Jinfeng Zhao

Publications (8)26.97 Total impact

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  Available from: Longxian Zhang

  Article: Cryptosporidium spp. in quails (Coturnix coturnix japonica) in Henan, China: molecular characterization and public health significance.

  Rongjun Wang, Fang Wang, Jinfeng Zhao, Meng Qi, Changshen Ning, Longxian Zhang, Lihua Xiao
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  ABSTRACT: The prevalence of Cryptosporidium spp. was investigated in scale quail (Coturnix coturnix japonica) farms in Henan Province, China between September 2006 and August 2007. One thousand eight hundred and eighteen fecal samples from 47 quail farms in five areas were collected for the examination of Cryptosporidium oocysts. The overall prevalence of Cryptosporidium was 13.1% (95% CI 13.1±1.6%) (29 of 47 farms), with 72-100-day-old quails having the highest prevalence (23.6%, 95% CI 23.6±2.6%) (χ(2)=64.91; ρ<0.01). The highest prevalence was observed in autumn (21.8%, 95% CI 21.8±3.1%) and the lowest in winter (χ(2)=74.83; ρ<0.01). Two hundred and thirty-nine Cryptosporidium-positive samples were analyzed by PCR-restriction fragment length polymorphism (RFLP) analysis of the small subunit (SSU) rRNA gene, and 42 were further analyzed by DNA sequencing of the PCR products. Two Cryptosporidium species were identified, Cryptosporidium baileyi in 237 birds on 29 farms, and potentially zoonotic Cryptosporidium meleagridis in only two birds on two farms. These findings may suggest that quails are not a major source of zoonotic Cryptosporidium in the study area.
  Veterinary Parasitology 02/2012; 187(3-4):534-7. · 2.58 Impact Factor
• Article: Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.

  Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning, Aiqin Liu, Jinfeng Zhao, Yaoyu Feng, Meng Qi, Helei Wang, Chaochao Lv, Guanghui Zhao, Lihua Xiao
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  ABSTRACT: In this study, nine C. muris and 43 C. andersoni isolates from various animals in China were subtyped by a multilocus sequence typing (MLST) tool. DNA sequence analyses showed the presence of 1-2 subtypes of C. muris and 2-6 subtypes of C. andersoni at each of the four loci (MS1, MS2, MS3, and MS16), nine of which represented new subtypes. Altogether, two C. muris and 10 C. andersoni MLST subtypes were detected. Linkage disequilibrium analysis indicated although the overall population structure of the two parasites was clonal, the Chinese C. andersoni in cattle has an epidemic structure. Three and two clusters were produced in the C. muris and C. andersoni populations by Structure 2.3.3 analysis, with Chinese C. muris and C. andersoni substructures differing from other countries. Thus, this study suggested the prevalence of C. andersoni in China is not attributed to the introduction of dairy cattle. More studies involving more genetic loci and systematic sampling are needed to better elucidate the population genetic structure of C. muris and C. andersoni in the world and the genetic basis for the difference in host specificity among the two most common gastric parasites.
  PLoS ONE 01/2012; 7(8):e43782. · 4.09 Impact Factor
• Article: Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.

  Huili Zhu, Jinfeng Zhao, Rongjun Wang, Longxian Zhang
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  ABSTRACT: Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.
  PLoS ONE 01/2012; 7(8):e43682. · 4.09 Impact Factor
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  Article: Prevalence and molecular characterization of Cyclospora cayetanensis, Henan, China.

  Yang Zhou, Biao Lv, Qiang Wang, Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning, Kanda Fu, Yaqiang Wang, Meng Qi, Huixia Yao, Jinfeng Zhao, Xiaoshan Zhang, Yanru Sun, Ke Shi, Michael J Arrowood, Lihua Xiao
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  ABSTRACT: To determine prevalence of Cyclospora cayetanensis infection in Henan, China, we conducted a study of 11,554 hospital patients. Prevalence was 0.70% (95% confidence interval 0.70% ± 0.15%), with all age groups infected. Most cases were found in the summer. Minor sequence polymorphisms were observed in the 18S rRNA gene of 35 isolates characterized.
  Emerging Infectious Diseases 10/2011; 17(10):1887-90. · 6.79 Impact Factor
• Article: Cryptosporidium tyzzeri n. sp. (Apicomplexa: Cryptosporidiidae) in domestic mice (Mus musculus).

  Xupeng Ren, Jinfeng Zhao, Longxian Zhang, Changshen Ning, Fuchun Jian, Rongjun Wang, Chaochao Lv, Qiang Wang, Michael J Arrowood, Lihua Xiao
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  ABSTRACT: The Cryptosporidium in the small intestine of domestic mice (Mus musculus) was initially described as Cryptosporidium parvum. Recent genetic and biologic characterization of Cryptosporidium isolates indicate that domestic mice are infected with several morphologically indistinguishable intestinal Cryptosporidium parasites with different host specificities, including C. parvum sensu stricto, mouse genotype I, and mouse genotype II. In this study, the morphological, biological, and genetic characteristics of the Cryptosporidium mouse genotype I are described. As a full re-description of C. parvum was made in 1985 for isolates from calves and humans and the name C. parvum has been widely used for the parasite that is infectious to both ruminants and humans, the mouse genotype I is named as Cryptosporidium tyzzeri. Oocysts of the new species (4.64±0.05 μm ×4.19±0.06 μm, with a mean shape index of 1.11±0.02; n=69) are slightly smaller than those of the re-described C. parvum. The prepatent period was six and seven days, and the patent period was 24-28 and 28-29 days in neonatal and adult mice, respectively. Oocysts were not infectious to lambs and calves. Light, transmission electron and scanning electron microscopy studies of the new species showed the presence of developmental stages in the microvillar brush border of the jejunum and ileum of experimentally infected mice, with the infection most intensive in the ileum. It had nucleotide sequences significantly different from C. parvum at the small subunit rRNA, 70 kDa heat shock protein, oocyst wall protein, actin, and the 60 kDa glycoprotein genes. Based on the morphological, genetic, and biological data and in compliance of established Cryptosporidium species naming criteria, this geographically widespread parasite is named as a new species in honor of Ernest Edward Tyzzer, who pioneered Cryptosporidium research.
  Experimental Parasitology 07/2011; 130(3):274-81. · 2.12 Impact Factor
• Article: Prevalence of Cryptosporidium baileyi in ostriches (Struthio camelus) in Zhengzhou, China.

  Rongjun Wang, Meng Qi, Zhu Jingjing, Dong Sun, Changshen Ning, Jinfeng Zhao, Longxian Zhang, Lihua Xiao
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  ABSTRACT: Few data are available on the molecular characterization of Cryptosporidium spp. in ostriches. The objective of this study was to determine the prevalence of Cryptosporidium species or genotypes in ostriches. A total of 452 fecal samples from five farms, a zoo, and an animal rescue center in Zhengzhou, Henan Province, China were examined for Cryptosporidium oocysts by microscopy of wet mount of fecal materials concentrated by the Sheather's sugar flotation technique. Fifty-three samples were Cryptosporidium-positive from four farms, with an overall prevalence of 11.7%. The percentage of animals shedding oocysts was 0, 16.2%, 7.2%, and 0 in 1-3 weeks, 4-8 weeks, 3-12 months, and more than 12 months ostriches, respectively (χ(2)=17.74; ρ<0.01). PCR-restriction fragment length polymorphism (RFLP) analysis of the small subunit (SSU) rRNA gene of the 53 Cryptosporidium-positive samples showed the presence of only Cryptosporidium baileyi, which was confirmed by DNA sequencing of the SSU rRNA PCR products from 16 positive samples. Cross-transmission studies demonstrated that the C. baileyi isolate could infect chickens and quails. Thus, ostriches are commonly infected with C. baileyi that is genetically and biologically similar to C. baileyi found in other birds.
  Veterinary Parasitology 10/2010; 175(1-2):151-4. · 2.58 Impact Factor
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  Article: Cryptosporidium andersoni is the predominant species in post-weaned and adult dairy cattle in China.

  Rongjun Wang, Guangpeng Ma, Jinfeng Zhao, Qingbin Lu, Helei Wang, Longxian Zhang, Fuchun Jian, Changshen Ning, Lihua Xiao
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  ABSTRACT: Dairy industry plays an important role in the agricultural economy of China. To estimate the prevalence and public health significance of cryptosporidiosis in post-weaned and adult dairy cattle in China, during four consecutive years (from 2006 to 2009), a total of 1315 fecal samples from 22 dairy cattle farms in ten prefectures in Henan Province were examined for the presence of Cryptosporidium oocysts. The overall prevalence of Cryptosporidium was 7.9%, with the highest infection rate (11.3%) in 3 to 11-month-old calves and the lowest infection rate (1.0%) in >2-year-old cows (p<0.01). Cryptosporidium-positive samples (n=104) were analyzed by PCR-restriction fragment length polymorphism (RFLP) analysis of the small subunit (SSU) rRNA gene, and 25 representative samples were further analyzed by DNA sequencing of the PCR products. Cryptosporidium bovis and Cryptosporidium andersoni were identified. C. andersoni (84/104) was the predominant species and was found in all age groups, whereas C. bovis (20/104) was only detected in 3 to 11-month-old calves. Thus, C. andersoni appears to be the dominant species in weaned dairy calves and heifers in China, in contrast with its common occurrence in adult cattle in other parts of the world.
  Parasitology International 09/2010; 60(1):1-4. · 2.13 Impact Factor
• Article: Molecular characterization of a new genotype of Cryptosporidium from American minks (Mustela vison) in China.

  Rongjun Wang, Longxian Zhang, Yaoyu Feng, Changshen Ning, Fuchun Jian, Lihua Xiao, Jinfeng Zhao, Yongli Wang
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  ABSTRACT: A total of 469 fecal samples were collected from American minks (Mustela vison) on a farm in Hebei Province in China and examined for Cryptosporidium by Sheather's sugar flotation technique and 8 Cryptosporidim isolates were obtained. The partial 18S rRNA, 70kDa heat shock protein (HSP70), Cryptosporidium oocyst wall protein (COWP) and actin genes of six isolates were sequenced. Sequence data were analyzed together with known Cryptosporidium spp. and genotypes. Results of this multi-locus genetic characterization indicated that the six Cryptosporidium isolates in this study shared the same sequences of the genes studied and were different from known Cryptosporidium species and genotypes. The closest relative was Cryptosporidium ferret genotype with 7, 22, 2 and 2 nucleotide differences in the 18S rRNA, HSP70, COWP and actin genes, respectively. The homology to ferret genotype at the 18S rRNA locus was 99.1%, which is comparable to that between C. parvum and C. hominis (99.2%), or between C. muris and C. andersoni (99.4%). Therefore, the Cryptosporidium in minks in this study is considered a new genotype, the Cryptosporidium mink genotype.
  Veterinary Parasitology 07/2008; 154(1-2):162-6. · 2.58 Impact Factor