Publications (6)11.9 Total impact
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Article: [Identification of metabolites of arbidol by ultra-high performance liquid chromatography tandem mass spectrometry].
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ABSTRACT: UPLC-MS-MS system was used for the identification of arbidol metabolites in the rat feces, urine and plasma samples. The system was so powerful a way with high ability of separation and analysis, based on both chromatography and mass properties. The isotope of Br was also a good indicator for metabolites finding. There were altogether 9 metabolites detected and identified, including 2 phase I biotransformation products and 7 phase II ones. It is concluded that arbidol mainly undergo metabolic reactions such as N-demethylation, S-oxidation, glucuronidation and sulfation in rats.Yao xue xue bao = Acta pharmaceutica Sinica 11/2012; 47(11):1521-6. -
Article: Cloud-Point Extraction Combined with LC–MS for Analysis of Memantine in Rat Plasma
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ABSTRACT: The feasibility of using cloud-point extraction as a simple and effective means of recovery of memantine from rat plasma before LC–MS analysis has been demonstrated. A non-ionic surfactant Triton X-114 was used for extraction of the memantine. On increasing the temperature to the cloud point, phase separation occurred, resulting in an aqueous phase, and a surfactant-rich phase containing most of the analytes. The extraction conditions, for example amount of surfactant, temperature, NaOH concentration, and time of incubation, were optimized. Chromatographic separation was accomplished on a C18 analytical column with 56:44 (v/v) methanol–0.2% aqueous formic acid as isocratic mobile phase at a flow rate of 0.8mLmin−1. Under the optimum experimental conditions recovery was satisfactory (91–101%) without interference from the surfactant. The method was shown to be reproducible and reliable with intraday precision below 6.6%, interday precision below 14.3%, and linear range from 1 to 400ngmL−1. The method was successfully applied to a pharmacokinetic study of memantine in rats after oral and intravenous administration.Chromatographia 04/2012; 69(9):837-842. · 1.20 Impact Factor -
Article: A sensitive liquid chromatography-mass spectrometry method for simultaneous determination of two active chromones from Saposhnikovia root in rat plasma and urine.
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ABSTRACT: A sensitive and efficient liquid chromatography-mass spectrometry method was developed and validated for the simultaneous determination of two active chromones (prim-O-glucosylcimifugin and 4'-O-D-glucosyl-5-O-methylvisamminol) from Saposhnikovia root in rat plasma and urine. The plasma or urine samples were prepared by protein precipitation. Chromatographic separation of the two active chromones from matrix interferences was achieved on an Angilent TC-C(18) column with a mobile phase consisted of methanol, water and 0.1% formic acid. Puerarin was added as the internal standard. The method was validated with the concentration range 1.0-100 ng/mL in rat plasma and 10-1000 ng/mL in urine for prim-O-glucosylcimifugin, 1.5-150 ng/mL in plasma and 15-1500 ng/mL in urine for 4'-O-D-glucosyl-5-O-methylvisamminol. The lower limit of quantitation (LLOQ) of prim-O-glucosylcimifugin and 4'-O-D-glucosyl-5-O-methylvisamminol was 1.0 and 1.5 ng/mL in plasma, 10 and 15 ng/mL in urine, respectively. The intra- and inter-day precision across three validation days over the entire concentration range was lower than 9.0% as terms of relative standard deviation (R.S.D.). Accuracy determined at three quality control concentrations (2.0, 25 and 75 ng/mL for prim-O-glucosylcimifugin; 3.0, 37.5 and 112.5 ng/mL for 4'-O-D-glucosyl-5-O-methylvisamminol) ranged from -1.9 to 3.9% as terms of relative error (R.E.). The LC-ESI-MS method was further applied to assess pharmacokinetics and urine excretion of the two chromones after oral administration of Fangfeng extract to rats. Practical utility of this new LC-MS method was confirmed in pilot pharmacokinetic studies in rats following oral administration.Journal of Chromatography B 07/2008; 868(1-2):13-9. · 2.89 Impact Factor -
Article: HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract.
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ABSTRACT: A simple HPLC method was developed for determination of quercitrin and isoquercitrin in rat plasma. Reversed-phase HPLC was employed for the quantitative analysis using kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnoside as an internal standard. Following extraction from the plasma samples with ethyl acetate-isopropanol (95:5, v/v), these two compounds were successfully separated on a Luna C(18) column (250 x 4.6 mm, 5 microm) with isocratic elution of acetonitrile-0.5% aqueous acetic acid (17:83, v/v) as the mobile phase. The flow-rate was set at 1 mL/min and the eluent was detected at 350 nm for both quercitrin and isoquercitrin. The method was linear over the studied ranges of 50-6000 and 50-5000 ng/mL for quercitrin and isoquercitrin, respectively. The intra- and inter-day precisions of the analysis were better than 13.1 and 13.2%, respectively. The lower limits of quantitation for quercitrin and isoquercitrin in plasma were both of 50 ng/mL. The mean extraction recoveries were 73 and 61% for quercitrin and isoquercitrin, respectively. The validated method was successfully applied to pharmacokinetic studies of the two analytes in rat plasma after the oral administration of Hypericum japonicum thunb. ethanol extract.Biomedical Chromatography 05/2008; 22(4):374-8. · 1.97 Impact Factor -
Article: Determination of arbidol in rat plasma by HPLC-UV using cloud-point extraction.
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ABSTRACT: A method based on cloud-point extraction (CPE) was developed to determine arbidol in rat plasma by high performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as the extract solvent. Variable parameters affecting the CPE efficiency were evaluated and optimized. A Zorbax SB-C(18) column (4.6 mm i.d. x 150 mm, 5 microm particle size) was used for isocratic elution separation at 40 degrees C with detection wavelength at 316 nm. Under the optimum conditions, the method was shown to be reproducible and reliable with intraday precision below 6.6%, interday precision below 8.8%, accuracy within +/-5.0% and mean extraction recovery more than 89.7%, which were all calculated using a range of spiked samples at three concentrations of 0.2, 2 and 16 microg/ml for arbidol in plasma. The linear range was from 0.08 to 20 microg/ml. After strict validation, the method was successfully applied to the pharmacokinetic study of arbidol in rats after oral and intravenous administration, respectively.Journal of Chromatography B 10/2007; 856(1-2):273-7. · 2.89 Impact Factor -
Article: Determination of arbidol in human plasma by LC-ESI-MS.
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ABSTRACT: A sensitive, specific and accurate method for determination of arbidol in human plasma was developed. Arbidol and internal standard were extracted from plasma samples by liquid-liquid extraction with diethyl ether. The chromatographic separation was accomplished on a Shiseido C18 3 microm analytical column (100 mm x 2.0 mm i.d.) at a flow rate of 0.3 mL/min isocratically. Detection was performed on a single quadrupole mass spectrometer by selected ion monitoring (SIM) mode via electrospray ionization (ESI) source. The method had a chromatographic run time of 6 min and a good linear relationship over the range 1-1000 ng/mL. The limit of quantitation for arbidol in plasma was 1 ng/mL. The intra-day and inter-day precision (R.S.D.%) was lower than 7% and accuracy ranged from 95 to 105%. The proposed method enables unambiguous identification and quantification of arbidol in vivo and has been successfully applied to study the pharmacokinetics of arbidol in healthy male Chinese volunteers.Journal of Pharmaceutical and Biomedical Analysis 02/2007; 43(1):371-5. · 2.97 Impact Factor
Top co-authors
Top Journals
Institutions
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2012
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Nanjing Medical University
Nanjing, Jiangsu Sheng, China
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2007–2012
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Shenyang Pharmaceutical University
- Department of Pharmacy
Shenyang, Liaoning, China
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