Liao Qinghua

Publications (2)3.31 Total impact

• Article: Totally extraperitoneal laparoscopic hernioplasty: the optimal surgical approach.

  Haitian Zhang, Jian Lin, Qinghua Liao, Naichang Xie, Xiaolong Gui, Jiehua Li, Shikai Hong, Xingan Qin, Yunfei Lu
  [show abstract] [hide abstract]
  ABSTRACT: The total extraperitoneal (TEP) laparoscopic repair of a groin hernia is increasingly being used because of less pain, rapid recovery, and low recurrence rate, but different variants of surgical approaches for TEP are reported by a number of centers and the merits and demerits associated with each approach are not well described. The aim of this study was to compare the clinical outcome of laparoscopic totally extraperitoneal inguinal hernioplasty (TEP) with 4 different variants of surgical approach. Between August 2004 and March 2008, 99 patients with unilateral inguinal hernia who underwent TEP without mesh fixation through 4 different variants of surgical approach depended on the anatomical positions of abdominal wall were enrolled in this prospective randomized study. The primary endpoints were operative time, incidence of peritoneal tear, and incidence of the arcuate line impeding the position of the mesh. Secondary endpoints were postoperative analgesic requirements and incidence of seroma. Ninety-nine patients underwent 4 surgical approaches, including the midline approach between the rectus muscle and the posterior rectus sheath (anterior to the posterior rectus sheath, MR) in 25 patients, the midline approach between the peritoneum and the posterior rectus sheath (posterior to the posterior rectus sheath, MP) in 25 patients, the lateral approach between the rectus muscle and the posterior rectus sheath (LR) in 25 patients, and the lateral approach between the peritoneum and the posterior rectus sheath (LP) in 24 patients. The groups were similar in age, weight, body height, and diagnostic categories of hernia. All cases were operated successfully without conversion to open surgery or transabdominal preperitoneal, and followed up for 6 to 43 months with no recurrence. The mean operating time was 55.5 minutes (25 to 130 min) and there was not significant difference between the groups in the operative time. The only type of complication was seroma formation that occurred in 20 patients retrieved without requiring drainage, and there was no significant difference in the incidence of seroma among 4 groups. Only in the MR group and the LR group, the arcuate line in 20 patients impedes the 15 x 10 cm polypropylene mesh positioning. Peritoneal tears were observed without routine closure in 36.7% of patients in the 2 groups of the lateral approach, whereas in only 12% in the 2 groups of the midline approach (P<0.01). Eleven patients required postoperative analgesics, and there was significant difference between the lateral approach groups and the midline approach groups (P<0.05). TEP is a mature technique and may be safely carried out with fewer complications and lower recurrence rate. Considering the body habitus of each patient and the large dissection surface area resulting in postoperative adhesions and bleeding, according to the principles of "Minimally Invasive Surgery" and the requirement of a maximal cosmetic result, the surgeon should select the midline approach between the peritoneum and the posterior rectus sheath as much as possible.
  Surgical laparoscopy, endoscopy & percutaneous techniques 12/2009; 19(6):501-5. · 1.23 Impact Factor
• Article: Mutation screening of the BRCA1 gene in sporadic breast cancer in southern Chinese populations.

  Zhang Haitian, Lu Yunfei, Zeng Jian, Lin Jian, Liao Qinghua, Wan Fuqiang
  [show abstract] [hide abstract]
  ABSTRACT: The comparison of freshly collected breast cancer tissue DNA with that of the matched blood specimens of 144 patients was studied, and breast tissue from 30 unrelated normal women without cancer was selected as controls. The entire BRCA1 coding sequence was amplified by PCR with primers especially designed for comprehensive mutation screening by single-strand conformation polymorphism (SSCP) analysis. Amplification and electrophoresis were repeated for the confirmation of altered migration patterns. Variant bands were subsequently cut from gels, resuspended in distilled water, subjected again to PCR, and then sequenced. The amplified fragments of exon 2 and exon 3, which were not suitable for SSCP (>300bp) were sequenced directly. A total of 20 nucleotide alterations were observed in the breast cancer tissue DNA, and all were single-base substitutions. Sixteen missense mutations (which change the coding from one amino acid to another) have been identified throughout the gene. Ten cases of single nucleotide changes in BRCA1 detected in the study without records in the BIC database consisted of two missense mutations in exon 5 (273C>G, 287A>T), one polymorphism in exon 11 (2630T>G), three missense mutations in exon 11 (2532T>G, 3191C>G, 3876C>A), one missense mutation in exon 12 (4285G>A), two missense mutations in exon 17 (5115T>C, 5116A>G), and one missense mutation in exon 18 (5206T>A). The same analysis was carried out on matched blood specimens for each of the 20 nucleotide alterations revealed to be present in the germline. No nucleotide alterations were detected in the controls. These results suggest that somatic mutations of BRCA1 are infrequent in sporadic breast cancer, and nucleotide alterations were more easily observed in the breast cancer tissue DNA. It is likely that there are other important susceptibility genes that remain to be identified in patients with sporadic breast cancer.
  Breast (Edinburgh, Scotland) 10/2008; 17(6):563-7. · 2.09 Impact Factor