Lin Yang

Minzu University of China, 民主, Fujian, China

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Publications (17)18.56 Total impact

  • Tetrahedron. 01/2014; 70(22):3560–3569.
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    ABSTRACT: The major phenylpropanoids in the cell culture, regenerated plantlets and herbs of Saussurea involucrata were systematically and comparatively investigated. A total of 17 constituents were identified on the basis of HPLC-DAD/ESI-MS(n) and HPLC-ESI-IT-TOF/MS analyses. Among them, 13 constituents were unambiguously identified by comparing the retention time, UV, MS and MS(n) spectra of samples with standards/literature, and the other 4 constituents were tentatively assigned on the basis of their UV spectra and MS(n) fragmentation patterns. In addition, a quantification method for the simultaneous quantification of 3 major phenylpropanoids syringin, 5-caffeoylquinic acid, and 1,5-dicaffeoylquinic acid was successfully established. The established HPLC quantification method was proved to have excellent linearity, precision, repeatability and accuracy. These studies provide a secondary metabolic profile of the cell cultures of S. involucrata, which is valuable for improving the quality control of cell culture and sheds light on the biosynthetic pathway of phenylpropanoids in this species.
    Journal of pharmaceutical and biomedical analysis 02/2013; 74:39-46. · 2.45 Impact Factor
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    ABSTRACT: Ten new abietane diterpenoids, aquilarabietic acids A-J (1-10), and a new podocarpane diterpenoid, aquilarabietic acid K (11), were isolated from the petroleum ether and ethanol extracts of Chinese eaglewood. Among them, 3, 9, and 10 are artifacts. Their structures were established on the basis of data from extensive spectroscopic and X-ray diffraction analyses. Bioassay results indicated that 1 at 10 μM demonstrated remarkable antidepressant activity in vitro by inhibiting norepinephrine reuptake in rat brain synaptosomes by 81.4% and with an IC(50) value of 9.1 × 10(-7) M.
    Journal of Natural Products 02/2013; · 3.29 Impact Factor
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    ABSTRACT: The column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds from the EtOAc extract of medium and MeOH extract of cell cultures of Morus alba. Eight compounds were isolated. Based on physico-chemical properties and spectroscopic data, their structures were identified as isobavachalcone (1), genistein (2), norartocarpetin (3), albanin A (4), guangsangon E (5), mulberrofuran F (6), chalcomoracin (7), kuwanon J (8). Compounds 3-6 were isolated from the cell cultures of M. alba for the first time.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 12/2012; 37(24):3738-42.
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    ABSTRACT: Ten compounds were isolated and purified from cell suspension cultures of Cudrania tricuspidata with silica-gel column chromatography, semi-preparative HPLC and Sephadex LH-20. On the base of their physicochemical properties and spectral data, their structures were identified as 1, 3, 5-trihydroxy4-(3, 3-dimethylallyl) xanthone (1), wighteone (2), 6-prenylapigenin (3), licoflavone C(4), cudraflavanone C(5), erythrivarone A (6), derrone (7), carthamidin (8), genistein (9) and aromadendrin (10). Among them, compounds 2-10 were flavonoids, and compound 1 was a xanthone which was isolated from the plant for the first time.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 12/2012; 37(24):3734-7.
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    ABSTRACT: Two new sesquiterpene derivatives, (+)-8β-hydroxy-longicamphenylone (1) and 11β-hydroxy-13-isopropyl-dihydrodehydrocostus lactone (2), were isolated from Chinese eaglewood. Their structures were elucidated on the basis of spectroscopic analyses including HR-ESI-MS, (1)H and (13)C NMR, DEPT, (1)H-(1)H COSY, HSQC, HMBC, and NOE. The two compounds exhibited potent anti-depressant activity in vitro by inhibiting [(3)H]-5-HT reuptake in rat brain synaptosomes by 54.8% and 51.9%, respectively, at 10 μM.
    Journal of Asian natural products research 08/2012; · 0.61 Impact Factor
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    ABSTRACT: The column chromatography on silica gel, semi-preparative HPLC were used to separate and purify the compounds from the petroleum ether and ethanol extract of Aquilaria sinensis. Nine compounds were isolated. On the basis of their spectroscopic data, the structures were identified as 3, 3, 7-trimethyltricycloundecan-8-one (1), longifolene (2), norlongilactone (3), caryophyllenol-II (4), humulene diepoxide A (5), kobusone (6), (-)-bornyl ferulate (7), (24R) -24-ethylcholesta-4, 22-dien-3-one (8), (24R)-24-3-ono-4-en-sitosterone (9). Compounds 2-9 were isolated from this plant for the first time. compounds 1-6 are sesquiterpenes, compound 7 is a monoterpene derivative, compound 8 and 9 are steroids.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 07/2012; 37(13):1973-6.
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    ABSTRACT: 2-(2-Phenylethyl) chromones and a 2-(2-phenylethenyl) chromone, were isolated from the ethanolic extract of Chinese eaglewood. Their structures were determined on the basis of extensive analyses of spectroscopic data. Among those, one showed significant neuroprotective activities against both glutamate-induced and corticosterone-induced neurotoxicity in P12 pheochromocytoma and human U251 glioma cells at a concentration of 10 μM and increased cell viability by 82.2% and 86.9%, respectively.
    Phytochemistry 04/2012; 76:92-7. · 3.05 Impact Factor
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    ABSTRACT: Two new neolignans (1, 2) and six sesquiterpenes (3- 8) were isolated from the cell cultures of Stellera chamaejasme. Their structures and absolute configurations were elucidated by extensive spectroscopic and computational methods. Compound 4 exhibited significant protective effects against CCl (4)-induced hepatotoxicity in HepG2 cells, reducing aspartate aminotransferase release by 29.49 % at 10 µM. These compounds have not been isolated from plant material, which implies that in vitro plant cell cultures may offer alternative and effective sources of bioactive natural compounds.
    Planta Medica 03/2012; 78(7):711-9. · 2.35 Impact Factor
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    ABSTRACT: From callus cultures of Stellera chamaejasme, 17 compounds were isolated. Based on their physical and chemical data and spectroscopic analysis, they were identified as syringaresinol (1), medioresinol (2), pinoresinol (3), (1R, 2S, 5R, 6S)- 2-(4- hydroxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3, 7-dioxabicyclo [3, 3, 0] octane (4), epipinoresinol (5), caruilignan D (6), 3-oxo-guai-4-ene-11, 12-diol (7), (-) -lariciresinol (8), tetrahydro-2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxyphenyl) methyl]-3-furanmethanol (9), 5'-methoxylariciresinol (10), vladinol D (11), cyclo (L-Pro-L-Val) (12), oxomatairesinol (13), (+) -guayarol (14); acutissimalignan B (15), isolariciresinol (16), and beta-sitosterol (17), respectively. Among these compounds, 12 was a cyclodipeptide, 7 was a sesquiterpene, and the others except 17 were lignans. All compounds were first isolated from callus cultures of S. chamaejasme.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 12/2011; 36(24):3457-62.
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    ABSTRACT: Column chromatography on silica gel, Sephadex LH-20, semi-preparative HPLC were used to separate and purify the compounds from the petroleum ether and ethanol extract of Chinese eaglewood. Nine compounds were isolated. On the basis of their spectroscopic data, their structures were identified as dehydroabietic acid (1), methyl dehydroabietate (2), methyl 7-oxodehydroabietate (3), 7alpha, 15-dihydroxydehydroabietic acid (4), 7alpha-hydroxypodocarpen-8(14)-en-13-on-18-oic acid (5), pimaric acid (6), pimarol (7), 18-norpimara-8 (14), and 15-dien-4alpha-ol (8), 18-norisopimara-8 (14), 15-dien-4beta-ol (9). All of the compounds were isolated from this plant for the first time, and compounds 5, 8 and 9 are norditerpenoids.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 08/2011; 36(15):2088-91.
  • Lin Yang, Jungui Dai
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    ABSTRACT: The fungus, Absidia coerulea was employed to bioconvert tetrahydro-alpha-santonins, 1,2,4alpha,5alpha-tetrahydro-alpha-santonin (1), and its 4-epimer (2), from which 10 products (3-12) were obtained. Furthermore, their structures were determined, based on their chemical and spectroscopic data analyses. Among them, 3-5, 7, 9, 11 and 12 were observed to be seven new compounds. The reactions mainly involved in these bio-process included hydroxylation(s) (C-4, C-11, and C-1), reduction (C-3 ketone to alcohol).
    Natural Product Research 05/2008; 22(6):499-506. · 1.03 Impact Factor
  • Journal of Molecular Catalysis B-enzymatic - J MOL CATAL B-ENZYM. 01/2007; 46(1):8-13.
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    ABSTRACT: Cell suspension cultures of five plants (Catharanthus roseus, Ginkgo biloba, Platycodon grandiflorum, Taxus cuspidata, Phytolacca asinosa) were employed to bioconvert the eudesmanolide compound, alpha-santonin. Reactions occurring were hydroxylation (C-1, C-11 and C-15), reduction of the double bond [1(2) or 3(4)], rearrangment of the eudesmanolide skeleton to a guaianolide skeleton and lactone-ring hydrolysis. Four new compounds were identified.
    Biotechnology Letters 07/2005; 27(11):793-7. · 1.85 Impact Factor
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    ABSTRACT: Taxuyunnanine C (1) and its analogs (2 and 3), the C-14 oxygenated 4(20), 11-taxadienes from callus cultures of Taxus sp., were regio- and stereo-selectively hydroxylated at the 7β position by a fungus, Abisidia coerulea IFO 4011, and it was interesting that the longer the alkyl chain of the acyloxyl group at C-14 became, the higher the yield of 7β-hydroxylated product was. Besides the three 7β-hydroxylated products (5, 9, 17), other nine new products (7, 11, 12, 14, 15, 16, 18, 20 and 21) and six known products (4, 6, 8, 10, 13 and 19) were obtained. Subsequently, the acetylated derivatives (24 and 27) of 7β-and 9α-hydroxylated products of 1 were regio- and stereo-specifically hydroxylated at the 9α position by Ginkgo cells and 7β position by A. coerulea, respectively. Thus, the two specific oxidations have been combined. These bioconversions would provide not only valuable intermediates for the semi-synthesis of paclitaxel or other bioactive taxoids from 1 and its analogs, but also some useful hints for the biosynthetic pathway of taxoid in the natural Taxus plant.
    Tetrahedron. 01/2005; 61(23):5507-5517.
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    ABSTRACT: Agilawood is a rare and valuable traditional medicine used worldwide since ancient times. However, its formation in the tree Aquilaria sinensis under natural conditions takes a long time. Fungi with high levels of bioactivity for triggering agilawood formation could enable the establishment of plantations of A. sinensis for improved production of this traditional medicine in Hainan Province, China. The objective of the present study was to evaluate the chemical components of agilawood induced in A. sinensis by inoculation with the fungus Paraconiothyrium variabile. The results using by GC-MS and HPLC indicated that sesquiterpenoids, chromones and their analogues were produced when the fungus was inoculated for 8 months into the host tree. Dibutyl phthalate and Phenol,2,2′-methylenebis[6-(1,1-dimethylethyl)- 4-methyl]-, the main components of A. sinensis, wood were converted into components of agilawood. The data showed that the fungus-induced agilawood samples were similar to natural agilawood and could be used as an agilawood substitute. This is the first time that agilawood formation has been shown to result from inoculation of A. sinensis with the bioactive fungus P. variabile.
    Symbiosis 60(1). · 1.11 Impact Factor
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    ABSTRACT: The cell suspension cultures of Cudrania tricuspidata could regio-selectively prenylate chrysin (1) at C-8, while the cell suspension cultures of Morus alba could regio-selectively prenylate genistein (2), sophoricoside (3) and diosmetin (4) at C-6. Eight products (5–12) were isolated, and five of them (8–12) were new compounds. Additionally, the bioconversion of 1–4 using microsomes of the cell cultures was performed, and the results showed that the bioconversion patterns were identical to those using cell cultures. These investigations would provide an approach to the selective prenylation and structural diversification of flavonoids.
    Journal of Molecular Catalysis B Enzymatic 89:28–34. · 2.82 Impact Factor

Publication Stats

18 Citations
18.56 Total Impact Points

Institutions

  • 2012–2014
    • Minzu University of China
      民主, Fujian, China
  • 2005–2008
    • South-Central University For Nationalities
      Peping, Beijing, China