[Show abstract][Hide abstract] ABSTRACT: A simple one-step method for preparing biocompatible nanoparticles of the gadolinium ferrocyanide coordination polymer KGd(H2O)2[Fe(CN)6]•H2O is reported. The crystal structure of this coordination polymer is determined by X-ray powder diffraction using the bulk materials. The stability, cytotoxicity, cellular uptake and MR phantom and cellular imaging studies suggest that this coordination-polymer structural platform offers a unique opportunity for developing the next generation of T1-weighted contrast agents with high relaxivity as cellular MR probes for biological receptors or markers to study molecular events as well as for in vivo MR imaging in biomedical research and clinical applications.
[Show abstract][Hide abstract] ABSTRACT: Nanoparticles of the title compound are prepared by slow mixing of an aqueous K4[Fe(CN)6] solution containing polyvinylpyrrolidone with an aqueous Bi(NO3)3 solution containing sodium citrate (room temperature, 30 min).
[Show abstract][Hide abstract] ABSTRACT: An aqueous synthetic procedure for preparing nanoparticles of the novel potassium bismuth ferrocyanide coordination polymer KBi(H(2)O)(2)[Fe(CN)(6)]·H(2)O is reported. The crystal structure of this coordination polymer is determined through X-ray powder diffraction using the bulk materials. The stability, cytotoxicity, and potential use of such nanoparticles coated with PVP as a CT contrast agent are investigated.
[Show abstract][Hide abstract] ABSTRACT: A simple and improved aqueous synthetic procedure using flash heating for preparing biocompatible Prussian blue nanoparticles (PBNPs) with the single-crystal-like feature was developed. This method entailed the use of citric acid as a chelator to complex ferric ions in the precursor and as an in situ capping agent to prevent the PB nanoparticles formed in solution from aggregation. The citrate-coated PBNPs, which can be surface functionalized with small molecules, were readily internalized by cells and exhibited no detectable cytotoxicity. Furthermore, such nanoparticles do not trigger the production of reactive oxygen species (ROS) in cellsvia the Fenton reaction. The potential of using such PBNPs as an effective T1-weighted cellular MRI contrast agent has also been demonstrated. These properties suggest that PBNPs are an attractive dual purpose nanoplatform for developing the next generation of nanoparticle-based T1-weighted MRI contrast agents as well as delivery vehicles for small molecules.
[Show abstract][Hide abstract] ABSTRACT: Author Summary
Breast cancer is one of the most frequently diagnosed malignancies in American females and is the second leading cause of cancer deaths in women. Several improvements in diagnostic protocols have enhanced our ability for earlier detection of breast cancer, resulting in improvement of therapeutic outcome and an increased survival rate for breast cancer patients. However, current early screening techniques are neither comprehensive nor infallible. Imaging techniques that improve breast cancer detection, localization, and evaluation of therapy are essential in combating the disease. Cysteine-rich intestinal protein 1 (CRIP1) has been identified as a novel marker for early detection of breast cancers. Here, we report the use of phage display and computational molecular modeling to identify a high-affinity ligand for CRIP1. Phage display panning experiments initially identified consensus peptide sequences with modest binding affinity to purified CRIP1. Using ab initio modeling of binding peptide structures, computational docking, and recently developed free energy estimation protocols, we redesigned the peptides to increase their affinity for CRIP1. Synthesis of the redesigned peptide and binding studies demonstrated approximately a 10–28-fold improvement in the binding affinity. The combination of computational and experimental techniques in this study demonstrates a potentially powerful tool in modulating protein–protein interactions.