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Publications (3)5.52 Total impact

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    ABSTRACT: Nous avons étudié la gamme des hôtes d'un mélange de virus de la nucléopolyhédrose (CfMNPV et CfDefNPV) de Choristoneura fumiferana (Clemens) au moyen de bioessais par voie orale chez des larves de 29 espèces de lépidoptères et des mâles adultes de Megachile rotundata (F.) (Hymenoptera : Megachilidae). À l'aide d'une sonde ADN de génome entier, nous avons obtenu des résultats positifs chez 8 de 10 tortricidés, Archips cerasivorana (Fitch), Choristoneura fractivittana (Clemens), C. fumiferana, Choristoneura occidentalis Freeman, Choristoneura pinus pinus Freeman, Choristoneura rosaceana (Harris), Clepsis persicana (Fitch) et Cydia pomonella (L.) ; un crambidé, Ostrinia nubilalis (Hübner) ; un érebidé arctiiné, Estigmene aerea (Drury); et deux noctuidés, Oligia illocata (Walker) et Pyrrhia exprimens (Walker). Les taux de mortalité sont maximaux chez C. fumiferana, C. occidentalis, C. pinus pinus, A. cerasivorana et C. pomonella. Les amplicons séquences de l'amplification en chaîne par polymérase (PCR) provenant d'individus infectés appartenant à plusieurs espèces confirment que les jeux d'amorces amplifient les virus ciblés. Le CfMNPV se retrouve constamment chez les C. fumifera nourris de virus, alors que le CfDefNPV n'apparaît qu'occasionnellement. La présence du CfMNPV et du CfDefNPV chez A. cerasivorana est confirmée par la PCR et le séquençage d'ADN. Des taux de mortalité significatifs reliés au traitement ont été provoqués chez les noctuidés P. exprimens et Acronicta impleta Walker; la PCR a déterminé que les deux virus sont présents chez les P. exprimens traités, mais seul CfMNPV apparaît chez A. impleta. Aucun virus n'a été décelé chez M. rotundata. [Traduit par la Rédaction]
    The Canadian Entomologist 04/2011; · 0.90 Impact Factor
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    ABSTRACT: To assess the persistence of genetically modified and naturally occurring baculoviruses in an aquatic environment, replicate (three) outdoor, aquatic microcosms were spiked with spruce budworm viruses [Ireland strain of Choristoneura fumiferana multiple nucleopolyhedrovirus (CfMNPV) and the recombinant CfMNPVegt(-)/lacZ(+)] at a rate of 1.86 x 10(10) occlusion bodies (OBs) m(-2) of surface area. The presence of virus in water samples collected at various times after inoculation was determined by PCR amplification of baculoviral DNA extracted from OBs. Although UV radiation rapidly degrades baculoviruses under natural conditions, both viruses persisted above the level of detection (>100 OBs 450 microL(-1) of natural pond water) for at least 1 year post-inoculation, with little difference between the viruses in their patterns of persistence. The present microcosm study suggests that occlusion bodies of baculoviruses can persist in the flocculent layer of natural ponds. On disturbance, OBs could re-enter the main water column and thus be available for transport to new locations. Implications for environmental risk assessment are discussed.
    Pest Management Science 06/2008; 64(10):1015-23. · 2.74 Impact Factor
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    ABSTRACT: Genetically-modified baculoviruses have potential uses as bio-pesticides in forestry. However, the baculoviral occlusion bodies (OBs) may release genetically-modified DNA into the forest environment. In this research, outdoor aquatic microcosms, spiked with 673 microg of genomic DNA (4.4 x 10(12) target copies) from the genetically modified baculovirus Choristoneura fumiferana MNPVegt-/lacZ+, were exposed to natural summer conditions. A 530 bp DNA fragment from the genome of CfMNPVegt-/lacZ+ was detected in field microcosm water samples for about 24 h. The introduced DNA may have persisted for a longer time, but was below the detection limit of the PCR analysis (13.5 pg DNA or 8.9 x 10(4) target copies ml(-1) water). The detection limit of PCR was determined by spiking water samples with a dilution series of CfMNPVegt-/lacZ+ genomic DNA, extracting and purifying the DNA, and then PCR analysis. This study provides some of the first information on the persistence and detection limits of this viral DNA under aquatic ecological conditions, and the methods that can be used to conduct such a molecular-based field study.
    Molecular and Cellular Probes 05/2005; 19(2):75-80. · 1.87 Impact Factor