[Show abstract][Hide abstract] ABSTRACT: Objective: To report our experience with two tests, anti-cardiolipin antibody test [venereal
disease reasearch laboratory (VDRL) test] and specific treponemal test (Treponema pallidum
hemagglutination assay), used for screening antenatal, high risk cases and cases from sexually
transmitted infection in a tertiary care hospital from January 2006 till December 2008.
Methods: A total of 14639 samples received from various patient groups including antenatal
cases, patients attending sexually transmitted disease (STD) clinic, blood donors and HIV
positive patients were screened.
Results: Among the 14639 samples collected, 103 were positive by VDRL test. Of these 89
cases were confirmed by quantitative VDRL test and Treponema pallidum hemagglutination
assay. The cumulative seroprevalence over two years was found to be 0.61% in this study. The
syphilis seroprevalence reduced from 0.88% in 2006 to 0.40% in 2008. Among the various
sub-populations studied, patients attending the sexually transmitted infection clinic showed a
seroprevalence of 2.62%. The seroprevalence decreased significantly from 4.00% in 2006 to
1.39% in 2008.
Conclusions: Our study showed a statistically significant declining rate of syphilis in STD
clinics as well as the overall seroprevalence. These findings could be interpreted as indicators
of improved programmes for prevention and management of STDs.
Asian Pacific Journal of Tropical Biomedicine 11/2014; 4(12):995-997.
[Show abstract][Hide abstract] ABSTRACT: Delftia acidovorans (earlier known as Comamonas acidovorans) is an aerobic, non-fermentative, Gram negative rod, classified in the Pseudomonas rRNA homology Group III. Reports of isolation of the organism from serious infections like central venous catheter associated bacteremia, corneal ulcers, otitis media exist. The microbiologists can identify this organism based on an orange indole reaction. This reaction demonstrates the organism's ability to produce anthranilic acid from tryptophan on addition of Kovac's reagent; which gives the media its characteristic "pumpkin orange" colour. Here we report the isolation of this organism from the Endotracheal tube aspirate of a 4 year old child. With the increasing use of invasive devices, it has become important to recognize these non fermentative gram negative bacilli as emerging source of infection even in immunocompetent individuals.
Asian Pacific Journal of Tropical Biomedicine 11/2012; 2(11):923-4.
[Show abstract][Hide abstract] ABSTRACT: Background: Extended spectrum beta lactamase (ESBL)-producing Klebsiella pneumoniae have been occasionally reported as a cause of septicemia outbreak among pediatric patients in medical literature. We aimed to study the source of an outbreak of ESBL-producing K. pneumoniae in the Neonatal Intensive care Unit (NICU) at a tertiary care hospital in South India.Methods: The outbreak was investigated by phenotypically typing the isolates followed by random amplified polymorphic DNA analysis (RAPD). A total of 31 K. pneumoniae, consisting of 27 blood isolates from neonates and 4 environmental isolates were studied. Antibiotic susceptibility patterns were determined using standard disc diffusion methods; ESBL production was tested both phenotypically and genotypically. The strains were typed using two primers AP4 and HLWL74.Results: Except 2 environmental strains, all were found to be ESBL producers and of ESBL types TEM-1, SHV-12 and CTX-M- 15. Two different antibiotic resistance patterns were identified and the RAPD typing revealed two profiles. Phenotypic and genotypic analyses showed that 2 environmental strains had been responsible for the outbreak.Conclusion: Safe clinical practices should be followed in neonatal wards to prevent spread of infection. This is the first report of blaCTX-M-15 producing K.pneumoniae and the first outbreak in our hospital due to CTX-M-15 producing K.pneumoniae.
International Journal of Collaborative Research on Internal Medicine & Public Health. 01/2010;
[Show abstract][Hide abstract] ABSTRACT: Rapid diagnosis is prerequisite for effective treatment and reducing mortality and morbidity of malaria. This study was taken up to compare the efficacy of various methods available, i.e., thick and thin smear, quantitative buffy coat (QBC), plasmodium lactate dehydrogenase and aldolase in blood of patient. A total of 411 samples were collected from patients presenting with classic symptoms of malaria. For traditional microscopy; thick and thin smears were prepared and stained with Leishman's stain, taking thick smear as gold standard, thin smear had a sensitivity and specificity of 54.8% and 100%, respectively. QBC and antigen detection was done using commercially available kits; out of 411 samples, QBC and Malariagen were positive in 66 and 62 cases, with a sensitivity of 78% and 75%, respectively. Leishman's thick smear, although cost effective, is difficult to interpret for inexperienced microscopists; so if facilities are available, QBC should be used for routine diagnosis. In places where facilities are not available, rapid, simple and easy to interpret antigen detection test can be used despite low sensitivity.
Indian Journal of Pathology and Microbiology 01/2009; 52(2):200-2. · 0.64 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: ABSTRACT: INTRODUCTION : Melioidosis or infection with Burkholderia pseudomallei presents with protean manifestations. We present a case of melioidosis in a diabetic patient from India. The case is presented to highlight the importance of early microbiologic diagnosis and subsequent institution of appropriate therapy to achieve a better prognosis CASE PRESENTATION: A male bachelor around 50 years of age from India presented with low grade fever, bilateral ankle swelling and hypochondrial pain. On examination patient had diabetes and had multiple abscesses in bilateral ankle, knee and splenic region. Microbiologic diagnosis revealed the etiologic agent to be Burkholderia pseudomallei. Patient was managed with iv ceftazidime and surgical excision. CONCLUSION: The case report highlights the importance of early identification of etiologic agent. B. pseudomallei identification requires a great deal of clinical suspicion as well as alertness on the part of the medical microbiologist as these isolates are often reported as Pseudomonas spp. Correct identification of the etiologic agent is essential as B pseudomallei requires prolonged antimicrobial therapy for a better clinical outcome