Kailash C Bansal

National Bureau of Plant Genetic Resources, New Dilli, NCT, India

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Publications (33)129.61 Total impact

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    ABSTRACT: Crop wild relatives (CWRs) are invaluable gene sources for various traits of interest, yet these potential resources are themselves increasingly threatened by the impact of climate change as well as other anthropogenic and socio-economic factors. The prime goal of our research was to cover all aspects of wild Lens genetic resource management like species characterization, agro-morphological evaluation, diversity assessment, and development of representative sets for its enhanced utilization in lentil base broadening and yield improvement initiatives. We characterized and evaluated extensively, the global wild annual Lens taxa, originating from twenty seven counties under two agro-climatic conditions of India consecutively for three cropping seasons. Results on various qualitative and quantitative characters including two foliar diseases showed wide variations for almost all yield attributing traits including multiple disease resistance in the wild species, L. nigricans and L. ervoides accessions. The core set developed from the entire Lens taxa had maximum representation from Turkey and Syria, indicating rich diversity in accessions originating from these regions. Diversity analysis also indicated wide geographical variations across genepool as was reflected in the core set. Potential use of core set, as an initial starting material, for genetic base broadening of cultivated lentil was also suggested.
    PLoS ONE 09/2014; 9(9):e107781.. · 3.53 Impact Factor
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    ABSTRACT: Characterization of natural allelic diversity and understanding the genetic structure and linkage disequilibrium (LD) pattern in wild germplasm accessions by large-scale genotyping of informative microsatellite and single nucleotide polymorphism (SNP) markers is requisite to facilitate chickpea genetic improvement. Large-scale validation and high-throughput genotyping of genome-wide physically mapped 478 genic and genomic microsatellite markers and 380 transcription factor gene-derived SNP markers using gel-based assay, fluorescent dye-labelled automated fragment analyser and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass array have been performed. Outcome revealed their high genotyping success rate (97.5%) and existence of a high level of natural allelic diversity among 94 wild and cultivated Cicer accessions. High intra- and inter-specific polymorphic potential and wider molecular diversity (11–94%) along with a broader genetic base (13–78%) specifically in the functional genic regions of wild accessions was assayed by mapped markers. It suggested their utility in monitoring introgression and transferring target trait-specific genomic (gene) regions from wild to cultivated gene pool for the genetic enhancement. Distinct species/gene pool-wise differentiation, admixed domestication pattern, and differential genome-wide recombination and LD estimates/decay observed in a six structured population of wild and cultivated accessions using mapped markers further signifies their usefulness in chickpea genetics, genomics and breeding.
    PLoS ONE 09/2014; 9(9):e107484. · 3.53 Impact Factor
  • Journal of genetics. 08/2014; 93(2):561-6.
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    ABSTRACT: Resistance to stem rust and leaf rust in five D genome species of wheat viz., 267 accessions of Aegilops tauschii Coss., 39 of Ae. cylindrica Host, 17 of Ae. ventricosa Tausch, 4 of Ae. crassa Boiss. and 8 of Ae. juvenalis (Thell.) Eig were evaluated at adult plant stage. Two hundred and thirty nine (90 %) accessions of Ae. tauschii, 30 (77 %) of Ae. cylindrica, 16 (94 %) of Ae. ventricosa, 3 (75 %) of Ae. crassa Boiss. and 5 (62.5 %) of Ae. juvenalis were resistant to stem rust pathotypes prevalent in South India at Wellington under field condition. Invariably, all the accessions of the five species were resistant to leaf rust pathotypes. Quantitative measurement of disease using area under the disease progress curve revealed the slow progress of disease in the resistant accessions compared to susceptible check (Agra Local). Since all the five species have D genome, it could be concluded that the genes present in D genome might play a vital role in leaf rust resistance, but in case of stem rust resistance wide range of differential response was noticed. Among the species evaluated, Ae. tauschii was exploited to a larger extent, followed by Ae. ventricosa and Ae. cylindrica for leaf and stem rust resistance because of the homology of D genome with hexaploid bread wheat. While, Ae. crassa and Ae. juvenalis could not be utilized so far, possibly due to partial homology which makes the transfer of traits difficult. So, these species have considerable potential as a source of rust resistance and may enhance the existing gene pool of resistance to stem and leaf rusts.
    Genetic Resources and Crop Evolution 04/2014; 61(4). · 1.59 Impact Factor
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    ABSTRACT: 229 ReseaRch T he genus Cicer is a member of subfamily Papilionoideae and family Leguminosae, which comprises nine annual and 35 perennial species (van der Maesen, 1987). Of the 44 species, chick-pea (Cicer arietinum L.) is only the one cultivated on a large scale worldwide. Currently, it plays an important role in the agricul-tural production system and ranks third after dry bean (Phaseolus vulgaris L.) and field pea (Pisum sativum L.) in terms of world grain legume crop production. It also improves soil fertility through biological nitrogen fixation and provides nitrogen for crops in the farming system. Despite extensive breeding efforts to improve chickpea, lack of stable crop production continues to be a prime concern. This situation is aggravated by the regular shifting of chickpea production to marginal lands, where it faces a multitude of major stresses (Singh et al., 1998). Further, a low level of genetic variability within the cultivated gene pool has hampered chick-pea breeders in their efforts to develop widely adapted cultivars ABSTRACT Systematic characterization and evaluation of the wild gene pool for breeding purposes is a common practice in an increasing number of cultivated crop species. Substantial yield improvement of culti-vated chickpea (Cicer arietinum L.) has been lim-ited by the loss of useful genes for higher yield and lack of resistance to major biotic stresses. In the present study, a total of 88 wild accessions of six annual Cicer species viz., C. reticulatum Ladizinsky, C. echinospermum P.H. Davis, C. judaicum Boiss., C. pinnatifidum Jarb. & Spach, C. bijugum Rech. f., and C. yamashitae Kitam., along with three check varieties (controls) of cultivated chickpea namely, Pusa 256, Pusa 1103, and JG 11, were character-ized and evaluated for phenological and agro-morphological traits, including their reaction to the major fungal diseases [Ascochyta blight caused by Ascochyta rabiei (Pass.) Labr. and Botrytis gray mold caused by Botrytis cinerea Pers. ex. Fr.] and root knot nematode (Meliodogyne incognita). Wild Cicer accessions exhibited variation for some mor-phological traits, including plant pigmentation in C. reticulatum, C. judaicum, and C. pinnatifidum; number of leaflets leaf
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    ABSTRACT: 229 ReseaRch T he genus Cicer is a member of subfamily Papilionoideae and family Leguminosae, which comprises nine annual and 35 perennial species (van der Maesen, 1987). Of the 44 species, chick-pea (Cicer arietinum L.) is only the one cultivated on a large scale worldwide. Currently, it plays an important role in the agricul-tural production system and ranks third after dry bean (Phaseolus vulgaris L.) and field pea (Pisum sativum L.) in terms of world grain legume crop production. It also improves soil fertility through biological nitrogen fixation and provides nitrogen for crops in the farming system. Despite extensive breeding efforts to improve chickpea, lack of stable crop production continues to be a prime concern. This situation is aggravated by the regular shifting of chickpea production to marginal lands, where it faces a multitude of major stresses (Singh et al., 1998). Further, a low level of genetic variability within the cultivated gene pool has hampered chick-pea breeders in their efforts to develop widely adapted cultivars ABSTRACT Systematic characterization and evaluation of the wild gene pool for breeding purposes is a common practice in an increasing number of cultivated crop species. Substantial yield improvement of culti-vated chickpea (Cicer arietinum L.) has been lim-ited by the loss of useful genes for higher yield and lack of resistance to major biotic stresses. In the present study, a total of 88 wild accessions of six annual Cicer species viz., C. reticulatum Ladizinsky, C. echinospermum P.H. Davis, C. judaicum Boiss., C. pinnatifidum Jarb. & Spach, C. bijugum Rech. f., and C. yamashitae Kitam., along with three check varieties (controls) of cultivated chickpea namely, Pusa 256, Pusa 1103, and JG 11, were character-ized and evaluated for phenological and agro-morphological traits, including their reaction to the major fungal diseases [Ascochyta blight caused by Ascochyta rabiei (Pass.) Labr. and Botrytis gray mold caused by Botrytis cinerea Pers. ex. Fr.] and root knot nematode (Meliodogyne incognita). Wild Cicer accessions exhibited variation for some mor-phological traits, including plant pigmentation in C. reticulatum, C. judaicum, and C. pinnatifidum; number of leaflets leaf
    Crop Science 01/2014; 54(54):229-239. · 1.51 Impact Factor
  • Kailash C Bansal, Ajay K Singh
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    ABSTRACT: Eggplant (Solanum melongena L.) is an important vegetable crop of tropical and temperate regions of the world. Here we describe a procedure for eggplant plastid transformation, which involves preparation of explants, biolistic delivery of plastid transformation vector into green stem segments, selection procedure, and identification of the transplastomic plants. Shoot buds appear from cut ends of the stem explants following 5-6 weeks of spectinomycin selection after bombardment with the plastid transformation vector containing aadA gene as selectable marker. Transplastomic lines are obtained after the regenerated shoots are subjected to several rounds of spectinomycin selection over a period of 9 weeks. Homoplasmic transplastomic lines are further confirmed by spectinomycin and streptomycin double selection. The transplastomic technology development in this plant species will open up exciting possibilities for improving crop performance, metabolic engineering, and the use of plants as factories for producing biopharmaceuticals.
    Methods in molecular biology (Clifton, N.J.) 01/2014; 1132:305-16. · 1.29 Impact Factor
  • Lakshmi Kasirajan, Boomiraj Kovilpillai, Kailash Chander Bansal
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    ABSTRACT: We have developed marker-free transgenic wheat using a transcription factor, AtDREB1A cloned from Arabidopsis. Southern hybridization confirmed a transgenic event with a single copy insertion. PCR analysis of the T1 plants showed four were positive only for AtDREB1A. A T1 plant (HRCB3#17-37) was marker-free and had good expression of drought tolerance in comparison with untransformed plants. The leaf relative water content of this T1 transgenic plant was 12-15 % higher than that of the wild type during stress with an 8 % higher yield under water deficit conditions compared with wild type plants.
    Biotechnology Letters 12/2013; · 1.85 Impact Factor
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    Kailash C Bansal, Sangram Keshari Lenka, Tapan Mondal
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    ABSTRACT: Abstract To meet the challenges of climate change, exploring natural diversity in the existing plant genetic resource pool as well as creation of new mutants through chemical mutagenesis and molecular biology is needed for developing climate-resilient elite genotypes. Ever-increasing area under existing abiotic stresses as well as emerging abiotic stress factors and their combinations have further added to the problems of the current crop improvement programmes. However, with the advancement in modern techniques such as next-generation sequencing technologies, it is now possible to generate on a whole-genome scale, genomic resources for crop species at a much faster pace with considerably less efforts and money. The genomic resources thus generated will be useful for various plant breeding applications such as marker-assisted breeding for gene introgression, mapping QTLs or identifying new or rare alleles associated with a particular trait. In this article, we discuss various aspects of generation of genomic resources and their utilization for developing abiotic stress-tolerant crops to ensure sustainable agricultural production and food security in the backdrop of rapid climate change.
    Plant Breeding 12/2013; · 1.18 Impact Factor
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    ABSTRACT: Wild Lens taxa are invaluable sources of useful traits for broadening genetic base of cultivated lentil. Nine inter-sub-specific and interspecific crosses were made successfully between cultivated (Lens culinaris ssp. culinaris) and wild lentils (L. culinaris ssp. orientalis, odemensis, lamottei and ervoides). The effect of species groups, day length and temperature on crossability in lentils was evident under normal winter sowing in New Delhi and in summer Himalayan nursery at Sangla in Himachal Pradesh, India, although pollen fertility assessed in all the cross-combinations showed no significant variation. True hybridity of nine inter-sub-specific and interspecific crosses was confirmed through morphological and molecular (ISSR) markers, in which three of 120 primers could confirm the hybridity of all the crosses. All cross-combinations were also studied for important quantitative traits related to yield. The range, mean and coefficient of variation were estimated in parental lines, F1 and F2 generations to determine the extent of variability generated in cultivated lentils through the introgression of genes from wild L. taxa. A high level of heterosis was observed in F1 crosses for important traits studied. Substantially higher variations for seed yield and its attributing traits were exhibited in F2 generations indicating transgressive segregation. The results of the present investigation revealed that wild L. taxa can be successfully exploited for lentil improvement programmes, and the variations generated could be easily utilized for broadening the genetic base of cultivated lentil gene pool for improving the yield as well as wider adaptation.
    Plant Breeding 12/2013; 132(6). · 1.18 Impact Factor
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    ABSTRACT: Fruit ripening process is associated with change in carotenoid profile and accumulation of lycopene in tomato (Solanum lycopersicum L.). In this study, we quantified the β-carotene and lycopene content at green, breaker and red-ripe stages of fruit ripening in eight tomato genotypes by using high-performance liquid chromatography. Among the genotypes, lycopene content was found highest in Pusa Rohini and lowest in VRT-32-1. To gain further insight into the regulation of lycopene biosynthesis and accumulation during fruit ripening, expression analysis of nine carotenoid pathway-related genes was carried out in the fruits of high lycopene genotype—Pusa Rohini. We found that expression of phytoene synthase and β-carotene hydroxylase-1 was four and thirty-fold higher, respectively, at breaker stage as compared to red-ripe stage of fruit ripening. Changes in the expression level of these genes were associated with a 40% increase in lycopene content at red-ripe stage as compared with breaker stage. Thus, the results from our study suggest the role of specific carotenoid pathway-related genes in accumulation of high lycopene during the fruit ripening processes.
    Journal of Genetics 10/2013; · 0.88 Impact Factor
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    ABSTRACT: Carotenoid metabolism is regulated by several genes encoding carotenoid biosynthetic pathway enzymes. In the present study, a fruit transcriptome in tomato (Solanum lycopersicum) was compared between high lycopene accumulating genotype EC-521086 and low lycopene accumulating genotype VRT-32-1 at three different stages (green, breaker and red) of fruit ripening. This analysis led to the identification of 2,558 differentially expressed genes at three stages of fruit ripening. Among these genes, 123 were carotenoid-correlated genes. Quantitative RT-PCR analysis revealed high expression of genes encoding enzymes involved in lycopene biosynthesis like IPP isomerase, phytoene synthase, phytoene desaturase, z-carotene desaturase; and comparatively lower expression of genes encoding enzymes involved in lycopene catabolism like lycopene cyclase, carotenoid e-ring hydroxylase, zeaxanthin epoxidase, violaxanthin de-epoxidase and neoxanthin synthase in EC-521086, thereby possibly explaining the high lycopene content in EC-521086 as compared with the low lycopene genotype VRT-32-1. Further, the EC-521086 genotype exhibited high expression of the TOMATO AGAMOUSLIKE 1 (TAGL1) MADS box gene—a positive regulator of lycopene accumulation—at breaker stage, and low expression of the ethylene receptor LeETR4 gene—a negative regulator of trans-lycopene and β-carotene accumulation, at the red stage of fruit ripening. Our results clearly demonstrate the role of specific genes in accumulation of high lycopene in the EC-521086 tomato genotype during the fruit ripening processes.
    Plant Molecular Biology Reporter 06/2013; 31:1384 – 1396. · 5.32 Impact Factor
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    Nature Biotechnology 01/2013; · 32.44 Impact Factor
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    ABSTRACT: Chickpea (Cicer arietinum) is the second most widely grown legume crop after soybean, accounting for a substantial proportion of human dietary nitrogen intake and playing a crucial role in food security in developing countries. We report the ∼738-Mb draft whole genome shotgun sequence of CDC Frontier, a kabuli chickpea variety, which contains an estimated 28,269 genes. Resequencing and analysis of 90 cultivated and wild genotypes from ten countries identifies targets of both breeding-associated genetic sweeps and breeding-associated balancing selection. Candidate genes for disease resistance and agronomic traits are highlighted, including traits that distinguish the two main market classes of cultivated chickpea-desi and kabuli. These data comprise a resource for chickpea improvement through molecular breeding and provide insights into both genome diversity and domestication.
    Nature Biotechnology 01/2013; · 32.44 Impact Factor
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    ABSTRACT: Identification of genes that are coexpressed across various tissues and environmental stresses is biologically interesting, since they may play coordinated role in similar biological processes. Genes with correlated expression patterns can be best identified by using coexpression network analysis of transcriptome data. In the present study, we analyzed the temporal-spatial coordination of gene expression in root, leaf and panicle of rice under drought stress and constructed network using WGCNA and Cytoscape. Total of 2199 differentially expressed genes (DEGs) were identified in at least three or more tissues, wherein 88 genes have coordinated expression profile among all the six tissues under drought stress. These 88 highly coordinated genes were further subjected to module identification in the coexpression network. Based on chief topological properties we identified 18 hub genes such as ABC transporter, ATP-binding protein, dehydrin, protein phosphatase 2C, LTPL153 - Protease inhibitor, phosphatidylethanolaminebinding protein, lactose permease-related, NADP-dependent malic enzyme, etc. Motif enrichment analysis showed the presence of ABRE cis-elements in the promoters of > 62% of the coordinately expressed genes. Our results suggest that drought stress mediated upregulated gene expression was coordinated through an ABA-dependent signaling pathway across tissues, at least for the subset of genes identified in this study, while down regulation appears to be regulated by tissue specific pathways in rice.
    Bioinformation 01/2013; 9(2):72-8. · 0.50 Impact Factor
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    ABSTRACT: Unrevealing the molecular details of plant response and defense against abiotic stress factors such as drought, salt and temperature extremes is a crucial and challenging issue in plant research. Functional genomics and computational biology enhance pace of molecular dissection of abiotic stress response mechanisms. During the past two decades several QTLs associated with abiotic stress responses of plants have been mapped. A QTL is a chromosomal region that contains a gene or genes that influence a quantitative trait. QTL mapping approach is applied frequently to map chromosomal regions that contribute significantly to a complex trait. The availability of complete genome sequence of important model plants namely Arabidopsis and rice, QTL databases and mapping tools facilitate genomics-based strategies for gene discovery, coupled with high-throughput techniques speed-up gene discovery for abiotic stress tolerance.
    03/2012: pages 321-335; , ISBN: 978-3-527-63293-0
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    ABSTRACT: In post-genomic era, bioinformatic tools allow us to explore and reconstruct the precise gene interaction network. To deduce the function of uncharacterized gene, genetic network by co-regulatory analysis from an expression data is a foremost approach. In this study, we report comprehensive identification of co-expressed MYB gene modules in rice. MYB transcription factor family is involved in phenylpropanoid and flavonoid biosynthesis and various other metabolic and developmental processes. By a reiterative database exploration, 249 potential OsMYB genes were retrieved. Computational analysis has shown the presence of several other functional domains including WD domain, G-beta repeat, response regulator receiver domain, BTB/POZ domain, SWIRM/Zinc finger domain and many more. Several studies have pointed out their involvement in a range of biological processes, revealing that a large number of MYB genes are transcriptionally regulated under conditions of biotic and/or abiotic stress. To investigate the existence of MYB co-regulatory network, a whole genome MYB expression study was carried out in rice. We identified the existence of co-expression clusters comprising phylogenetically related MYB genes, suggesting that specific sets of MYB genes might act in co-regulatory network. Thus, the co-expression networks identified in this study illustrate gene cooperation pathways that have not been identified by classical genetic.
    Int'l Conf. Bioinformatics and Computational Biology | BIOCOMP'11 |; 07/2011
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    ABSTRACT: Developing crops that are better adapted to abiotic stresses is important for food production in many parts of the world today. Anticipated changes in climate and its variability, particularly extreme temperatures and changes in rainfall, are expected to make crop improvement even more crucial for food production. Here, we review two key biotechnology approaches, molecular breeding and genetic engineering, and their integration with conventional breeding to develop crops that are more tolerant of abiotic stresses. In addition to a multidisciplinary approach, we also examine some constraints that need to be overcome to realize the full potential of agricultural biotechnology for sustainable crop production to meet the demands of a projected world population of nine billion in 2050.
    Trends in Plant Science 04/2011; 16(7):363-71. · 11.81 Impact Factor
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    ABSTRACT: Genetic improvement in drought tolerance in rice is the key to save water for sustainable agriculture. Drought tolerance is a complex trait and involves interplay of a vast array of genes. Several genotypes of rice have evolved features that impart tolerance to drought and other abiotic stresses. Comparative analysis of drought stress-responsive transcriptome between drought-tolerant (DT) landraces/genotypes and drought-sensitive modern rice cultivars will unravel novel genetic regulatory mechanisms involved in stress tolerance. Here, we report transcriptome analysis in a highly DT rice landrace, Nagina 22 (N22), versus a high-yielding but drought-susceptible rice variety IR64. Both genotypes exhibited a diverse global transcriptional response under normal and drought conditions. Gene ontology (GO) analysis suggested that drought tolerance of N22 was attributable to the enhanced expression of several enzyme-encoding genes. Drought susceptibility of IR64 was attributable to significant down-regulation of regulatory components that confer drought tolerance. Pathway analysis unravelled significant up-regulation of several components of carbon fixation, glycolysis/gluconeogenesis and flavonoid biosynthesis and down-regulation of starch and sucrose metabolism in both the cultivars under drought. However, significant up-regulation of α-linolenic acid metabolic pathway observed in N22 under drought appears to be in good agreement with high drought tolerance of this genotype. Consensus cis-motif profiling of drought-induced co-expressed genes led to the identification of novel cis-motifs. Taken together, the results of the comparative transcriptome analysis led to the identification of specific genotype-dependent genes responsible for drought tolerance in the rice landrace N22.
    Plant Biotechnology Journal 04/2011; 9(3):315-27. · 6.28 Impact Factor
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    ABSTRACT: Genetically engineered tomato (Lycopersicon esculentum) with the ability to synthesize glycinebetaine was generated by introducing the codA gene encoding choline oxidase from Arthrobacter globiformis. Integration of the codA gene in transgenic tomato plants was verified by PCR analysis and DNA blot hybridization. Transgenic expression of gene was verified by RT-PCR analysis and RNA blot hybridization. The codA-transgenic plants showed higher tolerance to salt stress during seed germination, and subsequent growth of young seedlings than wild-type plants. The codA transgene enhanced the salt tolerance of whole plants and leaves. Mature leaves of codA-transgenic plants revealed higher levels of relative water content, chlorophyll content, and proline content than those of wild-type plants under salt and water stresses. Results from the current study suggest that the expression of the codA gene in transgenic tomato plants induces the synthesis of glycinebetaine and improves the tolerance of plants to salt and water stresses.
    Journal of plant physiology 02/2011; 168(11):1286-94. · 2.50 Impact Factor

Publication Stats

239 Citations
129.61 Total Impact Points

Institutions

  • 2013–2014
    • National Bureau of Plant Genetic Resources
      New Dilli, NCT, India
  • 2006–2011
    • National Research Centre on Plant Biotechnology
      Old Delhi, NCT, India
  • 2010
    • Indian Agricultural Research Institute
      • Water Technology Centre
      New Delhi, NCT, India
  • 2008
    • Central Institute of Fisheries Technology
      Verāval, Gujarāt, India