Karuppan Muthukumar

Anna University, Chennai, Chennai, Tamil Nādu, India

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Publications (45)119.31 Total impact

  • S R Vijayalakshmidevi, Karuppan Muthukumar
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    ABSTRACT: The present study demonstrates the de-colorization and degradation of textile effluent by coculture consisting of three bacterial species isolated from textile effluent contaminated environment with an aim to reduce the treatment time. The isolates were identified as Ochrobactrum sp., Pseudomonas aeruginosa and Providencia vermicola by 16S rRNA analysis. Their secondary structure was predicted and GC content of the sequence was found to be 54.39, 52.10, and 52.53%. The co-culture showed a prominent increase in the degradation activity due to the action of oxidoreductase enzymatic mechanism of laccase, NADH-DCIP reductase and azoreductase activity. The biodegradability index of 0.75 was achieved with 95% chemical oxygen demand (COD) reduction in 16h and 78 and 85% reduction in total organic carbon (TOC) and total solids was observed. Bioaccumulation of metals was identified by X-ray diffraction (XRD) analysis. The effective decolorization was confirmed from the results of UV-vis spectroscopy, high performance liquid chromatography and Fourier transformed infrared spectrometer analyzes. The possible degradation pathway was obtained from the analysis of liquid chromatography-mass spectroscopy analysis and the metabolites such as 2-amino naphthalene and N-phenyl-1.3,5 triazine were observed. The toxic nature of the effluent was analyzed using phyto-toxicity, cell-death assay and geno-toxicity tests. Copyright © 2014 Elsevier Inc. All rights reserved.
    Ecotoxicology and Environmental Safety 01/2015; 114C:23-30. · 2.20 Impact Factor
  • Govindarajan Ramadoss, Karuppan Muthukumar
    Chemical Engineering Journal 01/2015; 260:178–187. · 4.06 Impact Factor
  • Mujyambere Jean Marie Vianney, Karuppan Muthukumar
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    ABSTRACT: This study presents the removal of azo dyes (Congo red, Methyl red and Eriochrome black T) from aqueous solutions by ultrasound assisted electrocoagulation (UE). In this method, dye solutions were simultaneously sonicated and electrolyzed. The effect of various operating parameters such as intensity of irradiation, pH, current intensity, initial concentration of the dye and temperature on the performance of the process was investigated. The reduction in chemical oxygen demand, biological oxygen demand, hardness and alkalinity were assessed. The kinetic analysis of the data showed a pseudo-first order degradation for all three dyes. The results showed that UE is an effective method for the destruction of dyes.
    CLEAN - Soil Air Water 10/2014; · 2.05 Impact Factor
  • Balasubramani Ramprakash, Karuppan Muthukumar
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    ABSTRACT: This study presents the production of biohydrogen from rice mill wastewater. The acid hydrolysis and enzymatic hydrolysis operating conditions were optimized, for better reducing sugar production. The effect of pH and fermentation time on biohydrogen production from acid and enzymatic hydrolyzed rice mill wastewater was investigated, using Enterobacter aerogenes and Citrobacter ferundii. The enzymatic hydrolysis produced the maximum reducing sugar (15.8 g/L) compared to acid hydrolysis (14.2 g/L). The growth data obtained for E. aerogenes and C. ferundii, fitted well with the Logistic equation. The hydrogen yields of 1.74 mol H2/mol reducing sugar, and 1.40 mol H2/mol reducing sugar, were obtained from the hydrolyzate obtained from enzymatic and acid hydrolysis, respectively. The maximum hydrogen yield was obtained from E. aerogenes compared to C. ferundii, and the optimum pH for better hydrogen production was found to be in the range from 6.5 to 7.0. The chemical oxygen demand (COD) reduction obtained was around 71.8% after 60 h of fermentation.
    International Journal of Hydrogen Energy 09/2014; 39(27):14613–14621. · 2.93 Impact Factor
  • S.R. Vijayalakshmidevi, Karuppan Muthukumar
    Journal of Environmental Chemical Engineering 09/2014;
  • Zerihun Demrew Yigezu, Karuppan Muthukumar
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    ABSTRACT: This study presents the utilization of metal oxides for the biofuel production from vegetable oil. The physical and chemical properties of the diesel-like products obtained, and the influence of reaction variables on the product distribution were investigated. Six different metal oxides (Co3O4, KOH, MoO3, NiO, V2O5, and ZnO) were employed as catalysts and the results indicated that the metal oxides are suitable for catalyzing the conversion of oil into organic liquid products (OLPs). The maximum conversion (87.6%) was obtained with V2O5 at 320 °C in 40 min whereas a minimum conversion (55.1%) was obtained with MoO3 at 390 °C in 30 min. The physical characteristics of the product obtained (density, specific gravity, higher heat value, flash point and kinematic viscosity), were in line with ASTM D6751 (B100) standards. The hydrocarbons majorly present in the product were found to be methyl and ethyl esters. Furthermore, OLPs obtained were distilled and separated into four components. The amount of light hydrocarbons, gasoline, kerosene and heavy oil like components obtained were 18.73%, 33.62%, 24.91% and 90.93%, respectively.
    Energy Conversion and Management 08/2014; 84:326–333. · 3.59 Impact Factor
  • Source
    Arjunan Babuponnusami, Karuppan Muthukumar
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    ABSTRACT: The increase in the disposal of refractory organics demands for newer technologies for the complete mineralization of these wastewaters. Advanced oxidation processes (AOPs) constitute a promising technology for the treatment of such wastewaters and this study presents a general review on such processes developed to decolorize and/or degrade organic pollutants. Fundamentals and main applications of typical methods such as Fenton, electro-Fenton, photo-Fenton, sono-Fenton, sono-photo-Fenton, sono-electro-Fenton and photo-electro-Fenton are discussed. This review also highlights the application of nano-zero valent iron in treating refractory compounds.
    Journal of Environmental Chemical Engineering 03/2014; 2(1):557–572.
  • Senthilnathan Nachiappan, Karuppan Muthukumar
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    ABSTRACT: This study presents the treatment of a high strength pharmaceutical wastewater by sono-Fenton-sorption (SFS). Better results were observed with SFS compared to individual processes and the maximum chemical oxygen demand reduction observed after 180 min of treatment with SFS, sono-Fenton and sono-sorption was 71.9, 48.4, and 43.75%, respectively. The effect of parameters such as initial concentration, dosage of Fenton's reagent, sorbent dosage, and pattern of addition of iron on the performance of SFS was analyzed. The SS and SFS were found to fit well with Langmuir-Hinshelwood kinetics. Fourier transformed infrared studies of effluent and sorbent before and after treatment confirmed the oxidative degradation of pharmaceutical effluent. Toxicity of the effluent was considerably reduced after the treatment and the electrical energy consumption was significantly reduced, when sonolysis was coupled with sorption and Fenton's process (SFS).
    CLEAN - Soil Air Water 11/2013; 42(11). · 2.05 Impact Factor
  • S R Vijayalakshmidevi, Karuppan Muthukumar
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    ABSTRACT: This study presents the biodegradation of malachite green (MG), a triphenylmethane dye, using a novel microorganism isolated from textile effluent contaminated environment. The organism responsible for degradation was identified as Ochrobactrum sp JN214485 by 16S rRNA analysis. The effect of operating parameters such as temperature, pH, immobilized bead loading, and initial dye concentration on % degradation was studied, and their optimal values were found to be 30 °C, 6, 20 g/L and 100 mg/L, respectively. The analysis showed that the extracellular enzymes were responsible for the degradation. The biodegradation of MG was confirmed by UV-visible spectroscopic and FTIR analysis. The phytotoxicity test concluded that the degradation products were less toxic compared to MG. The kinetics of biodegradation was studied and the activation energy was found to be 10.65 kcal/mol.
    World Journal of Microbiology and Biotechnology (Formerly MIRCEN Journal of Applied Microbiology and Biotechnology) 08/2013; · 1.35 Impact Factor
  • Ramachandran Sivaramakrishnan, Karuppan Muthukumar
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    ABSTRACT: This work emphasizes the potential of the isolated Bacillus sp. lipase for the production of fatty acid methyl ester by the direct transesterification of Oedogonium sp. of macroalgae. Dimethyl carbonate was used as the extraction solvent and also as the reactant. The effect of solvent/algae ratio, water addition, catalyst, temperature, stirring and time on the direct transesterification was studied. The highest fatty acid methyl ester yield obtained under optimum conditions (5 g Oedogonium sp. powder, 7.5 ml of solvent (dimethyl carbonate)/g of algae, 8% catalyst (%wt/wt of oil), distilled water 1% (wt/wt of algae), 36 h, 55°C and 180 rpm) was 82%. Final product was subjected to thermogravimetric analysis and (1)H NMR analysis. The results showed that the isolated enzyme has good potential in catalyzing the direct transesterification of algae, and the dimethyl carbonate did not affect the activity of the isolated lipase.
    Journal of Bioscience and Bioengineering 07/2013; · 1.74 Impact Factor
  • Dharmaraj Gunasundari, Karuppan Muthukumar
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    ABSTRACT: This study presents simultaneous hexavalent chromium (Cr(VI)) reduction and phenol degradation using Stenotrophomonas sp., isolated from tannery effluent contaminated soil. Phenol was used as the sole carbon and energy source for Cr(VI) reduction. The optimization of different operating parameters was done using Placket-Burman design (PBD) and Box-Behnken design (BBD). The significant operating variables identified by PBD were initial Cr(VI) and phenol concentration, pH, temperature, and reaction time. These variables were optimized by a three-level BBD and the optimum initial Cr(VI) concentration, initial phenol concentration, pH, temperature, and reaction time obtained were 16.59 mg/l, 200.05 mg/l, 7.38, 31.96 °C and 4.07 days, respectively. Under the optimum conditions, 81.27 % Cr(VI) reduction and 100 % phenol degradation were observed experimentally. The results concluded that the Stenotrophomonas sp. could be used to decontaminate the effluents containing Cr(VI) and phenol effectively.
    Environmental Science and Pollution Research 04/2013; · 2.76 Impact Factor
  • Govindarajan Ramadoss, Karuppan Muthukumar
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    ABSTRACT: This study presents the ultrasound assisted ammonia pretreatment (UAAP) of sugarcane bagasse (SCB) and the influence of SCB particle size, liquid ammonia concentration, sonication time, temperature and liquid to solid ratio (LSR) on cellulose recovery and delignification. The maximum cellulose recovery and delignification observed at the optimum conditions (particle size 0.274 mm, sonication time 45 min, ammonia concentration 10%, LSR 10 mL/g and temperature 80 °C) were 95.78 and 58.14%, respectively. The dilute acid hydrolysis of pretreated SCB produced 16.58 g/L glucose, 8.21 g/L xylose, 2.78 g/L arabinose, 0.81 g/L furfural and 1.79 g/L acetic acid. The hydrolysate contained less inhibitors compared to the values reported in the literature during fermentable sugar production.
    Biochemical Engineering Journal 01/2013; · 2.37 Impact Factor
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    ABSTRACT: This study presents the degradation of phenanthrene by Mycoplana sp. MVMB2 isolated from petroleum contaminated soil and the media optimization by factorial design experiments. The Plackett–Burman design was used to evaluate the effects of eight variables (potassium dihydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, glucose, inoculum concentration, and phenanthrene concentration) on phenanthrene degradation. Based on the results, the critical medium components having significant influence on the degradation were found to be disodium hydrogen phosphate, magnesium sulfate, ferrous sulfate, and phenanthrene. Furthermore, these four variables were used as central composite design parameters. The optimum minimal salt medium composition obtained by conventional and factorial design experiments for the degradation of phenanthrene by Mycoplana sp. MVMB2 at pH 6.5 and 30°C were found to be, potassium 2.5 g/L dihydrogen phosphate, 0.3505 g/L disodium hydrogen phosphate, 0.5501 g/L magnesium sulfate, 0.02 g/L calcium chloride, 0.0261 g/L ferrous sulfate, 0.6756 g/L phenanthrene, 0.5 g/L glucose, 0.5 g/L ammonium sulfate, and inoculum 5% v/v. The phenanthrene degradation was confirmed by analyzing the metabolites formed.
    CLEAN - Soil Air Water 01/2013; 41(1). · 2.05 Impact Factor
  • Ramachandran Sivaramakrishnan, Karuppan Muthukumar
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    ABSTRACT: In this study, the production of methyl ester from Oedogonium sp. oil was studied using an isolated thermo-, solvent-, and sono-tolerant Bacillus sp. lipase immobilized on celite. The application of ultrasound during the reaction reduced the reaction time significantly. The effect of sonication time, enzyme dosage, water content, methanol/oil molar ratio, and solvent addition on the performance of transesterification was studied. The reaction time required in the presence and absence of ultrasound was 2 and 40 h, respectively. Under optimum conditions, 75 and 82% fatty acid methyl ester (FAME) yields were obtained for normal and ultrasound-assisted transesterification, respectively. The reusability of the immobilized enzyme after five cycles did not show much loss in enzyme activity, and this indicates that the isolated enzyme was not affected as a result of the application of ultrasound.
    Energy & Fuels 09/2012; 26(10):6387–6392. · 2.73 Impact Factor
  • Arjunan Babuponnusami, Karuppan Muthukumar
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    ABSTRACT: This study presents the removal of phenol by heterogenous photo electro Fenton-like (PEF-like) system using nano-zero valent iron (NZVI). NZVI was characterized using X-ray diffraction (XRD) and Scanning Electron Microscope (SEM) analysis. The effect of initial pH, initial concentration of phenol, NZVI dosage and initial H2O2 concentration on phenol removal was studied. The removal efficiency was increased with an increase in NZVI dosage and decreased with an increase in initial phenol concentration and initial pH. The optimum dosage of NZVI and H2O2 were found to be 0.5 g/L and 500 mg/L, respectively and complete removal of phenol was observed within 30 min at these conditions at an initial pH and initial phenol concentration of 6.2 and 200 mg/L, respectively. The analysis of kinetics showed that the removal of phenol by PEF-like process followed pseudo first-order kinetics. The variation of H2O2 concentration during the reaction was monitored at different current densities and the optimum current density was found to be 12 mA/cm2.
    Separation and Purification Technology 09/2012; 98:130–135. · 3.07 Impact Factor
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    ABSTRACT: This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30°C temperature, and 18.6 × 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study.
    CLEAN - Soil Air Water 08/2012; 40(8). · 2.05 Impact Factor
  • Arjunan Babuponnusami, Karuppan Muthukumar
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    ABSTRACT: This study presents the degradation of phenol by the photoelectro-Fenton method using nano zero-valent iron (nZVI) immobilized in polyvinyl alcohol-alginate beads. The effect of nZVI loading, H(2)O(2) concentration, pH, and initial phenol concentration on phenol degradation and chemical oxygen demand reduction was studied. The scanning electron microscope images of the nZVI beads were used to analyze their morphology, and their diameters were in the range of 500-600 μm. The concentration of nZVI in the beads was varied from 0.1 to 0.6 g/L. Fe(2+) leakage of 1 and 3 % was observed with 0.5 and 0.6 g/L of nZVI, respectively, and the observed beads' fracture frequency was 2 %, which confirmed the stability of the beads. The optimum operating conditions that arrived for better degradation were 0.5 g/L of nZVI, pH 6.2, and 400 mg H(2)O(2)/L. The treatment of effluent by this method increased the biodegradability index of the effluent, and the degradation data were found to follow pseudo first-order kinetics.
    Environmental Science and Pollution Research 06/2012; · 2.76 Impact Factor
  • Rajendran Velmurugan, Karuppan Muthukumar
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    ABSTRACT: This study presents the sono-assisted pretreatment and enzymatic saccharification of sugarcane bagasse (SCB) for the production of bioethanol. The effect of sono-assisted alkali (NaOH) pretreatment on the removal of hemicellulose and lignin from SCB was studied and the results showed 80.8% of hemicellulose and 90.6% of lignin removal. Sono-assisted enzymatic saccharification was performed with Cellulomonas flavigena (MTCC 7450) and the yield was found to be affected by liquid-to-solid ratio (LSR), cell mass and pH. The optimum reaction time, LSR, cell mass and pH were found to be 360 min, 15:1, 15 g/L and 6.0 respectively. At optimum conditions, the maximum glucose yield obtained was 91.28% of the theoretical yield and the maximum amount of glucose obtained was 38.4 g/L. The enhancement in performance may be correlated with the swelling of cellulose and accelerated enzymatic saccharification due to the application of ultrasound. The hydrolyzate obtained was fermented using Zymomonas mobilis (MTCC 89) and about 91.22% of the theoretical ethanol yield was observed in 36 h of fermentation.
    Biochemical Engineering Journal 04/2012; 63:1–9. · 2.37 Impact Factor
  • Rajendran Velmurugan, Karuppan Muthukumar
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    ABSTRACT: Ultrasound-assisted alkaline pretreatment of sugarcane bagasse (SCB) for fermentable sugar production was carried out and the influence of particle size, liquid to solid ratio (LSR), NaOH concentration, temperature and sonication time on delignification and reducing sugar production was ascertained with Placket-Burman design. The best combination of each significant factor was determined by a central composite design (CCD) and optimum pretreatment conditions for maximum reducing sugar yield (96.27%) were particle size of 0.27 mm, LSR of 25 ml/g, NaOH concentration of 2.89% (w/v), temperature of 70.15°C and pretreatment time of 47.42 min. Under these conditions, 92.11% of theoretical reducing sugar yield was observed experimentally. The substantial reduction in pretreatment time and temperature with improved efficiency is the most attractive features of the ultrasound-assisted alkaline pretreatment.
    Bioresource Technology 02/2012; 112:293-9. · 5.04 Impact Factor
  • Senthilnathan Nachiappan, Karuppan Muthukumar
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    ABSTRACT: In this study, sonolysis (US), a dual oxidant system (DOX), and ultrasound coupled with a dual oxidant system (US/DOX) were employed to degrade real pharmaceutical effluent. In a DOX system, two effective oxidizing agents such as hydrogen peroxide and activated persulphate were used simultaneously. In this work, for the first time, an easily available waste material, iron swarf, was used as an activator for persulphate oxidation. Iron swarf coupled with an ultrasound system showed better activation. High iron dosage, acidic pH and high temperature favoured degradation with both DOX and US/DOX. Sequential addition of iron swarf produced better results compared to single-step addition. The activation energy was found to be 47.25 and 23.47 kJ/mol for DOX and US/DOX, respectively. The biodegradability index of the effluent was enhanced from 0.13 to 0.81 after treatment with US/DOX.
    Environmental Technology 01/2012; 34(1-4):209-17. · 1.20 Impact Factor

Publication Stats

425 Citations
119.31 Total Impact Points

Institutions

  • 2004–2014
    • Anna University, Chennai
      • Department of Chemical Engineering
      Chennai, Tamil Nādu, India
  • 2013
    • Centro Universitário Sant’Anna
      San Paulo, São Paulo, Brazil
  • 2012
    • St. Joseph's College of Engineering, Chennai
      • Department of Chemical Engineering
      Chennai, Tamil Nādu, India
    • Adhiparasakthi Engineering College
      • Department of Chemical Engineering
      Kānchipuram, State of Tamil Nadu, India
  • 2004–2006
    • Alagappa Chettiar College of Engineering and Technology
      Karaikudi, Tamil Nādu, India