Wen Tang

Nanjing University of Technology, Nanjing, Jiangsu Sheng, China

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Publications (2)0 Total impact

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    ABSTRACT: We used genetic methods to get a mutational spt15 gene from the recombinant strain Saccharomyces cerevisiae YPH499-3, screened by global transcription machinery engineering (gTME) approach. We transformed the gene into the original strain Saccharomyces cerevisiae YPH499 using the vector pYX212, then got a new recombinant strain. We studied the characteristic of this strain and found that it could metabolize xylose and co-ferment xylose and glucose. Under the fermentation condition of 30 degrees C, 200 r/min, 72 h, the utilization ratio of xylose was 82.0%, with 32.4% of ethanol yield when the carbon source in the media was 50 g/L xylose, while the utilization ratio of xylose and glucose was 80.4% and 100% respectively, with the 31.4% of ethanol yield when the carbon source was 50 g/L glucose/xylose (1:1). Meanwhile, the concentration of the by-product xylitol was very low. This study demonstrates the effect which the forward mutation of spt15 gene makes to the co-fermentation of xylose and glucose to ethanol by Saccharomyces cerevisiae.
    Sheng wu gong cheng xue bao = Chinese journal of biotechnology 06/2009; 25(6):875-9.
  • Wen Tang, Ming Yan
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    ABSTRACT: We studied the secretomics' properties of Trichoderma reesei, an important industrial microorganism used for cellulase production. We analyzed the amino acid sequences coded by 9997 ORFs in Trichoderma reesei genome with bioinformatics approaches, identified 294 possible secreted protein sequences, and classified them by functions. We also applied motif search methods to search key motifs in the function-unknown sequences and preliminary predicted their functions. Moreover, we analyzed the signal peptide sequences of the secreted proteins. There were 188 hydrolytic enzymes in Trichoderma reesei's secretomics, including 114 glycosidases, 42 proteases, and 11 lipoidases. The glycosidases included 22 reported cellulases, and 15 chitinases, as well as 30 other protein sequences probably related to cellulose degradation. The homology of signal peptides of secreted proteins was low whereas sequences near the digesting site of signalase were conservative. This method gave insights into the whole secreted proteome of Trichoderma reesei and provided basis for further studies on secretomic features at a genome level.
    ACTA MICROBIOLOGICA SINICA 05/2008; 48(4):473-9.