[Show abstract][Hide abstract] ABSTRACT: Toll-like receptor 4 (TLR4) is known to mediate monocyte adhesion to endothelial cells, however, its role on the expression of monocyte adhesion molecules is unclear. In the present study, we investigated the role of TLR4 on the expression of monocyte adhesion molecules, and determined the functional role of TLR4-induced adhesion molecules on monocyte adhesion to endothelial cells. When THP-1 monocytes were stimulated with Kdo2-Lipid A (KLA), a specific TLR4 agonist, Mac-1 expression was markedly increased in association with an increased adhesion of monocytes to endothelial cells. These were attenuated by anti-Mac-1 antibody, suggesting a functional role of TLR4-induced Mac-1 on monocyte adhesion to endothelial cells. In monocytes treated with MK886, a 5-lipoxygenase (LO) inhibitor, both Mac-1 expression and monocyte adhesion to endothelial cells induced by KLA were markedly attenuated. Moreover, KLA increased the expression of mRNA and protein of 5-LO, suggesting a pivotal role of 5-LO on these processes. In in vivo studies, KLA increased monocyte adhesion to aortic endothelium of wild-type (WT) mice, which was attenuated in WT mice treated with anti-Mac-1 antibody as well as in TLR4-deficient mice. Taken together, TLR4-mediated expression of Mac-1 in monocytes plays a pivotal role on monocyte adhesion to vascular endothelium, leading to increased foam cell formation in the development of atherosclerosis.
PLoS ONE 08/2014; 9(8):e104588. · 3.53 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study aims to identify the factors related to resilience of the families of children with Down syndrome (DS). Data were collected from 126 parents of children with DS in Korea, using a self-administered questionnaire. The age of the child, developmental level of the child, parental depression, and stress and strain were negatively related with family adaptation, whereas health of parents, family cohesiveness, flexibility, communication skills, supportive family/relatives, and quality of community service were positively related. Parental depression, family cohesiveness and communication skills were the factors that were strongly related to family resilience and adaptation. It is suggested that nursing interventions to decrease parental depression and increase family cohesiveness and communication skills should be included to increase resilience of the families of children with DS.
International Journal of Nursing Practice 04/2014; · 0.54 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Deregulation of the PI3K-AKT/mTOR pathway due to mutation of the tumor suppressor gene PTEN frequently occurs in human prostate cancer and is therefore considered to be an attractive therapeutic target. Here, we investigated how the PTEN genotype affected the antitumor effect of NVP-BEZ235 in human prostate cancer cells. In this setting, NVP-BEZ235 induced cell death in a PTEN-independent manner. NVP-BEZ235 selectively induced apoptotic cell death in the prostate cancer cell line DU145, which harbors wild-type PTEN; however, in the PC3 cell line, which is PTEN-null, treatment with NVP-BEZ235 resulted in autophagic cell death. Consistently, NVP-BEZ235 treatment did not result in the cleavage of caspase-3; instead, it resulted in the conversion of LC3-I to LC3-II, indicating autophagic cell death; these results suggest that an alternate mechanism of cell death is induced by NVP-BEZ235 in PTEN-null prostate cancer cells. Based on our findings, we conclude that the PTEN/PI3K/Akt pathway is critical for prostate cancer survival, and targeting PI3K signaling by NVP-BEZ235 may be beneficial in the treatment of prostate cancer, independent of the PTEN genotype.
[Show abstract][Hide abstract] ABSTRACT: To assess the value of F-18 FDG PET/CT for detecting cervical lymph node (LN) metastasis and recurrence, as well as planning treatment, and to compare the accuracy of PET/CT with conventional imaging studies (CIS) in patients with malignant salivary gland tumor (SGT).
Nuclear medicine and molecular imaging. 12/2013; 47(4):242-248.
[Show abstract][Hide abstract] ABSTRACT: We report a facile approach to construct the hierarchically self-assembled dendron–cyclodextrin (CD) nanotubes with a dansyl fluorophore connected to the surface of the tube via a Gly-Gly-His (GGH) tripeptide spacer unit (Den-dans-GGH-CD-NTs) through a molecular recognition process of CDs and an amide dendron with the focal pyrene moiety. Den-dans-GGH-CD-NTs were utilized as a sensory platform for Cu(II) which can selectively bind to the GGH unit on the nanotube. Upon addition of Cu(II) into the tubular solution of Den-dans-GGH-CD-NTs, the fluorescence quenching of the dansyl fluorophore was observed due to photo-induced electron transfer to copper ions. A quantitative analysis showed that the detection limit for copper(II) ions was 148 nM. Furthermore, the Den-dans-GGH-CD-NT platform can be reused by abstraction of Cu(II) from the GGH unit with retaining the sensory capability.
New Journal of Chemistry 10/2013; 37(11). · 3.16 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: CBs News: Hierarchically self-assembled dendron nanotubes with a cucurbituril (CB) unit on the surface were constructed through self-assembly of CBs and an amide dendron with a focal pyene moiety. In addition, the self-assembled dendron-CB nanotubes were reversibly transformed into a vesicular architecture by removal of surface CB units from the dendron-CB nanotubes upon addition of Phe-Gly-Gly.
Chemistry - An Asian Journal 08/2013; · 4.57 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: AIMS: 5-Lipoxygenase (5-LO) is known to participate in the pathogenesis of atherosclerosis, however, the underlying mechanisms are unclear. Thus, this study investigated the molecular mechanisms responsible for 5-LO expression in monocytes as well as the role of 5-LO in monocyte adhesion to vascular endothelium, which is a key early event in macrophage foam cell formation.Methods and ResultsAn en face immunohistochemistry of endothelial surfaces revealed a marked increase in monocyte adhesion to aortic endothelium in wild type (WT) mice treated with LPS, which was significantly attenuated in 5-LO((-/-)) mice. Likewise, the adhesion capacity of primary monocytes isolated from LPS-treated WT mice was higher than those of monocytes from 5-LO((-/-)) mice. In in vitro study, LPS increased monocyte adhesion to endothelial cells with an enhanced Mac-1 expression. These were attenuated by a 5-LO inhibitor, MK886, as well as by molecular depletion of 5-LO in monocytes. Furthermore, LPS-induced Mac-1 expression on monocytes was significantly inhibited by pretreatment with U-75302, a BLT1-receptor antagonist, suggesting a pivotal role of 5-LO-derived leukotrienes. In promoter activity analysis and ChIP assays to identify transcription factors involved in 5-LO expression, both NF-κB and Sp1 played central roles to increase 5-LO expression in LPS-treated monocytes. CONCLUSION: 5-LO expression in monocytes is modulated via NF-κB and Sp1 signaling pathways, and 5-LO plays a pivotal role in LPS-mediated monocyte adhesion to vascular endothelium through an increased expression of Mac-1 on monocytes.
Cardiovascular Research 05/2013; · 5.81 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: PURPOSE: The aim of this study was to assess the value of dual-time point PET/CT in the differentiation of extrahepatic cholangiocarcinoma from benign stricture and the added benefits of delayed PET/CT image. PATIENTS AND METHODS: Thirty-nine patients with suspected extrahepatic biliary malignancy based on conventional imaging studies such as ultrasonography, CT, and MRI between July 2008 and December 2010 were included. All patients underwent dual-time point PET/CT scans at 1 and 2 hours after radiotracer injection. From the site of suspected malignancy, the SUVmax for both time points (SUVmax1 and SUVmax2), the percent change in SUVmax between the 2 (Δ%SUVmax) and the ratio of SUVmax1, SUVmax2, Δ%SUVmax in comparison with average SUV of right hepatic lobe (T/L ratio1, T/L ratio2, and Δ%T/L ratio) were generated. Diagnostic performances using visual assessment and various SUVmax cutoff values were analyzed in the differential diagnosis of extrahepatic cholangiocarcinoma from benign disease. PET/CT results were correlated with histological results and radiological follow-up for at least 6 months. RESULTS: Of 39 patients, the final diagnosis was cholangiocarcinoma in 34 and benign disease in 5. Between malignant and benign lesions, there were significant differences in SUVmax1 (5.43 ± 4.66 vs 2.26 ± 0.83, P = 0.003) and SUVmax2 (6.02 ± 5.26 vs 2.26 ± 0.76, P = 0.002). There was no significant difference in Δ%SUVmax, T/L ratio1, T/L ratio2, and Δ%T/L ratio. On receiver operator curve analysis, SUVmax1 with cutoff value of 2.5 demonstrated sensitivity of 97.6%, specificity of 60.0%, and accuracy of 92.3% and SUVmax2 with cutoff value of 3.1 demonstrated sensitivity of 88.2%, specificity of 100%, and accuracy of 89.7%. CONCLUSION: SUVmax from both early and delayed PET/CT scans are useful parameters in the differentiation of extrahepatic biliary malignancy from benign disease. However, there was no added benefit of delayed PET/CT in patients suspicious for extrahepatic cholangiocarcinoma.
Clinical nuclear medicine 01/2013; · 3.92 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: OBJECTIVE: Previous reports have suggested that the incidence of spina bifida occulta in patients with nocturnal enuresis is higher than in the general population. However, the effect of spina bifida occulta on the response to nocturnal enuresis treatment is controversial. The purpose of this study was to investigate the relationship between spina bifida occulta and response to treatment of nocturnal enuresis. METHODS: Between 2006 and 2009, the records of 160 children with nocturnal enuresis were reviewed. Children with other organic urological disease or symptoms suggestive of spinal dysraphism were excluded. Plain radiography for the kidney-ureter-bladder was carried out before the start of the nocturnal enuresis treatment. Response to treatment of children with and without spina bifida occulta was compared. RESULTS: Of 160 children, 53 were girls; the mean age was 7.8 ± 2.06 years. The mean duration of treatment was 8.7 ± 9.29 months. Spina bifida occulta was detected in 43 children (26.9%). Spina bifida occulta affected L4 in four children, L5 in 12 children, S1 in 26 children and S2 in one child. There was a significant difference between the spina bifida occulta and non-spina bifida occulta groups in terms of outcome (P = 0.002), with a complete response more likely in children without spina bifida occulta (P = 0.005). None of the children with primary non-mono symptomatic nocturnal enuresis and spina bifida occulta showed a complete response. CONCLUSIONS: The presence of spina bifida occulta significantly affects the response to treatment in patients with nocturnal enuresis. Thus, verifying spina bifida occulta in this patient population can facilitate the prediction of the response to nocturnal enuresis treatment.
International Journal of Urology 01/2013; · 1.80 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Objective
Incidental 18 F-FDG uptake in the ribs is a relatively common finding on PET/CT images of cancer patients. This study examined the role of 18 F-FDG PET/CT in differentiating between benign lesions and metastases on the ribs.
This study included 264 lesions in 172 PET/CT cases with underlying malignancy showing newly developed indeterminate 18 F-FDG rib uptake between June 2009 and May 2010. Patients with more than five FDG rib uptakes or hematologic malignancy were excluded. Malignancy was confirmed either histologically or by imaging studies, and clinical follow-up with serial images was at least 6 months. The maximum standardized uptake value (SUVmax) of the rib lesion was recorded. The FDG uptake patterns (focal or segmental; discrete or non-discrete), and CT findings (evidence of fracture, soft tissue lesions, osteoblastic and/or osteolytic lesions) were recorded.
There were 206 benign lesions and 58 metastases. The SUVmax was significantly higher in the metastatic group (3.0 ± 1.8) than in the benign group (2.5 ± 1.1), (p = 0.014). For the differential diagnosis between benign and metastatic lesions, the best SUVmax cut-off was determined to be 2.4. Significant indicators for metastasis were a segmental FDG uptake pattern (OR = 10.262, 95% CI 4.151 to 25.371), presence of an osteoblastic/-lytic lesion (OR = 22.903, 95% CI 10.468 to 50.108) and the absence of fractures on CT (OR = 291.629, 95% CI 39.09-2175.666).
SUVmax alone is not sufficient to differentiate benign and metastatic rib lesions in cancer patients. The diagnostic accuracy can be further increased when findings of the CT part of PET/CT are considered.
[Show abstract][Hide abstract] ABSTRACT: OBJECTIVE: Intravesical electrical stimulation (IVES) has been performed for various purposes in children with a neurogenic bladder. We evaluated the results of IVES on urodynamic study parameters in children with spina bifida. METHODS: We retrospectively analyzed the cases of 88 children who received IVES between August 1999 and May 2010 and whose comparative urodynamic data were available before and after treatment. According to the pre-IVES urodynamic study, children were divided into 3 groups: detrusor overactivity, detrusor underactivity and acontractile detrusor. We investigated the changes in detrusor function, bladder capacity and detrusor-sphincter dyssynergia. RESULTS: In the group showing detrusor overactivity, the bladder had a synergic pattern in 41.7%, and normal detrusor function was observed in 16.7% of them. Bladder capacity increased after IVES therapy, especially in those who started therapy before 18 months of age (p = 0.019). Detrusor-sphincter dyssynergia was resolved in 55.6%. In the acontractile detrusor group, detrusor contraction ability increased in 48%, but bladder capacity did not. CONCLUSIONS: Appropriate candidates for this treatment need to be carefully selected.
Journal of pediatric urology 11/2012; · 1.38 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Purpose
To assess the value of PET/CT for detecting local or distant recurrence in patients who undergo surgery for colorectal cancer (CRC) and to compare the accuracy of PET/CT to that of conventional imaging studies (CIS).
Tumor surveillance PET/CT scans done between March 2005 and December 2009 of disease-free patients after surgery with or without adjuvant chemotherapy for CRC were retrospectively studied. CIS (serial enhanced CT from lung base to pelvis and plain chest radiograph) were performed within 1 month of PET/CT. We excluded patients with distant metastasis on initial staging, a known recurrent tumor, and a lack of follow-up imaging. The final diagnosis was based on at least 6 months of follow-up with colonoscopy, biopsy, and serial imaging studies in combination with carcinoembryonic antigen levels.
A total of 262 PET/CT scans of 245 patients were included. Local and distant recurrences were detected in 27 cases (10.3%). On case-based analysis, the overall sensitivity, specificity, and accuracy were 100, 97.0, and 97.3% for PET/CT and 85.1, 97.0, and 95.8% for CIS, respectively. On lesion-based analysis, PET/CT detected more lesions compared to CIS in local recurrence and lung metastasis. PET/CT and CIS detected the same number of lesions in abdominal lymph nodes, hepatic metastasis, and peritoneal carcinomatosis. PET/CT detected two more metachronous tumors than did CIS in the lung and thyroid gland.
PET/CT detected more recurrences in patients who underwent surgery for CRC than did CIS and had the additional advantage of evaluating the entire body during a single scan.
Nuclear Medicine and Molecular Imaging. 09/2012; 46(3).
[Show abstract][Hide abstract] ABSTRACT: We report on the dual function of a silica–iron oxide hybrid nanoparticle combined with a stimulus responsive gatekeeper on the surface. Multifunctional hybrid nanoparticles (Fe@Si–DOX–CD–PEG) composed of an Fe3O4 core, a mesoporous silica shell, and GSH-responsive CD gatekeepers are an excellent platform for anticancer drug delivery and MR imaging. In particular, the CD gatekeepers on the surface of the hybrid nanoparticle play a key role in accommodating anticancer drug molecules in the pore of the silica shell without premature release until CD gatekeepers are cleaved by GSH. We confirmed, from an in vitro study with the A549 cell line, that DOX was released from the internalized carriers due to GSH-mediated cleavage of the CD gatekeeper. Consequently, apoptotic and clonogenic death occurred in the cells treated with Fe@Si–DOX–CD–PEG. The accumulation of Fe@Si–DOX–CD–PEG in the tumors was detected by in vivo MR imaging. The growth of the tumor in vivo was effectively suppressed by the intravenously injected Fe@Si–DOX–CD–PEG. In addition, the results of the in vivo MR imaging reflected the in vivo inhibition of the cancer growth by Fe@Si–DOX–CD–PEG.
Journal of Materials Chemistry 06/2012; 22(28):14061-14067. · 6.63 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A 51-year-old man was diagnosed with small cell lung cancer (SCLC) with brain, left adrenal gland, and pancreas metastases. On evaluation of treatment response after chemotherapy and radiation therapy, F-18 FDG PET/CT showed focal FDG uptake in the periappendiceal area with thickened appendiceal wall and inflammatory change. Laparoscopic appendectomy was done, and the findings were consistent with acute suppurative appendicitis with involvement of 2 small masses on the appendiceal body and tip. Histopathologic findings confirmed metastatic small cell carcinoma, involving submucosa, proper muscle, and periappendiceal fat. This is a case of appendiceal metastasis from small cell lung cancer detected on F-18 FDG PET/CT.
Clinical nuclear medicine 01/2012; 37(1):e19-21. · 3.92 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Purpose
This study aims to examine the findings of 99mTc-diphosphonate bone scans in cancer patients with a history of HIFU treatment.
Bone scan images of patients with a history of HIFU treatment for primary or metastatic cancer from January 2006 to July 2010 were retrospectively reviewed. Cases of primary bone tumor or HIFU treatment reaching only the superficial soft tissue layer were excluded.
Bone scan images of 62 patients (26 female, 36 male; mean age 57 ± 9 years) were studied. HIFU treatment was performed in the liver (n = 40), pancreas (n = 16), and breast (n = 6). Mean interval time between HIFU treatment and bone scan was 106 ± 105 days (range: 1–572 days). Of 62 scans, 43 showed diffusely decreased uptake of bone within the path of HIFU treatment: antero-axillary and/or posterior arcs of right 5th to 11th ribs in 34 cases after treatment of hepatic lesions; anterior arcs of 2nd to 5th ribs in 5 cases after treatment for breast tumors; and posterior arcs of left 9th to 11th ribs or thoraco-lumbar vertebrae in 4 cases after treatment for pancreas tumor. Of 20 patients who had bone scans more than twice, five showed recovered uptake of the radiotracer in the involved ribs in the follow-up bone scan.
Of 62 bone scans in patients with a history of HIFU treatment for primary or metastatic cancer, 69% presented diffusely decreased uptake in the bone in the path of HIFU treatment.
Nuclear Medicine and Molecular Imaging. 12/2011; 45(4).
[Show abstract][Hide abstract] ABSTRACT: The purpose is to describe factors associated with emotional responses of parents at the time of diagnosis of Down syndrome.
Using a survey design, data on attitudes of healthcare providers, content of information, and emotional responses were collected from 131 parents in Korea.
There were positive relationships between healthcare providers' attitudes and the content of information (r = .622; p < .0001), and content of information and parents' responses (r = .271; p = .002).
By providing positive information, healthcare providers can help parents to develop a more optimistic outlook on their children's future.
Journal for Specialists in Pediatric Nursing 04/2011; 16(2):113-20. · 0.78 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A 77-year-old woman underwent chemotherapy, radiotherapy, and brachytherapy for cervical cancer 9 years ago. On a follow-up
18F-fluorodeoxyglucose (FDG) PET/CT image, focal FDG uptake was noted in a focal osteolytic lesion in the right femoral head.
During magnetic resonance imaging, this lesion showed subchondral dark-signal-intensity rim on T1-weighted image and double
line sign on T2-weighted image, suggestive of osteonecrosis. The lesion was pathologically confirmed as osteonecrosis after
surgery. This case demonstrates that osteonecrosis of the femoral head may demonstrate focal FDG uptake mimicking bone metastasis.
KeywordsAvascular necrosis–Femoral head–Bone metastasis–
18F-fluorodeoxyglucose–Positron emission tomography/computed tomography