Publications (3)1.81 Total impact
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Article: [Detection of serum biomakers of osteosarcoma by proteomic profiling].
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ABSTRACT: To detect differentially expressed proteins in serum of patient with osteosarcoma. 8 serum protein samples were recruited (4 cases of osteosarcoma and 4 cases of normal adults), cross-labeled with variant CyDye, followed by two-dimensional differential in-gel electrophoresis (2-D DIGE), image analysis, and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). 24 protein spot-features were significantly increased, and 34 were significantly decreased in the serum from patients with osteosarcoma relative to the controls. The mass spectrometry analysis revealed 18 unique proteins that were increased, and 25 unique proteins decreased in the serum of patients with osteosarcoma. Gelsolin was down-regulated in osteosarcoma, and Western blotting also confirmed a decreased level of gelsolin in the serum of patients with osteosarcoma. Our results indicate that gelsolin may have great potential as a biomarker of osteosarcoma and as a potential target for therapy. These preliminary data suggest that incorporation of more samples and new datasets will permit the identification of serum biomarkers for osteosarcoma.Zhonghua zhong liu za zhi [Chinese journal of oncology] 08/2008; 30(7):519-22. -
Article: [Serum proteomic variation study in patients with Crohn disease].
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ABSTRACT: To search differentially expressed proteins in serum of patients with Crohn disease. Serum protein samples from 4 patients with Crohn disease and 8 healthy adults were recruited cross-labeled with variant CyDye, and then followed by two-dimensional differential in-gel electrophoresis (2-D DIGE), image analysis, and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The 2-D electrophoresis results were compared between the Crohn disease patients and the healthy adults. The spot 1058 expression in serum of Crohn disease patients increased by 1.68 folds as compared with healthy adults (P<0.05). The protein was identified as haptoglobin by mass spectrometry. Up-regulating expression of haptoglobin in serum of Crohn disease patients may play a role in disequilibrium of immunity system.Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery 05/2008; 11(3):266-9. -
Article: 2‐D DIGE and MALDI‐TOF‐MS analysis of the serum proteome in human osteosarcoma
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ABSTRACT: We performed 2-D DIGE on proteins prepared from serum obtained from patients with osteosarcoma (OS) and controls, to identify differentially expressed proteins that might serve as serum biomarkers for OS prognosis. Proteins found to be differentially expressed were identified by MALDI-TOF mass spectrometric analysis, coupled with database interrogation. We compared serum samples from four individuals with OS to four age- and sex-matched healthy controls. We identified 24 protein spot-features that were significantly increased, and 34 that were significantly decreased in serum from patients with OS relative to the controls. The MS analysis revealed 18 unique proteins that were increased, and 25 unique proteins that were decreased in OS serum samples. Western blot and ELISA analysis confirmed increased levels of amyloid-related serum protein (SAA) in the OS serum samples. The increased expression levels of SAA were decreased after using MTX and cisplatin combination chemotherapy, and were further decreased after operation. Moreover, increased expression levels of sera SAA were seen in the relapsed patients. Our results suggested that the determination of serum SAA in OS patients might be utilized as a marker for relapse and in evaluation of the efficacy of therapy.PROTEOMICS - CLINICAL APPLICATIONS 03/2007; 1(3):272 - 285. · 1.81 Impact Factor
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2007
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Sun Yat-Sen University
- Proteomics Lab
Guangzhou, Guangdong Sheng, China
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