H L Li

Yunnan Agricultural University, Panlong, Shaanxi, China

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Publications (10)21.89 Total impact

  • D H Zhou · Z G Yuan · F R Zhao · H L Li · Y Zhou · R Q Lin · F C Zou · H Q Song · M J Xu · X Q Zhu
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    ABSTRACT: Toxoplasma gondii is an obligate intracellular protozoan parasite, which can invade and multiply within the macrophages of humans and most warm-blooded animals. Macrophages are important effector cells for the control and killing of intracellular T. gondii, and they may also serve as long-term host cells for the replication and survival of the parasite. In the present study, we explored the proteomic profile of macrophages of the specific pathogen-free Kunming mice at 24 h after infection with tachyzoites of the virulent T. gondii RH strain using two-dimensional gel electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight (TOF)/TOF tandem mass spectrometry. Totally, 60 differentially expressed protein spots were identified. Among them, 52 spots corresponded to 38 proteins matching to proteins of the mouse, including actin, enolase, calumenin, vimentin, plastin 2, annexin A1, cathepsin S, arginase-1, arachidonate 12-lipoxygenase, and aminoacylase-1. Functional prediction using Gene Ontology database showed that these proteins were mainly involved in metabolism, structure, protein fate, and immune responses. The findings provided an insight into the interactive relationship between T. gondii and the host macrophages, and will shed new lights on the understanding of molecular mechanisms of T. gondii pathogenesis.
    Parasitology Research 05/2011; 109(6):1637-46. DOI:10.1007/s00436-011-2435-z · 2.33 Impact Factor
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    ABSTRACT: The present study examined sequence variability in a portion of the mitochondrial cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase subunits 4 and 5 (pnad4 and pnad5) among 39 isolates of Fasciola spp., from different hosts from China, Niger, France, the United States of America, and Spain; and their phylogenetic relationships were re-constructed. Intra-species sequence variations were 0.0-1.1% for pcox1, 0.0-2.7% for pnad4, and 0.0-3.3% for pnad5 for Fasciola hepatica; 0.0-1.8% for pcox1, 0.0-2.5% for pnad4, and 0.0-4.2% for pnad5 for Fasciola gigantica, and 0.0-0.9% for pcox1, 0.0-0.2% for pnad4, and 0.0-1.1% for pnad5 for the intermediate Fasciola form. Whereas, nucleotide differences were 2.1-2.7% for pcox1, 3.1-3.3% for pnad4, and 4.2-4.8% for pnad5 between F. hepatica and F. gigantica; were 1.3-1.5% for pcox1, 2.1-2.9% for pnad4, 3.1-3.4% for pnad5 between F. hepatica and the intermediate form; and were 0.9-1.1% for pcox1, 1.4-1.8% for pnad4, 2.2-2.4% for pnad5 between F. gigantica and the intermediate form. Phylogenetic analysis based on the combined sequences of pcox1, pnad4 and pnad5 revealed distinct groupings of isolates of F. hepatica, F. gigantica, or the intermediate Fasciola form irrespective of their origin, demonstrating the usefulness of the mtDNA sequences for the delineation of Fasciola species, and reinforcing the genetic evidence for the existence of the intermediate Fasciola form.
    Veterinary Parasitology 04/2011; 181(2-4):329-34. DOI:10.1016/j.vetpar.2011.03.057 · 2.55 Impact Factor
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    ABSTRACT: The present study examined sequence variability in a portion of the mitochondrial cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase subunits 4 and 5 (pnad4 and pnad5) among 39 isolates of Fasciola spp., from different hosts from China, Niger, France, the United States of America, and Spain; and their phylogenetic relationships were re-constructed. Intra-species sequence variations were 0.0-1.1% for pcox1, 0.0-2.7% for pnad4, and 0.0-3.3% for pnad5 for Fasciola hepatica; 0.0-1.8% for pcox1, 0.0-2.5% for pnad4, and 0.0-4.2% for pnad5 for Fasciola gigantica, and 0.0-0.9% for pcox1, 0.0-0.2% for pnad4, and 0.0-1.1% for pnad5 for the intermediate Fasciola form. Whereas, nucleotide differences were 2.1-2.7% for pcox1, 3.1-3.3% for pnad4, and 4.2-4.8% for pnad5 between F. hepatica and F. gigantica; were 1.3-1.5% for pcox1, 2.1-2.9% for pnad4, 3.1-3.4% for pnad5 between F. hepatica and the intermediate form; and were 0.9-1.1% for pcox1, 1.4-1.8% for pnad4, 2.2-2.4% for pnad5 between F. gigantica and the intermediate form. Phylogenetic analysis based on the combined sequences of pcox1, pnad4 and pnad5 revealed distinct groupings of isolates of F. hepatica, F. gigantica, or the intermediate Fasciola form irrespective of their origin, demonstrating the usefulness of the mtDNA sequences for the delineation of Fasciola species, and reinforcing the genetic evidence for the existence of the intermediate Fasciola form.
    Veterinary Parasitology 04/2011; 37(1):107-120. · 2.55 Impact Factor
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    C Yan · C L Yue · S B Qiu · H L Li · H Zhang · H Q Song · S.Y. Huang · F C Zou · M Liao · X Q Zhu
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    ABSTRACT: Little information on epidemiology of Toxoplasma gondii infection in pigeons was available in People's Republic of China. In the present study, sera from 275 pigeons raised in different commercial flocks in Guangdong Province of southern China were evaluated using modified agglutination test (MAT). Specific antibodies were found in sera of 8.7% of 275 pigeons (MAT titer ≥ 1:5), and the seropositivity of eight herds we surveyed varied ranging from 0 to 18.2%. The results demonstrated the circulation of T. gondii in the examined pigeon farms, which poses potential risk for human infection with T. gondii. To our knowledge, this is the first seroprevalence survey of pigeons infected by T. gondii in People's Republic of China.
    Veterinary Parasitology 12/2010; 177(3-4):371-3. DOI:10.1016/j.vetpar.2010.12.004 · 2.55 Impact Factor
  • J Li · G H Zhao · F C Zou · X H Mo · Z G Yuan · L Ai · H L Li · Y B Weng · R Q Lin · X Q Zhu
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    ABSTRACT: The present study studied the genetic variation among Schistosoma japonicum isolates from different endemic regions in mainland China and examined the phylogenetic relationships of zoonotic trematodes using the combined mitochondrial 16S and 12S ribosomal DNA sequences. The fragments of 16S and 12S rDNA were amplified from 22 S. japonicum isolates, and sequenced, and the relevant sequences of other nine trematode species belonging to six genera in four families were downloaded from GenBank, and their phylogenetic relationships were re-constructed by unweighted pair-group method with arithmetic averages analyses using the combined 16S and 12S rDNA sequences, with Trichinella spiralis as outgroup. The results showed that the partial sequences of mitochondrial 16S and 12S rDNA of S. japonicum were 757 and 797 bp, respectively, and they were quite conserved among the S. japonicum isolates. Phylogenetic analysis revealed that the combined 16S and 12S rDNA sequences were not able to distinguish S. japonicum isolates in mountainous areas from those in lake/marshland areas in mainland China. However, the combined sequences could distinguish different species of zoonotic trematodes. Therefore, the combined mitochondrial 16S and 12S rDNA sequences provide an effective molecular marker for the inter-species phylogenetic analysis and differential identification of zoonotic trematodes.
    Parasitology Research 05/2010; 107(3):561-9. DOI:10.1007/s00436-010-1895-x · 2.33 Impact Factor
  • W. Qin · C. Nam · H. L. Li · J. A. Szpunar
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    ABSTRACT: An in-depth understanding of the phase stability in ZrO2 film is very important for improving the corrosion resistance of zirconium alloys. ZrO2 film formed on Zr–2.5Nb alloys was characterized by grazing incidence X-ray diffraction. It is shown that in comparison to the monoclinic phase crystallites, the tetragonal phase crystallites undergo larger compressive strain, indicating that there are some regions of local stress concentration in ZrO2 film. These local stresses have an important influence on the phase stability in ZrO2 film. In this paper, the correlation among the macroscopic compressive stress, the grain size and the local stress was analyzed qualitatively from a thermodynamic viewpoint, showing that the tetragonal phase may be partly stabilized by the local stresses. The local stresses mainly come from three sources: the local transformation strain produced by the tetragonal→monoclinic phase transformation, the oxidization of intermetallic precipitates and the different matching degrees between the ZrO2 and Zr-based crystallites.
    Journal of Alloys and Compounds 06/2007; 437(1):280-284. DOI:10.1016/j.jallcom.2006.07.102 · 2.73 Impact Factor
  • W. Qin · C. Nam · H. L. Li · J. A. Szpunar
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    ABSTRACT: A thermodynamic model is developed to understand the origin of variation in the microstructure of ZrO2 film formed on zirconium alloys and its effects on corrosion resistance. The correlation among the tetragonal phase fraction, the stress (macroscopic and internal one), the ZrO2 grain size and the microstructural change of oxide film is formulized, and then analyzed. The results show that many complicated factors simultaneously govern the microstructure of oxide film. The tetragonal phase content near the oxide/metal interface, the macroscopic compressive stress near the interface, the decline gradient of macroscopic compressive stress and the internal stress induced by the transformation from the tetragonal to the monoclinic phase have very important influences on the transition from columnar grains to equiaxed grains, the crack formation and the degradation of oxidation resistance. The presence of intermetallic precipitates in oxide film may effectively relax the internal stress caused by transformation strain, stabilize the columnar-grain structure and reduce the probability of crack formation. How to reduce the transformation stress in the oxide film is a key to improve the corrosion resistance of zirconium alloys.
    Acta Materialia 03/2007; 55(5):1695-1701. DOI:10.1016/j.actamat.2006.10.030 · 4.47 Impact Factor
  • W. Qin · C. Nam · H.L. Li · J.A. Szpunar
    9th Internal Conference on CANDU fuel; 09/2005
  • Y. Cao · H.L. Li · J.A. Szpunar · W. Shmayda
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    ABSTRACT: The effects of Pd and Pd77Ag23 alloy membrane microstructures on hydrogen permeation have been investigated using the electrochemical permeation method. Deformation and annealing have been used to modify the membrane microstructures. The delay of hydrogen permeation in palladium increases when the degree of deformation is increases. The annealing of deformed palladium and Pd77Ag23 accelerates hydrogen diffusion. The annealed metals release hydrogen more rapidly than deformed metals. Also, the Pd77Ag23 alloy needs more time to release hydrogen than pure palladium. The solubility of hydrogen increases in both palladium and Pd77Ag23 when the amount of deformation is increased. A hydrogen trapping model was developed, which allows the prediction of trends of hydrogen permeation through membranes having different structures.
    Materials Science and Engineering A 08/2004; 379(1-2):173-180. DOI:10.1016/j.msea.2004.01.035 · 2.41 Impact Factor
  • M Liao · M. H. Huang · H L Li · J. A. Szpunar

Publication Stats

91 Citations
21.89 Total Impact Points

Institutions

  • 2010–2011
    • Yunnan Agricultural University
      Panlong, Shaanxi, China
    • South China Agricultural University
      • College of Veterinary Medicine
      Shengcheng, Guangdong, China
  • 2004–2007
    • McGill University
      • Department of Mining and Materials Engineering
      Montréal, Quebec, Canada