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ABSTRACT: Our previous results showed that β-cyclodextrin-hemin complex (CDH) exhibited a vital protective role against cadmium-induced oxidative damage and toxicity in alfalfa seedling roots by the regulation of heme oxygenase-1 (HO-1) gene expression. In this report, we further test whether CDH exhibited the hormonal-like response. The application of CDH and an inducer of HO-1, hemin, were able to induce the up-regulation of cucumber HO-1 gene (CsHO1) expression and thereafter the promotion of adventitious rooting in cucumber explants. The effect is specific for HO-1 since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPP) blocked the above responses triggered by CDH, and the inhibitory effects were reversed further when 30% saturation of CO aqueous solution was added together. Further, molecular evidence showed that CDH triggered the increases of the HO-1-mediated target genes responsible for adventitious rooting, including one DnaJ-like gene (CsDNAJ-1) and two calcium-dependent protein kinase (CDPK) genes (CsCDPK1 and CsCDPK5), and were inhibited by ZnPP and reversed by CO. The calcium (Ca2+) chelator ethylene glycol-bis (2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) and the Ca2+ channel blocker lanthanum chloride (LaCl3) not only compromised the induction of adventitious rooting induced by CDH but also decreased the transcripts of above three target genes. However, the application of ascorbic acid (AsA), a well-known antioxidant in plants, failed to exhibit similar inducible effect on adventitious root formation. In short, above results illustrated that the response of CDH in the induction of cucumber adventitious rooting might be through HO-1-dependent mechanism and calcium signaling. KEY MESSAGE: Physiological, pharmacological and molecular evidence showed that β-cyclodextrin-hemin complex (CDH) was able to induce cucumber adventitious rooting through heme oxygenase-1 (HO-1)-dependent mechanism and calcium signaling.
Plant Cell Reports 04/2012; 31(9):1563-72. · 2.27 Impact Factor
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YANJIE XIE,
TENGFANG LING,
YI HAN,
KAILI LIU,
QINGSONG ZHENG, LIQIN HUANG,
XINGXING YUAN,
ZIYI HE,
BING HU,
LEI FANG,
ZHENGUO SHEN,
QING YANG,
WENBIAO SHEN
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ABSTRACT: Salt stress induced an increase in endogenous carbon monoxide (CO) production and the activity of the CO synthetic enzyme haem oxygenase (HO) in wheat seedling roots. In addition, a 50% CO aqueous solution, applied daily, not only resulted in the enhancement of CO release, but led to a significant reversal in dry weight (DW) and water loss caused by 150 mm NaCl treatment, which was mimicked by the application of two nitric oxide (NO) donors sodium nitroprusside (SNP) and diethylenetriamine NO adduct (DETA/NO). Further analyses showed that CO, as well as SNP, apparently up-regulated H+-pump and antioxidant enzyme activities or related transcripts, thus resulting in the increase of K/Na ratio and the alleviation of oxidative damage. Whereas, the CO/NO scavenger haemoglobin (Hb), NO scavenger or synthetic inhibitor methylene blue (MB) or NG-nitro-l-arginine methyl ester hydrochloride (l-NAME) differentially blocked these effects. Furthermore, CO was able to mimic the effect of SNP by strongly increasing NO release in the root tips, whereas the CO-induced NO signal was quenched by the addition of l-NAME or cPTIO, the specific scavenger of NO. The results suggested that CO might confer an increased tolerance to salinity stress by maintaining ion homeostasis and enhancing antioxidant system parameters in wheat seedling roots, both of which were partially mediated by NO signal.
Plant Cell and Environment 11/2008; 31(12):1864 - 1881. · 5.22 Impact Factor
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Yanjie Xie,
Tengfang Ling,
Yi Han,
Kaili Liu,
Qingsong Zheng, Liqin Huang,
Xingxing Yuan,
Ziyi He,
Bing Hu,
Lei Fang,
Zhenguo Shen,
Qing Yang,
Wenbiao Shen
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ABSTRACT: In the present work, we demonstrated for the first time that 150 mM NaCl treatment could induce the increase of endogenous carbon monoxide (CO) releasing in wheat seedling roots, the physiological significance of which was testified by exogenously CO application. We further discovered that exogenous application of 50% CO-saturated aqueous solution enhanced salt tolerance, and nitric oxide (NO) might be part of the downstream signal molecular of above CO action by the maintenance of ion homeostasis and up-regulation of antioxidant defense.
Plant Cell and Environment 06/2008; · 5.22 Impact Factor
