Weijie Wang

Publications (1)0 Total impact

• Article: [Cloning and expression of the pig skeletal muscle musclin gene].

  Weijie Wang, Hongji Li, Liqiang Han, Yueying Wang, Jing Wang, Weihua Li, Maowang Lin, Yulei Tai, Zhiqiang Zhang, Meng Zang, Yanling Wang, Guoyu Yang
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  ABSTRACT: We found seven tag sequence with high homology in dbEST by using human musclin gene, and got its cDNA sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by RT-PCR, encoding 132 amino acid residue protein. The new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pGEX-4T-1, and the recombinant was transformed into E. coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 38.59 kD protein was successfully expressed in E. coli BL21 and identified by Western blotting.
  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 08/2008; 24(7):1248-52.