Shaoping Hu

Publications (3)10.03 Total impact

• Article: Transformation of Lead Solid Fraction in the Rhizosphere of Elsholtzia splendens: The Importance of Organic Matter

  Jianjun Yang, Shaoping Hu, Xincai Chen, Mingge Yu, Jin Liu, Hang Li, Chaofeng Shen, Jiyan Shi, Yingxu Chen
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  ABSTRACT: The rhizosphere, enriched in organic matter, is the bottleneck of metal transfer in the soil–plant system. However, the transformation of metal fractions in the rhizosphere and the mechanisms that are involved, notably the role of organic matter, are poorly known. In this study, the solid-phase fractionation of lead (Pb) in the rhizosphere and non-rhizosphere soil of Elsholtzia splendens in a Pb-contaminated soil was investigated using a nine-step selective sequential extraction method in a pot experiment. Compared to the non-rhizosphere soil, there were measurable increases in Pb-fulvic complexes, Pb-humic complexes, organic Pb, and amorphous Pb but no significant changes in other forms of Pb in the rhizosphere soil. Pb-fulvic complexes and organic Pb, increasing from 397 to 438mg kg−1 and 229 to 258mg kg−1, respectively, showed a stronger accumulating trend than Pb-humic complexes and amorphous Pb, with an increase from 15.9 to 17.3mg kg−1 and 6.04 to 7.80mg kg−1 respectively, in the rhizosphere soil relative to non rhizosphere soil. These results may be mainly due to the enrichment of organic matter in the rhizosphere soil, resulting from root exudation and the enhanced turnover of microorganisms. The accumulation of Pb-fulvic complexes in the rhizosphere soil increases the potential phytoavailable pool, thus likely facilitating the phytoextraction of Pb in metal-contaminated soil.
  Water Air and Soil Pollution 04/2012; 205(1):333-342. · 1.63 Impact Factor
• Article: Proteomic characterization of copper stress response in Elsholtzia splendens roots and leaves.

  Feng Li, Jiyan Shi, Chaofeng Shen, Guangcun Chen, Shaoping Hu, Yingxu Chen
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  ABSTRACT: Elsholtzia splendens is generally considered as a Cu-tolerant and -accumulating plant species, and a candidate for phytoremediation of Cu-contaminated soils. To better understand the Cu tolerance/accumulation mechanisms in E. splendens, proteomic analysis was performed on E. splendens roots and leaves exposed to 100 muM CuSO(4) for 3 and 6 days. After 6 days of treatment, Cu accumulation in roots increased much more than that in leaves. SDS-PAGE analysis showed that the proteins changed more intensively in roots than did in leaves upon Cu stress. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 45 protein spots were significantly changed in roots, but only six protein spots in leaves. The abundance of protein spots mostly showed temporal changes. MALDI-TOF MS and LTQ-ESI-MS/MS were used to identify the differently expressed protein spots. The identified root proteins were involved in various cellular processes such as signal transduction, regulation of transcription and translation, energy metabolism, regulation of redox homeostasis and cell defense. The leaf proteins were mainly degraded fragments of RuBisCo and antioxidative protein. The roles of these proteins in Cu tolerance/accumulation were discussed. The resulting differences in protein expression pattern suggested that redirection of root cellular metabolism and redox homeostasis might be important survival mechanisms of E. splendens upon Cu stress.
  Plant Molecular Biology 08/2009; 71(3):251-63. · 4.15 Impact Factor
• Source

  Available from: zju.edu.cn

  Article: Interaction of Pseudomonas putida CZ1 with clays and ability of the composite to immobilize copper and zinc from solution.

  XinCai Chen, ShaoPing Hu, ChaoFeng Shen, ChangMing Dou, JiYan Shi, YingXu Chen
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  ABSTRACT: The present study was conducted to determine the abilities of the living and nonliving Pseudomonas putida CZ1 cells, clays (goethite, kaolinite, smectite and manganite) and their composites to accumulate copper and zinc from a liquid medium, and elucidate the role of microbes on the mobility of heavy metals. Various mixtures of bacteria and clays were exposed to solutions of 0.025 mM or 0.5mM Cu(II) and Zn(II) in 0.01M KNO(3) to differentiate between so-called "high-affinity" sites and "low-affinity" sites. Clays associated in an edge-on orientation to the cells was observed by electron microscope (EM) examination of these metal-treated bacteria-clay aggregates. Adsorption experiments and desorption with 1.0M CH(3)COOK solution indicated that clays contain more high-affinity copper binding sites and less high-affinity zinc binding sites than that of bacteria, however, bacteria are involved in more low-affinity heavy-metal-binding sites. Carboxyl group activity is more important at weak-binding sites than at strong-binding sites. TEM-EDS analysis confirmed that most of Zn removed from solution was associated with P. putida CZ1 in the composites. These results suggest that bacteria play an important role in regulating the mobility of heavy metals in the soil environment.
  Bioresource technology 06/2008; 100(1):330-7. · 4.25 Impact Factor