Jae-Hoon Kang

KAKEN Pharmaceutical Co.,Ltd, New York City, New York, United States

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Publications (11)9.02 Total impact

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    ABSTRACT: Abstract Context: The rhizome of Polygonatum sibiricum Redoute (Liliaceae) has long been used to treat diabetes-associated complications. However, the pharmacological mechanism of P. sibiricum on metabolic disorders is not clear. Objective: This study investigates the effect of an ethanol extract of P. sibiricum rhizomes (designated ID1216) on obesity conditions including weight loss in high-fat (HF) diet-fed mice and explores the potential underlying mechanisms. Methods: To identify the metabolic impact of the P. sibiricum rhizome extract, HF diet-fed mice were administered ID1216 orally at doses of 250 and 1000 mg/kg/d for 10 weeks, and various factors related to metabolic syndrome were analyzed. We also examined the effects of ID1216 on the expression of genes involved in adipogenesis and lipolysis in 3T3-L1 cells, as well as genes associated with energy homeostasis in C2C12 myocytes. Results: ID1216 administration led to significant decreases in body weight gain (37.5%), lipid accumulation in adipose tissues (52.8%), and the levels of plasma triglycerides (26.4%) and free fatty acids (28.1%) at a dose of 250 mg/kg/d, compared with the vehicle-treated group, as well as improved insulin resistance. In addition, ID1216 was found to regulate the expression of genes related to adipogenesis and fatty acid oxidation in 3T3-L1 cells and enhance the expression of genes that modulate energy homeostasis in C2C12 myocytes. Conclusions: ID1216 may be a promising therapeutic agent for improving obesity conditions through the sirtuin-1 and peroxisome proliferator-activated receptor γ coactivator-1α pathway.
    Pharmaceutical Biology 10/2014; · 1.21 Impact Factor
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    ABSTRACT: A previous study has demonstrated the anti-amyloidogenic effects of the ethanolic extract of Meliae Fructus (ID1201) using cell lines with stably expressed human Swedish mutant APP695 and β-secretase 1, and 5Xfamilial AD (FAD) mice carrying 5 mutations. Here, we investigated the effects of ID1201 on cognitive impairment in 5XFAD mice. Daily administration of ID1201 was commenced at 3 months of age and continued for 3 months. Mice were serially trained in cued/response and place/spatial training tasks in the Morris water maze. After this training, testing for strategy preference was conducted. Non-transgenic control mice with vehicle treatment, vehicle-treated 5XFAD, and ID1201-treated 5XFAD mice showed equivalent performance in cued/response training. However, as training progressed to the subsequent place/spatial learning, vehicle-treated control and ID1201-treated 5XFAD mice differed significantly from vehicle-treated 5XFAD mice in measures of spatial learning (search error and adaptive spatial learning strategy). In the strategy preference test that followed, control mice preferred a place/spatial strategy relative to vehicle-treated 5XFAD mice, but differences between ID1201-treated 5XFAD mice and vehicle-treated 5XFAD mice were not significant. Additionally, ID1201 treatment reduced hippocampal levels of insoluble Aβ42 and increased cortical levels of soluble amyloid precursor protein α. These results indicate that ID1201 may possess potential as a therapeutic agent for Alzheimer's disease by decreasing Aβ deposits.
    Neuroscience letters. 09/2014;
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    ABSTRACT: Amyloid beta (Aβ) peptides, which are generated from amyloid precursor protein (APP), are thought to play a major role in the pathogenesis of Alzheimer's disease (AD). This study investigated the anti-amyloidogenic effects of the ethanolic extract of Meliae Fructus (ID1201) using human embryonic kidney 293 cells with stably expressed human wild-type or Swedish mutant APP695 and β-secretase 1. ID1201 treatment enhanced the non-amyloidogenic metabolism of APP; increases in soluble APPα levels and decreases in soluble APPβ and Aβ levels resulted from the α-secretase activation through the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. In addition, ID1201-treated 5×familial AD (FAD) mice with 5 mutations in APP and presenilin 1 showed reduced levels of Aβ and amyloid plaques in the brain relative to those of 5×FAD mice with vehicle treatments. These results indicate that ID1201 possesses anti-amyloidogenic effects via the activation of the PI3K/Akt pathway, suggesting that it is a potential therapeutic agent for AD.
    Environmental toxicology and pharmacology. 01/2014; 37(2):513-520.
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    ABSTRACT: Probiotics and their products, such as yogurt and cheese have been widely consumed in many countries with proven health benefits including anti-microbial activity and anti-diarrheal activity. LHFM (Lactobacillus helveticus - fermented milk) is a processed skim milk powder, fermented by a probiotics, L. helveticus IDCC3801. In the present study, we aimed to investigate the neuroprotective effects and the cognitive improvements of LHFM. LHFM itself did not show any cytotoxicity to the human neuroblastoma cell line, SH-SY5Y; however, it dose-dependently protected against glutamate-induced neuronal cell death. LHFM also attenuated scopolamine-induced memory deficit in Y-maze and Morris-water maze. In the analysis of hippocampus after a behavior test, LHFM significantly increased the acetylcholine level and also inhibited acetylcholine esterase activity. Therefore, the raised acetylcholine release partially contributes to the improvement of learning and memory by a treatment with LHFM. These results suggest that LHFM is an effective material for prevention or improvement of cognitive impairments caused by neuronal cell damage and central cholinergic dysfunction.
    Korean Journal of Food Science and Technology. 08/2012; 44(4).
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    ABSTRACT: A Bacillus sp. that produces fibrinolytic enzyme was isolated from Cheonggukjang, a traditional Korean soybean-fermented food. According to 16S rRNA gene base sequencing, the bacillus was identified as a variety of Bacillus subtilis, and named Bacillus subtilis IDCC 9204. Fibrinolytic enzyme NK-IL9204 was stable up to and within pH range of 5-10. Purified NK-IL9204 was detected through fibrin zymography. The molecular weight and isoelectric point of the enzyme were estimated to be 27.7 kDa and 6.7 by SDS-PAGE and 2D electrophoresis, respectively. Its amino acid sequence was similar to that of nattokinase (identities 99.5%) and different from that of nattokinase BPN (identities 86.4%). The plasma fibrinolytic activity of NK-IL9204 was measured by euglobulin clot lysis times (ECLT). The NK-IL9204 was orally administered to SD rats for 3 weeks (1,000 FU/rat/day). The ECLT was significantly shortened by supplementation of NK-IL9204.
    Korean Journal of Food Science and Technology. 01/2012; 44(5).
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    ABSTRACT: In order to find new application fields for probiotics and their fermented products, we explored fermented milks cultured with various probiotic strains improving amyloid precursor protein (APP) metabolism in Alzheimer’s disease. In cell-based assay using wild type of APP and β-secretase over-expressing cell, the ethanol precipitates of the fermented milk cultured with Lactobacillus helveticus IDCC 3801, induced a strong decrease of APPβ level in amyloidogenic pathway toward β-amyloid production of APP processing. When administered orally to rat, the ethanol precipitate significantly reduced Aβ level in serum. In scopolamine-treated mouse model, the ethanol precipitate also attenuated memory deficit. Taken together, we suggest that the ethanol precipitate from fermented milk with L. helveticus IDCC3801 may improve APP metabolism and memory deficit.
    Journal of Functional Foods. 01/2010; 2(2):143-152.
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    ABSTRACT: Hyaluronic acid (HA), linear high-molecular-weight glycosaminoglycan produced from Streptococcus sp., has raised interest in the medical and cosmetics industries because of the various biological functions of HA. In this paper, we report on the optimization of medium components for HA production in Streptococcus sp. ID9102 (KCTC 11935BP) by two-step optimization (one-factor-at-a-time and taguchi orthogonal array design). In the first step, medium components, such as carbon, nitrogen, phosphate, and mineral sources, were selected for HA production in Streptococcus sp. ID9102 (KCTC 11935BP) using the one-factor-at-a-time method. In the second step, the concentration of the selected medium components was optimized using taguchi orthogonal array design. The design for medium optimization was developed and analyzed using MINITAB 14 software. In addition, the effect of amino acid and organic acid, such as glutamine, glutamate, and oxalic acid, was studied for HA production in Streptococcus sp. ID9102 (KCTC 11935BP). Through these processes, the optimum medium comprising 4% glucose, 0.75% yeast extract, 1.0% casein peptone, 0.25% K(2)HPO(4), 0.05% MgCl(2), 0.5% NaCl, 0.04% glutamine, 0.06% glutamate, and 0.02% oxalic acid was determined. We were able to produce HA with a molecular weight of 5.9 x 10(6) at a productivity of 6.94 g/l on pilot scale fermentation.
    Journal of Industrial Microbiology 08/2009; 36(11):1337-44. · 1.80 Impact Factor
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    ABSTRACT: The carbazole assay has been used for determination of the percentage of hyaluronic acid in biological fluids. However, it is difficult to measure the concentration of hyaluronic acid in culture broth because glucose and polysaccharides remaining after cultures can react with sulfuric acid and carbazole. The glucose and polysaccharide remnants must be completely removed in order to get the correct value for hyaluronic acid. The turbidity assay, another method for estimating the concentration of hyaluronic acid, is based on the formation of insoluble complexes between hyaluronic acid and cetyltrimethylammonium bromide. This method is very easy and fast compared with the carbazole assay. Because concentrations of hyaluronic acid measured by the turbidity assay were ranged around 100% of those measured by the carbazole assay, the content of hyaluronic acid in culture broth can be determined by the turbidity assay. The turbidity method also has the advantage of being safer than the carbazole assay.
    Carbohydrate Polymers 01/2009; 78(3):633-634. · 3.92 Impact Factor
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    ABSTRACT: We evaluated the influence of amino acids in improving teicoplanin productivity. Arginine, lysine, and proline were selected for better productivity among 20 amino acids in Erlenmeyer flasks. Proline was finally chosen as the additive for maximum teicoplanin productivity in a 5-liter fermenter. We obtained the highest teicoplanin productivity, 3.12 g/l, on the eighth d in a 75-liter pilot fermenter.
    Bioscience Biotechnology and Biochemistry 07/2008; 72(6):1635-7. · 1.27 Impact Factor
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    Bulletin- Korean Chemical Society 01/2006; 27(7). · 0.84 Impact Factor
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    ABSTRACT: Scheme 1. Reagents and conditions: (a) NH2OH·HCl, NaHCO3, EtOH; (b) Ac2O (for 5a and 5e) or (CF3CO)2O (for 5b and 5f) or ClC(= O) CO2Et (for 5c and 5g, and then NaBH4/THF), Pyridine, Toluene; (c) DAST, CH2Cl2. A number of life threatening infections caused by multidrug-resistant Gram-positive pathogens have reached an alarming level in the hospitals and the community. 1,2 Infections caused by these organisms pose a serious challenge to the scientific community and the need for an effective therapy has lead to a search for novel antibacterial agents. Oxazolidinones are a novel class of totally synthetic antimicrobial agents active against Gram-positive pathogenic bacteria including methicillin-resistant Staphylococcus aureus (MRSA), 3,4 vancomycin-resistant Ente-rococcus faecium (VRE), 5 and penicillin-resistant Streptoco-ccus pneumoniae (PRSP). 6,7 Linezolid (1) is the first member of this class currently launched worldwide, and available in both intravenous and oral formulations. Oxazolidinones have a unique mechanism for inhibiting bacterial protein synthesis at an early phase of translation by binding selectively to the central loop of domain of 23S rRNA of 50S ribosomal subunit. Oxazolidinones show no cross resistance with other classes of protein synthesis inhibitor. 8,9 Linezolid is efficacious in treating skin and soft tissue infections, pneumonia and bacteremia, and it is particularly effective against infections caused by MRSA, VRE, and PRSP. Recent studies by many research groups have focused on the development novel oxazolidinones based upon the replace-ment of the morpholine ring moiety of linezolid with various five-membered nitrogen-containing heterocycles (azoles).

Publication Stats

19 Citations
9.02 Total Impact Points

Institutions

  • 2012
    • KAKEN Pharmaceutical Co.,Ltd
      New York City, New York, United States
  • 2009
    • Jeil Pharmaceutical Co. Ltd
      Sŏul, Seoul, South Korea