Fangfang Chen

Publications (6)25.12 Total impact

• Article: Synthesis and photophysical properties of IrIII-LnIII (Ln = Nd, Yb, Er) bimetallic complexes containing bipyrimidines as bridging ligands

  FangFang Chen, WeiLi Jiang, Bin Lou, ZuQiang Bian, ChunHui Huang
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  ABSTRACT: Bipyrimidines have been chosen as (N∧N)(N∧N) bridging ligands for connecting metal centers. IrIII-LnIII (Ln = Nd, Yb, Er) bimetallic complexes [Ir(dfppy)2(μ-bpm)Ln(TTA)3]Cl were synthesized by using Ir(dfppy)2(bpm)Cl as the ligand coordinating to lanthanide complexes Ln(TTA)3·2H2O. The stability constants between Ir(dfppy)2(bpm)Cl and lanthanide ions were measured by fluorescence titration. The obvious quenching of visible emission from IrIII complex in the IrIII-LnIII (Ln = Nd, Yb, Er) bimetallic complexes indicates that energy transfer occurred from IrIII center to lanthanides. NIR emissions from NdIII, YbIII, and ErIII were obtained under the excitation of visible light by selective excitation of the IrIII-based chromophore. It was proven that Ir(dfppy)2(bpm)Cl as the ligand could effectively sensitize NIR emission from NdIII, YbIII, and ErIII.
  Science in China Series B Chemistry 04/2012; 52(11):1808-1813. · 1.20 Impact Factor
• Source

  Available from: yale.edu

  Article: Expression analysis of miRNAs and highly-expressed small RNAs in two rice subspecies and their reciprocal hybrids.

  Fangfang Chen, Guangming He, Hang He, Wei Chen, Xiaopeng Zhu, Manzhong Liang, Liangbi Chen, Xing Wang Deng
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  ABSTRACT: Heterosis, or hybrid vigor, is the phenomenon whereby progeny of two inbred lines exhibit superior agronomic performance compared with either parent. We analyzed the expression of miRNAs and highly expressed small RNAs (defined according to Solexa sequencing results) in two rice (Oryza sativa) subspecies (japonica cv. Nipponbare and indica cv. 93-11) and their reciprocal hybrids using microarrays. We found that of all the 1141 small RNAs tested, 140 (12%, 140 of 1141) and 157 (13%, 157 of 1141) were identified being significantly differentially expressed in two reciprocal hybrids, respectively. All possible modes of action, including additive, high- and low- parent, above high- and below low-parent modes were exhibited. Both F1 hybrids showed non-additive expression patterns, with downregulation predominating. Interestingly, 15 miRNAs displayed stark opposite expression trends relative to mid-parent in reciprocal hybrids. Computational prediction of targets of differentially expressed miRNAs showed that they participated in multifaceted developmental pathways, and were not distinguishable from the targets of non-differentially expressed miRNAs. Together, our findings reveal that small RNAs play roles in heterosis and add a new layer in the understanding and exploitation of molecular mechanisms of heterosis.
  Journal of Integrative Plant Biology 11/2010; 52(11):971-80. · 2.53 Impact Factor
• Article: Global epigenetic and transcriptional trends among two rice subspecies and their reciprocal hybrids.

  Guangming He, Xiaopeng Zhu, Axel A Elling, Liangbi Chen, Xiangfeng Wang, Lan Guo, Manzhong Liang, Hang He, Huiyong Zhang, Fangfang Chen, Yijun Qi, Runsheng Chen, Xing-Wang Deng
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  ABSTRACT: The behavior of transcriptomes and epigenomes in hybrids of heterotic parents is of fundamental interest. Here, we report highly integrated maps of the epigenome, mRNA, and small RNA transcriptomes of two rice (Oryza sativa) subspecies and their reciprocal hybrids. We found that gene activity was correlated with DNA methylation and both active and repressive histone modifications in transcribed regions. Differential epigenetic modifications correlated with changes in transcript levels among hybrids and parental lines. Distinct patterns in gene expression and epigenetic modifications in reciprocal hybrids were observed. Through analyses of single nucleotide polymorphisms from our sequence data, we observed a high correlation of allelic bias of epigenetic modifications or gene expression in reciprocal hybrids with their differences in the parental lines. The abundance of distinct small RNA size classes differed between the parents, and more small RNAs were downregulated than upregulated in the reciprocal hybrids. Together, our data reveal a comprehensive overview of transcriptional and epigenetic trends in heterotic rice crosses and provide a useful resource for the rice community.
  The Plant Cell 01/2010; 22(1):17-33. · 8.99 Impact Factor
• Article: Arabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 forms a nuclear E3 ubiquitin ligase with DDB1 and CUL4 that is involved in multiple plant developmental processes.

  Yu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang Deng
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  ABSTRACT: The human DDB1-CUL4 ASSOCIATED FACTOR (DCAF) proteins have been reported to interact directly with UV-DAMAGED DNA BINDING PROTEIN1 (DDB1) through the WDxR motif in their WD40 domain and function as substrate-recognition receptors for CULLIN4-based E3 ubiquitin ligases. Here, we identified and characterized a homolog of human DCAF1/VprBP in Arabidopsis thaliana. Yeast two-hybrid analysis demonstrated the physical interaction between DCAF1 and DDB1 from Arabidopsis, which is likely mediated via the WD40 domain of DCAF1 that contains two WDxR motifs. Moreover, coimmunoprecipitation assays showed that DCAF1 associates with DDB1, RELATED TO UBIQUITIN-modified CUL4, and the COP9 signalosome in vivo but not with CULLIN-ASSOCIATED and NEDDYLATION-DISSOCIATED1, CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), or the COP10-DET1-DDB1 complex, supporting the existence of a distinct Arabidopsis CUL4 E3 ubiquitin ligase, the CUL4-DDB1-DCAF1 complex. Transient expression of fluorescently tagged DCAF1, DDB1, and CUL4 in onion epidermal cells showed their colocalization in the nucleus, consistent with the notion that the CUL4-DDB1-DCAF1 complex functions as a nuclear E3 ubiquitin ligase. Genetic and phenotypic analysis of two T-DNA insertion mutants of DCAF1 showed that embryonic development of the dcaf1 homozygote is arrested at the globular stage, indicating that DCAF1 is essential for plant embryogenesis. Reducing the levels of DCAF1 leads to diverse developmental defects, implying that DCAF1 might be involved in multiple developmental pathways.
  The Plant Cell 07/2008; 20(6):1437-55. · 8.99 Impact Factor
• Article: Photophysical properties of heteroleptic iridium complexes containing carbazole-functionalized beta-diketonates.

  Zhiwei Liu, Daobo Nie, Zuqiang Bian, Fangfang Chen, Bin Lou, Jiang Bian, Chunhui Huang
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  ABSTRACT: Twelve iridium complexes with general formula of Ir(C;N)(2)(LX) [C;N represents the cyclometalated ligand, i.e. 2-(2,4-difluorophenyl) pyridine (dfppy), 2-phenylpyridine (ppy), dibenzo{f, h}quinoxaline (DBQ); LX stands for beta-diketonate, i.e. acetyl acetonate (acac), 1-(carbazol-9-yl)-5,5-dimethylhexane-2,4-diketonate (CBDK), 1-(carbazol-9-yl)-5,5,6,6,7,7,7-heptafluoroheptane-2,4-diketonate (CHFDK), 1-(N-ethyl-carbazol-3-yl)-4,4,5,5,6,6,6-heptafluorohexane-1,3-diketonate (ECHFDK)] are synthesized, characterized and their photophysical properties are systemically studied. In addition, crystals of Ir(DBQ)(2)(CHFDK) and Ir(DBQ)(2)(acac) are obtained and characterized by single crystal X-ray diffraction. The choice of these iridium complexes provides an opportunity for tracing the effect of the triplet energy level of ancillary ligands on the photophysical and electrochemical behaviors. Data show that if the triplet energy level of the beta-diketonate is higher than that of the Ir(C;N)(2) fragment and there is no superposition on the state density map, strong (3)LC or (3)MLCT-based phosphorescence can be obtained. Alternatively, if the state density map of the two parts are in superposition, the (3)LC or (3)MLCT-based transition will be quenched at room temperature. Density functional theory calculations show that these complexes can be divided into two categories. The lowest excited state is mainly determined by C;N but not beta-diketonate when the difference between the triplet energy levels of the two parts is large. However, when this difference is very small, the lowest excited state will be determined by both sides. This provides a satisfactory explanation for the experimental observations.
  ChemPhysChem 04/2008; 9(4):634-40. · 3.41 Impact Factor
• Article: Progresses in electroluminescence based on europium(III) complexes

  Fangfang CHEN, Zuqiang BIAN, Chunhui HUANG
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  ABSTRACT: The research on electroluminescence based on europium(III) complexes has come to an important phase. This article reviewed the progresses in photoluminescence and electroluminescence of Eu(III) complexes in recent years from the views of the design of Eu(III) complexes and optimization of device structures, and discussed some important factors influencing electroluminescence performance. The problems existing in the practical application such as the volatility and thermal stability of Eu(III) complexes in this area were discussed, and the possible corresponding solutions were briefly prospected.
  Journal of Rare Earths.