Fang Liu

Publications (2)0 Total impact

• Article: [Effect of separation and purification processes on extraction ratio of polysaccharides in Rabdosia rubescens and immunocompetence].

  Fang Liu, Pei-Yi Liu, Lan-Fang Hao, Jing Zhou
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  ABSTRACT: To select a good method for purifying polysaccharides from Rabdosia rubescens, and study their immunological activity. DEAE-Sepharose Fast Flow (Cl-) weak polar anion-exchange chromatography and stepwise ethanol precipitation were used to isolate and purify the polysaccharides from Rabdosia rubescens, respectively. MTT method was used to evaluate the immune activity. Five components were obtained from DEAE-Sepharose Fast Flow (Cl-) weak polar anion-exchange chromatography and four polysaccharides were obtained through alcohol precipitation. Polysaccharides from Rabdosia rubescens by stepwise ethanol precipitation show stronger immunological activity. Their immunomodulatory effect and related mechanism will be researched further.
  Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 03/2011; 34(3):449-52.
• Article: [Study on deproteinization and decoloration in extraction of polysaccharides from Rabdosia rubescens].

  Fang Liu, Xue-Hui Zhu, Mei Liu, Jing Zhou, Xian-Ping Che, Rui-Fa Han
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  ABSTRACT: To screen the method on deproteinization and decoloration in extraction of of polysaccharides from Rabdosia rubescens. The effect of deproteinization with different methods was evaluated in terms of the ratio of protein removing and polysaccharide removing, the decoloration effect was also calculated. The deproteinization effect was optimal with TCA method and activated carbon also achieved a high decoloration rate. The result can establish foundation for the further study of the polysaccharides from Rabdosia rubescens.
  Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 06/2008; 31(5):751-3.