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Xiaohuan Guo, Yanfei Zhang,
Pingzhang Wang,
Ting Li,
Weiwei Fu,
Xiaoning Mo,
Taiping Shi,
Zhixin Zhang,
Yingyu Chen,
Dalong Ma,
Wenling Han
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ABSTRACT: Cytokines are soluble proteins that mediate immune reactions and are responsible for communication among immune cells. CD4(+) T cells are the principle sources of cytokines of adaptive immunity. Cytokines play critical roles in the differentiation and effector function of CD4(+) T cells. They also play key roles in diseases, and some of them have been developed into drugs in the forms of recombinant cytokines, soluble receptors and neutralizing antibodies. Therefore, identifying novel potential cytokines is necessary and beneficial for better understanding immunology and enhancing human health. To find novel potential cytokines, we carried out an integrated bioinformatics analysis on the whole human genome. Cytokine candidates were selected for cDNA cloning, sub-cloning, secretion verification, expression profile analysis and functional study. Here, we report a novel soluble protein, VSTM1-v2, which is a classical secretory glycoprotein mainly expressed in immune tissues, and can promote the differentiation and activation of Th17 cells.
Cellular Immunology 08/2012; 278(1-2):136-142. · 1.97 Impact Factor
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ABSTRACT: Perovskite-type SrZrO3 has been investigated as a candidate material for thermal barrier coating application. During plasma spraying of SrZrO3, SrO volatilized more than ZrO2 and the coating composition deviates from initial stoichiometry. In this investigation, partial evaporation was investigated
by spraying SrZrO3 powders into water. The influences of spraying current, distance and particle size of the powder on the partial evaporation
were also investigated in a quantitative way. With optimized spraying parameters, stoichiometric SrZrO3 coating was produced by adding an excess amount of Sr in the precursors before plasma spraying to compensate for the volatilized
component.
Journal of Thermal Spray Technology 04/2012; 18(4):694-701. · 1.81 Impact Factor
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ABSTRACT: Chemokine-like factor 1 (CKLF1) was the first member of the CKLF-like MARVEL transmembrane domain containing member (CMTM) family to be discovered. Its expression level is increased clearly in peripheral blood lymphocytes upon phytohemagglutinin stimulation, but little is known about the expression and function of CKLF1 in dendritic cells (DCs), which are the most potent antigen-presenting cells. In the present study, we showed that CKLF1 was highly expressed in monocytes. During differentiation from monocytes to immature DCs, CKLF1 was increased dramatically on day 2 and then decreased from day 3 to 5. Upon maturation with different stimuli, CKLF1 was down-regulated. Two peptides of CKLF1, C19 and C27, stimulated the effect of immature DCs (imDCs) on T-cell proliferation and IFN-gamma production. Further study on DC maturation showed that C19 and C27 up-regulated HLA-DR expression and IL-12 secretion, with no obvious effects on CD80, CD83 or CD86. Thus, CKLF1-C19 and -C27 can stimulate antigen-presenting capability of imDCs.
Cellular Immunology 03/2010; 263(2):188-95. · 1.97 Impact Factor
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ABSTRACT: One-dimensional SrAl(2)O(4):Eu(2+), Dy(3+) fibers were fabricated by a simple electrospinning combined with sol-gel process. X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and photoluminescence were used to characterize the fibers. The results show that the phase structure of SrAl(2)O(4):Eu(2+), Dy(3+) belongs to a monoclinic one, the composite fibers and fibers calcined at high temperature remain the original one-dimensional texture, and the SrAl(2)O(4):Eu(2+), Dy(3+) was a green emission.
Journal of Colloid and Interface Science 12/2009; 344(2):321-6. · 3.07 Impact Factor
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Berichte der deutschen chemischen Gesellschaft 08/2009; 2009(28):4248 - 4254. · 2.94 Impact Factor
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Chuanfei Yu,
Wenling Han,
Taiping Shi,
Bingfeng Lv,
Qihua He, Yanfei Zhang,
Ting Li,
Yingmei Zhang,
Quansheng Song,
Lu Wang,
Dalong Ma
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ABSTRACT: Cell migration plays a critical role during the development of most organisms and the process of malignant tumor metastasis. In the present study, we investigated the role of PTPIP51 (protein tyrosine phosphatase interacting protein 51) in cell motility. Overexpression of PTPIP51 induced cell elongation, increased cell migration, adhesion, and spreading, while downregulation of PTPIP51 had the opposite effects. We demonstrated here, that PTPIP51 could regulate ERK activity on Raf level, since MEK inhibitor and dominant-negative Raf-1 but not Ras could inhibit the ERK activation induced by PTPIP51. Further studies proved that PTPIP51 could interact with Raf-1 through 14-3-3, suggesting that PTPIP51 is a regulator of the Raf-MEK-ERK cascade through modulation of Raf-1 by 14-3-3. In addition, two redundant 14-3-3 binding domains in the PTPIP51 protein have been identified by deletion/mutation studies. We conclude that PTPIP51 regulates cell morphology and cell motility via interaction with Raf-1 through 14-3-3, and that PTPIP51 binds to 14-3-3 through two redundant binding domains.
Cellular signalling 09/2008; 20(12):2208-20. · 4.09 Impact Factor
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ABSTRACT: Nanowires of SiC were synthesized by carbothermally reducing PVP/TEOS composite fibres obtained by electrospinning. High-resolution transmission electron microscopy (HRTEM) and selected-area electron diffraction (SAED) indicated that the SiC nanowires are single crystalline in nature. Both Fourier-transformed infrared spectroscopy and HRTEM indicated that a thin layer of SiO2 was formed on the outer surface of the nanowire as a result of post-heat treatment for the removal of residual carbon. Such SiO2 layer protects the inner SiC fibre from further oxidation. The formation mechanism of single-crystalline SiC nanowires was proposed based on our understanding and characterizations. The growth of the nanowire is believed to be along the (111) of its cubic cell.
Nanotechnology 05/2007; 18(24):245606. · 3.98 Impact Factor
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ABSTRACT: The nanocrystals of CeF3 with the hexagonal structure and different morphologies such as the disk, the rod, and the dot have been successfully synthesized via a mild ultrasound assisted route from an aqueous solution of cerium nitrate and different fluorine sources (KBF4, NaF, NH4F). The use of different fluorine sources has a remarkable effect on the morphology of the final product. The luminescence and UV−vis absorption properties of CeF3 nanocrystals with different morphologies have been investigated. Compared with other shape nanocrystals, the luminescence intensity of the disklike nanocrystals is obviously enhanced. It is suggested that the function-improved materials could be obtained by tailoring the shape of the CeF3 nanocrystals.
04/2007;
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ABSTRACT: Electrospinning was employed to fabricate polymer-ceramic composite fibers from solutions containing poly(vinyl pyrrolidone) (PVP), Ce(NO(3))(3) x 6H(2)O and ZrOCl(2) x 8H(2)O. Upon firing the composite fibers at 1000 degrees C, Ce(0.67)Zr(0.33)O(2) fibers with diameters ranging from 0.4 to 2 microm were synthesized. These fibers exhibit strong resistance to sintering. They still have specific surface area around 11.8 m(2)/g after being heated at 1000 degrees C for 6 h.
Journal of Colloid and Interface Science 04/2007; 307(2):567-71. · 3.07 Impact Factor
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ABSTRACT: Lanthanum magnesium hexaalumminate (LMA) is an important candidate for thermal barrier coatings due to its thermal stability and low thermal conductivity. On the other hand, laser glazing method can potentially make thermal barrier coatings impermeable, resistant to corrosion on the surface and porous at bulk. LMA powder was synthesized at 1600 °C by solid-state reaction, pressed into tablet and laser glazed with a 5-kW continuous wave CO2 laser. Dendritic structures were observed on the surface of the laser-glazed specimen. The thicker the tablet, the easier the sample cracks. Cracking during laser glazing is attributed to the low thermal expansion coefficient and large thickness of the sample.
Optics and Lasers in Engineering.
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Journal of Colloid and Interface Science 307(2):567-571. · 3.07 Impact Factor
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ABSTRACT: A simple way to synthesize β-Ga2O3 nanoribbons and tubes by electrospinning is introduced. The diameters of the electrospun fibers range from 150 nm to 2.5 μm and their lengths reach up to several millimeters. The relationship among precursors, precursor concentrations, and crystal growth of β-Ga2O3 nanoribbons and tubes are discussed. The structures of β-Ga2O3 fibers have been investigated by various methods such as thermogravimetric (TG) and differential thermal analysis (DTA), X-ray diffraction, FT-IR, Raman spectra, scanning electron micrograph (SEM), and transmission electron micrograph (TEM).
Journal of Crystal Growth 308(1):180-184. · 1.73 Impact Factor
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ABSTRACT: Double-ceramic-layer (DCL) thermal barrier coatings (TBCs) of La2Zr2O7 (LZ) and yttria stabilized zirconia (YSZ) were deposited by electron beam-physical vapor deposition (EB-PVD). The composition, crystal structure, surface and cross-sectional morphologies and cyclic oxidation behavior of the DCL coating were studied. Both the X-ray diffraction (XRD) and thermogravimetric–differential thermal analysis (TG–DTA) prove that LZ and YSZ have good chemical applicability to form a DCL coating. The thermal cycling test at 1373 K in an air furnace indicates the DCL coating has a much longer lifetime than the single layer LZ coating, and even longer than that of the single layer YSZ coating. The failure of the DCL coating is a result of both the bond coat oxidation and the thermal strain between bond coat and ceramic layer generated by the thermal expansion mismatch. Additionally, the compressive stress initiated during cooling is also an important factor to control the cleavage of the interface between the LZ and YSZ coatings. Since no single material that has been studied so far satisfies all the requirements for high temperature TBCs, DCL coating is an important development direction of TBCs.
Journal of Alloys and Compounds 473:509-515. · 2.29 Impact Factor
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Xiaolong Chen, Yanfei Zhang,
Xinhua Zhong,
Zhenhua Xu,
Jiangfeng Zhang,
Yongliang Cheng,
Yu Zhao,
Yangjia Liu,
Xizhi Fan,
Ying Wang,
Hongmei Ma,
Xueqiang Cao
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ABSTRACT: Hexaluminates with magnetoplumbite structure are important high temperature ceramic materials for thermal barrier coatings (TBCs). Four hexaluminate coatings including NdMgAl11O19 (NdMA), SmMgAl11O19 (SmMA), GdMgAl11O19 (GdMA) and SrAl12O19 (SrHA) were prepared by plasma spraying. During plasma spraying, rare earth (RE) hexaluminates are partially decomposed, resulting in the loss of MgO. For RE hexaluminate coatings, the thermal cycling lifetime decreases with the reduction of RE3+ radius, while SrHA has the shortest lifetime. The random arrangement of the platelet-like hexagonal crystals resulted from the recrystallization during thermal cycling may reduce the bond strength of the coatings and make a large contribution to coatings’ failure. The different thermal cycling behaviors of these coatings seem to be dependent on their recrystallization differences to a large extent.
Journal of the European Ceramic Society.
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ABSTRACT: Nd2CexO3+2x (x = 2.25, 2.5, 2.75, 3.0) were synthesized by solid-state reaction, and their phase stabilities and thermophysical properties were investigated. The X-ray diffraction (XRD) results indicated that Nd2CexO3+2x with fluorite structure were stable after long-term annealing at 1673 K. They have higher thermal expansion coefficients (TECs) than yttria-stabilized zirconia (YSZ) which is the typical thermal barrier coating (TBC) material, especially the thermal expansion as a function of temperature is parallel to that of the nickel-based superalloy. This behavior is beneficial to the reduction of the residual stresses induced by the thermal expansion mismatch at the interface between the ceramic layer and bond coat. The reduction of sintering abilities of Nd2CexO3+2x coatings was achieved by the addition of excess CeO2 into the starting powders to make a nearly stoichiometric Nd2Ce2O7 coating. Nd2CexO3+2x can be explored as a new candidate material for TBCs.
Journal of Alloys and Compounds.
