Zafer Kucukodaci

Publications (14)21.68 Total impact

• Article: Late features of fish vaccine injection injury: Case report

  Fatih Uygur, Rahmi Evinc, Yalcin Kulahci, Zafer Kucukodaci
  [show abstract] [hide abstract]
  ABSTRACT: Self-injection with fish vaccines is a rare occupational hazard for fish farm workers. Although there were some reports of early features of fish vaccine injuries, there were no reports about the late features of these injuries. A neglected fish farm worker who suffered from late term pathological features of an accidental injection injury of fish vaccine in finger region in reported.
  European Journal of Plastic Surgery 04/2012; 32(4):217-219.
• Article: Prevalence of the DNA repair enzyme-NEIL1 gene mutation in patients with Type 2 diabetes in the Turkish population.

  M Salmanoglu, Y Kucukardali, Z Kucukodaci, A Fenercioglu, E Solmazgul, Y Onem, H Baloglu, M Ozata
  [show abstract] [hide abstract]
  ABSTRACT: In spite of the fact that an indirect relationship between NEIL1 gene and Type 2 diabetes has been demonstrated in animal model studies, there have been no human studies showing this relationship. In our study, we aimed to show the relationship between NEIL1 mutation and Type 2 diabetes in humans. The study group consisted 70 patients with Type 2 diabetes and the control group consisted of 50 healthy individuals. The mean age was 53±11 yr and 49±11 yr, respectively. Two NEIL1 mutations (2.9%) were detected in the patient group. There was A→G change (133A→G) at the 133. position of the 8th exon with 257 bp length in base sequencing. There was no mutation in the control group. We searched NEIL1 gene mutation for the first time in patients with Type 2 diabetes. This mutation was "silent" as it did not cause any amino acid change. The effects of these mutations on the etiopathogenesis of disease are not known. Although the lysine encoded by AAG was identical to the lysine encoded by AAA, it is not clear if they have functional differences due to the changing environmental conditions. NEIL1 gene mutation may have causative role in the development of Type 2 diabetes.
  Journal of endocrinological investigation 10/2011; 35(4):401-6. · 1.57 Impact Factor
• Article: A valuable adjunct to FNA diagnosis of papillary thyroid carcinoma: in-house PCR assay for BRAF T1799A (V600E).

  Zafer Kucukodaci, Eylem Akar, Aptullah Haholu, Huseyin Baloglu
  [show abstract] [hide abstract]
  ABSTRACT: The diagnostic approach to thyroid nodules generally starts with FNA cytology. However, approximately one-fifth of cytologic evaluations yield indeterminate cytological findings but only 20% of cases with indeterminate thyroid nodule cytology have a cancer diagnosis, emphasizing the need for an effective ancillary test based on FNA material to help prevent unnecessary surgery. Detection of BRAFV600E mutation, the genetic signature of papillary thyroid carcinoma (PTC) in FNA material provides an invaluable diagnostic adjunct to overcome the limitations of FNA cytology. There are many ways to detect V600E, such as direct DNA sequencing, allele-specific PCR and hybridization-based colorimetric methods. In this study, a newer simple PCR method is presented that removes requirements for sequencing special equipment and commercial kits. Two forward primers including the mutant sequence specific (F2), and one common reverse (R) primer were optimized to generate a 241 bp fragment (F1R), an internal PCR control, and a 141 bp fragment (F2R) denoting the presence of V600E. Sensitivity studies revealed that the assay is capable of detecting V600E even in 1 ng of DNA. Direct sequencing data of 241 bp F1R fragment proved the specificity of the assay. For validation studies of the sequence specific multiplex PCR assay, archival FNA slides were used in a group of thyroid lesions including PTC, follicular carcinoma, follicular adenoma, Hashimoto thyroiditis, and benign thyroid nodules. The newer PCR-based method presented in this study is a practical, inexpensive one-step assay to detect the BRAF T1796A mutation on FNA samples.
  Diagnostic Cytopathology 06/2011; 39(6):424-7. · 1.16 Impact Factor
• Source

  Available from: dergisi.org

  Article: Existence of two separate facet joints on the same side: case of a congenital anomaly.

  Murat Kutlay, Berker Cemil, Kivanc Topuz, Mehmet Nusret Demircan, Zafer Kucukodaci
  [show abstract] [hide abstract]
  ABSTRACT: We present a case that had two separate facet joints on the same side causing an intervertebral instability. The embryological pathogenesis of the congenital existence of two separate facet joints on the same side of the vertebra is not conclusively known. A 68-year-old woman presented with lower back pain and severe left leg pain. Neuroradiological evaluation including dynamic plain radiography, computed tomography, and magnetic resonance imaging of the lumbar spine revealed the existence of two separate facet joints on the same side of the first sacral vertebra, severe degenerative changes of both right and left L5-S1 facet joints, and Grade II L5-S1 spondylolisthesis. Subsequently, she underwent surgery. Intraoperatively, two separate facet joints on the same side of the first sacral vertebra were confirmed. The patient's symptoms were resolved after decompression and fusion surgery. This is a unique case of the congenital existence of two separate facet joints on the same side of the first sacral vertebra.
  Turkish neurosurgery 01/2011; 21(2):239-41. · 0.62 Impact Factor
• Article: Combined hyperbaric oxygen and hypothermia treatment on oxidative stress parameters after spinal cord injury: an experimental study.

  Kivanc Topuz, Ahmet Colak, Berker Cemil, Murat Kutlay, Mehmet Nusret Demircan, Hakan Simsek, Osman Ipcioglu, Zafer Kucukodaci, Gunalp Uzun
  [show abstract] [hide abstract]
  ABSTRACT: We undertook this study to investigate the possible beneficial effects of combined hypothermia and hyperbaric oxygen (HBO) treatment in comparison with methylprednisolone in experimental spinal cord injury (SCI). Forty eight male Wistar albino rats (200-250 g) were randomized into six groups; A (normothermic control group; only laminectomy), B (normothermic trauma group; laminectomy + spinal trauma), C (normothermic methylprednisolone group; laminectomy + spinal trauma + methylprednisolone treated), D (hypothermia group; laminectomy + spinal trauma + hypothermia treated); E (HBO group; laminectomy + spinal trauma + HBO therapy), F (hypothermia and HBO group; laminectomy + spinal trauma + hypothermia and HBO treated) each containing eight rats. Neurological assessments were performed 24 h after trauma and spinal cord tissue samples had been harvested for both biochemical and histopathological evaluation. After SCI, tissue malondialdehyde (MDA) level of the control group was measured increased, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) enzyme activities were measured decreased. In group F, it was also shown that MDA level elevation had been prevented, and group F has increased the antioxidant enzyme activities than the other experimental groups C, D, E (p <0.05). We concluded that the use of combined hypothermia and HBO treatment might have potential benefits in spinal cord tissue on secondary damage.
  Archives of medical research 10/2010; 41(7):506-12. · 1.88 Impact Factor
• Article: Curcumin improves early functional results after experimental spinal cord injury.

  Berker Cemil, Kivanc Topuz, Mehmet Nusret Demircan, Gokhan Kurt, Kagan Tun, Murat Kutlay, Osman Ipcioglu, Zafer Kucukodaci
  [show abstract] [hide abstract]
  ABSTRACT: Curcumin is a polyphenol extracted from the rhizome of Curcuma longa and well known as a multifunctional drug with anti-oxidative, anticancerous, and anti-inflammatory activities. The aim of the study was to evaluate and compare the effects of the use of the curcumin and the methylprednisolone sodium succinate (MPSS) functionally, biochemically, and pathologically after experimental spinal cord injury (SCI). Forty rats were randomly allocated into five groups. Group 1 was performed only laminectomy. Group 2 was introduced 70-g closing force aneurysm clip injury. Group 3 was given 30 mg/kg MPSS intraperitoneally immediately after the trauma. Group 4 was given 200 mg/kg of curcumin immediately after the trauma. Group 5 was the vehicle, and immediately after trauma, 1 mL of rice bran oil was injected. The animals were examined by inclined plane score and Basso-Beattie-Bresnahan scale 24 h after the trauma. At the end of the experiment, spinal cord tissue samples were harvested to analyze tissue concentrations of malondialdehyde (MDA) levels, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activity, and catalase (CAT) activity and pathological evaluation. Curcumin treatment improved neurologic outcome, which was supported by decreased level of tissue MDA and increased levels of tissue GSH-Px, SOD, and CAT activity. Light microscopy results also showed preservation of tissue structure in the treatment group. This study showed the neuroprotective effects of curcumin on experimental SCI model. By increasing tissue levels of GSH-Px, SOD, and CAT, curcumin seems to reduce the effects of injury to the spinal cord, which may be beneficial for neuronal survival.
  Acta Neurochirurgica 09/2010; 152(9):1583-90; discussion 1590. · 1.52 Impact Factor
• Article: Human papillomavirus is not associated with breast carcinoma.

  Dilek Yavuzer, Taflan Salepci, Nimet Karadayi, Huseyin Baloglu, Zafer Kucukodaci
  Breast Cancer Research and Treatment 08/2010; 122(3):899-900. · 4.43 Impact Factor
• Article: Multiple recurrence of trichilemmal carcinoma of the scalp in a young adult.

  Yalcin Kulahci, Sinan Oksuz, Zafer Kucukodaci, Fatih Uygur, Ersin Ulkur
  [show abstract] [hide abstract]
  ABSTRACT: The authors have indicated no significant interest with commercial supporters.
  Dermatologic Surgery 02/2010; 36(4):551-4. · 1.80 Impact Factor
• Article: Preoperative imaging in renal masses: does size on computed tomography correlate with actual tumor size?

  Ferhat Ateş, Ilker Akyol, Onur Sildiroglu, Zafer Kucukodaci, Hasan Soydan, Kenan Karademir, Kadir Baykal
  [show abstract] [hide abstract]
  ABSTRACT: To evaluate the discrepancy between tumor sizes determined from preoperative computed tomography (CT) and surgical specimens and its clinical implications. The charts of 86 patients who underwent surgical resection of a renal mass between 1995 and 2007 were reviewed retrospectively. Tumor size was determined both from preoperative CT and pathologic specimen. Histopathologic evaluation was done. Wilcoxon test was used to compare the mean radiographic tumor size on CT with the mean pathologic size. P < 0.05 was considered as the threshold for statistical significance. The median age was 59 (21-84). Clinical stage was T1a in 13, T1b in 47, and ≥ T2 in 26; pathologic stage was T1a in 12, T1b in 45, and ≥ T2 in 29 patients. Histological subtype was clear cell, papillary, chromophobe, sarcomatoid, and oncocytic in 72, 7, 5, 1, and 1 patients, respectively. Mean radiographic and pathologic size was 6.33 and 6.43 cm, respectively (p = 0.342). On the average, radiographic measurement underestimated pathologic size by 1 mm. When subgroups of patients according to tumor size were formed as < 4, 4-7, and > 7 cm, mean radiographic size was 2.79, 5.44, and 9.57 cm, mean pathologic size was 3.47, 5.62, and 9.26 cm, respectively. In subgroups of < 4, 4-7, and > 7 cm; radiographic measurement underestimated pathologic size by 0.68 (p = 0.018) and 0.18 cm (p = 0.470) and overestimated by 0.31 cm (p = 0.454), respectively. Overall discrepancy between radiographic and pathologic tumor sizes was 1 mm. No significant stage shift due to measurement error was detected. Our findings suggest that CT is an accurate method with which to estimate renal tumor size preoperatively.
  International Urology and Nephrology 02/2010; 42(4):861-6. · 1.47 Impact Factor
• Article: Surgical treatment of facial disfigurement due to lichen myxedematosus.

  Cengiz Acikel, Huseyin Karagoz, Zafer Kucukodaci
  [show abstract] [hide abstract]
  ABSTRACT: The authors have indicated no significant interest with commercial supporters.
  Dermatologic Surgery 04/2009; 35(5):875-7. · 1.80 Impact Factor
• Article: Histologic analysis of the effects of three different support materials within rat middle ear.

  Salim Dogru, Abdulah Haholu, Atila Gungor, Zafer Kucukodaci, Hakan Cincik, Taner Ozdemir, Huseyin Sen
  [show abstract] [hide abstract]
  ABSTRACT: To investigate histologic changes in the mucosa of rat middle ear after implantation of three different support materials. A prospective, controlled animal study. Three types of absorbable materials were implanted into the middle ear cavity of rats: (1) Gelfoam (purified gelatin) (Pharmacia & Upjohn Company, New York, NY), (2) Sepragel (viscoelastic gel composed of cross-linked polymers of hyaluronan) (GENZYME Corp, Ridgefield, NJ), and (3) Nasopore (a biodegradable/fragmentable, synthetic polyurethane foam) (Polyganics, Groningen, The Netherlands). Rats were sacrificed after 3 and 20 days to ascertain early and late histologic changes. The bulla of each rat was excised and prepared for microscopic examination. The histologic changes were evaluated by observation of the middle ear cavity and mucosa in terms of polymorphonuclear leucocytes (PMNL), macrophages, giant cells, fibroblasts and other cells, fibrosis, and remnant materials. The histologic appearance of gelfoam-treated middle ears was characterized by more severe acute inflammation in the short-term and prominent fibrosis in the long-term in comparison with sepragel- and nasopore-treated groups. Nasopore appeared to be prone to remnant formation and reorganization by means of fibroblastic activity. Compared with gelfoam, both sepragel and nasopore caused less histologic alterations.
  Otolaryngology Head and Neck Surgery 02/2009; 140(2):177-82. · 1.72 Impact Factor
• Article: The effects of tissue fixation alternatives on DNA content: a study on normal colon tissue.

  Gulcan Baloglu, Aptullah Haholu, Zafer Kucukodaci, Ismail Yilmaz, Sukru Yildirim, Huseyin Baloglu
  [show abstract] [hide abstract]
  ABSTRACT: The aim of this study is to investigate the effects of different fixatives on DNA, and to evaluate the fixation options for molecular studies including polymerase chain reaction (PCR) and fluorescence in-situ hybridization (FISH). Three normal-looking colonic segments from surgical resections were used for tissue sampling. The full thickness of the colonic tissues (3 mm diameter) was sampled. Tissues were fixed in 70% ethanol, 10% neutral-buffered formalin, Hollande, B5, Bouin, and Zenker solutions for 1, 2, 5, 12, 24, and 48 hours, and processed and embedded in paraffin in a standard protocol. Quantitative measurements of the extracted DNA were carried out. DNA quality was tested by PCR for the human beta globin gene. Tissue sections were also tested for the availability of FISH, by using a Her-2/neu protocol. All fixation alternatives were found to be reasonable sources of DNA for molecular studies, and they enabled the successful PCR amplification of a housekeeping gene. DNA yields were predominantly over 1000 bp in 70% ethanol and 24-hour 10% neutral-buffered formalin fixations. As for B5 and Hollande, the DNA molecules obtained were almost all smaller than 100 bp. All tissues fixed in formalin, ethanol, and Hollande were found suitable for Her-2/neu visualization after standard FISH applications, but tissues fixed in Zenker, B5, and Bouin were not found suitable.
  Applied immunohistochemistry & molecular morphology: AIMM / official publication of the Society for Applied Immunohistochemistry 07/2008; 16(5):485-92. · 1.63 Impact Factor
• Article: The smooth muscle ratio at the renal pelvis in adults: does it predict surgical outcome?

  Temucin Senkul, Zafer Kucukodaci, Cuneyt Iseri, Kenan Karademir, Dogan Erden, Huseyin Baloglu, Yavuz Narin
  [show abstract] [hide abstract]
  ABSTRACT: Patients with ureteropelvic junction obstruction occasionally remain undiagnosed until adulthood. There are no objective criteria to predict the results of pyeloplasty in adult patients. We have evaluated the results of pyeloplasty in adult patients and investigated whether these results are correlated with the histopathology of the surgical specimen. Histological sections from 26 patients with ureteropelvic junction obstruction were analyzed. Their mean age was 25.1 years. Diethylenetriamine-pentaacetic acid scans were used to determine the degree of renal obstruction and postoperative healing. Improvement in diuretic renography was defined as a greater than 20% decrease in the half-time of the preoperative value. To examine smooth muscle and collagen tissue, sections were stained using Masson's trichrome. Smooth muscle ratio was identified by color image analysis. 18 patients (69.2%) fulfilled the criteria of healing. Patients with an improved scan had a mean smooth muscle percent (SMP) of 1.85+/-0.87 while subjects with no significant change in their diuretic scans had a mean SMP of 0.36+/-0.03 (p=0.001). There was a strong correlation between the SMP and the improvement. Adult pyeloplasty was found successful in about 70% of the cases. The SMP of the renal pelvis seems to be helpful in predicting the surgical outcome.
  Urologia Internationalis 01/2004; 73(3):248-51. · 0.99 Impact Factor
• Article: The comparison of the effects of different doses of levobupivacaine infiltration on wound healing.

  Kamer Dere, Huseyin Sen, Ertan Teksoz, Sezai Ozkan, Guner Dagli, Ilker Sucullu, Ali Ilker Filiz, Osman Metin Ipcioglu, Zafer Kucukodaci
  [show abstract] [hide abstract]
  ABSTRACT: INTRODUCTION-AIM: The easiest method in postoperative analgesia is the infiltration of the wound with local anesthetic drugs. Although many local anesthetic drugs have been used for this type of infiltration, studies on levobupivacaine are rare. The aim of this study was to investigate the effects of different concentrations of levobupivacaine infiltration on wound healing. Forty female Wistar-Albino rats (280-300 g) were included in the study, which were randomly separated into four groups. Rats were infiltrated with 1.25 mg/mL levobupivacaine in group L(1.25) (n = 10), with 2.50 mg/mL levobupivacaine in group L(2.5) (n = 10), with 3.75 mg/mL levobupivacaine in group L(3.75) (n = 10), and with normal saline in control group (n = 10). Breaking-strength measurements, levels of hydroxyproline, and fibrotic index were evaluated in the tissue samples taken from the rats. When the breaking-strength measurements were evaluated, we have found a significant difference between the control and the study groups (p < 0.05). In the intergroup comparison the difference between groups L(1.25) and L(3.75) was statistically significant (p < 0.05). In all of the levobupivacaine groups the levels of hydroxyproline were higher compared to the control group. Also significant differences were observed between groups L(1.25) and L(2.5) and groups L(1.25) and L(3.75) (p < 0.05). The levels of tissue fibrotic index were higher in all of the levobupivacaine groups compared to the control group (p < 0.05) and also a difference was observed between groups L(1.25) and L(3.75) in terms of tissue fibrotic index (p < 0.05). We have concluded that levobupivacaine used in clinical doses have a significant effect on the fastening of wound healing and this effect increases with an increase in the concentration of the levobupivacaine. We believe that levobupivacaine will be more widely preferred in the near future in the postoperative analgesia.
  Journal of Investigative Surgery 22(2):112-6. · 1.09 Impact Factor