Stephan Winter

Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswyck, Lower Saxony, Germany

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Publications (32)53.52 Total impact

  • Dennis Knierim · Edgar Maiss · Wulf Menzel · Stephan Winter · Lawrence Kenyon ·
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    ABSTRACT: Leaf samples from Thailand of Sauropus androgynus (L.) Merr. plants presenting leaf yellowing symptoms were screened by reverse transcription polymerase chain reaction for the presence of polerovirus infection. In five of eight samples, a polerovirus was detected, and in four of these five polerovirus-positive samples, a begomovirus infection had been detected in a previous study. The complete polerovirus genome sequence was determined from the sample which was not also infected with a begomovirus. Analysis of the sequence revealed the typical genome organization of a polerovirus. The highest nucleotide sequence identity to any other polerovirus species sequence in GenBank was 49% to pepper yellow leaf curl virus (PYLCV), and the highest amino acid sequence identity of all deduced protein sequences was 70% with the coat protein of PYLCV. Based on these findings, we propose this novel polerovirus species be named Sauropus yellowing virus (SaYV).
    Journal of Phytopathology 09/2015; 163(9):695-702. DOI:10.1111/jph.12365 · 0.82 Impact Factor
  • Dennis Knierim · Edgar Maiss · Lawrence Kenyon · Stephan Winter · Wulf Menzel ·
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    ABSTRACT: Luffa aphid-borne yellows virus (LABYV) was proposed as the name for a previously undescribed polerovirus based on partial genome sequences obtained from samples of cucurbit plants collected in Thailand between 2008 and 2013. In this study, we determined the first full-length genome sequence of LABYV. Based on phylogenetic analysis and genome properties, it is clear that this virus represents a distinct species in the genus Polerovirus. Analysis of sequences from sample TH24, which was collected in 2010 from a luffa plant in Thailand, reveals the presence of two different full-length genome consensus sequences.
    Archives of Virology 07/2015; 160(10). DOI:10.1007/s00705-015-2529-5 · 2.39 Impact Factor
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    06/2015; 31. DOI:10.5197/j.2044-0588.2015.031.027
  • Dennis Knierim · Edgar Maiß · Wulf Menzel · Stephan Winter · Lawrence Kenyon ·

    47. Jahrestreffens des DPG-Arbeitskreises "Viruskrankheiten der Pflanzen", Berlin, Germany; 03/2015
  • W. Menzel · J. Hamacher · S. Winter ·
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    ABSTRACT: During investigation of Gaillardia aristata breeding material several plants reacted strongly (DAS-ELISA) with a commercially available Chrysanthemum virus B (CVB) antiserum. In order to confirm the identity of the virus, a part of its replicase gene was sequenced, showing just 26% amino acid sequence identity to CVB. This prompted us to determine the entire genome of this virus isolate. The complete genome was 8659 nt in length (excluding poly-A tail) and contained six open reading frames. The genome organisation resembled that of typical carlaviruses. The replicase (70%) and CP (71%) showed the highest aa sequence identities to Phlox virus S (PhVS), being well below the species demarcation threshold of 80%. The remaining ORFs (TGB1-3, NABP) also showed the highest aa sequence identities to PhVS, ranging from 59 (TGB3) to 77% (NABP). In addition, the CVB antiserum was tested with other Carlavirus isolates available at the DSMZ plant virus collection. Besides CVB and the new Carlavirus from Gaillardia, it showed a strong cross-reaction (DAS-ELISA) with isolates of Kalanchoe latent virus, Potato virus S, Passiflora latent virus and Helenium virus S.
    Acta horticulturae 02/2015; 1072(1072):129-134. DOI:10.17660/ActaHortic.2015.1072.15
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    Wulf Menzel · Kaveh Bananej · Stephan Winter ·
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    ABSTRACT: Cucumber Bulgarian latent virus (CBLV) was first reported from cucumber in Bulgaria in 2003 and has been assigned to the genus Tombusvirus. Ten years after the first and only report of CBLV, an isolate from a cucumber sample collected in Iran was characterized. Its complete genomic sequence was determined and analysed. Except for the coat protein, CBLV shows the highest sequence identities to the isolates of other species of the genus Tombusvirus. However, sequence comparison and phylogenetic analyses based on the coat protein (CP) revealed that CBLV is more closely related to the genus Aureusvirus rather than to the isolates of the genus Tombusvirus. The sequence identities to some aureusviruses are above the species demarcation threshold value, demonstrating that CBLV is an unusual tombusvirus species. This suggests that it is necessary to review the CP threshold value for species demarcation in the genus Aureusvirus. In addition, CBLV has an intermediate genome size compared to other tombus- and aureusviruses. Several polyclonal antisera raised against different tombus- and aureusviruses were used to assess the serological relation to CBLV. The ELISA results indicate that CBLV is not serologically related to any of those tested.
    Journal of Phytopathology 11/2014; 163(11). DOI:10.1111/jph.12340 · 0.82 Impact Factor
  • Adane D Abraham · Wulf Menzel · Berhanu Bekele · Stephan Winter ·
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    ABSTRACT: Etiological studies of a recently emerged bushy top disease of tobacco in Ethiopia indicated that a ~4.5-kb dsRNA from infected plants represents an umbravirus, whereas a smaller band (~0.5 kb) is that of a new satellite RNA. Potato leafroll virus was also consistently associated with the disease. The three agents, whose experimental host ranges are restricted to members of the family Solanaceae, always occurred together in field samples and are transmitted together by the aphid Myzus persicae nicotianae. The umbravirus, which represents a new species, is most closely related to groundnut rosette virus, and the name Ethiopian tobacco bushy top virus is proposed.
    Archives of Virology 08/2014; 159(12). DOI:10.1007/s00705-014-2202-4 · 2.39 Impact Factor
  • Dennis Knierim · Edgar Maiß · Wulf Menzel · Stephan Winter · Lawrence Kenyon ·

    46. Jahrestreffens des DPG-Arbeitskreises "Viruskrankheiten der Pflanzen", Siebeldingen, Germany; 03/2014
  • Kaveh Bananej · Nasibeh Kianfar · Aisan Vahdat · Stephan Winter · Wulf Menzel ·
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    ABSTRACT: During surveys conducted in 2012–2013, viruslike symptoms of chlorotic spots with, in some cases, a necrotic centre in older leaves were observed in field- and greenhouse-grown cucumber (Cucumis sativus L.), melon (C. melo L.) and squash (Cucurbita sp.) in the major cucurbit cultivation regions in Iran. Leaf samples were collected and tested for the presence of Cucumber leaf spot virus (CLSV, genus Aureusvirus, family Tombusviridae) by a virus specific double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). CLSV was detected in four of eight surveyed provinces in melon, cucumber and squash. When plant sap of ELISA positive samples was used to mechanically inoculate healthy squash plants, chlorotic spots with, in some cases, necrotic centres were observed on the inoculated leaves 20–25 days postinoculation. The presence of CLSV was confirmed by reverse transcription polymerase chain reactions using specific primers amplifying the entire coat protein gene of CLSV. Sequence comparison with sequences available at GenBank showed 93% nucleotide sequence identity to CLSV isolates from Israel (DQ227315) and Canada (EU127904), the only CLSV coat protein sequences available. To our knowledge, this is the first report of the occurrence of CLSV in Iran.
    Journal of Phytopathology 03/2014; 162(3):205-208. DOI:10.1111/jph.12178 · 0.82 Impact Factor
  • Kaveh Bananej · Nasibeh Kianfar · Stephan Winter · Wulf Menzel ·
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    ABSTRACT: Yellowing symptoms of greenhouse- and open field-grown cucurbit crops are becoming increasingly important in many cucurbit growing regions of the world, and particularly in Iran. A survey was conducted from 2011 to 2012 in eight major cucurbit growing regions in Iran. Yellowing and specifically vein clearing symptoms were observed in many cucumber plants grown in greenhouses and open fields, suggesting the presence of Cucumber vein yellowing virus (CVYV, genus Ipomovirus, family Potyviridae). The identification of CVYV was carried out with a specific triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and reverse transcription (RT)-PCR. CVYV was detected in 42% of the collected samples, in all surveyed provinces, except Bushehr. CVYV was also detected in melon and cucumber crops grown in open fields. These results indicate that CVYV is widely distributed on these two cucurbit species in the major cucumber growing areas of Iran. CVYV positive samples were also tested, using DAS-ELISA, for the presence of Cucurbit chlorotic yellows virus (CCYV) and Cucurbit yellow stunting disorder virus (CYSDV), two criniviruses reported previously to occur in Iran. Double or triple infections of CCYV and CYSDV occurred in 49 of 166 of the CVYV-infected plants. The CVYV and CCYV combined infections were more prevalent than CVYV and CYSDV combined infections. TAS-ELISA positive samples were used to mechanically inoculate healthy cucumber plants, and mild vein yellowing was observed on the inoculated leaves. Identical symptoms were also observed on whitefly inoculated healthy cucumber plants. The presence of CVYV in mechanically and whitefly inoculated plants was confirmed by TAS-ELISA and RT-PCR. Sequence analysis revealed that the Iranian isolate of CVYV was more closely related to Spanish isolates than to isolates from Jordan. Phylogenetic analysis showed that CVYV isolates can be divided into two phylogenetic groups (I and II). Despite the close vicinity of Jordan to Iran, the Iranian CVYV isolate clustered with Spanish isolates in group I and not with the Jordanian isolates of group II.
    Phytopathologia Mediterranea 01/2014; 53(2):269-276. DOI:10.14601/Phytopathol_Mediterr-13189 · 0.97 Impact Factor
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    ABSTRACT: Despite the seemingly continuous development of newer and ever more elaborate methods for detecting and identifying viruses, very few of these new methods get adopted for routine use in testing laboratories, often despite the many and varied claimed advantages they possess. To understand why the rate of uptake of new technologies is so low, requires a strong understanding of what makes a good routine diagnostic tool to begin. This can be done by looking at the two most successfully established plant virus detection methods: enzyme-linked immunosorbant assay (ELISA) and more recently introduced real-time polymerase chain reaction (PCR). By examining the characteristics of this pair of technologies, it becomes clear that they share many benefits, such as an industry standard format and high levels of repeatability and reproducibility. These combine to make methods that are accessible to testing labs, which are easy to establish and robust in their use, even with new and inexperienced users. Hence, to ensure the establishment of new techniques it is necessary to not only provide benefits not found with ELISA or real-time PCR, but also to provide a platform that is easy to establish and use. In plant virus diagnostics, recent developments can be clustered into three core areas: 1. techniques that can be performed in the field or resource poor locations (e.g., loop-mediated isothermal amplification LAMP); 2. multiplex methods that are able to detect many viruses in a single test (e.g., Luminex bead arrays); and 3. methods suited to virus discovery (e.g., next generation sequencing, NGS). Field based methods are not new, with Lateral Flow Devices (LFDs) for the detection being available for a number of years now. However the widespread uptake of this technology remains poor. LAMP does offer significant advantages over LFDs, in terms of sensitivity and generic application, but still faces challenges in terms of establishment. It is likely that the main barrier to the uptake of field-based technologies is behavioral influences, rather than specific concerns about the performance of the technologies themselves. To overcome this, a new relationship will need to develop between centralized testing laboratories offering services and those requiring tests; a relationship which is currently in its infancy. Looking further into the future, virus discovery and multiplex methods seem to converge as NGS becomes ever cheaper, easier to perform and can provide high levels of multiplexing without the use of virus specific reagents. So ultimately the key challenge from a routine testing lab perspective will not be one of investment in platforms-which could even be outsourced to commercial sequencing services-but one of having the skills and expertise to analyse the large datasets generated and their subsequent interpretation. In conclusion, only time will tell which of the next-generation of methods currently in development will become the routine diagnostics of the future. This will be determined through a combination of factors. And while the technology itself will have to offer performance advantages over existing methods in order to supplant them, it is likely to be human factors e.g., the behaviors of end users, laboratories and policy makers, the availability of appropriate expertise, that ultimately determine which ones become established. Hence factors cannot be ignored and early engagement with diagnostic stakeholders is essential.
    Virus Research 12/2013; DOI:10.1016/j.virusres.2013.12.007 · 2.32 Impact Factor
  • Wulf Menzel · George Thottappilly · Stephan Winter ·

    Archives of Virology 10/2013; 159(3). DOI:10.1007/s00705-013-1845-x · 2.39 Impact Factor
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    ABSTRACT: The availability of characterised reference isolates of plant pathogens is of crucial importance for research and diagnostic laboratories. The Q-bank Plant Viruses and Viroids database contains data and information on plant viruses and viroids, with the unique feature that it is linked to specimens present in publicly available physical collections. The Q-bank database aims to share data and information on the virus and viroid species and their availability between research and diagnostic laboratories. Currently the database focuses on regulated virus species. In future more plant viruses and viroids will be included to provide a comprehensive data and information system. The curators invite virologists worldwide to participate in this international initiative by making their data and isolates available via Q-bank ( La base de données et les collections Virus et Viroïdes des Plantes de Q-bank La disponibilité d'isolats de référence caractérisés est d'une importance cruciale pour les laboratoires de recherche et de diagnostic. La base de données de Q-bank sur les Virus et Viroïdes des plantes contient des données et des informations sur les virus et viroïdes, avec l'avantage d’être liée à des spécimens présents dans des collections physiques qui sont disponibles publiquement. La base de données Q-bank vise à permettre le partage de données et d'informations sur les espèces de virus et de viroïdes et leur mise à disposition entre laboratoires de recherche et de diagnostic. Actuellement, la base de données se concentre sur les espèces de virus réglementées. A l'avenir, d'autres virus et viroïdes des végétaux seront ajoutés pour fournir un système de données et d'informations cohérent et complet. Les curateurs invitent les virologistes dans le monde entier à participer à cette initiative internationale en rendant leurs données et leurs isolats disponibles au travers de Q-bank ( Бaзa дaнныч о вирусaч и вироидaч рaстений и коллекции Q-bank Для иccлeдoвaтeльcкиx и диaгнocтичecкиx лaбopaтopий дocтyпнocть oxapaктepизoвaнныx cпpaвoчныx изoлятoв пaтoгeнoв pacтeний имeeт пepвocтeпeннoe знaчeниe. Бaзa дaнныx пoд нaзвaниeм «The Q-bank Plant Viruses & Viroids» coдepжит дaнныe и инфopмaцию o виpycax и виpoидax pacтeний и имeeт тy yникaльнyю ocoбeннocть, чтo oнa cвязaнa c экзeмпляpaми, нaличecтвyющими в пyбличнo дocтyпныx кoллeкцияx. Бaзa дaнныx Q-bank cтaвит cвoeй цeлью oбмeн дaнными и инфopмaциeй o видax виpycoв и виpoидax и иx дocтyпнocти для иccлeдoвaтeльcкиx и диaгнocтичecкиx лaбopaтopий. B нacтoящee вpeмя бaзa дaнныx cocpeдoтoчeнa нa peгyлиpyeмыx видax виpycoв. B бyдyщeм в нee бyдeт включeнo бoльшee кoличecтвo виpycoв и виpoидoв pacтeний, c тeм чтoбы oбecпeчивaлacь пoлнoтa дaнныx и инфopмaциoнныx cиcтeм. Кypaтopы этoй бaзы пpeдлaгaют виpoлoгaм вceгo миpa пoyчacтвoвaть в этoй мeждyнapoднoй инициaтивe пyтeм пpeдocтaвлeния cвoиx дaнныx и изoлятoв чepeз Q-bank (<>).
    Bulletin OEPP/EPPO Bulletin 08/2013; 43(2). DOI:10.1111/epp.12033
  • Kaveh Bananej · W. Menzel · N. Kianfar · A. Vahdat · S. Winter ·

    Plant Disease 07/2013; 97(7):1005. DOI:10.1094/PDIS-01-13-0125-PDN · 3.02 Impact Factor
  • K. Hamed · W. Menzel · M. E. Mohamed · K. A. Bakheet · S. Winter ·

    Plant Disease 04/2013; 97(4):562-562. DOI:10.1094/PDIS-11-12-1018-PDN · 3.02 Impact Factor

  • Plant Disease 07/2012; 96(7):1075-1075. DOI:10.1094/PDIS-03-12-0253-PDN · 3.02 Impact Factor
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    Wulf Menzel · Joachim Hamacher · Sandra Weissbrodt · Stephan Winter ·
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    ABSTRACT: The complete sequence of the RNA 3 of a virus causing chlorosis in Impatiens in Germany was determined and identified as an isolate of Bacopa chlorosis virus (BaCV, genus Ilarvirus). BaCV has previously only been reported from bacopa in the USA, but no coat protein (CP) sequence has been previously available. Both RNA 3 encoded proteins, CP and movement protein, showed highest sequence identity to Parietaria mottle virus, a subgroup 1 ilarvirus. Attempts to purify BaCV failed, so an antiserum was raised against a recombinant CP. The polyclonal antiserum so produced allowed specific detection of BaCV but showed no serological cross-reaction with other ilarviruses and was unsuitable for immunoelectron microscopy. The host range includes many important flowering plant species, highlighting the potential threat BaCV might pose for the horticultural industry. This is the first report of BaCV occurring in Germany and outside the US.
    Journal of Phytopathology 03/2012; 160(3):163-165. DOI:10.1111/j.1439-0434.2011.01877.x · 0.82 Impact Factor

  • Archives of Virology 01/2012; 157(4). DOI:10.1007/s00705-012-1228-8 · 2.39 Impact Factor
  • Adane Abraham · Wulf Menzel · H Josef Vetten · Stephan Winter ·
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    ABSTRACT: The complete genome of a tomato mild mottle virus (ToMMV) isolate was analysed, and some biological features were characterized. The ssRNA genome of ToMMV from Ethiopia encompasses 9283 nucleotides (excluding the 3' poly(A) tail) and encodes a polyprotein of 3011 amino acids. Phylogenetic and pairwise comparisons with other members of the family Potyviridae revealed that ToMMV is the most divergent member of the genus Ipomovirus, with a genome organization similar to that of members of the species Sweet potato mild mottle virus, the type species of the genus. In contrast to earlier reports, ToMMV isolates from Yemen and Ethiopia were not transmitted by the aphid Myzus persicae, but they were transmitted very erratically by the whitefly Bemisia tabaci. A comparison of the 3'-proximal sequences of different isolates provided evidence for geographically associated genetic variation.
    Archives of Virology 11/2011; 157(2):353-7. DOI:10.1007/s00705-011-1167-9 · 2.39 Impact Factor
  • Wulf Menzel · Mathew M Abang · Stephan Winter ·

    Archives of Virology 09/2011; 156(12):2309-11. DOI:10.1007/s00705-011-1124-7 · 2.39 Impact Factor