S. Winter

Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswyck, Lower Saxony, Germany

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Publications (49)67.17 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Etiological studies of a recently emerged bushy top disease of tobacco in Ethiopia indicated that a ~4.5-kb dsRNA from infected plants represents an umbravirus, whereas a smaller band (~0.5 kb) is that of a new satellite RNA. Potato leafroll virus was also consistently associated with the disease. The three agents, whose experimental host ranges are restricted to members of the family Solanaceae, always occurred together in field samples and are transmitted together by the aphid Myzus persicae nicotianae. The umbravirus, which represents a new species, is most closely related to groundnut rosette virus, and the name Ethiopian tobacco bushy top virus is proposed.
    Archives of virology. 08/2014;
  • Phytopathologia Mediterranea 01/2014; 53(2):269-276. · 0.79 Impact Factor
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    ABSTRACT: During surveys conducted in 2012–2013, viruslike symptoms of chlorotic spots with, in some cases, a necrotic centre in older leaves were observed in field- and greenhouse-grown cucumber (Cucumis sativus L.), melon (C. melo L.) and squash (Cucurbita sp.) in the major cucurbit cultivation regions in Iran. Leaf samples were collected and tested for the presence of Cucumber leaf spot virus (CLSV, genus Aureusvirus, family Tombusviridae) by a virus specific double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). CLSV was detected in four of eight surveyed provinces in melon, cucumber and squash. When plant sap of ELISA positive samples was used to mechanically inoculate healthy squash plants, chlorotic spots with, in some cases, necrotic centres were observed on the inoculated leaves 20–25 days postinoculation. The presence of CLSV was confirmed by reverse transcription polymerase chain reactions using specific primers amplifying the entire coat protein gene of CLSV. Sequence comparison with sequences available at GenBank showed 93% nucleotide sequence identity to CLSV isolates from Israel (DQ227315) and Canada (EU127904), the only CLSV coat protein sequences available. To our knowledge, this is the first report of the occurrence of CLSV in Iran.
    Journal of Phytopathology 01/2014; 162:205-208. · 1.00 Impact Factor
  • Wulf Menzel, George Thottappilly, Stephan Winter
    Archives of Virology 10/2013; · 2.03 Impact Factor
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    ABSTRACT: The availability of characterised reference isolates of plant pathogens is of crucial importance for research and diagnostic laboratories. The Q-bank Plant Viruses and Viroids database contains data and information on plant viruses and viroids, with the unique feature that it is linked to specimens present in publicly available physical collections. The Q-bank database aims to share data and information on the virus and viroid species and their availability between research and diagnostic laboratories. Currently the database focuses on regulated virus species. In future more plant viruses and viroids will be included to provide a comprehensive data and information system. The curators invite virologists worldwide to participate in this international initiative by making their data and isolates available via Q-bank (http://www.q-bank.eu). La base de données et les collections Virus et Viroïdes des Plantes de Q-bank La disponibilité d'isolats de référence caractérisés est d'une importance cruciale pour les laboratoires de recherche et de diagnostic. La base de données de Q-bank sur les Virus et Viroïdes des plantes contient des données et des informations sur les virus et viroïdes, avec l'avantage d’être liée à des spécimens présents dans des collections physiques qui sont disponibles publiquement. La base de données Q-bank vise à permettre le partage de données et d'informations sur les espèces de virus et de viroïdes et leur mise à disposition entre laboratoires de recherche et de diagnostic. Actuellement, la base de données se concentre sur les espèces de virus réglementées. A l'avenir, d'autres virus et viroïdes des végétaux seront ajoutés pour fournir un système de données et d'informations cohérent et complet. Les curateurs invitent les virologistes dans le monde entier à participer à cette initiative internationale en rendant leurs données et leurs isolats disponibles au travers de Q-bank (http://www.q-bank.eu). Бaзa дaнныч о вирусaч и вироидaч рaстений и коллекции Q-bank Для иccлeдoвaтeльcкиx и диaгнocтичecкиx лaбopaтopий дocтyпнocть oxapaктepизoвaнныx cпpaвoчныx изoлятoв пaтoгeнoв pacтeний имeeт пepвocтeпeннoe знaчeниe. Бaзa дaнныx пoд нaзвaниeм «The Q-bank Plant Viruses & Viroids» coдepжит дaнныe и инфopмaцию o виpycax и виpoидax pacтeний и имeeт тy yникaльнyю ocoбeннocть, чтo oнa cвязaнa c экзeмпляpaми, нaличecтвyющими в пyбличнo дocтyпныx кoллeкцияx. Бaзa дaнныx Q-bank cтaвит cвoeй цeлью oбмeн дaнными и инфopмaциeй o видax виpycoв и виpoидax и иx дocтyпнocти для иccлeдoвaтeльcкиx и диaгнocтичecкиx лaбopaтopий. B нacтoящee вpeмя бaзa дaнныx cocpeдoтoчeнa нa peгyлиpyeмыx видax виpycoв. B бyдyщeм в нee бyдeт включeнo бoльшee кoличecтвo виpycoв и виpoидoв pacтeний, c тeм чтoбы oбecпeчивaлacь пoлнoтa дaнныx и инфopмaциoнныx cиcтeм. Кypaтopы этoй бaзы пpeдлaгaют виpoлoгaм вceгo миpa пoyчacтвoвaть в этoй мeждyнapoднoй инициaтивe пyтeм пpeдocтaвлeния cвoиx дaнныx и изoлятoв чepeз Q-bank (<http://www.q-bank.eu>).
    Bulletin OEPP/EPPO Bulletin 08/2013; 43(2).
  • Plant Disease 07/2013; 97(7):1005. · 2.74 Impact Factor
  • Archives of Virology 01/2012; · 2.03 Impact Factor
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    ABSTRACT: Export Date: 11 April 2012, Source: Scopus
    Journal of Phytopathology 01/2012; 160(3):163-165. · 1.00 Impact Factor
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    ABSTRACT: The complete genome of a tomato mild mottle virus (ToMMV) isolate was analysed, and some biological features were characterized. The ssRNA genome of ToMMV from Ethiopia encompasses 9283 nucleotides (excluding the 3' poly(A) tail) and encodes a polyprotein of 3011 amino acids. Phylogenetic and pairwise comparisons with other members of the family Potyviridae revealed that ToMMV is the most divergent member of the genus Ipomovirus, with a genome organization similar to that of members of the species Sweet potato mild mottle virus, the type species of the genus. In contrast to earlier reports, ToMMV isolates from Yemen and Ethiopia were not transmitted by the aphid Myzus persicae, but they were transmitted very erratically by the whitefly Bemisia tabaci. A comparison of the 3'-proximal sequences of different isolates provided evidence for geographically associated genetic variation.
    Archives of Virology 11/2011; 157(2):353-7. · 2.03 Impact Factor
  • Wulf Menzel, Mathew M Abang, Stephan Winter
    Archives of Virology 09/2011; 156(12):2309-11. · 2.03 Impact Factor
  • W Menzel, S Winter
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    ABSTRACT: In 1993, a virus causing red mosaic and leaf distortion has been isolated from black bryony (Tamus communis) in Italy. Based on particle properties and serology, the virus was assigned to the genus Potexvirus and named Tamus red mosaic virus (TRMV), pending a genome sequence. The original Italian TRMV isolate was submitted to the DSMZ plant virus collection (PV-0397). To confirm the taxonomic status of the virus, the entire genome sequence was determined comprising 6,495 nucleotides excluding the poly(A)-tail. Five putative open reading frames (ORFs) in an arrangement typical for potexviruses were predicted. TRMV is closely related to but distinct from Clover yellow mosaic virus and Allium virus X. In addition to previous morphological and serological characterization, the results presented in this study further reinforce the classification of TRMV as a distinct virus species of the genus Potexvirus.
    Virus Genes 09/2011; 44(1):120-3. · 1.77 Impact Factor
  • Archives of Virology 06/2011; 156(6):1107-10. · 2.03 Impact Factor
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    ABSTRACT: In vegetatively propagated Alstroemeria plants that showed pronounced stunting and necrotic leaf spots, a tobravirus infection was diagnosed in which one tobacco rattle virus (TRV, strain AL) RNA1 species was associated with seven different RNA2 species. The latter differed considerably in size and in the types of their 3' RNA1-related sequences. The 5' RNA2-specific part of all these RNA2 molecules showed almost 100% sequence identity with that of RNA2 of the TRV isolate TCM from tulip, but in some of these RNA2 molecules it was shorter than in the TCM isolate, whereas in others it was longer. One of the TRV AL RNA2 molecules, i.e. TC3'PE-a, contained the full set of three full-length RNA2-specific ORFs (ORF2a, -2b and -2c), whereas the previously analysed TCM sequence contained only ORF2a and -2b. In four of these TRV AL RNA2 molecules, i.e. those that had a relatively short RNA2-specific part, the 3' end was identical to that of the cognate TRV AL RNA1, but in the other three, which had a long RNA2-specific part, it was closely related to that of pea early browning virus (PEBV) RNA1, which was not detected in the infected plants. A comparison with previously described TRV/PEBV RNA2 recombinants suggested that the various TRV AL RNA2 molecules may represent various steps and side steps in an evolutionary process, which is apt to open the wide host range of TRV also to PEBV-derived RNA2 species.
    Journal of General Virology 12/2010; 92(Pt 4):988-96. · 3.13 Impact Factor
  • W Menzel, S Winter, H J Vetten
    Archives of Virology 10/2010; 155(12):2069-73. · 2.03 Impact Factor
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    ABSTRACT: Cassava brown streak virus (CBSV) isolates were analysed from symptomatic cassava collected between 1997 and 2008 in the major cultivation regions of East Africa. An analysis of complete RNA genomes of seven isolates from Kenya, Tanzania, Mozambique, Uganda and Malawi revealed a common genome structure, but the isolates clearly clustered in two distinct clades. The first comprised isolates from Kenya, Uganda, Malawi, north-western Tanzania and the CBSV described previously, and shared between 87 and 95% nucleotide sequence identity, whilst the second included isolates from coastal regions of Mozambique and Tanzania, which shared only 70% nucleotide sequence identities with isolates of the first clade. When the amino acid sequences of viral proteins were compared, identities as low as 47% (Ham1) and 59% (P1) between the two clades were found. An antiserum obtained against the capsid protein of a clade 1 isolate identified a 43 kDa protein in clade 1 isolates and a 45 kDa protein in clade 2 isolates. Several cassava cultivars were susceptible to isolates of clade 2 but resistant to those of clade 1. The differences observed both in biological behaviour and in genomic and protein sequences indicate that cassava brown streak disease in East Africa is caused by at least two distinct virus species. It is suggested that those of clade 1 retain the species name Cassava brown streak virus, whilst those of clade 2 be classified as Cassava brown streak Mozambique virus.
    Journal of General Virology 05/2010; 91(Pt 5):1365-72. · 3.13 Impact Factor
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    ABSTRACT: Export Date: 11 April 2012, Source: Scopus
    Plant Disease. 01/2010; 94(2):276.
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    ABSTRACT: Export Date: 11 April 2012, Source: Scopus
    Plant Disease. 01/2009; 93(11):1218.
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    M. A. Ali, S. Winter, G. A. Dafalla
    Plant Pathology 01/2009; 58(2):406-406. · 2.73 Impact Factor
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    ABSTRACT: Highly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of toxins in their pathogenesis. Secondary metabolite profiles in high performance tlc (hptlc) showed that the pathogenic SGG and FGS forms have a chemotype (A or B) that is distinct from the non-pathogenic FGG form (chemotype C). Crude extracts of 35-d-old Czapek-Dox yeast broth cultures of FGS and SGG isolates caused tissue necrosis on treated yam leaves but not those of FGG isolates. Extracts from uninoculated broth cultures showed no phytotoxic activity. Toxicity of the culture filtrate was not host specific and toxic substances were thermostable. Dioscorea genotypes with varying levels of resistance to anthracnose differed in their sensitivity to crude toxin extract of FGS (Cg33) and SGG (Cg25) isolates, indicating that these extracts may be useful in evaluating host resistance to anthracnose in vitro. Analysis of two toxin fractions unique to the pathogenic FGS and SGG forms using hlpc, mass spectrometry and nuclear magnetic resonance suggested the presence of a low molecular weight amide peptide. However, possibly due to low yield and the presence of impurities, the chemical structure of the compound(s) could not be fully elucidated.
    Mycological Research 10/2008; 113(Pt 1):130-40. · 2.81 Impact Factor
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    ABSTRACT: Abstract Two isolates of cherry leaf roll virus (CLRV), one from diseased beech and one from diseased birch trees in an area with forest decline near Bonn in West Germany, were found to be serologically closely related, but not indentical as assessed by spurformation of precipitin lines in agarose gel double diffusion tests. Such tests also distinguished these German CLRV isolates from ten other distinct CLRV isolates obtained from different natural hosts and from various countries. The German beech isolate was most similar serologically to isolates from walnut and from birch in England and the German birch isolate to an English cherry isolate and an isolate from Sambucus racemosa in Finland. These results provide further evidence of the antigenicdiversity of CLRV.ZusammenfassungSerologischer Vergleich von Isolaten des cherry leaf roll virus aus kranken Buchen und Birken in Waldschadensgebieten in Deutschland mit anderen Isolaten des VirusIsolate des cherry leaf roll virus (CLRV) aus kranken Rotbuchen und Birken in Waldschadensgebieten in Deutschland erwiesen sich serologisch im Agargeldoppeldiffusionstest als nahe verwandt, jedoch nicht als identisch. Mit einigen Antiseren kam es zur Spornbildung zwischen den Präzipitationslmien. Die beiden deutschen CLRV-Isolate konnten serologisch auch von zehn anderen CLRVIsolaren aus verschiedenen Ländern und von vcrschiedenen natürlichen Wirten unterschieden werden. Mit einigen auslandischen Isolaten waren die deutschen Isolate nahe verwandi. Eine besonders nahe Verwandtschaft wurde zwischen dem deurschen Buchenisoiat und Isolaten aus Walnuß und Birke aus England sowie dem deutschen Birkenisolai, einem englischen Kirschenisolai und dnem lsolat aus Sambucus racemosa aus FInnland festgestelk. Die Ergebnisse demonstrieren emeut die antigene Vielfalt von CLRV-Isolaten.
    Journal of Phytopathology 06/2008; 129(4):339 - 344. · 1.00 Impact Factor

Publication Stats

306 Citations
67.17 Total Impact Points

Institutions

  • 2002–2014
    • Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
      Brunswyck, Lower Saxony, Germany
  • 2010–2013
    • Julius Kühn-Institut
      Stadt Quedlinburg, Saxony-Anhalt, Germany
  • 2012
    • CBS Fungal Biodiversity Centre - Royal Netherlands Academy of Arts and Sciences
      Utrecht, Utrecht, Netherlands
  • 2008
    • Scottish Crop Research Institute
      Aberdeen, Scotland, United Kingdom
  • 2006
    • International Institute of Tropical Agriculture
      Ibadan, Oyo, Nigeria