Publications (17)38.26 Total impact
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Article: The efficacy of MSC-HGF in treating pulmonary arterial hypertension (PAH) and connexin remodelling
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ABSTRACT: Abstract Background This study investigated whether the hepatocyte growth factor (HGF) genetically modified marrow-mesenchymal stem cells (MSCs) transplantation could offer a therapeutic benefit for pulmonary arterial hypertension (PAH). Methodology Three weeks after monocrotaline administration, Sprague-Dawley rats were randomly divided into the following groups: PAH (n=10), MSCs (5×106 MSCs injected into the jugular veins, n=10), HGF (5×106 MSCs transfected with Ad-HGF into the jugular veins, n=10). Another three weeks later, hemodynamic changes and histomorphology were observed. Electron microscopy and immunofluorescence were also used to observe changes in the gap junctions of the heart. Results Compared with the PAH and MSC groups, hemodynamic parameters improved significantly in the MSC-HGF group. Right ventricular hypertrophy was improved as measured by the RV/LV weight and thickness ratios. Histologically, cardiac myocytes and cell nuclei recovered and interstitial fibrosis decreased in the MSC and MSC-HGF groups. Under electron microscopy, the gap junctions exhibited a disorganised morphology in the PAH group and the number of gap junctions was lower in this group than in the other groups. The distribution of connexins 43 and 40 were improved in the MSC-HGF group. Conclusions MCT-induced PAH can be treated and improved by HGF genetically modified MSCs, which may occur via connexin remodeling.Central European Journal of Biology 03/2013; 8(3):240-251. · 1.00 Impact Factor -
Article: Simulated microgravity alters the metastatic potential of a human lung adenocarcinoma cell line.
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ABSTRACT: Simulated microgravity (SM) has been implicated in affecting diverse cellular pathways. Although there is emerging evidence that SM can alter cellular functions, its effect in cancer metastasis has not been addressed. Here, we demonstrate that SM inhibits migration, gelatinolytic activity, and cell proliferation of an A549 human lung adenocarcinoma cell line in vitro. Expression of antigen MKI67 and matrix metalloproteinase-2 (MMP2) was reduced in A549 cells stimulated by clinorotation when compared with the 1×g control condition, while overexpression of each gene improves ability of proliferation and migration, respectively, under SM conditions. These findings suggest that SM reduced the metastatic potential of human lung adenocarcinoma cells by altering the expression of MKI67 and MMP2, thereby inhibiting cell proliferation, migration, and invasion, which may provide some clues to study cancer metastasis in the future.In Vitro Cellular & Developmental Biology - Animal 02/2013; · 1.31 Impact Factor -
Article: Draft Genome Sequence of Escherichia coli Strain LCT-EC59.
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ABSTRACT: The space environment is a very special condition under which many organisms change many features. is employed widely as a prokaryotic model organism in the fields of biotechnology and microbiology. Here, we present the draft genome sequence of strain LCT-EC59 exposed to space conditions.Genome announcements. 01/2013; 1(1). -
Article: Draft Genome Sequences and Annotation of Enterococcus faecium Strain LCT-EF20.
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ABSTRACT: The space environment is reported to cause biological alterations in microorganisms, such as growth, drug resistance, and virulence. Here, we present the model of Enterococcus faecium to investigate the effects of space conditions on the microbe and on the whole-genome sequences of the strain LCT-EF20 after being exposed to space flight.Genome announcements. 01/2013; 1(1). -
Article: Draft Genome Sequences of the Enterococcus faecium Strain LCT-EF258.
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ABSTRACT: The space environment has been shown to affect microbes by altering various features, including morphology, growth rate, metabolism, virulence, drug resistance, and gene expression and mutation. Here we present the draft genome sequence of the Enterococcus faecium strain LCT-EF258, derived from the E. faecium strain CGMCC 1.1736, which was exposed to 17-day space flight.Genome announcements. 01/2013; 1(1). -
Article: Effect of artificial gravity with exercise training on lung function during head-down bed rest in humans.
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ABSTRACT: There is evidence to suggest that microgravity/weightlessness can induce changes in lung physiology/function. We hypothesized that microgravity, induced by head-down bed rest (HDBR), would induce changes in lung function and that exercise training with artificial gravity (AG) would prevent these changes from occurring. Twelve participants were randomly assigned to a control or AG exercise countermeasure (CM) group (n = 6 per group) and 96 h of 6° HDBR. Participants in the CM group were exposed to AG (alternating 2 min intervals of +1·0 and +2·0 G) for 30 min, twice daily, during which time ergometric exercise (40 W intensity) was performed. Pulse rate, oxygen saturation (SO(2) ) and lung function were measured and compared between groups. The CM and control groups were similar in mean age, height and weight. There were no significant within or between group differences over time in pulse rate, SO(2) , vital capacity, inspiratory capacity, tidal volume, expiratory reserve volume, inspiratory reserve volume, minute ventilation, forced vital capacity, forced expiratory volume in 1 s, peak expiratory flow, maximal expiratory flow in 25%, 50% and 75% vital capacity, forced inspiratory vital capacity, forced inspiratory volume in 1 s and maximal voluntary ventilation. Microgravity induced by 96 h of HDBR does not appear to affect lung function in humans. Further, AG with exercise training does not change lung function during 96 h of HDBR in humans.Clinical Physiology and Functional Imaging 01/2013; 33(1):24-9. · 1.33 Impact Factor -
Article: Genome sequence of Enterococcus faecium clinical isolate LCT-EF128.
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ABSTRACT: Enterococcus faecium, an opportunistic human pathogen that inhabits the gastrointestinal tracts of most mammals, has emerged as an important opportunistic nosocomial pathogen and is a prominent cause of multiresistant nosocomial infections. Here, we report the draft genome sequence of strain LCT-EF128, isolated from clinical specimens.Journal of bacteriology 09/2012; 194(17):4765. · 3.94 Impact Factor -
Article: Whole-genome sequence of Staphylococcus aureus strain LCT-SA112.
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ABSTRACT: Staphylococcus aureus is a facultative anaerobic Gram-positive coccal bacterium. S. aureus is the most common species of Staphylococcus to cause staphylococcal infections, which are very common in clinical medicine. Here we report the genome sequence of S. aureus strain LCT-SA112, which was isolated from S. aureus subsp. aureus CGMCC 1.230.Journal of bacteriology 08/2012; 194(15):4124. · 3.94 Impact Factor -
Article: Draft genome sequence of Escherichia coli LCT-EC106.
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ABSTRACT: Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the intestine of warm-blooded organisms. Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans. Here, we present the complete genome sequence of Escherichia coli LCT-EC106, which was isolated from CGMCC 1.2385.Journal of bacteriology 08/2012; 194(16):4443-4. · 3.94 Impact Factor -
Article: Draft genome sequence of Serratia marcescens strain LCT-SM213.
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ABSTRACT: Serratia marcescens is a species of Gram-negative, rod-shaped bacterium of the family Enterobacteriaceae. S. marcescens can cause nosocomial infections, particularly catheter-associated bacteremia, urinary tract infections, and wound infections. Here, we present the draft genome sequence of Serratia marcescens strain LCT-SM213, which was isolated from CGMCC 1.1857.Journal of bacteriology 08/2012; 194(16):4477-8. · 3.94 Impact Factor -
Article: Draft genome sequence of Pseudomonas aeruginosa strain ATCC 27853.
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ABSTRACT: Pseudomonas aeruginosa is a common bacterium that can cause disease. The versatility of Pseudomonas aeruginosa enables the organism to infect damaged tissues or those with reduced immunity which cause inflammation and sepsis. Here we report the genome sequence of the strain ATCC 27853.Journal of bacteriology 07/2012; 194(14):3755. · 3.94 Impact Factor -
Article: Draft genome sequence of Enterococcus faecium strain LCT-EF90.
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ABSTRACT: Enterococcus faecium is an opportunistic human pathogen, found widely in the human gastrointestinal tract, and can also be isolated from a variety of plants, animals, insects, and other environmental sources. Here, we present the fine draft genome sequence of E. faecium LCT-EF90.Journal of bacteriology 07/2012; 194(13):3556-7. · 3.94 Impact Factor -
Article: Draft genome sequence of Bacillus cereus strain LCT-BC244.
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ABSTRACT: Bacillus cereus is a prevalent, soil-dwelling, Gram-positive bacterium. Some strains are harmful to humans and cause food-borne illness, while other strains can be beneficial as probiotics for animals. To gain insight into the bacterial genetic determinants, we report the genome sequence of a strain, LCT-BC244, which was isolated from CGMCC 1.230.Journal of bacteriology 07/2012; 194(13):3549. · 3.94 Impact Factor -
Article: Whole-genome sequence of Klebsiella pneumonia strain LCT-KP214.
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ABSTRACT: Klebsiella pneumoniae is a gram-negative, nonmotile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium found in the normal flora of the mouth, skin, and intestines. Here we present the fine-draft genome sequence of K. pneumoniae strain LCT-KP214, which originated from K. pneumoniae strain CGMCC 1.1736.Journal of bacteriology 06/2012; 194(12):3281. · 3.94 Impact Factor -
Article: Assessment of the green florescence protein labeling method for tracking implanted mesenchymal stem cells.
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ABSTRACT: Although green fluorescent protein (GFP) labeling is widely accepted as a tracking method, much remains uncertain regarding the retention of injected GFP-labeled cells implanted in ischemic organs. In this study, we evaluate the effectiveness of GFP for identifying and tracking implanted bone marrow- mesenchymal stem cells (BM-MSCs) and the effect of GFP on the paracrine actions of these cells. MSCs isolated from rat femur marrow were transduced with a recombinant adenovirus carrying GFP. After transplantation of the GFP-labeled BM-MSCs into the infarct zone of rat hearts, the survival, distribution, and migration of the labeled cells were analyzed at 3, 7, 14, and 28 days. To evaluate the effect of GFP on the paracrine actions of BM-MSCs, Western blot analysis was performed to detect the expression of vascular endothelial growth factor (VEGF), b fibroblast growth factor (b FGF), tissue inhibitor of metalloproteinase-1 (TIMP-1) and matrix metalloproteinases-2 (MMP-2). GFP was successfully expressed by BM-MSCs in vitro. At 14 days after cell transplantation the GFP-positive cells could not be detected via confocal microscopy. By using a GFP antibody, distinct GFP-positive cells could be seen and quantitative analysis showed that the expression volume of GFP was 6.42 ± 0.92 mm(3) after 3 days, 1.24 ± 0.76 mm(3) after 7 days, 0.33 ± 0.03 mm(3) after 14 days, and 0.09 ± 0.05 mm(3) after 28 days. GFP labeling did not adversely affect the paracrine actions of BM-MSCs. GFP labeling could be used to track MSC distribution and their fate for at least 28 days after delivery to rat hearts with myocardial infarction, and this stem cell tracking strategy did not adversely affect the paracrine actions of BM-MSCs.Cytotechnology 02/2012; 64(4):391-401. · 1.21 Impact Factor -
Article: [Effect of combined therapy of granulocyte colony stimulating factor and bone marrow mesenchymal stem cells carrying hepatocyte growth factor gene on angiogenesis of myocardial infarction in rats].
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ABSTRACT: To investigate the effect of combined therapy of granulocyte colony stimulating factor (G-CSF) and bone marrow mesenchymal stem cells (BMSCs) carrying hepatocyte growth factor (HGF) gene on the angiogenesis of myocardial infarction (MI) in rats and the mechanisms of the synergistic effect. BMSCs were aspirated from the femur and tibia of 3-week-old Sprague Dawley (SD) male rats. The third generation of BMSCs were harvested and transfected with Ad-HGF. The MI models were established in 44 SD male rats (weighing 200-250 g) by ligating the left coronary artery. At 4 weeks after ligation, the shorting fraction (FS) of the left ventricle being below 30% was used as a criteria of model success. The BMSCs (5 x 10(7)/mL) transfected with Ad-HGF were transplanted into the infarct zone of 12 SD rats, and the expression of HGF protein was detected by Western blot method at 2, 7, and 14 days after transplantation. At 4 weeks, the other 32 SD rats were randomly divided into 4 groups (n = 8). The 0.1 mL normal saline was injected into the infarct zone in control group; 0.1 mL normal saline was injected combined with intraperitoneal injection G-CSF [100 microg/ (kg x d)] for 5 days in G-CSF group; 0.1 mL BMSCs (5 x 10(7)/mL) transfected with Ad-HGF was injected into the infarct zone in HGF group; 0.1 mL BMSCs (5 x 10(7)/mL) transfected with Ad-HGF was injected combined with intraperitoneal injection G-CSF [100 microg/ (kg x d)] for 5 days in combined therapy group. At 2 weeks after transplantation, heart function was detected by cardiac ultrasound and hemodynamic analysis, and then myocardial tissue was harvested to analyse the angiogenesis of the infarct zone, and the expression of VEGF protein by immunofluorescence staining. The expression of HGF protein in vivo was detected at 2 days and 7 days of BMSCs transfected with Ad-HGF transplantation. There was no significant difference in left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), dP/dtmax, and FS between G-CSF group and control group (P > 0.05). When compared with the control group, LVEDP decreased significantly; LVSP, FS, and dP/dtmax increased significantly (P < 0.05) in HGF group and combined therapy group. When compared with HGF group, FS and dP/dtmax increased significantly in combined therapy group (P < 0.05). Immunofluorescence staining showed that the vascular endothelial cells were observed in myocardial infarction border zone. The vascular density and the expression of VEGF protein were significantly higher in combined therapy group than in other 3 groups (P < 0.05). The combined therapy of G-CSF and BMSCs carrying HGF gene has a synergistic effect and can enhance infarct zone angiogenesis through inducing the expression of VEGF protein.Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 06/2011; 25(6):736-40. -
Article: Locally overexpressing hepatocyte growth factor prevents post-ischemic heart failure by inhibition of apoptosis via calcineurin-mediated pathway and angiogenesis.
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ABSTRACT: Myocardial infarction is a significant cause of heart failure. Currently, therapies are limited and novel revascularization methods may play a role. We investigated the effects of hepatocyte growth factor (HGF) expressed by bone marrow-derived mesenchymal stem cells (MSCs) on post-ischemic heart failure. Four weeks after myocardial infarction (MI), Sprague Dawley rats were randomly divided into saline control group, MSC-GFP group, MSC-HGF group, and MSC-HGF+CsA group. After another 4 weeks, hearts were analyzed for ventricular geometry, myocardial function, angiogenesis and endothelial cell density, apoptosis and the expression of calcineurin, Akt, and Bcl-2 protein. In MSC-HGF group, rats exhibited better LV systolic and diastolic function compared with other groups after 8 weeks of MI. Angiogenesis was significantly enhanced by HGF through inducing proliferation of endothelial cells. The effects of HGF on apoptosis were associated with the expression level of calcineurin protein. Our findings suggest that overexpression of HGF improved ischemic cardiac function through angiogenesis and reduction of apoptosis partly mediated by upregulation of calcineurin.Archives of Medical Research 03/2008; 39(2):179-88. · 1.88 Impact Factor
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Institutions
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2011–2013
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Chinese PLA General Hospital
Beijing, Beijing Shi, China
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2008
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Beijing Fuwai Hospital
Beijing, Beijing Shi, China
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