Publications (2)3.35 Total impact
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ABSTRACT: The host mounts an immune response to pathogens, but few data are currently available on the role of host genetics in variation in response to avian influenza (AI). The study presented here investigated the role of the host genetic background in response to in vivo infection with AI virus (AIV). Experimental lines of chicken and commercial crosses were experimentally infected intratracheally with 103 EID50/bird of A/Chicken/Italy/13474/99 H7N1 highly pathogenic avian influenza virus (HPAIV). Chickens were genotyped for the Mx polymorphism causing the S631N mutation, and for the Major Histocompatibility Complex (MHC). Whole-genome genotyping was carried out using 60 k Single Nucleotide Polymorphism (SNP) array developed by the poultry Genome-Wide Marker-Assisted Selection Consortium (GWMASC). Variability in response of different chicken lines to the HPAIV infections and some degree of resistance to AI were observed: a statistically significant effect of chicken line on the response to infection was found. There was no association between survival in healthy conditions and polymorphisms at the Mx gene and the MHC-B region. The analysis based on the 60 k SNPs provided a good clustering of the chicken lines, but no specific genetic cluster associated with response to AIV was identified. Neither the genotype at the Mx gene or MHC-B locus, nor for SNP spanning the whole-genome identified loci involved in variations to response to AIV infection. These results point towards the possibility that either the genetic factors affecting the response of chickens to the H7N1 HPAIV are weak, or relevant alleles were not segregating in the studied populations.BMC proceedings 01/2011; 5 Suppl 4:S25.
Article: Susceptibility of different chicken lines to H7N1 highly pathogenic avian influenza virus and the role of Mx gene polymorphism coding amino acid position 631.[show abstract] [hide abstract]
ABSTRACT: Five chicken lines were experimentally infected with a HPAI H7N1 virus, to examine the variation in susceptibility to infection. Three lines showed high susceptibility to the virus, while two showed some resistance, with 7 out of 20, and 11 out of 15 birds, respectively, remaining healthy and surviving the experimental infection. Genotyping for the G/A polymorphism at position 2032 of Mx cDNA showed that one line was fixed for the G allele, and two were segregating for A and G alleles. Birds in the other two lines were selected to be fixed for the A allele. Statistical analyses indicated that the Mx genotype did not affect the clinical status or the time course of infection after viral inoculation.Virology 09/2008; 380(1):152-6. · 3.35 Impact Factor