Yuming Luo

Capital Medical University, Peping, Beijing, China

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Publications (17)33.75 Total impact

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    ABSTRACT: Objectives: We investigated the effect of a microcatheter-based selectively induced intra-arterial hypothermia on hemodynamic changes following transient cerebral ischemia in rats. Methods: Stroke was induced in male Sprague-Dawley rats by a two-hour middle cerebral artery occlusion (MCAO) using a microcatheter. After the two-hour MCAO, 0·9% cold saline (0°C) was selectively infused through a microcatheter. Cerebral blood flow (CBF) in the ischemic brain region was continuously monitored by Laser-Doppler flowmetry (LDF) during the procedure. Following ischemia/reperfusion, serial functional neurologic testing was performed, and cerebral infarct volume was evaluated after 48 hours. Results: The local cold saline infusion, via a microcatheter, achieved a rapid induction of brain hypothermia (cerebral cortex from 37·1 ± 0·3 to 30·7 ± 0·4°C; striatum from 37·5 ± 0·3 to 30·9 ± 0·5°C). When compared to the non-treatment group, the local cold saline infusion treatment reduced both post-ischemic hyperperfusion (about 40%, P < 0·01) and delayed post-ischemic hypoperfusion (P < 0·01), improved functional neurological testing (P < 0·01), and reduced both cerebral infarction volume (40·6 ± 5·3 vs. 61·7 ± 8·6%, P < 0·01) and cerebral edema (7·8 ± 2·6 vs.15·4 ± 3·2%, P < 0·01). Conclusion: Cold saline, when infused directly into the ischemic brain region, can confer robust neuroprotection by reducing immediate post-ischemic hyperperfusion and delayed post-ischemic hypoperfusion.
    Neurological Research 10/2014; 37(3):1743132814Y0000000451. DOI:10.1179/1743132814Y.0000000451 · 1.45 Impact Factor
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    ABSTRACT: The ABC1 atypical kinases (aPKs) have been extensively studied in bacteria, yeast and human, where their mutation causes a deficiency of ubiquinone, an isoprenoid compound in the respiratory electron transfer chain. Considerably less is known, however, about their evolution and function in higher plants. In this study, we identified 16 ABC1-domain containing genes from model plant rice by comprehensive genome analysis and gene cloning. Detection of positive selection showed that purifying selection was the major force underlying the evolution of most rice and Arabidopsis ABC1s. However, the expression profiles of plant ABC1s appear to have diverged based on microarray data analysis and realtime PCR; some of them are primarily expressed in developing leaves, some in stamens and/or mature pollen, whereas others in diverse tissues and organs. The possible functions of plant ABC1s were identified using genome-wide coexpression analysis. This analysis suggested that many of them might be involved in the regulation of isoprenoid biosynthesis. In conclusion, the plant ABC1 aPKs, which harbor distinct expression patterns, might have conserved functions in modulating isoprenoid metabolism.
    Journal of Plant Biochemistry and Biotechnology 04/2014; 24(2). DOI:10.1007/s13562-014-0259-5 · 0.81 Impact Factor
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    ABSTRACT: Stroke is deemed a worldwide leading cause of neurological disability and death, however, there is currently no promising pharmacotherapy for acute ischemic stroke aside from intravenous or intra-arterial thrombolysis. Yet because of the narrow therapeutic time window involved, thrombolytic application is very restricted in clinical settings. Accumulating data suggest that non-pharmaceutical therapies for stroke might provide new opportunities for stroke treatment. Here we review recent research progress in the mechanisms and clinical implications of non-pharmaceutical therapies, mainly including neuroprotective approaches such as hypothermia, ischemic/hypoxic conditioning, acupuncture, medical gases, transcranial laser therapy, etc. In addition, we briefly summarize mechanical endovascular recanalization devices and recovery devices for the treatment of the chronic phase of stroke and discuss the relative merits of these devices.
    Progress in Neurobiology 01/2014; 115. DOI:10.1016/j.pneurobio.2013.12.007 · 10.30 Impact Factor
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    ABSTRACT: Acidic soils inhibit crop yield and reduce grain quality. One of the major contributing factors to acidic soil is the presence of soluble aluminum (Al(3+)) ions, but the mechanisms underlying plant responses to Al(3+) toxicity remain elusive. Nitric oxide (NO) is an important messenger and participates in various plant physiological responses. Here, we demonstrate that Al(3+) induced an increase of NO in rice seedlings; adding exogenous NO alleviated the Al(3+) toxicity related to rice growth and photosynthetic capacity, effects which could be reversed by suppressing NO metabolism. Comparative proteomic analyses successfully identified 92 proteins that showed differential expression after Al(3+) or NO treatment. In particular, some of the proteins are involved in reactive oxygen species (ROS) and reactive nitrogen species (RNS) metabolism. Further analyses confirmed that NO treatment reduced Al(3+)-induced ROS and RNS toxicities by increasing the activities and protein expression of antioxidant enzymes, as well as S-nitrosoglutathione reductase (GSNOR). Suppressing GSNOR enzymatic activity aggravated Al(3+) damage to rice and increased the accumulation of RNS. NO treatment altered the expression of proteins associated with cell wall synthesis, cell division and cell structure, calcium signaling and defense responses. Based on these results, we propose that NO activates multiple pathways that enhance rice adaptation to Al(3+) toxicity. Such findings may be applicable to crop engineering to enhance yield and improve stress tolerance.
    Journal of Proteome Research 01/2013; 12(3). DOI:10.1021/pr300971n · 5.00 Impact Factor
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    ABSTRACT: A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxytigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and ¹H-NMR.
    Molecules 12/2012; 17(8):9002-9. DOI:10.3390/molecules17089002 · 2.10 Impact Factor
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    ABSTRACT: Bitter gourd (Momordica charantia Linn.) is an important vegetable crop. We developed 11 polymorphic M. charantia microsatellite loci and examined the genetic diversity of 55 Chinese M. charantia germplasm cultivars at these loci. The average number of alleles per locus ranged from 3 to 8, the expected heterozygosity of each locus varied from 0.132 to 0.786 with an average of 0.621, and the maximum polymorphism information content value was 0.744 with an average of 0.572. These values suggested that the tested M. charantia germplasm has a relatively high genetic diversity at the 11 microsatellite loci. Based on transferability testing, some of these M. charantia microsatellite markers were transferable to eight other cucurbit species. Thus the development of these markers has enriched the microsatellite marker bank of cucurbit species; they can be used as a powerful tool for studying the genetic diversity of the germplasm of M. charantia and other cucurbit species.
    Scientia Horticulturae 06/2012; 140:115-118. DOI:10.1016/j.scienta.2012.03.024 · 1.50 Impact Factor
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    ABSTRACT: While recent studies suggest that remote ischemic postconditioning (RIP) therapy may be of benefit to patients with acute ischemic stroke, RIP's effects on intracerebral hemorrhage (ICH) still remains unclear. In the present study, the use of RIP in a rat model ICH was investigated to elucidate any potential beneficial or detrimental effects as determined by motor testing, blood brain barrier integrity, and brain water content, as well as aquaporin-4 (AQP-4) and matrix metalloproteinase-9 (MMP-9) expression. ICH was induced in Sprague-Dawley rats and they were randomized into either a control (n = 24) or RIP treatment (n = 24) group. RIP was performed by repetitive, brief occlusion and release of the bilateral femoral arteries. Functional outcome in each group was assessed by neurologic deficits on vibrissae-elicited forelimb placing test and a 12-point outcome scale. At 72 hours, brain blood volume, water content, blood-brain barrier (BBB) permeability, and protein expression of AQP-4 and MMP-9 were determined. This collagenase model yielded well-defined striatal hematomas. Vibrissae-elicited forelimb placement was significantly (P<0·01) affected by ICH. However, there was no significant difference between the RIP and control groups at either 24 or 72 hours. A 12-point neurological deficit score also failed to differentiate between the RIP and control. There were no significant differences between the two groups in cerebral blood volumes, brain water content, Evans blue extravasations, and expressions of AQP-4 and MMP-9. Although RIP did not show a beneficial effect in our ICH model, treatment with RIP did not exacerbate ICH.
    Neurological Research 01/2012; 34(2):143-8. DOI:10.1179/1743132811Y.0000000073 · 1.45 Impact Factor
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    ABSTRACT: The antioxidant activities of water extract (WE), ethanol extract (EE), residue water extract (RWE) and petroleum ether (PF), ethyl acetate (EF), n-BuOH (BF) and water (WF) fractions of the ethanol extract from Eupatorium Lindley DC were investigated for the first time. Total phenolics content, DPPH radical scavenging activities, superoxide radical scavenging activities, total reduction capability, and ferrous ions chelating activities were determined for all the extracts and fractions. The results showed that all the extracts and fractions exhibited antioxidant activities with different magnitudes of potency. Among all the samples, WE and RWE exhibited the best antioxidant capacities, the BF also exhibited high antioxidant abilities in all tests except for the metal chelating activity, while the other extracts and fractions were relatively weak antioxidants. The BF had the highest total phenolics contents in all extracts and fractions, and the WE and RWE were found to be rich in tannins. Furthermore, the content of total phenolics showed good correlation with DPPH radical scavenging activity, superoxide anion radical scavenging activity, and the reducing power. Phenolic composition of all the extracts and fractions was identified and quantified by HPLC. The results indicate that the extracts of E. Lindley DC might be a useful potential source of natural antioxidant ingredients.
    Molecules 12/2011; 16(7):5998-6009. DOI:10.3390/molecules16075998 · 2.10 Impact Factor
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    ABSTRACT: Proteomic approach combining two-dimensional gel electrophoresis (2-DE), mass spectrometry and bioinformatics analysis were applied to identify major proteins in extracts of mature wheat seeds. About 920 or 700 protein spots were detected on 2-DE gels by silver staining or colloidal Coomassie Brilliant Blue staining. Eighty spots with higher abundance were selected to cut for in-gel digestion followed by matrix-assisted laser desorption/ionization-mass spectrometry-peptide map fingerprint analysis and electrospray ionization mass spectrometry-peptide sequence tags analysis. Database searches using measured peptide masses or peptide sequence tags querying wheat expressed sequence tags determined protein identities of 73 spots. These identified proteins were categorized into six classes according to the functional annotation including those with unknown functions and difficult to classify. Proteins involved in storage proteins, metabolism, defense, chaperones and allergy were the major categories. The present identification of proteins in major spots from 2-D gels includes 11 different proteins from 29 spots from wheat seed extract. It is suggested that post-translational processing or isoforms causes the same proteins to occur in different spots. In addition, we also discussed the efficiency of protein identification using species-specific EST databases.
    African journal of agricultural research 03/2011; 6:808-816. · 0.26 Impact Factor
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    ABSTRACT: Proteomic approaches based on two-dimensional gel electrophoresis, mass spectrometry and database search are widely used to address questions about the development, physiology and quality of seeds. Identification of proteins is of great importance in proteomic analyses. For seed crops without full genome information, cross-species protein identification by mass spectrometry-driven sequence similarity search can be used. However, this approach is risky due to protein polymorphism between different species. Species-specific expressed sequence tag (EST) databases are an invaluable resource, which complements mass spectrometry data analysis for protein identification. Here, we illustrate a modified method of protein identification and characterization using species-specific EST databases and peptide mass fingerprinting with an example of protein identification. This method is reliable, supplements the existing methods, and improves the efficiency and accuracy of protein identification for seed crops for which complete genome information is not available.
    Seed Science Research 11/2010; 20(04):257 - 262. DOI:10.1017/S0960258510000243 · 1.85 Impact Factor
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    ABSTRACT: The interferon (IFN) was described as a substance secreted by virally infected cells and endowed with the ability to render cells capable of interfering with a subsequent viral infection. In eutherian mammals, type I IFNs comprises seven major homologous subgroups including IFNA, IFNB, IFND, IFNE, IFNK, IFNW and IFNX. They are the key cytokines orchestrating host antiviral defense and other physiological processes. All type I IFNs are clustered together in specific regions in humans, mice and bovine genomes. Two mammalian IFN genes of ancient origin, IFNB and IFNE, define the outer limits of the locus, with all the other genes, except IFNK, distributed between these two markers. Twenty four type I IFN genes were identified in the genome of horse (Equus caballus). They included eight IFNWs (two are pseudogenes), six IFNAs, three IFNBs and IFNXs, two IFNDs, one IFNK and IFNE. Interestingly, IFND, which has been discovered only in pigs to date, was also found in equine genome. However, IFNT, which was identified in bovine genome, did not exist in equine genome. The specific distribution of type I IFN in equine genome suggested these IFNs were required in immune defense against particular pathogens affecting horse itself. The equine type I IFN locus also had two sub-loci (1 & 2) distributed in different chromosomes. The larger sub-loci encompassing 530 kb was located on chromosomes 23. Another smaller sub-loci encompassing 86 kb was located on chromosomes Un0111. It seemed that equine type I IFN locus showed its specific property different from those of mouse, human, pig and bovine genome, which were located on the same chromosomes.
    African journal of agricultural research 10/2010; 5:2551-2555. · 0.26 Impact Factor
  • Liming Yang, Yuming Luo, Jifu Wei
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    ABSTRACT: Ikaros is a member of the Kruppel family of zinc finger DNA-binding proteins. The Ikaros protein contains two separate regions of zinc-finger domains: 4 DNA-binding zinc fingers near the N-terminus and 2 zinc fingers for protein-protein interactions near the C-terminus. Here, we identified the Ikaros gene from 14 vertebrate genomes and found Ikaros existed in all kinds of vertebrate including fish, amphibians, birds and mammals. Moreover, except rat and Xenopus tropicalis Ikaros proteins, which lack the first C2H2-type 1 Zinc finger region, all identified Ikaros proteins contain six C2H2-type 1 Zinc finger regions. We found human Ikaros gene showed a predominant expression in the liver, lymph node, thymus, intestine, lung, mammary gland, bone marrow, brain, heart, placenta and prostate. Moreover, four available SNPs disrupted an existing exonic splicing enhancer were identified in Ikaros. Besides the reported acute lymphoblastic leukemia (ALL), the expression of Ikaros was related to the prognosis of 13 cases of cancers including blood cancers, breast, lung, ovarian and skin cancer. Moreover, the relationship between the expression of Ikaros and prognosis varied in different cancers, even in the same cancer from different database. Two tumor-related transcriptional factor (c-Fos and Elk-1) binding sites were identified within the 1.5-kb regions upstream of the transcriptional start site of human Ikaros, which may be involved in the effect of Ikaros in tumors.
    Oncology Reports 08/2010; 24(2):571-7. · 2.19 Impact Factor
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    ABSTRACT: Interferon (IFN)-lambdas, including INF-lambda1, -lambda2, and -lambda3, are a newly described group of cytokines distantly related to the type I IFNs and IL-10 family members. IFN-lambda1, -lambda2, and -lambda3 bind to the same receptor (known as IL28RA) to exert their antiviral, antitumor and immunomodulatory effects. Here, we identified IL28RA genes from the genome of human, chimpanzee, macaque, orangutan, mouse, horse, rat, dog, chicken, and found that only one IL28RA existed in each genome. All the identified IL28RAs are single-pass type I membrane proteins except chicken IL28RA. They belong to the type II cytokine receptor family and contain one fibronectin type-III domain. We found human IL28RA was expressed in lymphs, testes, lymphoma, teratocarcinoma, pediatric pre-B cell acute lymphoblastic leukemia, germinal center B cells, embryonic stem cells, fetal lung, and also expressed in bladder, blood and breast cancers, glioma, head and neck cancer and lung cancer tissues. Three tumor-related transcriptional factor binding sites (AP-2, c-Jun and P53) were identified within the 1.0-kb regions upstream of the transcriptional start site of human IL28RA. Meta-analysis of the prognostic value of IL28RA genes in various cancers found that the expression of IL28RA was indeed related to the cancer prognosis in certain cancers. The STAT1 binding sites in the promoter region of IL28RA implied a specific mechanism for the amplifying effects of IFN-lambdas. The LyF-1 binding sites in the promoter region of IL28RA imply that IFN-lambdas were involved in the differentiation of early B and T cells.
    International Journal of Molecular Medicine 05/2010; 25(5):807-12. · 1.88 Impact Factor
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    ABSTRACT: The neuroprotective effect of hypothermia has been well established. The use of hypothermia for the treatment of stroke by systemic hypothermia is limited by the cooling rate and has severe complications. The goal of this study was to determine if local cerebral cooling via infusion could reduce infarction volume and improve the neurological outcome in a rat model of middle cerebral artery (MCA) occlusion. A hollow filament was used to block the MCA for 2 hours, and then the ischemic territory was locally infused with autocirculating cold arterial blood (13-15 degrees C). This cold blood infusion (<0.6 ml/min) significantly reduced the temperature of the MCA supplied territory to 32-34 degrees C in 5-10 minutes. This hypothermic procedure was maintained for 30 minutes. After evaluating the neurological score at 2 days and 28 days, all animals were euthanized and their brains were sectioned and stained with hematoxylin and eosin (H&E). Their infarct volumes were calculated. Local mild hypothermia in the brain was induced by autocirculation with a cold blood infusion, whereas the rectal temperature was maintained within the normal range. The infarction volume was significantly reduced and the neurological outcome was significantly improved (p<0.05) after MCA occlusion in Group 2 (hypothermia) compared with Group 1 (MCA occlusion) and Group 3 (normothermia). Local brain hypothermia induced by autocirculating cold arterial blood infused into ischemic tissue has neuroprotective effects.
    Neurological Research 05/2009; 31(4):340-5. DOI:10.1179/174313209X443982 · 1.45 Impact Factor
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    ABSTRACT: Interventional management of acute stroke can significantly increase recanalization rate of the occluded artery, however, this improvement is achieved at the expense of an increased incidence in symptomatic intracranial hemorrhage, which may markedly reduce the therapeutic benefit of this treatment. Hypothermia is one of the most promising neuroprotective approaches studied. It may also lower the risk of postischemic hemorrhage by reducing the activities of matrix metalloproteinases and blood-brain barrier disruption. But in most clinical studies, hypothermia is induced by surface cooling. It has two major drawbacks. (1) Several hours are required to reach the target body core temperature. (2) The incidence of adverse effects, such as impaired immune function, shivering, pneumonia, and cardiac arrhythmias/bradycardias, is high. Selective brain hypothermia without reducing body core temperature can theoretically address both problems of whole body cooling. So it is hypothesized that interventional management of acute stroke combined with catheter-based selective brain hypothermia may reduce the risk of postischemic hemorrhagic transformation, at the same time circumventing the bulk of negative side effects associated with systemic hypothermia.
    Medical Hypotheses 11/2008; 72(1):62-3. DOI:10.1016/j.mehy.2008.07.056 · 1.15 Impact Factor
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