Jong-Hyun Lee

Kyung Hee University, Seoul, Seoul, South Korea

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Publications (3)5.84 Total impact

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    ABSTRACT: Abstract Background and objectives: Saengmaeksan (SMS) is a Korean herbal prescription consisting of three different herbal drugs: Liriopis Tuber (tuber of Liriope platyphylla, Liliaceae), Ginseng Radix (root of Panax ginseng) and Schisandrae Fructus (fruit of Schisandra chinensis). SMS is commonly used in Korea to treat various diseases that involve the respiratory and cardiovascular systems. However, to date, the mechanism underlying the anti-inflammatory effects of SMS is not clearly understood. In this study, we attempt to determine the effects of SMS on lipopolysaccharide (LPS)-induced inflammatory responses in mouse peritoneal macrophages. Methods: Cell viability was measured by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and nitric oxide (NO) levels were measured by using Griess reagent. The tumor necrosis factor (TNF)-α and interleukin (IL)-6 levels secreted by the cells were measured using a modified enzyme-linked immunosorbent assay. Expression of cyclooxygenase (COX)-2 and nuclear factor-kappa B (NF-κB), respectively was investigated using a western blot analysis. A caspase colorimetric assay kit was used to assay enzymatic caspase-1 activity. Results: The findings of this study showed that SMS reduced TNF-α and IL-6 production induced by LPS. During the inflammatory process, COX-2 and NO levels were increased in mouse peritoneal macrophages, but SMS decreased the enhanced levels of COX-2 and the production of NO. In addition, SMS suppressed the activation of NF-κB and receptor interacting protein-2/caspase-1. Discussion and conclusion: Our results provide novel insights into the pharmacological actions of SMS, a molecule that can potentially be exploited in the treatment of inflammatory diseases.
    Immunopharmacology and Immunotoxicology 01/2013; · 1.36 Impact Factor
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    ABSTRACT: Using mouse peritoneal macrophages, we have examined the mechanism by which Leonurus sibiricus (LS) regulates nitric oxide (NO) production. When LS was used in combination with recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production; however, LS by itself had no effect on NO production. The increased production of NO from rIFN-gamma plus LS-stimulated cells was almost completely inhibited by pretreatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappaB. Furthermore, treatment of peritoneal macrophages with rIFN-gamma plus LS caused a significant increase in tumor necrosis factor-alpha (TNF-alpha) production. PDTC also decreased the effect of LS on TNF-alpha production significantly. Because NO and TNF-alpha play an important role in immune function and host defense, LS treatment could modulate several aspects of host defense mechanisms as a result of stimulation of the inducible nitric oxide synthase.
    Canadian Journal of Physiology and Pharmacology 11/2008; 86(10):682-90. · 1.55 Impact Factor
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    ABSTRACT: Nicotine is a major pharmacologically active component of cigarette smoke. Excessive cigarette smoking is harmful to lung. Sejin-Eum (SJE) I is composed of various Oriental medicines, and SJE II is SJE I plus seeds of Avena sativa (Gramineae) that reduces the craving for cigarette in man. In this study, we have examined whether an aqueous extract of SJE I/II inhibits nicotine- or cigarette extract (CE)-induced cytotoxicity in human embryonic lung fibroblast, MRC-9. Assessment of cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay indicated that SJE I/II (500 and 1000 microg/ml) not only inhibited nicotine-induced cytotoxicity but also had significantly proliferous effect on MRC-9. However, SJE I/II had little effect on inhibition of CE-induced cytotoxicity. These results suggest the possibility that the use of SJE I/II may be useful for improvement of many symptoms by nicotine.
    Journal of Ethnopharmacology 06/2003; 86(1):15-20. · 2.94 Impact Factor