Daniel Seifert

Publications (2)1.17 Total impact

• Article: Radioiodination of mouse anti-III beta-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies.

  Helena Svecová, Veronika Kleinová, Daniel Seifert, Hana Chaloupková, Jörg Bäurle, Karel Kranda, Vlastimil Král, Miroslav Fiser
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  ABSTRACT: The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125 I in order to develop new imaging agents against the specific neuronal marker III beta-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
  Applied Radiation and Isotopes 04/2008; 66(3):310-6. · 1.17 Impact Factor
• Article: Radioiodination of mouse anti-III β-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies

  Helena Švecová, Veronika Kleinová, Daniel Seifert, Hana Chaloupková, Jörg Bäurle, Karel Kranda, Vlastimil Král, Miroslav Fišer
  [show abstract] [hide abstract]
  ABSTRACT: The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125I in order to develop new imaging agents against the specific neuronal marker III β-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
  Applied Radiation and Isotopes.