[Show abstract][Hide abstract] ABSTRACT: In this study, samples of raw ground beef (n = 150) and fresh sausage (n = 100) were collected randomly from butcheries, supermarkets, and fast-food shops, in Casablanca, Morocco. Two types of meat product samples were considered, one with spices (n = 115) and other without spices (n = 135). All the samples were analyzed for the presence of the following bacteria: Escherichia coli, Staphylococcus, Clostridium perfringens, Salmonella, and Listeria monocytogenes. E. coli strains were further typed by pulsed-field gel electrophoresis (PFGE), Operon O, and characterized for virulence genes by polymerase chain reaction (PCR). Results indicated that counts of E. coli, coagulase-positive Staphylococcus, and C. perfringens were 17%, 9.6%, and 8.7% in samples without spices, respectively; and 23.5%, 23.7%, and 29.6% in samples with spices, respectively. Two pathogenic genes, LT and EAST, were identified separately in four strains of E. coli. Salmonella and L. monocytogenes were isolated in 2.8% and 3.2% of the total samples, respectively.
Journal of environmental health 12/2008; 71(4):51-5, 63. · 1.01 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The present paper describes a microbiological method for the detection of antibacterial substances in poulry muscle. The method is based on the growth inhibition of Bacillus subtilis on an agar medium at a pH of 6.0, 7.2, and 8.0, Bacillus cereus on an agar medium at a pH of 6.0, M. luteus on agar medium at a pH of 6.0 and 8.0, and Escherichia coli on agar medium at a pH of 7.2, and on the use of confirmatory solutions (Pase, Paba, MgSO4) for the identification. The proposed method detects the main antibiotic groups: β- lactams, tetracyclines, aminoglycosides, macrolides, and quinolones at low levels. The results are obtained in 18-24 h. This technique can be used as a screening method for the detection of antibiotics in animal tissue, but a more specific method would be necessary for full identification of antimicrobials in screening positive samples.