental group, whereas 50 pregnant women with normal scores who wished to proceed with the amniocentesis procedure due to advanced maternal age and counselling were included in the control group. The biochemical parameters of total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were measured in the amniotic fluid samples and maternal serum samples. Results: OSI in the maternal serum samples, as well as TAS and TOS in the amniotic fluid, was significantly higher in the control group compared to the experimental group (p<0.001, p=0.047, p=0.005, respectively). There was no significant difference in the TAS and TOS in the maternal serum samples or the OSI in the amniotic fluid between the groups. Conclusions: The results indicate that the positivity of the screening test is not significantly correlated with oxidative stress, a factor regarded as a pathological mechanism in various diseases. Potential maternal anxiety could underlie the elevated oxidative stress in the control group.
The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 09/2013; · 1.36 Impact Factor
[show abstract][hide abstract] ABSTRACT: To evaluate the results and complications of myomectomy carried out during caesarean section.
A retrospective study of 16 patients who underwent myomectomy concurrently with caesarean section in our clinic between January 2009 and September 2012 was conducted. The pre- and postoperative haemoglobin values, number, size and total volume of excised fibroid nodules, location of fibroids, duration of operation, and duration of hospital stay of all patients were retrospectively investigated.
While the most common leiomyoma was transmural myoma, with ten cases encountered, the most common location was in the corpus anterior, where transmural myomas were seen in five patients. The volume of the excised leiomyomata ranged from 84 to 3.300 cm³. The average preoperative haemoglobin value of our patients was 11.4 g/dl, while the postoperative value was 10.3 g/dl. Of 16 patients included in the study, two required blood transfusions due to excessive bleeding. Uterine defects caused by the myomectomy were closed without problems in all patients, and no patient required a hysterectomy. The average time for the myomectomy and caesarean section procedure was 56.1 min. All patients were discharged without problems an average of 3.25 days after the operation.
Myomectomy carried out during caesarean section is a trusted surgical intervention regardless of the size of leiomyomata.
Archives of Gynecology 09/2013; · 0.91 Impact Factor
[show abstract][hide abstract] ABSTRACT: Oxidative stress has been shown to play an important role in the pathogenesis of pre-eclampsia, and DNA damage frequently occurs in cells exposed to such stress. The aim of the present study was to investigate DNA damage and oxidative stress in mildly pre-eclamptic women and their offspring.
We studied 25 mildly pre-eclamptic mothers, 20 healthy controls, and their infants. Mononuclear leukocyte DNA damage, total antioxidant status (TAS), and total oxidant status (TOS) were determined and the oxidative stress index (OSI) was calculated.
DNA damage, and TOS and OSI levels were significantly increased, and TAS levels significantly decreased, in maternal and cord blood samples of the mildly pre-eclamptic group. A positive correlation between the extent of DNA damage and diastolic blood pressure was evident in pre-eclamptic mothers and there was a negative correlation between the extent of DNA damage and TOS.
Both oxidative stress and DNA damage are elevated in mildly pre-eclamptic patients and their offspring. Increased oxidative stress may be important in inducing DNA damage in pre-eclamptic patients.
European journal of obstetrics, gynecology, and reproductive biology 08/2013; · 1.97 Impact Factor
[show abstract][hide abstract] ABSTRACT: Anesthetic agents might considerably influence maternal-fetal oxidative stress and antioxidants during cesarean section (CS). The aim of this study was to investigate the effects of desflurane and sevoflurane on oxidative stress parameters both in mothers and newborns undergoing elective CS.
Eighty ASA physical status I-II, term parturients undergoing elective CS under general anesthesia were randomized to desflurane (Group D) and sevoflurane (Group S) groups. Blood samples were collected from mothers before operation and postoperatively and umbilical artery samples were obtained at delivery. Total oxidant status (TOS), total antioxidant capacity (TAC) status, lipid hydroperoxide (LOOH), and free sulfhydryl (-SH) levels were measured and oxidative stress index was calculated. Secondary outcomes included maternal hemodynamics.
Preoperative LOOH, TOS, OSI, TAC, and -SH levels were similar among groups. Postoperative maternal serum LOOH, TOS, and OSI levels were significantly increased in Group D compared to Group S (p = 0.003, p = 0.005, p = 0.04; respectively). Postoperative umbilical artery LOOH, TOS, OSI levels were also significantly increased in Group D compared to Group S (p = 0.04, p = 0.02, p = 0.01; respectively). Postoperative TOS (p = 0.001, < 0.001 respectively) and OSI (p = 0.003, < 0.001 respectively) levels in both Group D and Group S were statistically significantly decreased compared to preoperative levels. Postoperative LOOH and -SH levels in Group S (p = 0.04, 0.029 respectively) were statistically significantly decreased compared to preoperative levels. There were no significant differences in TAC and -SH levels among groups (p = nonsignificant [n.s.]). Maternal perioperative mean blood pressure and heart rate were similar among groups (p = n.s.).
Oxidative stress indices might be modified with preferred anesthetic agent and sevoflurane showed more favorable effects than desflurane in view of oxidative stress.
Wiener klinische Wochenschrift 07/2013; · 0.81 Impact Factor
[show abstract][hide abstract] ABSTRACT: Contrary effects of vitamin B12 deficiency have been shown on the cardiovascular system. Aim of our study was to analyze left ventricular (LV) myocardial deformation, by use of two dimensional (2D) speckle tracking echocardiography (STE) in patients with vitamin B12 deficiency and normal LV ejection fraction. : METHODS: Twenty-five patients with vitamin B12 deficiency (B12 levels < 200 pg/ml; mean age: 29.6 ± 8.2 y, 15 female), and 27 healthy controls (B12 levels > 200 pg/ml; mean age: 30.1 ± 6.9 y, 13 female) were included in the study. 2D echocardiography images were transferred to a workstation for further offline analysis. Longitudinal peak systolic (LPSS) and global strain (LGS) was obtained from four chamber and apical long axis (APLAX) views. : RESULTS: Standard echocardiographic parameters and tissue Doppler imaging (TDI) velocities were comparable between groups. All LPSS values in the patient group except for apical 4C septal wall longitudinal strain were significantly decreased than those in the control group. There was a positive correlation between B12 levels and strain values except apical 4C septal wall strain values.
We found that in patients with B12 deficiency, global and segmental myocardial deformation : was impaired and this impairment was correlated with the levels of vitamin B12.
[show abstract][hide abstract] ABSTRACT: BACKGROUND AND OBJECTIVE. Oxidative stress is believed to play a role in the development of preeclampsia (PE). It is known that an increased cystatin C level is also associated with PE. The aim of this study was to investigate the relationship between oxidative stress parameters and cystatin C levels in patients with severe PE. MATERIAL AND METHODS. Forty-four patients with severe PE and 40 healthy pregnant women were recruited for the study. All study subjects were divided into 2 groups: group 1 (n=44) consisted of patients with severe PE, and group 2 (n=40) included healthy pregnant subjects. Blood samples were obtained from all subjects in order to measure the cystatin C level, total antioxidant status, and total oxidant status. The oxidative stress index was calculated. RESULTS. The group 1 had significantly higher cystatin C, total oxidant status, oxidative stress index levels and lower total antioxidant status level as compared with the group 2 (P=0.001, P<0.001, P<0.001, P=0.036, respectively). The serum cystatin C level was significantly correlated with the oxidative stress index (r=0.609, P<0.001). CONCLUSIONS. The present study demonstrated that both oxidative stress and cystatin C levels were increased in patients with PE, and the increased cystatin C levels seem to be a consequence of oxidative stress.
[show abstract][hide abstract] ABSTRACT: Justiﬁcativa e objetivos
: Nosso objetivo foi investigar o efeito de 21% e 40% de oxigênio suplementar sobre o estresse oxidativo materno e neonatal em cesariana eletiva (CE) sob raquianestesia.
: Foram incluídas no estudo 80 parturientes com gestação a termo, submetidas à CE sob raquianestesia. As pacientes foram randomicamente alocadas em dois grupos para receberem 21% (grupo Ar) ou 40% (grupo oxigênio) de oxigênio a partir do momento da incisão até o ﬁm da cirurgia. Amostras de sangue das parturientes e da artéria umbilical (AU) foram coletadas antes e depois da cirurgia. A capacidade antioxidante total (CAT), o estado oxidante total (EOT) e o índice de estresse oxidativo (IEO) foram medidos.
: Idade, peso, altura, paridade, semana de gestação, tempo de incisão espinhal, tempo de incisão cirúrgica para extrair o feto, tempo de extração do feto, tempo de cirurgia, escores de Apgar no primeiro e quinto minutos e peso ao nascer foram semelhantes entre os grupos (p > 0,05 em todas as comparações). Não houve diferença entre os grupos em relação aos níveis pré-operatórios de CAT, EOT e IEO (p > 0,05 em todas as comparações). Os níveis maternos pós- operatórios de CAT, EOT e IEO aumentaram signiﬁcativamente no grupo oxigênio (p = 0,047; < 0,001 e 0,038, respectivamente). Nas artérias umbilicais, os níveis da CAT aumentaram signiﬁcativamente no grupo oxigênio (p = 0,003) e os de EOT e IEO aumentaram signiﬁcativamente no grupo Ar (p = 0,02 e < 0,001, respectivamente).
: A diferença em relação ao impacto sobre o estresse oxidativo materno e fetal da suplementação de 40% em comparação com a de 21% exige estudos adicionais em ampla escala que investiguem o papel da suplementação de oxigênio durante CE sob raquianestesia.
Brazilian Journal of Anesthesiology. 01/2013; 63(5):393–397.
[show abstract][hide abstract] ABSTRACT: The aim of this study was to investigate oxidative stress markers and prolidase activity in serum and tissue samples of women with uterine fibroids, with further analysis on position and size. Lipid hydroperoxide, ceruloplasmin, catalase, arylesterase, free sulfhydryl group activity and prolidase activity levels were measured in fibroid tissue, myometrial tissue and serum of the same patients (n = 51), at the same time. Results show that ceruloplasmin, catalase, arylesterase, free sulfhydryl group and prolidase activities were higher in fibroid tissue than those in myometrial tissue (p = 0.003, 0.009, 0.004, 0.02, 0.008, respectively). Serum levels of catalase and prolidase were lower, and arylesterase and free sulfhydryl groups were higher in the fibroid group than those in the control group (p < 0.001 for all). Fibroid volume in submucosal subgroup of the fibroid group yield significant correlation with ceruloplasmin, catalase, arylesterase and prolidase activities (r = 0.84, p = 0.02; r = 0.93, p < 0.001; r = 0.63, p = 0.049 and r = 0.87, p = 0.01, respectively). Despite the lack of statistical significance, the highest levels of prolidase activity were found in fibroid samples, especially in submucosal ones. It is concluded that this study demonstrated increased antioxidative repair system in the fibroid tissue compared to the myometrium and serum of the same patients. Additionally, higher pathophysiological potential of the submucosal fibroids over intramural and subserosal fibroids were shown with the levels of oxidative stress markers and prolidase activity levels.
Journal of obstetrics and gynaecology: the journal of the Institute of Obstetrics and Gynaecology 01/2012; 32(1):68-72. · 0.43 Impact Factor
[show abstract][hide abstract] ABSTRACT: Aim: To investigate serum paraoxonase and arylesterase activities, and lipid hydroperoxide levels in pregnant women with iron deficiency anemia. Materials and methods: Paraoxonase and arylesterase activities, and lipid hydroperoxide levels were assessed for pregnant women with iron deficiency anemia (n = 50) and controls (n = 70). Serum basal and salt-stimulated paraoxonase, and arylesterase activities were measured spectrophotometrically. The lipid hydroperoxide levels were measured by ferrous ion oxidation xylenol orange assay. In addition, lipid parameters were determined by routine laboratory methods. Results: Basal and salt-stimulated paraoxonase, and arylesterase activities were significantly lower (P = 0.026, P = 0.031, and P = 0.018, respectively) in pregnant women with iron deficiency anemia when compared to the controls, while lipid hydroperoxide levels were significantly higher (P = 0.004). A significant positive correlation was found between paraoxonase activity and hemoglobin levels (r = 0.329, P = 0.020), while there was inverse correlation between lipid hydroperoxide and hemoglobin levels (r = -0.457, P = 0.001). Among iron deficiency anemia, serum paraoxonase activity was inversely correlated with lipid hydroperoxide levels (r = -0.535, P < 0.001). Conclusion: The findings of the present study have shown that diminished serum paraoxonase and arylesterase activities, and increased lipid hydroperoxide levels may play a role in the early pathogenesis of atherosclerotic heart disease in pregnant women with iron deficiency anemia. Gebelikte demir eksikliği anemisinde serum paraoksanaz ve arilesteraz aktiviteleri Amaç: Demir eksikliği anemisi olan gebe kadınlarda serum paraoksanaz ve arilesteraz aktiviteleri ve lipit hidroperoksit seviyelerini araştırmaktır. Yöntem ve gereç: Paraoksanaz ve arilesteraz aktiviteleri ve lipit hidroperoksit seviyeleri demir eksikliği anemisi olan (n = 50) ve olmayan (n = 70) gebe kadınlarda değerlendirildi. Serum bazal ve tuz paraoksanaz ve arilesteraz aktiviteleri spektrofotometrik olarak ölçüldü. Lipit hidroperoksit seviyeleri xylenol orange mevcudiyetinde demir iyon oksidasyonuyla ölçüldü. Lipit profili ise rutin laboratuar yöntemleriyle çalışıldı. Bulgular: Bazal ve tuz ile uyarılan paraoksanaz ve arilesteraz aktiviteleri kontrol ile karşılaştırıldığında demir eksikliği anemisi bulunan gebelerde anlamlı derecede azalmasına rağmen (sırasıyla, P = 0,026, P = 0,031, ve P = 0,018), lipit hidroperoksit seviyeleri anlamlı derecede yüksek bulundu (P = 0,004). Hemoglobin seviyesi ve lipit hidroperoksit seviyeleri arasında negatif korelasyon varken (r = -0,457, P = 0,001), paraoksanaz aktivitesi ve hemoglobin düzeyi arasında pozitif korelasyon bulundu (r = 0,329, P = 0,020). Demir eksikliği anemisi bulunan gebelerde, serum paraoksanaz aktivitesi lipit hidroperoksit seviyeleri ile negatif korelasyona sahipti (r = -0,535, P < 0,001).
[show abstract][hide abstract] ABSTRACT: To compare the levels of serum prolidase activity and oxidative stress markers including total oxidative status (TOS), total antioxidant capacity (TAC), lipid hydroperoxide (LOOH) and total free sulfhydryl (-SH) in healthy pregnant women without early pregnancy loss (EPL) and women with EPL.
Serum samples were obtained from 45 healthy first-trimester pregnancies and 45 pregnancies complicated with EPL. We have measured serum prolidase activity, TAC, TOS, -SH and LOOH levels spectrophotometrically.
Serum prolidase, TAC and -SH levels were significantly lower in the women with EPL than in the women without EPL (p <0.001, p = 0.001, p < 0.001, respectively), whereas TOS and LOOH levels were significantly higher (p < 0.001, p = 0.004, respectively). Prolidase activity was negatively correlated with TOS and LOOH levels (r = -0.299, p = 0.004; r = -0.323, p = 0.002, respectively), while positively correlated with TAC and -SH levels (r = 0.232, p = 0.028, and r = 0.418, p <0.001, respectively)
Findings of this study have shown that serum prolidase activity and oxidative stress are significantly associated with the presence of EPL, and the correlation between serum prolidase activity and the markers of oxidative stress was reflected in increased serum TOS and LOOH levels and decreased serum TAC and -SH levels.
Gynecologic and Obstetric Investigation 12/2009; 69(2):122-7. · 1.10 Impact Factor
[show abstract][hide abstract] ABSTRACT: Umbilical metastasis (Sister Mary Joseph's nodule) of malignant neoplasms is a rare condition. These nodules usually arise from the gastrointestinal or genitourinary tract and may present the first sign of a previously unknown primary tumor. We describe a 49-year-old woman presenting with Sister Mary Joseph's nodule as the first sign of an extremely aggressive Stage IV mixed type epithelial ovarian carcinoma, who died 15 months after the initial diagnosis. This is the first case of a Sister Mary Joseph's nodule from a serous component of a Stage IV mixed type epithelial ovarian cancer.
European journal of gynaecological oncology 01/2009; 30(4):455-7. · 0.58 Impact Factor
[show abstract][hide abstract] ABSTRACT: Background. Oxidative stress is believed to have a role in the development of preeclampsia (PE). It is known that an increased ceruloplasmin (CP) level is also associated with PE. The aim of this study was to investigate the relationship between oxidative stress parameters and CP levels in patients with severe PE. Patients and Methods. Sixty patients with severe PE and 60 healthy pregnant women were recruited to the study. All study subjects were divided into 2 groups; group 1(n=60) consisted of patients with severe PE, and group 2 (n=60) consisted of healthy pregnant subjects. Blood samples were obtained to measure CP, total antioxidant status and total oxidant status from all subjects. Oxidative stress index was calculated. Results. Compared to group 2; group 1 had significantly higher CP, total oxidant status, oxidative stress index and lower total antioxidant status levels (p<0.001, p<0.001, p=0.001, p=0.008, respectively). Serum CP levels were significantly correlated with oxidative stress index levels (r=0.385, p=0.002). Conclusion. The present study demonstrated that both oxidative stress and CP levels increased in patients with PE, and increased CP levels seem to be a consequence of oxidative stress. Clin Ter 2013; 164(2):e83-87. doi: 10.7417/CT.2013.1536.
La Clinica terapeutica 164(2):e83-e87. · 0.33 Impact Factor