Yuming Luo

Capital Medical University, Peping, Beijing, China

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Publications (5)13.76 Total impact

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    ABSTRACT: Objectives: We investigated the effect of a microcatheter-based selectively induced intra-arterial hypothermia on hemodynamic changes following transient cerebral ischemia in rats. Methods: Stroke was induced in male Sprague-Dawley rats by a two-hour middle cerebral artery occlusion (MCAO) using a microcatheter. After the two-hour MCAO, 0·9% cold saline (0°C) was selectively infused through a microcatheter. Cerebral blood flow (CBF) in the ischemic brain region was continuously monitored by Laser-Doppler flowmetry (LDF) during the procedure. Following ischemia/reperfusion, serial functional neurologic testing was performed, and cerebral infarct volume was evaluated after 48 hours. Results: The local cold saline infusion, via a microcatheter, achieved a rapid induction of brain hypothermia (cerebral cortex from 37·1 ± 0·3 to 30·7 ± 0·4°C; striatum from 37·5 ± 0·3 to 30·9 ± 0·5°C). When compared to the non-treatment group, the local cold saline infusion treatment reduced both post-ischemic hyperperfusion (about 40%, P < 0·01) and delayed post-ischemic hypoperfusion (P < 0·01), improved functional neurological testing (P < 0·01), and reduced both cerebral infarction volume (40·6 ± 5·3 vs. 61·7 ± 8·6%, P < 0·01) and cerebral edema (7·8 ± 2·6 vs.15·4 ± 3·2%, P < 0·01). Conclusion: Cold saline, when infused directly into the ischemic brain region, can confer robust neuroprotection by reducing immediate post-ischemic hyperperfusion and delayed post-ischemic hypoperfusion.
    Neurological Research 10/2014; · 1.18 Impact Factor
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    ABSTRACT: Stroke is deemed a worldwide leading cause of neurological disability and death, however, there is currently no promising pharmacotherapy for acute ischemic stroke aside from intravenous or intra-arterial thrombolysis. Yet because of the narrow therapeutic time window involved, thrombolytic application is very restricted in clinical settings. Accumulating data suggest that non-pharmaceutical therapies for stroke might provide new opportunities for stroke treatment. Here we review recent research progress in the mechanisms and clinical implications of non-pharmaceutical therapies, mainly including neuroprotective approaches such as hypothermia, ischemic/hypoxic conditioning, acupuncture, medical gases, transcranial laser therapy, etc. In addition, we briefly summarize mechanical endovascular recanalization devices and recovery devices for the treatment of the chronic phase of stroke and discuss the relative merits of these devices.
    Progress in Neurobiology 01/2014; · 9.04 Impact Factor
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    ABSTRACT: While recent studies suggest that remote ischemic postconditioning (RIP) therapy may be of benefit to patients with acute ischemic stroke, RIP's effects on intracerebral hemorrhage (ICH) still remains unclear. In the present study, the use of RIP in a rat model ICH was investigated to elucidate any potential beneficial or detrimental effects as determined by motor testing, blood brain barrier integrity, and brain water content, as well as aquaporin-4 (AQP-4) and matrix metalloproteinase-9 (MMP-9) expression. ICH was induced in Sprague-Dawley rats and they were randomized into either a control (n = 24) or RIP treatment (n = 24) group. RIP was performed by repetitive, brief occlusion and release of the bilateral femoral arteries. Functional outcome in each group was assessed by neurologic deficits on vibrissae-elicited forelimb placing test and a 12-point outcome scale. At 72 hours, brain blood volume, water content, blood-brain barrier (BBB) permeability, and protein expression of AQP-4 and MMP-9 were determined. This collagenase model yielded well-defined striatal hematomas. Vibrissae-elicited forelimb placement was significantly (P<0·01) affected by ICH. However, there was no significant difference between the RIP and control groups at either 24 or 72 hours. A 12-point neurological deficit score also failed to differentiate between the RIP and control. There were no significant differences between the two groups in cerebral blood volumes, brain water content, Evans blue extravasations, and expressions of AQP-4 and MMP-9. Although RIP did not show a beneficial effect in our ICH model, treatment with RIP did not exacerbate ICH.
    Neurological Research 01/2012; 34(2):143-8. · 1.18 Impact Factor
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    ABSTRACT: The neuroprotective effect of hypothermia has been well established. The use of hypothermia for the treatment of stroke by systemic hypothermia is limited by the cooling rate and has severe complications. The goal of this study was to determine if local cerebral cooling via infusion could reduce infarction volume and improve the neurological outcome in a rat model of middle cerebral artery (MCA) occlusion. A hollow filament was used to block the MCA for 2 hours, and then the ischemic territory was locally infused with autocirculating cold arterial blood (13-15 degrees C). This cold blood infusion (<0.6 ml/min) significantly reduced the temperature of the MCA supplied territory to 32-34 degrees C in 5-10 minutes. This hypothermic procedure was maintained for 30 minutes. After evaluating the neurological score at 2 days and 28 days, all animals were euthanized and their brains were sectioned and stained with hematoxylin and eosin (H&E). Their infarct volumes were calculated. Local mild hypothermia in the brain was induced by autocirculation with a cold blood infusion, whereas the rectal temperature was maintained within the normal range. The infarction volume was significantly reduced and the neurological outcome was significantly improved (p<0.05) after MCA occlusion in Group 2 (hypothermia) compared with Group 1 (MCA occlusion) and Group 3 (normothermia). Local brain hypothermia induced by autocirculating cold arterial blood infused into ischemic tissue has neuroprotective effects.
    Neurological Research 05/2009; 31(4):340-5. · 1.18 Impact Factor
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    ABSTRACT: Interventional management of acute stroke can significantly increase recanalization rate of the occluded artery, however, this improvement is achieved at the expense of an increased incidence in symptomatic intracranial hemorrhage, which may markedly reduce the therapeutic benefit of this treatment. Hypothermia is one of the most promising neuroprotective approaches studied. It may also lower the risk of postischemic hemorrhage by reducing the activities of matrix metalloproteinases and blood-brain barrier disruption. But in most clinical studies, hypothermia is induced by surface cooling. It has two major drawbacks. (1) Several hours are required to reach the target body core temperature. (2) The incidence of adverse effects, such as impaired immune function, shivering, pneumonia, and cardiac arrhythmias/bradycardias, is high. Selective brain hypothermia without reducing body core temperature can theoretically address both problems of whole body cooling. So it is hypothesized that interventional management of acute stroke combined with catheter-based selective brain hypothermia may reduce the risk of postischemic hemorrhagic transformation, at the same time circumventing the bulk of negative side effects associated with systemic hypothermia.
    Medical Hypotheses 11/2008; 72(1):62-3. · 1.18 Impact Factor