Ming Cai

Tongji University, Shanghai, Shanghai Shi, China

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Publications (23)40.46 Total impact

  • Yong Yang · Pengfei Zan · Jinpeng Gong · Ming Cai
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    ABSTRACT: For the present study, the authors hypothesized that the d-dimer levels would be systematically raised in a postoperative population of patients younger than 50 with lower limb fractures and to define a feasible cutoff value for identification of venous thromboembolism (VTE). Doppler ultrasonography of lower limbs was performed pre- and postoperatively to evaluate for deep vein thrombosis in 150 patients who underwent open reduction and internal fixation (ORIF). Plasma d-dimer levels were assessed 2 days before surgery and on the 3rd, 7th, and 10th days after surgery. Statistical analysis was carried out to define a feasible threshold for the d-dimer levels. Plasma d-dimer levels were found to be systematically raised postoperatively, and they differed between patients with and without VTE significantly. On the third day after surgery, d-dimer levels of more than 3 mg/L indicated VTE with a sensitivity of 88.37% and a specificity of 96.96%, allowing for the definition of a feasible cutoff value. Duration of surgery, duration of tourniquet, ventilation time, and time of postoperative immobility of lower limbs were identified as highly significant risk factors for the development of VTE. Using a threshold of 3 mg/L, the d-dimer levels will screen out VTE with a high degree of sensitivity and specificity in younger patients who have undergone ORIF for lower limb fractures. © The Author(s) 2015.
    Clinical and Applied Thrombosis/Hemostasis 06/2015; DOI:10.1177/1076029615588784 · 1.58 Impact Factor
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    ABSTRACT: Intramedullary and extramedullary fixation methods are used in the management of subtrochanteric femur fractures. However, whether intramedullary or extramedullary fixation is the primary treatment for subtrochanteric femur fractures in adults remains debatable. Meta-analyses of prospective studies, level I. The Cochrane library, Embase, Google Scholar, and PubMed databases were searched separately for all relevant studies published before January 1, 2015. No language restriction was applied. Prospective randomized controlled trials that compared intramedullary or extramedullary internal fixation to repair subtrochanteric femur fractures in adults were included. We determined intraoperative data, postoperative complications, fracture fixation complications, wound infection, hospital stay days, and final outcome measures to assess the relative effects of different internal fixation methods for the treatment of subtrochanteric femur fractures in adults. Six studies were included in our meta-analysis. The relative risks (RRs) of revision rate was 83% lower (RR, 0.17, 95% confidence interval [CI], 0.05 to 0.60; P=0.006), fixation failure rate was 64% lower (RR, 0.36, 95% CI, 0.12 to 1.08; P=0.07), non-union rate was 77% lower (RR, 0.23, 95% CI, 0.07 to 0.81; P=0.02) in the intramedullary group compared with the extramedullary group. No significant differences were found between the intramedullary group and extramedullary group for intraoperative data, postoperative complications, wound infection, hospital stay days or final outcome measures. In conclusion, our meta-analysis suggests that there was no significant difference in intraoperative data, postoperative complications, wound infection, hospital stay days or final outcome measures between intramedullary and extramedullary internal fixation. However, a significant decrease occurred in the rate of fracture fixation complications for patients treated with intramedullary internal fixation, especially in elderly patients. Some differences were not significant, but the treatment of elderly subtrochanteric femur fractures using intramedullary internal fixation is recommended.
    Clinical Interventions in Aging 04/2015; 10:803-11. DOI:10.2147/CIA.S82119 · 1.82 Impact Factor
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    Kuan Liu · Pengcheng Liu · Run Liu · Xing Wu · Ming Cai
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    ABSTRACT: The association between leptin and bone mineral density (BMD) is controversial because of conflicting findings from previous studies. This meta-analysis aimed to provide an overview of the serum leptin levels and BMD in a healthy population. We reviewed the PubMed, Embase, and Cochrane Library databases until July 2014 for research on the association between leptin levels and BMD in healthy people. We included and analyzed 45 studies in this systematic review and meta-analysis. The pooled correlations between leptin and BMD were analyzed by using the method of the inverse of the variance. Leptin was positively associated with BMD and the bone mineral content (BMC), especially in postmenopausal women (pooled r: 0.13-0.49). Overall, high serum leptin levels were associated with higher BMD levels. This meta-analysis suggests that serum leptin levels are positively associated with BMD and BMC, especially in postmenopausal women. Copyright © 2015. Published by Elsevier B.V.
    Clinica Chimica Acta 03/2015; 444. DOI:10.1016/j.cca.2015.02.040 · 2.82 Impact Factor
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    ABSTRACT: Background The aim of this study was to compare radial head prosthesis replacement with open reduction and internal fixation (ORIF) in the surgical treatment of Mason type III radial head fractures in 72 elderly patients. Material/Methods Seventy-two elderly patients (mean age, 67.1±1.25 years, range, 62–81 years) with Mason type III radial head fractures were treated from January 2001 to June 2012. Of these, 37 cases received radial prosthesis and 35 cases were treated with ORIF. All patients were followed up for 10 to 15.6 months. Results Based on the elbow functional evaluation criteria score by Broberg and Morrey, 29 cases achieved excellent results, 7 were good, and 1 was fair in the replacement group. In the ORIF group, excellent results were seen in 24 cases, good in 9, and fair in 2. The rates of good or excellent results were 78.4% and 68.6% for prosthesis replacement patients and ORIF patients, respectively (P<0.05). The Visual Analogue Scores (VAS) for replacement and ORIF groups were 2.25 and 1.67, respectively (P<0.05). Conclusions The radial head prosthesis replacement method is a relatively better surgical approach than ORIF in the treatment of elderly patients with Mason type III radial head fractures.
    Medical science monitor: international medical journal of experimental and clinical research 01/2015; 21:90-3. DOI:10.12659/MSM.893048 · 1.22 Impact Factor
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    Xing Wu · Kuan Liu · Peng-Cheng Liu · Run Liu · Ming Cai
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    ABSTRACT: To investigate the effectiveness and safety of epidural steroid injections in patients with lumbar spinal stenosis (LSS). We performed a search on the CENTRAL, Pubmed, Embase and Cochrane databases up to September 2014. We recovered 17 original articles, of which only 10 were in full compliance with the randomized controlled trial (RCT) criteria. These articles were reviewed in an independent and blinded way by two reviewers who were previously trained to extract data and score their quality by the criteria of the Cochrane Handbook (5.1.0). We accepted ten studies with 1,010 participants. There is minimal evidence that shows that epidural steroid injections are better than lidocaine alone, regardless of the mode of epidural injection. There is a fair short-term and long-term benefit for treating spinal stenosis with local anesthetic and steroids. This meta-analysis suggests that epidural steroid injections provide limited improvement in short-term and long-term benefits in LSS patients.
    Drug Design, Development and Therapy 01/2015; 9:707. DOI:10.2147/DDDT.S78070 · 3.03 Impact Factor
  • Xinyu Cai · Ming Cai · Lieming Lou
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    ABSTRACT: Tendinopathy is a critical clinical problem as it is often asymptomatic at onset and during development, and is only recognized upon rupture of the tendon. It is common among recreational and competitive athletes. The present study sought to examine the molecular mechanism of the progression of tendinopathy by screening out differentially expressed genes (DEGs) and investigating their functions. In addition, the present study aimed to identify the small molecules, which exhibit potential effects, which could be utilized for the treatment of tendinopathy. The gene expression profile of tendinopathy, GSE26051 was downloaded from the Gene Expression Omnibus database, which included 23 control samples and 18 samples of tendinopathy. The DEGs were identified using the Limma package in the R programming language, and gene ontology and pathway enrichment analysis were performed. In addition, the potential regulatory microRNAs and the target sites of the transcription factors were screened out based on the molecular signature database. In addition, the DEGs were mapped to the connectivity map database to identify the potential small molecule drugs. A total of 318 genes were filtered as DEGs between diseased samples and normal control tendons. Additionally, genes, including laminin, α4, platelet‑derived growth factor α, laminin γ1 and Src homology 2 transforming protein 1 may induce tendinopathy through the focal adhesion pathway. Furthermore, the transcription factor, lymphoid enhancer‑binding factor 1 and its target genes, pantothenate kinase 2 and G protein‑coupled receptor kinase 5 were identified. The most significant microRNA, miR‑499, was screened and was found to regulate specific genes, including CUGBP2 and MYB. Additionally, the small molecules, Prestwick‑1082 and viomycin were identified to have the potential to repair disordered metabolic pathways and furthermore to remedy tendinopathy. The results of the present study assessed the mechanism of tendinopathy and screened small molecule drugs as potential treatments for this condition. In addition, the present findings have the potential for use in a clinical setting for the treatment of tendinopathy in the future.
    Molecular Medicine Reports 12/2014; 11(4). DOI:10.3892/mmr.2014.3081 · 1.48 Impact Factor
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    Peng-Cheng Liu · Hui Shi · Ming Cai
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    ABSTRACT: Epidural steroid injections (ESI) are widely used in patients with lumbar spinal stenosis (LSS), but data from RCTs to assess their effectiveness and safety are lacking. In 2012 a systematic review by Manchikanti et al. found it is fair for radiculitis secondary to LSS with lidocaine and steroids. Also Manchikanti et al. performed a 2-year follow-up RCT and they found ESI may be considered as an effective treatment for a select group of patients who have chronic function-limiting low back and lower extremity pain secondary to LSS. But in 2014 Friedly et al. reported that ESI offered minimal or no short-term benefit comparing with epidural lidocaine injections in managing LSS. All these RCTs make it harder for clinicians to translate the findings into treatment decisions. Manchikanti et al. also thought that RCT of ESI for LSS published in the NEJM leads to further confusion without clarification. Consequently, it is interesting Andersson hold the opinion that he will remain cautious in prescribing ESI for patients with LSS for the reason that the current best available data have not provided support for a clinically significant long-term benefit overall and that complications are possible. So we performed a search on CENTRAL, Pubmed, Embase and Cochrane databases up to October 25, 2014 to investigate the effectiveness and safety of ESI in patients with LSS. Therefore, 10 RCTs matched our selection criteria and were suitable for meta-analysis. Minimal or no significant difference was found between the ESI and the epidural lidocaine injection for the short-term benefit, such as change from leg pain Visual Analogue Scale (VAS) score; change from back pain VAS score. For the long-term benefit there was significant difference between the ESI and the epidural lidocaine injection for change from bodily pain (BP) and physical function (PF) subscale scores. With respect to epidural lidocaine injection, there was no significant difference for most outcome measures, such as change in Oswestry Disability Index (ODI) score; changes from weight (lbs); change in Opioid Intake (Morphine Equivalence mg). On the basis of the reviewed trials, when compared with lidocaine, we found no evidence to LSS therapy provide a statistically significant improvement in pain symptoms or walking ability in LSS patients. Further better and rigorous studies with long-term observation are required to elucidate the effectiveness of epidural steroid injection treatment for LSS.
    The 9th International Congress of Chinese Orthopaedic Associate; 11/2014
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    ABSTRACT: PurposeThe electron beam melting (EBM) Ti-6Al-4 V material technology has been developed over a short time period. It was introduced through a research to develop Ti-6Al-4 V implants for patients, but EBM printed locking compression plates have not been used for clinical implants. The main purpose of this study is to find whether the EBM Ti-6Al-4 V plate suit for clinical implants.Methods First, we scanned an AO-locking compression plate (LCP) and printed LCP samples using EBM. Next, we evaluated the EBM plate surface roughness through optical microscopy as well as the LCP and EBM plates¿ mechanical characteristics using the ASTM standard, which is commonly used to test the mechanical properties of bone plates subject to bending. Each sample was examined using a single-cycle four-point bending test and hardness testing to acquire data on bending stiffness, bending strength, bending structural stiffness, and hardness.ResultsThe results show significant differences in bending stiffness, bending strength, bending structural stiffness, and hardness between the samples using EBM and the original LCP plates. The EBM-printed samples¿ surface roughness was 0.49¿±¿0.02 ¿m. The mean hardness of the LCP sample was 266.67 HV10¿±¿5.8, and the EBM-printed sample mean hardness was 341.1 HV10¿±¿1.93. The EBM samples¿ bending stiffness was 87.67%, which is greater than using the LCP plates¿; and the bending strength was 190.7% greater, the bending structural stiffness was 73.2% greater, and the hardness was 27.9% greater.Conclusions The results show that the EBM plates¿ general mechanical strength was significantly greater than the LCP plates. An EBM plate is advantageous for clinical implants because it can be customized with great potential for improvement.
    Journal of Orthopaedic Surgery and Research 11/2014; 9(1):106. DOI:10.1186/s13018-014-0106-3 · 1.58 Impact Factor
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    ABSTRACT: Previous studies have shown conflicting results between the association of leptin receptor (LEPR) genetic polymorphisms and cancer risk. The frequent LEPR Lys656Asn or Ser343Ser genetic polymorphism has been demonstrated to be functional and may promote genetic susceptibility to cancers. However, the association between the LEPR Lys656Asn or Ser343Ser genetic polymorphism and cancer risk remains to be determined. To improve the understanding of the LEPR Lys656Asn or Ser343Ser genetic polymorphism role in global cancer, a comprehensive meta-analysis was conducted that comprised 2,480 cases and 3,162 controls. The LEPR Lys656Asn or Ser343Ser genetic polymorphism did not significantly affect the cancer risk. In the stratified analysis, there was no significant association of the LEPR Lys656Asn or Ser343Ser variants with any type of cancer under any model. In addition, significantly increased risks were found in the Asian population in heterozygous codominant [odds ratio (OR), 1.24 (1.01-1.53)] and dominant [OR, 1.24 (1.02-1.50)] genetic models. A significantly increased susceptibility to cancer was not found when stratified by study design. There were no significant differences found in genotype method and sample size in cases among the genotypes. These findings indicated a lack of association between LEPR Lys656Asn or Ser343Ser polymorphisms and cancer susceptibility, however, these polymorphisms may increase the cancer susceptibility among the Asian population, particularly in the dominant genetic model. The single-nucleotide polymorphism is also suggested to function as a dominant mutation, which requires verification or association with functional studies.
    11/2014; 2(6):849-854. DOI:10.3892/br.2014.326
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    ABSTRACT: Purpose: The results from the published studies on the as-sociation between LEP (leptin) genetic polymorphism and cancer risk are conflicting. The common G2548A genetic polymorphism has been reported to be functional and may contribute to genetic susceptibility to cancers. However, the association between LEP G2548A genetic polymor-phism and cancer risk remains inconclusive. Methods: To better understand the role of LEP G2548A genetic polymorphism in global cancer, we conducted this comprehensive meta-analysis encompassing 6860 cases and 7956 controls. Results: Overall, the LEP G2548A genetic polymorphism was associated with higher cancer risk in three genetic models (AA vs GG, AA vs AG/GG, A vs G). In the strat-ified analysis, there was significant association of LEP G2548A variant with non-Hodgkin's lymphoma (NHL) under homozygous co-dominant model (OR=1.27, 95% CI 1.01-1.60) and additive genetic model (OR=1.14, 95% CI 1.01-1.28). Moreover, a significantly increased cancer risk was found in three genetic models (AA vs GG, AA vs AG/GG, A vs G) among Caucasian population. For Asians, no significant associations were observed in any genetic model tested. Conclusions: These findings suggest that the LEP G2548A genetic polymorphism may increase the suscep-tibility of cancers in NHL, especially in the homozygote co-dominant model and the additive genetic model among Caucasian populations. The phenomenon also indicates that the SNP functions as a recessive mutation, which needs to be verified or linked with functional studies.
    Journal of B.U.ON.: official journal of the Balkan Union of Oncology 10/2014; 19(4):1096-1104. · 0.71 Impact Factor
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    ABSTRACT: Purpose: The results from the published studies on the association between LEPR genetic polymorphisms and cancer risk are conflicting. The common LEPR Q223R genetic polymorphism has been reported to be functional and may contribute to genetic susceptibility to cancer. However, the association between LEPR Q223R genetic polymorphism and cancer risk remains inconclusive. Methods: To better understand the role of LEPR Q223R genetic polymorphism in global cancer, we conducted this comprehensive meta-analysis encompassing 9139 cases and 11282 controls. Results: Overall, the LEPR Q223R genetic polymorphism did not significantly affect the risk of cancer. In the stratified analysis, there was no significant association of LEPR Q223R variant with breast cancer, colorectal cancer and non-Hodgkin's lymphoma (NHL) under any models. Moreover, significantly increased risks were found in Asian and African in all genetic models tested. When stratified by study design, no significantly increased susceptibility to cancer was found among any studies. No significantly differences in sample size in cases were found among genotypes. Conclusions: These findings suggested lack of association between LEPR Q223R polymorphisms and cancer susceptibility, but the LEPR Q223R genetic polymorphism may increase the susceptibility to cancers in Asian and African individuals. Large, well designed epidemiological studies are needed to validate our findings.
    Journal of B.U.ON.: official journal of the Balkan Union of Oncology 07/2014; 19(3):855-862. · 0.71 Impact Factor
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    ABSTRACT: Osteosarcoma (OS) is the most common primary malignant tumor of bone in children and adolescents. However, few biomarkers of diagnostic significance have been established. In recent years, high-throughput transcriptomic and metabolomic approaches make it possible for studying the levels of thousands of biomarkers simultaneously. In this study, we integrated two disparate transcriptomic and metabolomic datasets to find meaningful biomarkers and then used an independent dataset to test the sensibility and specificity of these biomarkers. By using integrated two datasets, we discovered that the biomarkers involved in the glycolysis pathway are highly enriched, including 4 genes (ENO1, TPI1, PKG1 and LDHC) and 2 metabolites (lactate and pyruvate). The 4 genes were significantly down-regulated in OS samples as well as the 2 metabolites. The mixed metabolites + genes signature also outperformed metabolites or genes alone, with recall being 0.813 and F-measure being 0.812. And the AUC value of metabolites + genes classifier was 0.825 (compared to 0.58 for metabolites and 0.821 for genes alone). Our findings establish that integrated transcriptomic and metabolomic signature can be used to distinguish OS malignant with good diagnostic accuracy superior to other methods.
    Journal of Cancer Research and Clinical Oncology 06/2014; 140(10). DOI:10.1007/s00432-014-1719-y · 3.01 Impact Factor
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    ABSTRACT: The results from the published studies on the association between leptin (LEP) genetic polymorphism and cancer risk are conflicting. The common A19G (rs2167270) genetic polymorphism has been reported to be functional and may contribute to genetic susceptibility to cancers. However, the association between LEP A19G (rs2167270) genetic polymorphism and cancer risk remains inconclusive. To better understand the role of LEP A19G (rs2167270) genetic polymorphism in global cancer, we conducted this comprehensive meta-analysis encompassing 5,679 cases and 7,443 controls. Overall, the LEP A19G (rs2167270) genetic polymorphism was associated with lower cancer risk. In the stratified analysis, significant associations were found between the LEP A19G (rs2167270) genetic polymorphism and colorectal cancer and non-Hodgkin's lymphoma. For colorectal cancer, there was no significant association of LEP A19G (rs2167270) variant with this disease under heterozygous codominant model [odds ratio (OR) = 1.11 (0.97-1.27)], dominant genetic model [OR = 1.03 (0.91-1.17)], and additive genetic model [OR = 0.94 (0.86-1.03)]; however, there was a marginal association under homozygous codominant model [OR = 0.80 (0.66-0.97)] and recessive genetic model [OR = 0.75 (0.63-0.90)]. For non-Hodgkin's lymphoma, there was a significant association of LEP A19G (rs2167270) variant with the disease under homozygous codominant model [OR = 0.74 (0.59-0.94)], recessive genetic model [OR = 0.76 (0.61-0.94)], and additive genetic model [OR = 0.89 (0.80-0.99)], but not under heterozygous codominant model [OR = 0.95 (0.82-1.10)] and dominant genetic model [OR = 0.91 (0.79-1.04)]. Moreover, a significantly decreased cancer risk was found in recessive genetic model among Latin American population. When stratified by study design, significantly elevated susceptibility to cancer was not found among any studies. No significantly differences in genotype method and sample size in cases were found among genotypes. These findings suggest that the LEP A19G (rs2167270) genetic polymorphism may decrease the susceptibility to cancers in colorectal cancer and non-Hodgkin's lymphoma, when assuming a homozygote codominant model and a recessive genetic model among Latin American population. The phenomenon also indicates that the SNP functions as a recessive mutation, which needs to be verified or linked with functional studies.
    Tumor Biology 05/2014; 35(8). DOI:10.1007/s13277-014-2088-5 · 3.61 Impact Factor
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    ABSTRACT: Previous studies have shown that the rate of breast cancer metastasis correlates with the expression of vacuolar H+-ATPases (V-ATPases). However, how V-ATPase is involved in breast cancer metastasis remains unknown. Our previous study showed that Atp6v1c1-depleted osteoclasts did not form organized actin rings and that Atp6v1c1 co-localizes with F-actin. In this study, we found that the normal arrangement of filamentous actin is disrupted in Atp6v1c1-depleted 4T1 mouse breast cancer cells and in the ATP6V1C1-depleted human breast cancer cell lines MDA-MB-231 and MDA-MB-435s. We further found that Atp6v1c1 co-localizes with F-actin in 4T1 cells. The results of our study suggest that high expression of Atp6v1c1 affects the actin structure of cancer cells such that it facilitates breast cancer metastasis. The findings also indicate that Atp6v1c1 could be a novel target for breast cancer metastasis therapy.
    PLoS ONE 01/2014; 9(1). DOI:10.1371/annotation/ca9f2d5b-93ab-4d7c-b577-cd045b343e53 · 3.53 Impact Factor
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    P Liu · H Shi · Y Yang · R Liu · C Huang · H Shu · J Li · Q Chen · M Cai
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    ABSTRACT: The results from the published studies on the association between hypoxia-inducible factor-1(Hif-1/HIF-1) polymorphisms and cancer risk are conflicting. The common 1790G/A rs11549467) genetic polymorphism has been reported to be functional and may contribute to genetic susceptibility to cancers. However, the association between 1790G/A (rs11549467) and cancer risk remains inconclusive.To better understand the role of 1790G/A (rs11549467) polymorphism in cancer, we conducted this comprehensive meta-analysis encompassing 6337 cases and 9302 controls.Overall, the 1790G/A (rs11549467) genetic polymorphism was associated with higher cancer risk. In the stratified analysis, significant associations were found between the Hif-1/HIF-1 1790G/A polymorphism and lung cancer, pancreatic cancer and oral squamous cell carcinoma. We also observed that the AA genotype might modulate lung cancer (OR=5.42[2.75-10.70]), pancreatic cancer (OR=9.30[1.12-77.61]) and oral squamous cell carcinoma (OSCC) (OR=13.32[1.57-112.75]) risk comparing with the GG genotype. Moreover, a significantly increased cancer risk was found in homozygote comparison (AA vs. GG) and recessive genetic model (AA vs. AG/GG) among Caucasian population. When stratified by study design, significantly elevated susceptibility to cancer was found among hospital-based studies.These findings suggested that the 1790G/A (rs11549467) genetic polymorphism may contribute to the susceptibility of cancers except gynecologic cancer, especially in homozygote comparison and recessive genetic model among Caucasian population, and this SNP was significantly associated with the lung cancer, pancreatic cancer and oral squamous cell carcinoma (OSCC). The phenomenon also indicates that the SNP functions as a recessive mutation needs to be verified or linked with functional studies. Keywords: Hif-1/HIF-1, 1790G/A, cancer, genetic polymorphism, meta-analysis.
    Neoplasma 01/2014; 61(3):340-51. DOI:10.4149/neo_2014_044 · 1.64 Impact Factor
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    ABSTRACT: There has been a strong interest in searching for natural therapies for osteoporosis. Epimedium koreanum Nakai is an herb that is commonly used in East Asia to treat osteoporosis, and most studies of its activity have focused on its major ingredient, icariin. In this study, maohuoside A (MHA), a single compound isolated from the E. koreanum, was found to promote osteogenesis in mouse bone marrow-derived mesenchymal stem cells. We hypothesise that, if MHA potently induces osteogenic differentiation in a bone morphogenetic protein-dependent manner, it may be used to broaden the sources for cell transplantation and thereby establish more efficient bone regeneration systems. Real-time polymerase chain reaction and western blot were used to detect the expression of SMAD4, a marker of bone formation. Microcomputed tomography and histomorphometric techniques showed that oral treatment with MHA was followed by an increase in the bone mineral density of the lumbar vertebrae in mice. This result also indicates that MHA may directly activate osteopontin gene transcription. In conclusion, MHA seems to enhance the osteogenesis of bone marrow-derived mesenchymal stem cells at least partly via bone morphogenetic protein signalling. Copyright © 2012 John Wiley & Sons, Ltd.
    Phytotherapy Research 08/2013; 27(8). DOI:10.1002/ptr.4840 · 2.40 Impact Factor
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    ABSTRACT: Background/Aims: Over the past decade, heat shock protein 90 (Hsp90) has emerged as a potential therapeutic target for cancer. However, the molecular mechanisms of down-regulation Hsp90 expression in osteosarcoma are incompletely understood. To develop potential therapy targeting Heat shock protein 90B1 (Hsp90B1), we studied the roles of miR- 223 in the proliferation and apoptosis of human osteosarcoma. Methods: pcDNA3.1(+)- miR-223 plasmid vectors were constructed and transfected into MG63 cells. Co-transfection of miR-223 expression vector with pMIR-Hsp90B1 (The recombined vector of pMIR-GLOTM luciferase vector containing Hsp90B1-3'UTR) led to the reduced activity of luciferase in a dual-luciferase reporter gene assay, suggesting that Hsp90B1 is a target gene of miR-223. Expression of HSP90B1 was detected by RT-PCR and western blotting analysis. Cell proliferation was determined using the MTT assay. Cell-cycle distribution and apoptosis were examined by flow cytometry. PI3K, p-Akt, Akt, mTOR, Bcl-2 and Bid were also detected by western blotting analysis. After a mouse xenograft model of human MG63 tumors was constructed, tumor growth, microvessel density and proliferation in each group was determined. Results: The pcDNA3.1(+)-miR-223 vector efficiently suppressed the expression of HSP90B1, while silencing miR-223 increased expression of Hsp90B1. Furthermore, overexpression of miR-223 results in significant inhibition of cell growth on culture plates. Moreover, cancer cells showed significant G0/G1 arrest and increased apoptosis due to gene silencing. Protein levels of PI3k, p-Akt, mTOR, and Bcl-2 were decreased, whereas Bid levels were increased. Microvessel density as assessed by CD34 levels and cell growth by PCNA levels decreased according to immunohistochemical analysis. Conclusion: Hsp90B1 is a direct target of miR-223 and miR- 223 may have a tumor suppressor function in osteosarcoma through the PI3K/Akt/mTOR pathway and could be used in anticancer therapies in osteosarcoma.
    Cellular Physiology and Biochemistry 12/2012; 30(6):1481-1490. DOI:10.1159/000343336 · 3.55 Impact Factor
  • Ming Cai · Kun Tao · Chunxi Yang · Shaohua Li
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    ABSTRACT: The purpose of this single-center, randomized, controlled trial was to report on the 2-year outcomes of proximal humerus fractures in elderly patients treated with open reduction and internal fixation (ORIF) with either a locking plate or shoulder hemiarthroplasty.Thirty-two patients (87% women) with a mean age of 71.9 years (range, 67-86 years) were treated with ORIF with either a locking plate or shoulder hemiarthroplasty after shoulder injury. The main outcome measures were the Constant score, Disabilities of the Arm, Shoulder and Hand (DASH) score, and health-related quality of life (HRQoL) according to the EQ-5D (EuroQol Group, Rotterdam, The Netherlands). At final 2-year follow-up, DASH and pain scores favored the shoulder hemiarthroplasty group. Mean flexion was 129° in the shoulder hemiarthroplasty group and 117° in the ORIF group (P=.27), and mean abduction was 123° in the shoulder hemiarthroplasty group and 111° in the ORIF group (P=.41). In the shoulder hemiarthroplasty group, the EQ-5D index score decreased from 0.85±0.21 before injury to 0.65±0.14 at 4 months postoperatively. The score was 0.79±0.24 at 12 months postoperatively and 0.81±0.17 at 24 months postoperatively.The results of this study indicate an advantage in functional outcomes and HRQoL favoring shoulder hemiarthroplasty compared with ORIF with a locking plate, although most outcomes were not significantly different.
    Orthopedics 09/2012; 35(9):e1340-6. DOI:10.3928/01477447-20120822-19 · 0.98 Impact Factor
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    ABSTRACT: INTRODUCTION: Correct rotational alignment of the tibial component is crucial for total knee arthroplasty (TKA). Several studies have indicated that the best rotational orientation of the tibial component is close to the medial border of the tibial tubercle. However, it remains obscure whether it is always right. Thus, the objective of current study was to quantify tibial rotational alignment in 120 primary rotating platform TKAs using the medial border of tibial tubercle as a landmark between July 2008 and June 2010. METHODS: The femoral component was positioned parallel to the transepicondlylar axis, and a rotating platform trial insert was used to determine tibial insert rotational alignment relative to the most medial aspect of the tibial tubercle with the knee in full extension. Rotational alignment of the components was detected based on radiograph and CT scan. This investigation is based on the premise that all neutral points would lie within 10° of the mean. RESULTS: The mean divergence external to the medial border of the tubercle was 2.3°±3.5°. However, six of the knees (5%) had neutral points ≥10° from the mean, including two valgus knees measured 10° of internal rotation and four varus knees measured 10° of external rotation. CONCLUSION: Using the medial border of tibial tubercle as a landmark does not always result in a good femoro-tibial rotational alignment. Surgeons using fixed bearings component should be aware of this effect to avoid suboptimal outcomes resulting from tibiofemoral rotational malalignment in full extension. LEVEL OF EVIDENCE: Diagnostic study, Level II-3.
    The Knee 07/2012; 21(1). DOI:10.1016/j.knee.2012.06.007 · 1.70 Impact Factor
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    ABSTRACT: TAK-778, a derivative of ipriflavone, has been shown to induce bone growth both in vitro and in vivo. Recently, it has been shown that TAK-778 can enhance osteoblast differentiation of human bone marrow cells via an estrogen receptor (ER)-dependent pathway. However, the mechanism by which TAK-778 exerts its effect in vivo has not been determined. Considering the evidence that TAK-778 acts via ER-mediated signaling in vitro, in the present study we tested if TAK-778 induced osteogenesis via an ER-dependent pathway using an ovariectomized (OVX) rat model. Two weeks after test animals underwent ovariectomy, TAK-778 and/or tamoxifen was administered orally over 3 months. Vehicle-treated and sham-operated rats served as controls. The bone mineral density (BMD) of the lumbar vertebrae and sagittal two-dimensional images of the L3 vertebral body were measured. In addition, bone formation rates (BFR) and serum calcium and osteocalcin levels were measured. The results indicated that TAK-778 significantly increased BMD, serum calcium and osteocalcin levels, and BFR when compared to that of the vehicle-treated group. However, tamoxifen, a well-known ER antagonist, clearly inhibited the increase in these parameters induced by TAK-778. In addition, micro-computed tomography scans showed that treatment with TAK-778 increased the structure model index, bone volume/tissue volume, and trabecular thickness parameters and decreased the trabecular separation/spacing in OVX rats. Tamoxifen suppressed these effects when administered in combination with TAK-778. Taken together, the present study showed that TAK-778 enhanced bone formation in OVX rats and that this effect was dependent on an ER-mediated pathway.
    Journal of Bone and Mineral Metabolism 03/2011; 29(2):168-73. DOI:10.1007/s00774-010-0208-x · 2.11 Impact Factor

Publication Stats

42 Citations
40.46 Total Impact Points

Institutions

  • 2012–2015
    • Tongji University
      • Medical School
      Shanghai, Shanghai Shi, China
  • 2008–2009
    • Shanghai Jiao Tong University
      • Department of Orthopaedics
      Shanghai, Shanghai Shi, China